1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	D	P	G	P	F	V	A	D	L	R	A	E	S	D	D	L	D	A	L	V	A	H	L	P	A	D	R	W	A	D	P	T	P	A	P	G	W	T	I	A	H	Q	I	G	H	L	L	W	T	D	R	V	A	L	T	A	V	T
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	E	A	G	F	A	E	L	M	T	A	A	A	A	N	P	A	G	F	V	D	D	A	A	T	E	L	A	A	V	S	P	A	E	L	L	T	D	W	R	V	T	R	G	R	L	H	E	E	L	L	A	V	P	D	G	R	K	L	A
Secondary structure
SS confidence
Disorder		?	?			?							?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		W	F	G	P	P	M	S	A	A	S	M	A	T	A	R	L	M	E	T	W	A	H	G	L	D	V	A	D	A	L	G	V	I	R	P	A	T	Q	R	L	R	S	I	A	H	L	G	V	R	T	R	D	Y	A	F	I	V	N	N	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		T	P	P	A	E	P	F	L	V	E	L	R	G	P	S	G	D	T	W	S	W	G	P	S	D	A	A	Q	R	V	T	G	S	A	E	D	F	C	F	L	V	T	Q	R	R	A	L	S	T	L	D	V	N	A	V	G	E	D	A	Q
Secondary structure
SS confidence
Disorder			?																																																?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.
Sequence		R	W	L	T	I	A	Q	A	F	A	G	P	P	G	R	G	R
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 7%) Alpha helix ( 57%) Beta strand ( 5%)

Rank Aligned region

PDB 2nsf chain A Region: 4 - 242 Aligned: 228 Modelled: 239 Confidence: 100.0% Identity: 15% PDB header:isomerase Chain: A: PDB Molecule:hypothetical protein cgl3021; PDBTitle: crystal structure of the mycothiol-dependent maleylpyruvate isomerase Phyre2 PDB 2nsf chain A domain 1 Region: 4 - 152 Aligned: 146 Modelled: 144 Confidence: 100.0% Identity: 13% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: Maleylpyruvate isomerase-like Phyre2 PDB 5civ chain A Region: 2 - 116 Aligned: 115 Modelled: 115 Confidence: 99.4% Identity: 13% PDB header:unknown function Chain: A: PDB Molecule:sibling bacteriocin; PDBTitle: sibling lethal factor precursor - dfsb Phyre2 PDB 5cog chain B Region: 2 - 116 Aligned: 115 Modelled: 115 Confidence: 99.4% Identity: 9% PDB header:unknown function Chain: B: PDB Molecule:irc4; PDBTitle: crystal structure of yeast irc4 Phyre2 PDB 4n6c chain B Region: 2 - 140 Aligned: 139 Modelled: 139 Confidence: 99.4% Identity: 6% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the b1rzq2 protein from streptococcus pneumoniae.2 northeast structural genomics consortium (nesg) target spr36. Phyre2 PDB 1rxq chain A Region: 5 - 148 Aligned: 141 Modelled: 144 Confidence: 99.1% Identity: 13% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: YfiT-like putative metal-dependent hydrolases Phyre2 PDB 5cof chain A Region: 5 - 116 Aligned: 112 Modelled: 112 Confidence: 99.0% Identity: 7% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterised protein q1r1x2 from escherichia2 coli uti89 Phyre2 PDB 6anr chain A Region: 5 - 116 Aligned: 112 Modelled: 112 Confidence: 99.0% Identity: 9% PDB header:hydrolase Chain: A: PDB Molecule:colibactin self-protection protein clbs; PDBTitle: crystal structure of a self resistance protein clbs from colibactin2 biosynthetic gene cluster Phyre2 PDB 2rd9 chain C Region: 3 - 148 Aligned: 143 Modelled: 144 Confidence: 98.9% Identity: 10% PDB header:hydrolase Chain: C: PDB Molecule:bh0186 protein; PDBTitle: crystal structure of a putative yfit-like metal-dependent hydrolase2 (bh0186) from bacillus halodurans c-125 at 2.30 a resolution Phyre2 PDB 6iz2 chain A Region: 1 - 156 Aligned: 135 Modelled: 135 Confidence: 98.9% Identity: 19% PDB header:unknown function Chain: A: PDB Molecule:dinb/yfit family protein; PDBTitle: crystal structure of dinb/yfit protein dr0053 from d. radiodurans r1 Phyre2 PDB 5cqv chain B Region: 2 - 116 Aligned: 115 Modelled: 115 Confidence: 98.9% Identity: 9% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterized protein q8dwv2 from streptococcus2 agalactiae Phyre2 PDB 3cex chain B Region: 7 - 146 Aligned: 140 Modelled: 140 Confidence: 98.8% Identity: 8% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the conserved protein of locus ef_3021 from2 enterococcus faecalis Phyre2 PDB 2ou6 chain A domain 1 Region: 5 - 147 Aligned: 143 Modelled: 143 Confidence: 98.7% Identity: 14% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 3dka chain A Region: 5 - 156 Aligned: 131 Modelled: 131 Confidence: 98.6% Identity: 10% PDB header:unknown function Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (yjoa, bsu12410) from2 bacillus subtilis at 2.30 a resolution Phyre2 PDB 3di5 chain A Region: 5 - 155 Aligned: 135 Modelled: 135 Confidence: 98.5% Identity: 14% PDB header:metal binding protein Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (bce_4655) from bacillus2 cereus atcc 10987 at 2.01 a resolution Phyre2 PDB 2yqy chain B Region: 3 - 150 Aligned: 121 Modelled: 125 Confidence: 98.5% Identity: 22% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein ttha0303; PDBTitle: crystal structure of tt2238, a four-helix bundle protein Phyre2 PDB 2qe9 chain B Region: 4 - 158 Aligned: 138 Modelled: 136 Confidence: 98.5% Identity: 11% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein yiza; PDBTitle: crystal structure of a putative metal-dependent hydrolase (yiza,2 bsu10800) from bacillus subtilis at 1.90 a resolution Phyre2 PDB 2p1a chain A domain 1 Region: 7 - 150 Aligned: 131 Modelled: 131 Confidence: 98.4% Identity: 11% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 3e4x chain B Region: 1 - 156 Aligned: 139 Modelled: 138 Confidence: 98.4% Identity: 10% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:apc36150; PDBTitle: crystal structure of putative metal-dependent hydrolases2 apc36150 Phyre2 PDB 2hkv chain A domain 1 Region: 7 - 150 Aligned: 128 Modelled: 128 Confidence: 98.0% Identity: 19% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 1 c2nsfA_ 2 d2nsfa1 3 c5civA_ 4 c5cogB_ 5 c4n6cB_ 6 d1rxqa_ 7 c5cofA_ 8 c6anrA_ 9 c2rd9C_ 10 c6iz2A_ 11 c5cqvB_ 12 c3cexB_ 13 d2ou6a1 14 c3dkaA_ 15 c3di5A_ 16 c2yqyB_ 17 c2qe9B_ 18 d2p1aa1 19 c3e4xB_ 20 d2hkva1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64