1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	Y	L	A	G	A	A	Q	I	G	G	V	M	V	G	A	P	L	V	I	G	M	T	R	Q	V	R	A	R	W	E	G	R	A	G	A	G	L	L	Q	P	W	R	D	L	L	K	Q	L	G	K	Q	Q	I	T	P	A	G	T
Secondary structure
SS confidence
Disorder		?	?																																																			?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	I	V	F	A	A	A	P	V	I	V	A	G	T	T	L	L	I	A	A	I	A	P	L	V	A	T	G	S	P	L	D	P	S	A	D	L	F	A	V	V	G	L	L	F	L	G	T	V	A	L	T	L	A	G	I	D	T	G	T
Secondary structure
SS confidence
Disorder																											?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	F	G	G	M	G	A	S	R	E	I	T	I	A	A	L	V	E	P	T	I	L	L	A	V	F	A	L	S	I	P	A	G	S	A	N	L	G	A	L	V	A	S	T	I	D	H	P	G	H	V	V	S	L	A	G	V	L	A	F
Secondary structure
SS confidence
Disorder						?																																								?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	A	L	V	I	V	I	V	A	E	T	G	R	L	P	V	D	N	P	A	T	H	L	E	L	T	M	V	H	E	A	M	V	L	E	Y	A	G	P	R	L	A	L	V	E	W	A	A	G	M	R	L	T	V	L	L	A	L	L	A
Secondary structure
SS confidence
Disorder												?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		N	L	F	L	P	W	G	I	A	G	A	A	P	T	A	L	D	V	L	T	G	V	V	A	V	A	A	K	V	A	I	L	A	V	L	L	A	T	F	E	V	F	L	A	K	L	R	L	F	R	V	P	E	L	L	A	G	S	F	L
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.
Sequence		L	A	L	L	A	V	T	A	A	N	F	F	T	V	G	A
Secondary structure
SS confidence
Disorder															?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 9%) Alpha helix ( 78%) Beta strand ( 0%) TM helix ( 60%) (View membrane topology prediction)

Rank Aligned region

PDB 6gcs chain 1 Region: 1 - 314 Aligned: 308 Modelled: 314 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: 1: PDB Molecule:nd1 subunit (nu1m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6hum chain A Region: 3 - 314 Aligned: 305 Modelled: 312 Confidence: 100.0% Identity: 21% PDB header:proton transport Chain: A: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 1; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6cfw chain M Region: 1 - 315 Aligned: 308 Modelled: 315 Confidence: 100.0% Identity: 22% PDB header:membrane protein Chain: M: PDB Molecule:mbh13 nadh dehydrogenase subunit; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 5lc5 chain H Region: 7 - 313 Aligned: 294 Modelled: 307 Confidence: 100.0% Identity: 21% PDB header:oxidoreductase Chain: H: PDB Molecule:nadh-ubiquinone oxidoreductase chain 1; PDBTitle: structure of mammalian respiratory complex i, class2 Phyre2 PDB 4he8 chain H Region: 34 - 314 Aligned: 252 Modelled: 260 Confidence: 100.0% Identity: 21% PDB header:oxidoreductase Chain: H: PDB Molecule:nadh-quinone oxidoreductase subunit 8; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 4hea chain T Region: 76 - 204 Aligned: 127 Modelled: 129 Confidence: 89.1% Identity: 13% PDB header:oxidoreductase Chain: T: PDB Molecule:nadh-quinone oxidoreductase subunit 12; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 3rko chain L Region: 76 - 204 Aligned: 128 Modelled: 129 Confidence: 87.2% Identity: 15% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-quinone oxidoreductase subunit l; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 5ldw chain L Region: 97 - 204 Aligned: 100 Modelled: 108 Confidence: 73.9% Identity: 12% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 6gcs chain 5 Region: 97 - 197 Aligned: 97 Modelled: 101 Confidence: 65.3% Identity: 18% PDB header:oxidoreductase Chain: 5: PDB Molecule:nd5 subunit (nu5m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6gcs chain 4 Region: 93 - 214 Aligned: 116 Modelled: 122 Confidence: 61.7% Identity: 16% PDB header:oxidoreductase Chain: 4: PDB Molecule:nd4 subunit (nu4m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6hum chain F Region: 58 - 198 Aligned: 131 Modelled: 141 Confidence: 57.4% Identity: 11% PDB header:proton transport Chain: F: PDB Molecule:nadh dehydrogenase subunit 5; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6g2j chain L Region: 97 - 197 Aligned: 93 Modelled: 101 Confidence: 54.1% Identity: 17% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 1q2i chain A Region: 37 - 52 Aligned: 16 Modelled: 16 Confidence: 47.4% Identity: 44% PDB header:antitumor protein Chain: A: PDB Molecule:pnc27; PDBTitle: nmr solution structure of a peptide from the mdm-2 binding2 domain of the p53 protein that is selectively cytotoxic to3 cancer cells Phyre2 PDB 6gcs chain 2 Region: 78 - 197 Aligned: 114 Modelled: 120 Confidence: 43.6% Identity: 12% PDB header:oxidoreductase Chain: 2: PDB Molecule:nd2 subunit (nu2m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 5ldw chain N Region: 93 - 204 Aligned: 98 Modelled: 98 Confidence: 32.1% Identity: 12% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-ubiquinone oxidoreductase chain 2; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 4he8 chain I Region: 92 - 243 Aligned: 136 Modelled: 152 Confidence: 30.1% Identity: 21% PDB header:oxidoreductase Chain: I: PDB Molecule:nadh-quinone oxidoreductase subunit 14; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 6o7u chain A Region: 37 - 199 Aligned: 149 Modelled: 163 Confidence: 27.5% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule: PDBTitle: saccharomyces cerevisiae v-atpase stv1-vo Phyre2 PDB 2jpk chain A Region: 38 - 55 Aligned: 18 Modelled: 18 Confidence: 26.9% Identity: 28% PDB header:antimicrobial protein Chain: A: PDB Molecule:bacteriocin lactococcin-g subunit beta; PDBTitle: lactococcin g-b in dpc Phyre2 PDB 1xn8 chain A Region: 196 - 233 Aligned: 38 Modelled: 38 Confidence: 26.8% Identity: 21% Fold: Hypothetical protein YqbG Superfamily: Hypothetical protein YqbG Family: Hypothetical protein YqbG Phyre2 PDB 1lbq chain A Region: 32 - 53 Aligned: 22 Modelled: 22 Confidence: 24.3% Identity: 18% Fold: Chelatase-like Superfamily: Chelatase Family: Ferrochelatase Phyre2 1 c6gcs1_ 2 c6humA_ 3 c6cfwM_ 4 c5lc5H_ 5 c4he8H_ 6 c4heaT_ 7 c3rkoL_ 8 c5ldwL_ 9 c6gcs5_ 10 c6gcs4_ 11 c6humF_ 12 c6g2jL_ 13 c1q2iA_ 14 c6gcs2_ 15 c5ldwN_ 16 c4he8I_ 17 c6o7ua_ 18 c2jpkA_ 19 d1xn8a_ 20 d1lbqa_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51