1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	L	A	Q	A	T	T	A	G	S	F	N	H	H	A	S	T	V	L	Q	G	C	R	G	V	P	A	A	M	W	S	E	P	A	G	A	I	R	R	H	C	A	T	I	D	G	M	D	C	E	V	A	R	E	A	L	S	A	R	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?																			?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	G	E	R	A	P	V	P	S	A	R	V	D	E	H	L	G	E	C	S	A	C	R	A	W	F	T	Q	V	A	S	Q	A	G	D	L	R	R	L	A	E	S	R	P	V	V	P	P	V	G	R	L	G	I	R	R	A	P	R	R
Secondary structure
SS confidence
Disorder								?	?																														?	?	?	?	?	?	?	?	?	?						?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Q	H	S	P	M	T	W	R	R	W	A	L	L	C	V	G	I	A	Q	I	A	L	G	T	V	Q	G	F	G	L	D	V	G	L	T	H	Q	H	P	T	G	A	G	T	H	L	L	N	E	S	T	S	W	S	I	A	L	G	V	I
Secondary structure
SS confidence
Disorder		?	?	?																										?	?		?		?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		M	V	G	A	A	L	W	P	S	A	A	A	G	L	A	G	V	L	T	A	F	V	A	I	L	T	G	Y	V	I	V	D	A	L	S	G	A	V	S	T	T	R	I	L	T	H	L	P	V	V	I	G	A	V	L	A	I	M	V	W
Secondary structure
SS confidence
Disorder									?	?			?																							?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.
Sequence		R	S	A	S	G	P	R	P	R	P	D	A	V	A	A	E	P	D	I	V	L	P	D	N	A	S	R	G	R	R	R	G	H	L	W	P	T	D	G	S	A	A
Secondary structure
SS confidence
Disorder			?		?	?	?	?	?	?	?	?	?	?	?	?								?	?	?	?	?	?	?	?	?	?		?		?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 30%) Alpha helix ( 69%) Beta strand ( 0%) TM helix ( 26%) (View membrane topology prediction)

Rank Aligned region

PDB 5frh chain A Region: 40 - 115 Aligned: 76 Modelled: 76 Confidence: 99.3% Identity: 16% PDB header:transcription Chain: A: PDB Molecule:anti-sigma factor rsra; PDBTitle: solution structure of oxidised rsra Phyre2 PDB 5wuq chain D Region: 49 - 114 Aligned: 66 Modelled: 66 Confidence: 99.2% Identity: 20% PDB header:metal binding protein Chain: D: PDB Molecule:anti-sigma-w factor rsiw; PDBTitle: crystal structure of sigw in complex with its anti-sigma rsiw, a zinc2 binding form Phyre2 PDB 3hug chain J Region: 54 - 102 Aligned: 49 Modelled: 49 Confidence: 98.9% Identity: 20% PDB header:transcription/membrane protein Chain: J: PDB Molecule:probable conserved membrane protein; PDBTitle: crystal structure of mycobacterium tuberculosis anti-sigma factor rsla2 in complex with -35 promoter binding domain of sigl Phyre2 PDB 3vdo chain B Region: 51 - 117 Aligned: 67 Modelled: 67 Confidence: 98.7% Identity: 7% PDB header:dna binding protein/protein binding Chain: B: PDB Molecule:anti-sigma-k factor rska; PDBTitle: structure of extra-cytoplasmic function(ecf) sigma factor sigk in2 complex with its negative regulator rska from mycobacterium3 tuberculosis Phyre2 PDB 2z2s chain D Region: 54 - 127 Aligned: 74 Modelled: 74 Confidence: 98.5% Identity: 22% PDB header:transcription Chain: D: PDB Molecule:anti-sigma factor chrr, transcriptional activator chrr; PDBTitle: crystal structure of rhodobacter sphaeroides sige in complex with the2 anti-sigma chrr Phyre2 PDB 1or7 chain C Region: 53 - 119 Aligned: 64 Modelled: 67 Confidence: 96.4% Identity: 16% Fold: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Superfamily: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Family: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Phyre2 PDB 1or7 chain C Region: 53 - 119 Aligned: 64 Modelled: 67 Confidence: 96.4% Identity: 16% PDB header:transcription Chain: C: PDB Molecule:sigma-e factor negative regulatory protein; PDBTitle: crystal structure of escherichia coli sigmae with the cytoplasmic2 domain of its anti-sigma