1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	P	A	R	S	V	P	R	P	R	W	V	A	P	V	R	R	V	G	R	L	A	V	W	D	R	P	E	R	R	S	G	I	P	A	L	D	G	L	R	A	I	A	V	A	L	V	L	A	S	H	G	G	I	P	G	M	G	G	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	I	G	V	D	A	F	F	V	L	S	G	F	L	I	T	S	L	L	L	D	E	L	G	R	T	G	R	I	D	L	S	G	F	W	I	R	R	A	R	R	L	L	P	A	L	V	L	M	V	L	T	V	S	A	A	R	A	L	F
Secondary structure
SS confidence
Disorder																										?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		P	D	Q	A	L	T	G	L	R	S	D	A	I	A	A	F	L	W	T	A	N	W	R	F	V	A	Q	N	T	D	Y	F	T	Q	G	A	P	P	S	P	L	Q	H	T	W	S	L	G	V	E	E	Q	Y	Y	V	V	W	P	L	L
Secondary structure
SS confidence
Disorder																														?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	I	G	A	T	L	L	L	A	A	R	A	R	R	R	C	R	R	A	T	V	G	G	V	R	F	A	A	F	L	I	A	S	L	G	T	M	A	S	A	T	A	A	V	A	F	T	S	A	A	T	R	D	R	I	Y	F	G	T	D
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?	?	?																														?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		T	R	A	Q	A	L	L	I	G	S	A	A	A	A	L	L	V	R	D	W	P	S	L	N	R	G	W	C	L	I	R	T	R	W	G	R	R	I	A	R	L	L	P	F	V	G	L	A	G	L	A	V	T	T	H	V	A	T	G	S
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	G	E	F	R	H	G	L	L	I	V	V	A	G	A	A	V	I	V	V	A	S	V	A	M	E	Q	R	G	A	V	A	R	I	L	A	W	R	P	L	V	W	L	G	T	I	S	Y	G	V	Y	L	W	H	W	P	I	F	L	A
Secondary structure
SS confidence
Disorder		?	?																								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		L	N	G	Q	R	T	G	W	S	G	P	A	L	F	A	A	R	C	A	A	T	V	V	L	A	G	A	S	W	W	L	I	E	Q	P	I	R	R	W	R	P	A	R	V	P	L	L	P	L	A	A	A	T	V	A	S	A	A	A	V
Secondary structure
SS confidence
Disorder																																										?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		T	M	L	V	V	P	V	G	A	G	P	G	L	R	E	I	G	L	P	P	G	V	S	A	V	A	A	V	S	P	S	P	P	E	A	S	Q	P	A	P	G	P	R	D	P	N	R	P	F	T	V	S	V	F	G	D	S	I	G	W
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		T	L	M	H	Y	L	P	P	T	P	G	F	R	F	I	D	H	T	V	I	G	C	S	L	V	R	G	T	P	Y	R	Y	I	G	Q	T	L	E	Q	R	A	E	C	D	G	W	P	A	R	W	S	A	Q	V	N	R	D	Q	P	D
Secondary structure
SS confidence
Disorder																											?	?	?	?	?	?	?		?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		V	A	L	L	I	V	G	R	W	E	T	V	D	R	V	N	E	G	R	W	T	H	I	G	D	P	T	F	D	A	Y	L	N	A	E	L	Q	R	A	L	S	I	V	G	S	T	G	V	R	V	M	V	T	T	V	P	Y	S	R	G
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?	?		?	?																																								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660
Sequence		G	E	K	P	D	G	R	L	Y	P	E	D	Q	P	E	R	V	N	K	W	N	A	M	L	H	N	A	I	S	Q	H	S	N	V	G	M	I	D	L	N	K	K	L	C	P	D	G	V	Y	T	A	K	V	D	G	I	K	V	R	S
Secondary structure
SS confidence
Disorder														?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.	680	.	.	.	.	.
