1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	A	E	Q	P	T	I	I	Y	T	L	T	D	E	A	P	L	L	A	T	Y	A	F	L	P	I	V	R	A	F	A	E	P	A	G	I	K	I	E	A	S	D	I	S	V	A	A	R	I	L	A	E	F	P	D	Y	L	T	E
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																														?										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		E	Q	R	V	P	D	N	L	A	E	L	G	R	L	T	Q	L	P	D	T	N	I	I	K	L	P	N	I	S	A	S	V	P	Q	L	V	A	A	I	K	E	L	Q	D	K	G	Y	A	V	P	D	Y	P	A	D	P	K	T	D	Q
Secondary structure
SS confidence
Disorder			?	?	?	?														?											?	?																	?							?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		E	K	A	I	K	E	R	Y	A	R	C	L	G	S	A	V	N	P	V	L	R	Q	G	N	S	D	R	R	A	P	K	A	V	K	E	Y	A	R	K	H	P	H	S	M	G	E	W	S	M	A	S	R	T	H	V	A	H	M	R	H
Secondary structure
SS confidence
Disorder																						?				?		?	?	?	?											?			?		?			?	?										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	D	F	Y	A	G	E	K	S	M	T	L	D	R	A	R	N	V	R	M	E	L	L	A	K	S	G	K	T	I	V	L	K	P	E	V	P	L	D	D	G	D	V	I	D	S	M	F	M	S	K	K	A	L	C	D	F	Y	E	E
Secondary structure
SS confidence
Disorder		?	?	?																									?				?	?	?	?			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Q	M	Q	D	A	F	E	T	G	V	M	F	S	L	H	V	K	A	T	M	M	K	V	S	H	P	I	V	F	G	H	A	V	R	I	F	Y	K	D	A	F	A	K	H	Q	E	L	F	D	D	L	G	V	N	V	N	N	G	L	S
Secondary structure
SS confidence
Disorder																					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		D	L	Y	S	K	I	E	S	L	P	A	S	Q	R	D	E	I	I	E	D	L	H	R	C	H	E	H	R	P	E	L	A	M	V	D	S	A	R	G	I	S	N	F	H	S	P	S	D	V	I	V	D	A	S	M	P	A	M	I	R
Secondary structure
SS confidence
Disorder									?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		A	G	G	K	M	Y	G	A	D	G	K	L	K	D	T	K	A	V	N	P	E	S	T	F	S	R	I	Y	Q	E	I	I	N	F	C	K	T	N	G	Q	F	D	P	T	T	M	G	T	V	P	N	V	G	L	M	A	Q	Q	A	E
Secondary structure
SS confidence
Disorder		?		?	?	?	?	?	?	?	?																																			?	?				?	?	?	?			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		E	Y	G	S	H	D	K	T	F	E	I	P	E	D	G	V	A	N	I	V	D	V	A	T	G	E	V	L	L	T	E	N	V	E	A	G	D	I	W	R	M	C	I	V	K	D	A	P	I	R	D	W	V	K	L	A	V	T	R	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		R	I	S	G	M	P	V	L	F	W	L	D	P	Y	R	P	H	E	N	E	L	I	K	K	V	K	T	Y	L	K	D	H	D	T	E	G	L	D	I	Q	I	M	S	Q	V	R	S	M	R	Y	T	C	E	R	L	V	R	G	L	D
Secondary structure
SS confidence
Disorder																																																										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		T	I	A	A	T	G	N	I	L	R	D	Y	L	T	D	L	F	P	I	L	E	L	G	T	S	A	K	M	L	S	V	V	P	L	M	A	G	G	G	M	Y	E	T	G	A	G	G	S	A	P	K	H	V	K	Q	L	V	E	E	N
Secondary structure
SS confidence
Disorder																									?			?						?										?	?	?			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660
Sequence		H	L	R	W	D	S	L	G	E	F	L	A	L	G	A	G	F	E	D	I	G	I	K	T	G	N	E	R	A	K	L	L	G	K	T	L	D	A	A	I	G	K	L	L	D	N	D	K	S	P	S	R	K	T	G	E	L	D	N	R
Secondary structure
SS confidence
Disorder																							?	?	?	?																				?	?		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.	680	.	.	.	.	.	.	.	.	.	690	.	.	.	.	.	.	.	.	.	700	.	.	.	.	.	.	.	.	.	710	.	.	.	.	.	.	.	.	.	720
Sequence		G	S	Q	F	Y	L	A	M	Y	W	A	Q	E	L	A	A	Q	T	D	D	Q	Q	L	A	E	H	F	A	S	L	A	D	V	L	T	K	N	E	D	V	I	V	R	E	L	T	E	V	Q	G	E	P	V	D	I	G	G	Y	Y	A
Secondary structure
SS confidence
Disorder																		?	?	?	?																											?	?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	730	.	.	.	.	.	.	.	.	.	740	.	.	.	.	.
