1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	M	S	A	S	W	L	R	H	R	V	S	E	R	G	L	I	A	T	A	E	Q	L	W	A	D	S	F	R	L	A	L	V	A	A	H	D	D	G	D	S	L	R	V	V	Y	L	F	L	A	G	Y	P	D	R	R	V	E	L	E
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Y	V	V	P	A	D	N	P	E	I	R	S	L	A	Y	L	S	F	P	A	G	R	F	E	R	E	M	A	D	L	Y	G	I	R	P	V	G	H	P	K	P	R	R	L	V	R	H	A	H	W	P	D	W	H	P	M	R	T	D	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	P	A	P	E	F	T	D	T	G	A	F	P	F	L	A	V	E	G	P	G	V	Y	E	I	P	V	G	P	V	H	A	G	L	I	E	P	G	H	F	R	F	S	V	A	G	E	T	I	V	R	L	K	A	R	L	W	F	V	H
Secondary structure
SS confidence
Disorder			?	?	?	?			?	?				?				?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		R	G	I	E	K	L	F	H	G	R	P	A	T	A	A	V	D	L	A	E	R	I	S	G	D	T	S	A	A	H	A	L	A	H	S	L	A	I	E	D	A	L	G	I	E	L	P	H	E	V	H	R	L	R	A	L	I	V	E	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		E	R	L	Y	N	H	A	A	D	L	G	A	L	A	N	D	V	G	Y	S	L	A	N	A	H	A	Q	R	I	R	E	N	L	L	R	R	N	A	A	V	T	G	H	R	L	L	R	G	A	I	R	A	G	G	V	A	L	R	A	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		P	D	T	D	E	L	A	A	L	A	V	D	L	A	E	V	A	T	L	T	L	A	N	S	V	V	Y	D	R	F	A	G	T	A	V	L	H	P	D	D	A	S	A	L	G	C	L	G	Y	V	A	R	A	S	G	L	R	S	D	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		R	V	E	H	P	T	I	V	L	P	I	T	E	I	G	A	P	D	G	D	V	L	A	R	Y	T	V	R	R	D	E	F	A	A	S	A	A	L	A	Q	H	I	V	E	S	H	T	G	P	I	E	Y	A	A	T	L	H	P	V	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		A	P	S	S	G	I	G	I	V	E	G	W	R	G	T	I	V	H	R	V	E	I	D	V	D	G	R	I	T	R	A	K	V	V	D	P	S	W	F	N	W	P	A	L	P	V	A	M	A	D	T	I	V	P	D	F	P	L	A	N
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.
Sequence		K	S	F	N	Q	S	Y	A	G	N	D	L
Secondary structure
SS confidence
Disorder										?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 4%) Alpha helix ( 39%) Beta strand ( 18%) TM helix ( 5%) (View membrane topology prediction)

Rank Aligned region

PDB 5lc5 chain D Region: 98 - 491 Aligned: 391 Modelled: 394 Confidence: 100.0% Identity: 21% PDB header:oxidoreductase Chain: D: PDB Molecule:nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, PDBTitle: structure of mammalian respiratory complex i, class2 Phyre2 PDB 6gcs chain C Region: 115 - 491 Aligned: 375 Modelled: 377 Confidence: 100.0% Identity: 23% PDB header:oxidoreductase Chain: C: PDB Molecule:49-kda protein (nucm); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6cfw chain L Region: 143 - 491 Aligned: 349 Modelled: 349 Confidence: 100.0% Identity: 29% PDB header:membrane protein Chain: L: PDB Molecule:membrane-bound hydrogenase subunit alpha; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 6hum chain H Region: 135 - 491 Aligned: 357 Modelled: 357 Confidence: 100.0% Identity: 24% PDB header:proton transport Chain: H: PDB Molecule:nad(p)h-quinone oxidoreductase subunit h; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 2fug chain 4 Region: 152 - 491 Aligned: 338 Modelled: 340 Confidence: 100.0% Identity: 27% PDB header:oxidoreductase Chain: 4: PDB Molecule:nadh-quinone oxidoreductase chain 4; PDBTitle: crystal structure of the hydrophilic domain of respiratory complex i2 from thermus thermophilus Phyre2 PDB 2fug chain 4 domain 1 Region: 152 - 491 Aligned: 338 Modelled: 340 Confidence: 100.0% Identity: 27% Fold: HydB/Nqo4-like Superfamily: HydB/Nqo4-like Family: Nqo4-like Phyre2 PDB 1e3d chain B Region: 136 - 490 Aligned: 352 Modelled: 355 Confidence: 100.0% Identity: 25% Fold: HydB/Nqo4-like Superfamily: HydB/Nqo4-like Family: Nickel-iron hydrogenase, large subunit Phyre2 PDB 1yq9 chain H domain 1 Region: 144 - 490 Aligned: 344 Modelled: 347 Confidence: 100.0% Identity: 25% Fold: