The DNA sequence of the selected gene is shown, including the ORF (underlined) and 300 nucleotides upstream of the ORF. All unique Sth1 Cas9 PAMs are highlighted according to predicted strength (blue=weak; red=strong; PAMscores derived from Bosch & DeJesus et al. 2021. Cell.). Note that the PAM is located on the complementary DNA strand (not listed), and thus highlighted PAM sequences are the reverse complements of the sequences recognized by Sth1 Cas9.
Note: Overlapping PAMs are shown in purple. Please mouse over the region to get information about which PAMs overlap.
The protein sequence of the selected gene is shown.