Sequence		P	D	S	D	M	T	T	A	V	M	R	P	S	K	T	F	N	A	A	L	E	A	V	Q	G
Secondary structure
SS confidence
Disorder												?	?											?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 15%) Alpha helix ( 53%) Beta strand ( 11%)

Rank Aligned region

PDB 1itw chain A Region: 4 - 743 Aligned: 739 Modelled: 740 Confidence: 100.0% Identity: 61% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Monomeric isocitrate dehydrogenase Phyre2 PDB 6g3u chain A Region: 6 - 743 Aligned: 736 Modelled: 738 Confidence: 100.0% Identity: 68% PDB header:oxidoreductase Chain: A: PDB Molecule:isocitrate dehydrogenase; PDBTitle: structure of pseudomonas aeruginosa isocitrate dehydrogenase, idh Phyre2 PDB 2b0t chain A Region: 6 - 743 Aligned: 735 Modelled: 738 Confidence: 100.0% Identity: 58% PDB header:oxidoreductase Chain: A: PDB Molecule:nadp isocitrate dehydrogenase; PDBTitle: structure of monomeric nadp isocitrate dehydrogenase Phyre2 PDB 1lwd chain A Region: 421 - 648 Aligned: 223 Modelled: 228 Confidence: 99.3% Identity: 15% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 1zor chain B Region: 421 - 648 Aligned: 213 Modelled: 228 Confidence: 99.1% Identity: 16% PDB header:oxidoreductase Chain: B: PDB Molecule:isocitrate dehydrogenase; PDBTitle: isocitrate dehydrogenase from the hyperthermophile thermotoga maritima Phyre2 PDB 3us8 chain A Region: 178 - 648 Aligned: 232 Modelled: 245 Confidence: 98.2% Identity: 22% PDB header:oxidoreductase Chain: A: PDB Molecule:isocitrate dehydrogenase [nadp]; PDBTitle: crystal structure of an isocitrate dehydrogenase from sinorhizobium2 meliloti 1021 Phyre2 PDB 3blx chain L Region: 449 - 650 Aligned: 180 Modelled: 202 Confidence: 97.8% Identity: 17% PDB header:oxidoreductase Chain: L: PDB Molecule:isocitrate dehydrogenase [nad] subunit 2; PDBTitle: yeast isocitrate dehydrogenase (apo form) Phyre2 PDB 1wpw chain A Region: 389 - 655 Aligned: 250 Modelled: 267 Confidence: 97.8% Identity: 16% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 3blx chain M Region: 449 - 655 Aligned: 190 Modelled: 207 Confidence: 97.7% Identity: 12% PDB header:oxidoreductase Chain: M: PDB Molecule:isocitrate dehydrogenase [nad] subunit 1; PDBTitle: yeast isocitrate dehydrogenase (apo form) Phyre2 PDB 1t0l chain A Region: 178 - 648 Aligned: 237 Modelled: 245 Confidence: 97.6% Identity: 25% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 5grh chain B Region: 449 - 655 Aligned: 188 Modelled: 207 Confidence: 97.5% Identity: 13% PDB header:oxidoreductase Chain: B: PDB Molecule:isocitrate dehydrogenase [nad] subunit gamma, PDBTitle: crystal structure of the alpha gamma heterodimer of human idh3 in2 complex with mg(2+) Phyre2 PDB 5hn6 chain A Region: 314 - 659 Aligned: 290 Modelled: 315 