rsea Phyre2 PDB 6gh5 chain M Region: 2 - 78 Aligned: 74 Modelled: 77 Confidence: 24.3% Identity: 18% PDB header:transcription Chain: M: PDB Molecule:rna polymerase sigma-54 factor,rna polymerase sigma-54 PDBTitle: cryo-em structure of bacterial rna polymerase-sigma54 holoenzyme2 transcription open complex Phyre2 PDB 2muq chain A Region: 64 - 79 Aligned: 16 Modelled: 16 Confidence: 21.4% Identity: 31% PDB header:ubiquitin binding protein Chain: A: PDB Molecule:fanconi anemia-associated protein of 20 kda; PDBTitle: solution structure of the human faap20 ubz Phyre2 PDB 2mur chain A Region: 64 - 79 Aligned: 16 Modelled: 16 Confidence: 21.1% Identity: 31% PDB header:protein binding Chain: A: PDB Molecule:fanconi anemia-associated protein of 20 kda; PDBTitle: solution structure of the human faap20 ubz-ubiquitin complex Phyre2 PDB 6fhs chain H Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 20.8% Identity: 29% PDB header:dna binding protein Chain: H: PDB Molecule:les2; PDBTitle: cryoem structure of ino80core Phyre2 PDB 2m0n chain A Region: 42 - 78 Aligned: 37 Modelled: 37 Confidence: 20.4% Identity: 22% PDB header:unknown function Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: solution structure of a duf3349 annotated protein from mycobacterium2 abscessus, mab_3403c. seattle structural genomics center for3 infectious disease target myaba.17112.a.a2 Phyre2 PDB 3ol4 chain B Region: 48 - 78 Aligned: 31 Modelled: 31 Confidence: 20.3% Identity: 16% PDB header:unknown function Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of a putative uncharacterized protein from2 mycobacterium smegmatis, an ortholog of rv0543c Phyre2 PDB 4y2i chain A Region: 43 - 60 Aligned: 18 Modelled: 18 Confidence: 20.3% Identity: 22% PDB header:metal transport Chain: A: PDB Molecule:putative metal-binding transport protein; PDBTitle: gold ion bound to golb Phyre2 PDB 2db7 chain A domain 1 Region: 30 - 81 Aligned: 37 Modelled: 37 Confidence: 19.9% Identity: 24% Fold: Orange domain-like Superfamily: Orange domain-like Family: Hairy Orange domain Phyre2 PDB 2hh7 chain A Region: 45 - 79 Aligned: 34 Modelled: 35 Confidence: 18.7% Identity: 18% PDB header:unknown function Chain: A: PDB Molecule:hypothetical protein csor; PDBTitle: crystal structure of cu(i) bound csor from mycobacterium tuberculosis. Phyre2 PDB 6cga chain B Region: 49 - 67 Aligned: 16 Modelled: 19 Confidence: 18.4% Identity: 50% PDB header:hydrolase Chain: B: PDB Molecule:polycomb protein asx; PDBTitle: structure of the pr-dub complex Phyre2 PDB 2aj1 chain A Region: 43 - 60 Aligned: 18 Modelled: 18 Confidence: 17.8% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:probable cadmium-transporting atpase; PDBTitle: solution structure of apocada Phyre2 PDB 5cam chain C Region: 61 - 99 Aligned: 39 Modelled: 38 Confidence: 17.7% Identity: 10% PDB header:transcription Chain: C: PDB Molecule:pupr protein; PDBTitle: crystal structure of the cytoplasmic domain of the pseudomonas putida2 anti-sigma factor pupr (semet) Phyre2 PDB 6fml chain H Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 17.3% Identity: 29% PDB header:dna binding protein Chain: H: PDB Molecule:les2; PDBTitle: cryoem structure ino80core nucleosome complex Phyre2 1 c5frhA_ 2 c5wuqD_ 3 c3hugJ_ 4 c3vdoB_ 5 c2z2sD_ 6 d1or7c_ 7 c1or7C_ 8 c6gh5M_ 9 c2muqA_ 10 c2murA_ 11 c6fhsH_ 12 c2m0nA_ 13 c3ol4B_ 14 c4y2iA_ 15 d2db7a1 16 c2hh7A_ 17 c6cgaB_ 18 c2aj1A_ 19 c5camC_ 20 c6fmlH_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99