HydB/Nqo4-like Superfamily: HydB/Nqo4-like Family: Nickel-iron hydrogenase, large subunit Phyre2 PDB 1h2a chain L Region: 142 - 490 Aligned: 346 Modelled: 349 Confidence: 100.0% Identity: 24% PDB header:oxidoreductase Chain: L: PDB Molecule:hydrogenase; PDBTitle: single crystals of hydrogenase from desulfovibrio vulgaris Phyre2 PDB 1frf chain L Region: 136 - 490 Aligned: 352 Modelled: 355 Confidence: 100.0% Identity: 25% Fold: HydB/Nqo4-like Superfamily: HydB/Nqo4-like Family: Nickel-iron hydrogenase, large subunit Phyre2 PDB 2wpn chain B Region: 141 - 490 Aligned: 342 Modelled: 350 Confidence: 100.0% Identity: 22% PDB header:oxidoreductase Chain: B: PDB Molecule:periplasmic [nifese] hydrogenase, large subunit, PDBTitle: structure of the oxidised, as-isolated nifese hydrogenase from d.2 vulgaris hildenborough Phyre2 PDB 6ehq chain M Region: 144 - 490 Aligned: 344 Modelled: 347 Confidence: 100.0% Identity: 24% PDB header:oxidoreductase Chain: M: PDB Molecule:hydrogenase-2 large chain; PDBTitle: e. coli hydrogenase-2 (as isolated form). Phyre2 PDB 1cc1 chain L Region: 143 - 490 Aligned: 341 Modelled: 348 Confidence: 100.0% Identity: 21% Fold: HydB/Nqo4-like Superfamily: HydB/Nqo4-like Family: Nickel-iron hydrogenase, large subunit Phyre2 PDB 1wui chain L domain 1 Region: 142 - 490 Aligned: 344 Modelled: 349 Confidence: 100.0% Identity: 26% Fold: HydB/Nqo4-like Superfamily: HydB/Nqo4-like Family: Nickel-iron hydrogenase, large subunit Phyre2 PDB 3zfs chain A Region: 145 - 490 Aligned: 344 Modelled: 346 Confidence: 100.0% Identity: 21% PDB header:oxidoreductase Chain: A: PDB Molecule:f420-reducing hydrogenase, subunit alpha; PDBTitle: cryo-em structure of the f420-reducing nife-hydrogenase from a2 methanogenic archaeon with bound substrate Phyre2 PDB 5aa5 chain C Region: 143 - 490 Aligned: 343 Modelled: 348 Confidence: 100.0% Identity: 22% PDB header:oxidoreductase Chain: C: PDB Molecule:nife-hydrogenase large subunit, hofg; PDBTitle: actinobacterial-type nife-hydrogenase from ralstonia eutropha h16 at2 2.85 angstrom resolution Phyre2 PDB 5odq chain F Region: 143 - 490 Aligned: 346 Modelled: 348 Confidence: 100.0% Identity: 21% PDB header:oxidoreductase Chain: F: PDB Molecule:methyl-viologen reducing hydrogenase, subunit a; PDBTitle: heterodisulfide reductase / [nife]-hydrogenase complex from2 methanothermococcus thermolithotrophicus soaked with3 bromoethanesulfonate. Phyre2 PDB 5xf9 chain H Region: 139 - 490 Aligned: 348 Modelled: 352 Confidence: 100.0% Identity: 19% PDB header:oxidoreductase Chain: H: PDB Molecule:nad-reducing hydrogenase; PDBTitle: crystal structure of nad+-reducing [nife]-hydrogenase in the air-2 oxidized state Phyre2 PDB 3myr chain B Region: 145 - 490 Aligned: 342 Modelled: 346 Confidence: 100.0% Identity: 27% PDB header:oxidoreductase Chain: B: PDB Molecule:nickel-dependent hydrogenase large subunit; PDBTitle: crystal structure of [nife] hydrogenase from allochromatium vinosum in2 its ni-a state Phyre2 PDB 5yy0 chain A Region: 149 - 490 Aligned: 339 Modelled: 341 Confidence: 100.0% Identity: 15% PDB header:metal binding protein Chain: A: PDB Molecule:cytosolic nife-hydrogenase, alpha subunit; PDBTitle: crystal structure of the hyhl-hypa complex (form ii) Phyre2 1 c5lc5D_ 2 c6gcsC_ 3 c6cfwL_ 4 c6humH_ 5 c2fug4_ 6 d2fug41 7 d1e3db_ 8 d1yq9h1 9 c1h2aL_ 10 d1frfl_ 11 c2wpnB_ 12 c6ehqM_ 13 d1cc1l_ 14 d1wuil1 15 c3zfsA_ 16 c5aa5C_ 17 c5odqF_ 18 c5xf9H_ 19 c3myrB_ 20 c5yy0A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99