Track view

Track view is a dynamic overview of the fitness cost imposed by individual sgRNAs on the gene of your selection. The x-axis shows the genetic context of the selected gene as well as the potential operon structure it is part of. Each gene is color-coded by its TnSeq essentiality prediction (DeJesus et al. 2017). An arrow indicates the direction of gene expression.

The y-axis represents the sgRNA log2FC (L2FC) in the plus versus minus ATc samples. Each bar is an sgRNA color-coded by its predicted strength from blue (strength=0; weak) to red (strength=1; strong). The length of the bar indicates th maximum L2FC the sgRNA reaches over the course of the competitive growth experiment. Note that the exact number of generations needed to impose this fitness cost varies per sgRNA. By hovering over the sgRNAs, you can find detailed information on the L2FC over time.