Confidence: 97.4% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:homoisocitrate dehydrogenase; PDBTitle: crystal structure of beta-decarboxylating dehydrogenase (tk0280) from2 thermococcus kodakarensis complexed with mn and 3-isopropylmalate Phyre2 PDB 2uxq chain B Region: 421 - 646 Aligned: 219 Modelled: 226 Confidence: 97.4% Identity: 15% PDB header:oxidoreductase Chain: B: PDB Molecule:isocitrate dehydrogenase native; PDBTitle: isocitrate dehydrogenase from the psychrophilic bacterium2 desulfotalea psychrophila: biochemical properties and3 crystal structure analysis Phyre2 PDB 5grh chain A Region: 449 - 659 Aligned: 190 Modelled: 211 Confidence: 97.3% Identity: 13% PDB header:oxidoreductase Chain: A: PDB Molecule:isocitrate dehydrogenase [nad] subunit alpha, PDBTitle: crystal structure of the alpha gamma heterodimer of human idh3 in2 complex with mg(2+) Phyre2 PDB 2e0c chain A Region: 389 - 661 Aligned: 253 Modelled: 267 Confidence: 97.3% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:409aa long hypothetical nadp-dependent isocitrate PDBTitle: crystal structure of isocitrate dehydrogenase from sulfolobus tokodaii2 strain7 at 2.0 a resolution Phyre2 PDB 2qfy chain E Region: 429 - 655 Aligned: 218 Modelled: 227 Confidence: 97.0% Identity: 14% PDB header:oxidoreductase Chain: E: PDB Molecule:isocitrate dehydrogenase [nadp]; PDBTitle: crystal structure of saccharomyces cerevesiae mitochondrial nadp(+)-2 dependent isocitrate dehydrogenase in complex with a-ketoglutarate Phyre2 PDB 3fmx chain X Region: 458 - 651 Aligned: 176 Modelled: 194 Confidence: 96.9% Identity: 15% PDB header:oxidoreductase Chain: X: PDB Molecule:tartrate dehydrogenase/decarboxylase; PDBTitle: crystal structure of tartrate dehydrogenase from pseudomonas putida2 complexed with nadh Phyre2 PDB 1g2u chain A Region: 389 - 650 Aligned: 243 Modelled: 262 Confidence: 96.7% Identity: 20% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 1w0d chain A Region: 449 - 657 Aligned: 190 Modelled: 209 Confidence: 96.6% Identity: 18% Fold: Isocitrate/Isopropylmalate dehydrogenase-like Superfamily: Isocitrate/Isopropylmalate dehydrogenase-like Family: Dimeric isocitrate & isopropylmalate dehydrogenases Phyre2 PDB 2d1c chain B Region: 449 - 662 Aligned: 193 Modelled: 214 Confidence: 96.6% Identity: 16% PDB header:oxidoreductase Chain: B: PDB Molecule:isocitrate dehydrogenase; PDBTitle: crystal structure of tt0538 protein from thermus thermophilus hb8 Phyre2 1 d1itwa_ 2 c6g3uA_ 3 c2b0tA_ 4 d1lwda_ 5 c1zorB_ 6 c3us8A_ 7 c3blxL_ 8 d1wpwa_ 9 c3blxM_ 10 d1t0la_ 11 c5grhB_ 12 c5hn6A_ 13 c2uxqB_ 14 c5grhA_ 15 c2e0cA_ 16 c2qfyE_ 17 c3fmxX_ 18 d1g2ua_ 19 d1w0da_ 20 c2d1cB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106