1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	N	A	N	F	S	I	L	V	D	F	A	A	G	G	L	V	L	A	S	V	L	I	V	W	R	R	D	L	R	A	I	V	R	L	L	A	W	Q	G	A	A	L	A	A	I	P	L	L	R	G	I	R	D	N	D	R	A	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	A	V	G	I	A	V	L	A	L	R	A	L	V	L	P	W	L	L	A	R	A	V	G	A	E	A	A	A	Q	R	E	A	T	P	L	V	N	T	A	S	S	L	L	I	T	A	G	L	T	L	T	A	F	A	I	T	Q	P	V
Secondary structure
SS confidence
Disorder																												?		?	?						?		?																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	N	L	E	P	G	V	T	I	N	A	V	P	A	A	F	A	V	V	L	I	A	L	F	V	M	T	T	R	L	H	A	V	S	Q	A	A	G	F	L	M	L	D	N	G	I	A	A	T	A	F	L	L	T	A	G	V	P	L	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?																																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220
Sequence		V	E	L	G	A	S	L	D	V	L	F	A	V	I	V	I	G	V	L	T	G	R	L	R	R	I	F	G	D	A	D	L	D	K	L	R	E	L	R	D
Secondary structure
SS confidence
Disorder																																						?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 12%) Alpha helix ( 87%) Beta strand ( 0%) TM helix ( 61%) (View membrane topology prediction)

Rank Aligned region

PDB 4hea chain K Region: 130 - 219 Aligned: 90 Modelled: 90 Confidence: 98.6% Identity: 17% PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-quinone oxidoreductase subunit 11; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 6hum chain E Region: 129 - 219 Aligned: 91 Modelled: 91 Confidence: 98.5% Identity: 13% PDB header:proton transport Chain: E: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 4l; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 3rko chain K Region: 127 - 219 Aligned: 92 Modelled: 93 Confidence: 98.3% Identity: 17% PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-quinone oxidoreductase subunit k; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 6cfw chain G Region: 131 - 217 Aligned: 87 Modelled: 87 Confidence: 98.1% Identity: 18% PDB header:membrane protein Chain: G: PDB Molecule:monovalent cation/h+ antiporter subunit c; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 6gcs chain L Region: 132 - 169 Aligned: 38 Modelled: 38 Confidence: 62.3% Identity: 8% PDB header:oxidoreductase Chain: L: PDB Molecule:nd4l subunit (nulm); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 5ldw chain M Region: 80 - 220 Aligned: 136 Modelled: 141 Confidence: 60.3% Identity: 8% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-ubiquinone oxidoreductase chain 4; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 6cfw chain D Region: 12 - 52 Aligned: 41 Modelled: 41 Confidence: 40.0% Identity: 29% PDB header:membrane protein Chain: D: PDB Molecule:mbh subunit; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 5us5 chain B Region: 151 - 161 Aligned: 11 Modelled: 11 Confidence: 25.2% Identity: 27% PDB header:structural genomics Chain: B: PDB Molecule:upf0297 protein ef_1202; PDBTitle: solution structure of the ireb homodimer Phyre2 PDB 3rko chain N Region: 27 - 219 Aligned: 188 Modelled: 193 Confidence: 15.8% Identity: 14% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-quinone oxidoreductase subunit n; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 2jp3 chain A Region: 118 - 150 Aligned: 33 Modelled: 33 Confidence: 11.9% Identity: 3% PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles Phyre2 PDB 2jo1 chain A Region: 117 - 150 Aligned: 34 Modelled: 34 Confidence: 11.5% Identity: 12% PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles Phyre2 PDB 3rko chain M Region: 16 - 220 Aligned: 194 Modelled: 198 Confidence: 11.2% Identity: 13% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit m; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 4pde chain A Region: 147 - 164 Aligned: 18 Modelled: 18 Confidence: 8.9% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:protein fdhd; PDBTitle: crystal structure of fdhd in complex with gdp Phyre2 PDB 2mkv chain A Region: 119 - 150 Aligned: 32 Modelled: 32 Confidence: 8.6% Identity: 16% PDB header:transport protein Chain: A: PDB Molecule:sodium/potassium-transporting atpase subunit gamma; PDBTitle: structure of the na,k-atpase regulatory protein fxyd2b in micelles Phyre2 PDB 6hum chain D Region: 92 - 220 Aligned: 126 Modelled: 129 Confidence: 7.7% Identity: 9% PDB header:proton transport Chain: D: PDB Molecule:nad(p)h-quinone oxidoreductase chain 4 1; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 4hea chain T Region: 91 - 220 Aligned: 128 Modelled: 130 Confidence: 7.6% Identity: 9% PDB header:oxidoreductase Chain: T: PDB Molecule:nadh-quinone oxidoreductase subunit 12; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 2n2a chain A Region: 178 - 202 Aligned: 25 Modelled: 25 Confidence: 5.1% Identity: 28% PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-2; PDBTitle: spatial structure of her2/erbb2 dimeric transmembrane domain in the2 presence of cytoplasmic juxtamembrane domains Phyre2 1 c4heaK_ 2 c6humE_ 3 c3rkoK_ 4 c6cfwG_ 5 c6gcsL_ 6 c5ldwM_ 7 c6cfwD_ 8 c5us5B_ 9 c3rkoN_ 10 c2jp3A_ 11 c2jo1A_ 12 c3rkoM_ 13 c4pdeA_ 14 c2mkvA_ 15 c6humD_ 16 c4heaT_ 17 c2n2aA_