Sequence		D	G	V	H	L	T	Q	E	G	V	K	W	L	I	P	W	L	E	D	S	V	R	V	A	S
Secondary structure
SS confidence
Disorder																								?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 17%) Alpha helix ( 59%) Beta strand ( 5%) TM helix ( 30%) (View membrane topology prediction)

Rank Aligned region

PDB 4iyj chain A Region: 464 - 684 Aligned: 186 Modelled: 202 Confidence: 99.3% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:gdsl-like protein; PDBTitle: crystal structure of a putative acylhydrolase (bacuni_03406) from2 bacteroides uniformis atcc 8492 at 1.37 a resolution Phyre2 PDB 3p94 chain A Region: 447 - 684 Aligned: 201 Modelled: 219 Confidence: 99.3% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:gdsl-like lipase; PDBTitle: crystal structure of a gdsl-like lipase (bdi_0976) from2 parabacteroides distasonis atcc 8503 at 1.93 a resolution Phyre2 PDB 2o14 chain A Region: 464 - 684 Aligned: 191 Modelled: 207 Confidence: 99.3% Identity: 18% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein yxim; PDBTitle: x-ray crystal structure of protein yxim_bacsu from bacillus subtilis.2 northeast structural genomics consortium target sr595 Phyre2 PDB 4rsh chain B Region: 468 - 681 Aligned: 167 Modelled: 180 Confidence: 99.2% Identity: 14% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:lipolytic protein g-d-s-l family; PDBTitle: structure of a putative lipolytic protein of g-d-s-l family from2 desulfitobacterium hafniense dcb-2 Phyre2 PDB 5ufy chain A Region: 466 - 685 Aligned: 165 Modelled: 182 Confidence: 99.2% Identity: 17% PDB header:transferase Chain: A: PDB Molecule:acyltransferase; PDBTitle: structure of streptococcus pneumoniae peptidoglycan o-2 acetyltransferase a (oata) c-terminal catalytic domain Phyre2 PDB 4h08 chain A Region: 463 - 684 Aligned: 183 Modelled: 192 Confidence: 99.2% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:putative hydrolase; PDBTitle: crystal structure of a putative hydrolase (bt3161) from bacteroides2 thetaiotaomicron vpi-5482 at 1.80 a resolution Phyre2 PDB 3mil chain A Region: 469 - 684 Aligned: 184 Modelled: 197 Confidence: 99.1% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:isoamyl acetate-hydrolyzing esterase; PDBTitle: crystal structure of isoamyl acetate-hydrolyzing esterase from2 saccharomyces cerevisiae Phyre2 PDB 4jgg chain B Region: 469 - 684 Aligned: 171 Modelled: 202 Confidence: 99.1% Identity: 19% PDB header:hydrolase Chain: B: PDB Molecule:esterase tesa; PDBTitle: crystal structure of tesa Phyre2 PDB 4k40 chain B Region: 507 - 684 Aligned: 164 Modelled: 178 Confidence: 99.1% Identity: 14% PDB header:hydrolase Chain: B: PDB Molecule:gdsl-like lipase/acylhydrolase family protein; PDBTitle: peptidoglycan o-acetylesterase in action, 0 min Phyre2 PDB 1k7c chain A Region: 470 - 684 Aligned: 191 Modelled: 199 Confidence: 99.1% Identity: 16% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Rhamnogalacturonan acetylesterase Phyre2 PDB 1jrl chain A Region: 468 - 684 Aligned: 172 Modelled: 191 Confidence: 99.1% Identity: 15% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: TAP-like Phyre2 PDB 4s1p chain A Region: 467 - 681 Aligned: 181 Modelled: 214 Confidence: 99.1% Identity: 10% PDB header:hydrolase Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: shel_16390 protein, a putative sgnh hydrolase from slackia2 heliotrinireducens Phyre2 PDB 6njc chain B Region: 463 - 681 Aligned: 182 Modelled: 187 Confidence: 99.0% Identity: 12% PDB header:hydrolase Chain: B: PDB Molecule:sialate o-acetylesterase; PDBTitle: crystal structure of the sialate o-acetylesterase from bacteroides2 vulgatus Phyre2 PDB 4jhl chain A Region: 463 - 681 Aligned: 190 Modelled: 200 Confidence: 99.0% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:acetyl xylan esterase; PDBTitle: crystal structure of of axe2, an acetylxylan esterase from geobacillus2 stearothermophilus Phyre2 PDB 3bzw chain A Region: 461 - 683 Aligned: 203 Modelled: 204 Confidence: 99.0% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:putative lipase; PDBTitle: crystal structure of a putative lipase from bacteroides2 thetaiotaomicron Phyre2 PDB 3bzw chain A domain 1 Region: 461 - 683 Aligned: 203 Modelled: 204 Confidence: 99.0% Identity: 15% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: BT2961-like Phyre2 PDB 1yzf chain A domain 1 Region: 469 - 684 Aligned: 175 Modelled: 188 Confidence: 99.0% Identity: 19% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: TAP-like Phyre2 PDB 2hsj chain A domain 1 Region: 465 - 681 Aligned: 183 Modelled: 198 Confidence: 99.0% Identity: 16% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Acetylhydrolase Phyre2 PDB 5jd3 chain A Region: 466 - 681 Aligned: 187 Modelled: 200 Confidence: 99.0% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:lae5; PDBTitle: crystal structure of lae5, an alpha/beta hydrolase enzyme from the2 metagenome of lake arreo, spain Phyre2 PDB 6iqb chain A Region: 466 - 684 Aligned: 176 Modelled: 207 Confidence: 99.0% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:sgnh-hydrolase family esterase; PDBTitle: esterase aline4 mutant-s13a Phyre2 1 c4iyjA_ 2 c3p94A_ 3 c2o14A_ 4 c4rshB_ 5 c5ufyA_ 6 c4h08A_ 7 c3milA_ 8 c4jggB_ 9 c4k40B_ 10 d1k7ca_ 11 d1jrla_ 12 c4s1pA_ 13 c6njcB_ 14 c4jhlA_ 15 c3bzwA_ 16 d3bzwa1 17 d1yzfa1 18 d2hsja1 19 c5jd3A_ 20 c6iqbA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99