1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	A	A	P	T	A	G	G	V	V	T	S	G	V	G	V	A	G	V	G	V	G	L	L	G	M	F	G	P	V	R	V	V	H	V	G	W	L	L	P	L	S	G	V	H	I	E	L	D	R	L	G	G	F	F	M	A	L	T
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	A	V	A	A	P	V	G	C	Y	L	I	G	Y	V	R	R	E	H	L	G	R	V	P	M	A	V	V	P	L	F	V	A	A	M	L	L	V	P	A	A	G	S	V	T	T	F	L	L	A	W	E	L	M	A	I	A	S	L	I
Secondary structure
SS confidence
Disorder																		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	V	L	S	E	H	A	R	P	Q	V	R	S	A	G	L	W	Y	A	V	M	T	Q	L	G	F	I	A	I	L	V	G	L	V	V	L	A	A	A	G	G	S	D	R	F	A	G	L	G	A	V	C	D	G	V	R	A	A	V	F
Secondary structure
SS confidence
Disorder																																																			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		M	L	T	L	V	G	F	G	S	K	A	G	L	V	P	L	H	A	W	L	P	R	A	H	P	E	A	P	S	P	V	S	A	L	M	S	A	A	M	V	N	L	G	I	Y	G	I	V	R	F	D	L	Q	L	L	G	P	G	P	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		W	W	G	L	A	L	L	A	V	G	G	T	S	A	L	Y	G	V	L	Q	A	S	V	A	A	D	L	K	R	L	L	A	Y	S	T	T	E	N	M	G	L	I	T	L	A	L	G	A	A	T	L	F	A	D	T	G	A	Y	G	P
Secondary structure
SS confidence
Disorder																																																				?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	S	I	A	A	A	A	A	M	L	H	M	I	A	H	A	A	F	K	S	L	A	F	M	A	A	G	S	V	L	A	A	T	G	L	R	D	L	D	L	L	G	G	L	A	R	R	M	P	A	T	T	V	F	F	G	V	A	A	L
Secondary structure
SS confidence
Disorder																																										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		G	A	C	G	L	P	L	G	A	G	F	V	S	E	W	L	L	V	Q	S	L	I	H	A	A	P	G	H	D	P	I	V	A	L	T	T	P	L	A	V	G	V	V	A	L	A	T	G	L	S	V	A	A	M	T	K	A	F	G	I
Secondary structure
SS confidence
Disorder																											?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		G	F	L	A	R	P	R	S	T	Q	A	E	A	A	R	E	A	P	A	S	M	R	A	G	M	A	I	A	A	G	A	C	L	V	L	A	V	A	P	L	L	V	A	P	M	V	R	R	A	A	A	T	L	P	A	A	Q	A	V	K
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?																																												?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		F	T	G	L	G	A	V	V	R	L	P	A	M	S	G	S	I	A	P	G	V	I	A	A	A	V	L	A	A	A	L	A	V	A	V	L	A	R	W	R	F	R	R	R	P	A	P	A	R	L	P	L	W	A	C	G	A	A	D	L
Secondary structure
SS confidence
Disorder						?	?	?				?	?	?	?	?	?	?	?																								?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		T	V	R	M	Q	Y	T	A	T	S	F	A	E	P	L	Q	R	V	F	G	D	V	L	R	P	D	T	D	I	E	V	T	H	T	A	E	S	R	Y	M	A	E	R	I	T	Y	R	T	A	V	A	D	A	I	E	Q	R	L	Y	T
Secondary structure
SS confidence
Disorder																																															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640
Sequence		P	V	V	G	A	V	A	A	M	A	E	L	L	R	R	A	H	T	G	S	V	H	R	Y	L	A	Y	G	A	L	G	V	L	I	V	L	V	V	A	R
Secondary structure
SS confidence
Disorder																																							?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 8%) Alpha helix ( 75%) Beta strand ( 1%) TM helix ( 46%) (View membrane topology prediction)

Rank Aligned region

PDB 4hea chain T Region: 4 - 640 Aligned: 574 Modelled: 601 Confidence: 100.0% Identity: 26% PDB header:oxidoreductase Chain: T: PDB Molecule:nadh-quinone oxidoreductase subunit 12; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 3rko chain L Region: 4 - 639 Aligned: 565 Modelled: 586 Confidence: 100.0% Identity: 21% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-quinone oxidoreductase subunit l; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 6hum chain F Region: 2 - 639 Aligned: 561 Modelled: 580 Confidence: 100.0% Identity: 22% PDB header:proton transport Chain: F: PDB Molecule:nadh dehydrogenase subunit 5; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6gcs chain 5 Region: 4 - 638 Aligned: 576 Modelled: 586 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: 5: PDB Molecule:nd5 subunit (nu5m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6g2j chain L Region: 5 - 638 Aligned: 563 Modelled: 574 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 5ldw chain L Region: 4 - 640 Aligned: 566 Modelled: 577 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 3rko chain M Region: 4 - 475 Aligned: 448 Modelled: 472 Confidence: 100.0% Identity: 19% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit m; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 6hum chain B Region: 17 - 474 Aligned: 437 Modelled: 437 Confidence: 100.0% Identity: 19% PDB header:proton transport Chain: B: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 2; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6gcs chain 4 Region: 11 - 471 Aligned: 435 Modelled: 461 Confidence: 100.0% Identity: 19% PDB header:oxidoreductase Chain: 4: PDB Molecule:nd4 subunit (nu4m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6hum chain D Region: 2 - 473 Aligned: 448 Modelled: 469 Confidence: 100.0% Identity: 21% PDB header:proton transport Chain: D: PDB Molecule:nad(p)h-quinone oxidoreductase chain 4 1; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6cfw chain H Region: 4 - 484 Aligned: 465 Modelled: 481 Confidence: 100.0% Identity: 26% PDB header:membrane protein Chain: H: PDB Molecule:monovalent cation/h+ antiporter subunit d; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 4he8 chain M Region: 4 - 476 Aligned: 439 Modelled: 473 Confidence: 100.0% Identity: 25% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit 13; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 3rko chain N Region: 4 - 471 Aligned: 436 Modelled: 468 Confidence: 100.0% Identity: 20% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-quinone oxidoreductase subunit n; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 5ldw chain M Region: 16 - 466 Aligned: 427 Modelled: 451 Confidence: 100.0% Identity: 20% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-ubiquinone oxidoreductase chain 4; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 4he8 chain I Region: 19 - 469 Aligned: 402 Modelled: 437 Confidence: 100.0% Identity: 25% PDB header:oxidoreductase Chain: I: PDB Molecule:nadh-quinone oxidoreductase subunit 14; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 6gcs chain 2 Region: 13 - 467 Aligned: 423 Modelled: 455 Confidence: 100.0% Identity: 13% PDB header:oxidoreductase Chain: 2: PDB Molecule:nd2 subunit (nu2m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 5xtd chain I Region: 80 - 463 Aligned: 341 Modelled: 369 Confidence: 100.0% Identity: 16% PDB header:oxidoreductase/electron transport Chain: I: PDB Molecule:nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit PDBTitle: cryo-em structure of human respiratory complex i Phyre2 PDB 6g2j chain N Region: 80 - 462 Aligned: 340 Modelled: 368 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-ubiquinone oxidoreductase chain 2; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 5ldw chain N Region: 80 - 461 Aligned: 339 Modelled: 367 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-ubiquinone oxidoreductase chain 2; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 5lc5 chain H Region: 106 - 206 Aligned: 99 Modelled: 101 Confidence: 92.5% Identity: 12% PDB header:oxidoreductase Chain: H: PDB Molecule:nadh-ubiquinone oxidoreductase chain 1; PDBTitle: structure of mammalian respiratory complex i, class2 Phyre2 1 c4heaT_ 2 c3rkoL_ 3 c6humF_ 4 c6gcs5_ 5 c6g2jL_ 6 c5ldwL_ 7 c3rkoM_ 8 c6humB_ 9 c6gcs4_ 10 c6humD_ 11 c6cfwH_ 12 c4he8M_ 13 c3rkoN_ 14 c5ldwM_ 15 c4he8I_ 16 c6gcs2_ 17 c5xtdi_ 18 c6g2jN_ 19 c5ldwN_ 20 c5lc5H_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99