1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	A	S	G	Y	G	G	I	R	V	G	G	P	Y	F	D	D	L	S	K	G	Q	V	F	D	W	A	P	G	V	T	L	S	L	G	L	A	A	A	H	Q	S	I	V	G	N	R	L	R	L	A	L	D	S	D	L	C	A	A	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?																																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	G	M	P	G	P	L	A	H	P	G	L	V	C	D	V	A	I	G	Q	S	T	L	A	T	Q	R	V	K	A	N	L	F	Y	R	G	L	R	F	H	R	F	P	A	V	G	D	T	L	Y	T	R	T	E	V	V	G	L	R	A
Secondary structure
SS confidence
Disorder			?	?																	?	?	?	?	?	?	?	?	?																															?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		N	S	P	K	P	G	R	A	P	T	G	L	A	G	L	R	M	T	T	I	D	R	T	D	R	L	V	L	D	F	Y	R	C	A	M	L	P	A	S	P	D	W	K	P	G	A	V	P	G	D	D	L	S	R	I	G	A	D	A	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?																																?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	P	A	A	D	P	T	A	H	W	D	G	A	V	F	R	K	R	V	P	G	P	H	F	D	A	G	I	A	G	A	V	L	H	S	T	A	D	L	V	S	G	A	P	E	L	A	R	L	T	L	N	I	A	A	T	H	H	D	W
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?																																																?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		R	V	S	G	R	R	L	V	Y	G	G	H	T	I	G	L	A	L	A	Q	A	T	R	L	L	P	N	L	A	T	V	L	D	W	E	S	C	D	H	T	A	P	V	H	E	G	D	T	L	Y	S	E	L	H	I	E	S	A	Q	A
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?	?	?	?																															?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.
Sequence		H	A	D	G	G	V	L	G	L	R	S	L	V	Y	A	V	S	D	S	A	S	E	P	D	R	Q	V	L	D	W	R	F	S	A	L	Q	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?										?	?	?	?	?	?	?	?	?										?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 28%) Alpha helix ( 18%) Beta strand ( 31%) TM helix ( 5%) (View membrane topology prediction)

Rank Aligned region

PDB 2bi0 chain A Region: 8 - 337 Aligned: 330 Modelled: 330 Confidence: 100.0% Identity: 99% PDB header:hypothetical protein Chain: A: PDB Molecule:hypothetical protein rv0216; PDBTitle: rv0216, a conserved hypothetical protein from mycobacterium2 tuberculosis that is essential for bacterial survival3 during infection, has a double hotdogfold Phyre2 PDB 4e3e chain A Region: 10 - 336 Aligned: 319 Modelled: 316 Confidence: 100.0% Identity: 25% PDB header:oxidoreductase Chain: A: PDB Molecule:maoc domain protein dehydratase; PDBTitle: crystal structure of putative maoc domain protein dehydratase from2 chloroflexus aurantiacus j-10-fl Phyre2 PDB 2bi0 chain A domain 1 Region: 8 - 185 Aligned: 178 Modelled: 178 Confidence: 100.0% Identity: 98% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: MaoC-like Phyre2 PDB 2bi0 chain A domain 2 Region: 186 - 337 Aligned: 151 Modelled: 152 Confidence: 100.0% Identity: 95% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: MaoC-like Phyre2 PDB 4v12 chain A Region: 20 - 320 Aligned: 286 Modelled: 301 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:maoc like domain protein; PDBTitle: crystal structure of the msmeg_6754 dehydratase from mycobacterium2 smegmatis Phyre2 PDB 4ffu chain A Region: 10 - 160 Aligned: 146 Modelled: 151 Confidence: 100.0% Identity: 23% PDB header:oxidoreductase Chain: A: PDB Molecule:oxidase; PDBTitle: crystal structure of putative maoc-like (monoamine oxidase-like)2 protein, similar to nodn from sinorhizo bium meliloti 1021 Phyre2 PDB 3exz chain A Region: 12 - 160 Aligned: 143 Modelled: 149 Confidence: 100.0% Identity: 17% PDB header:lyase Chain: A: PDB Molecule:maoc-like dehydratase; PDBTitle: crystal structure of the maoc-like dehydratase from rhodospirillum2 rubrum. northeast structural genomics consortium target rrr103a. Phyre2 PDB 1q6w chain A Region: 11 - 160 Aligned: 143 Modelled: 150 Confidence: 100.0% Identity: 22% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: MaoC-like Phyre2 PDB 5cpg chain A Region: 9 - 172 Aligned: 152 Modelled: 164 Confidence: 100.0% Identity: 19% PDB header:lyase Chain: A: PDB Molecule:(r)-specific enoyl-coa hydratase; PDBTitle: r-hydratase phaj1 from pseudomonas aeruginosa in the unliganded form Phyre2 PDB 1iq6 chain A Region: 16 - 157 Aligned: 131 Modelled: 142 Confidence: 100.0% Identity: 23% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: MaoC-like Phyre2 PDB 2b3n chain A domain 1 Region: 6 - 157 Aligned: 136 Modelled: 148 Confidence: 100.0% Identity: 18% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: MaoC-like Phyre2 PDB 2c2i chain A domain 1 Region: 13 - 150 Aligned: 134 Modelled: 138 Confidence: 100.0% Identity: 14% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: MaoC-like Phyre2 PDB 3ir3 chain B Region: 17 - 161 Aligned: 121 Modelled: 145 Confidence: 99.9% Identity: 12% PDB header:lyase Chain: B: PDB Molecule:3-hydroxyacyl-thioester dehydratase 2; PDBTitle: crystal structure of human 3-hydroxyacyl-thioester dehydratase 22 (htd2) Phyre2 PDB 3khp chain B Region: 20 - 329 Aligned: 257 Modelled: 279 Confidence: 99.9% Identity: 19% PDB header:oxidoreductase Chain: B: PDB Molecule:maoc family protein; PDBTitle: crystal structure of a possible dehydrogenase from mycobacterium2 tuberculosis at 2.3a resolution Phyre2 PDB 4rv2 chain B Region: 13 - 157 Aligned: 132 Modelled: 145 Confidence: 99.9% Identity: 18% PDB header:lyase Chain: B: PDB Molecule:maoc family protein; PDBTitle: crystal structure of (3r)-hydroxyacyl-acp dehydratase hadab hetero-2 dimer from mycobacterium smegmatis Phyre2 PDB 4rlt chain A Region: 15 - 167 Aligned: 146 Modelled: 153 Confidence: 99.9% Identity: 16% PDB header:lyase/lyase inhibitor Chain: A: PDB Molecule:(3r)-hydroxyacyl-acp dehydratase subunit hada; PDBTitle: crystal structure of (3r)-hydroxyacyl-acp dehydratase hadab hetero-2 dimer from mycobacterium tuberculosis complexed with fisetin Phyre2 PDB 4w7b chain D Region: 18 - 155 Aligned: 125 Modelled: 138 Confidence: 99.9% Identity: 15% PDB header:lyase Chain: D: PDB Molecule:hydratase chsh2; PDBTitle: crystal structure of the chsh1:chsh2: complex from mycobacterium2 tuberculosis Phyre2 PDB 5zy8 chain A Region: 20 - 155 Aligned: 124 Modelled: 136 Confidence: 99.9% Identity: 15% PDB header:lyase Chain: A: PDB Molecule:upf0336 protein rv0637; PDBTitle: crystal structure of c terminal truncated hadbc (3r-hydroxyacyl-acp2 dehydratase) complex from mycobacterium tuberculosis Phyre2 PDB 4w7b chain G Region: 27 - 160 Aligned: 129 Modelled: 134 Confidence: 99.9% Identity: 15% PDB header:lyase Chain: G: PDB Molecule:hydratase chsh1; PDBTitle: crystal structure of the chsh1:chsh2: complex from mycobacterium2 tuberculosis Phyre2 PDB 4rv2 chain A Region: 20 - 155 Aligned: 126 Modelled: 136 Confidence: 99.9% Identity: 17% PDB header:lyase Chain: A: PDB Molecule:upf0336 protein msmeg_1340/msmei_1302; PDBTitle: crystal structure of (3r)-hydroxyacyl-acp dehydratase hadab hetero-2 dimer from mycobacterium smegmatis Phyre2 1 c2bi0A_ 2 c4e3eA_ 3 d2bi0a1 4 d2bi0a2 5 c4v12A_ 6 c4ffuA_ 7 c3exzA_ 8 d1q6wa_ 9 c5cpgA_ 10 d1iq6a_ 11 d2b3na1 12 d2c2ia1 13 c3ir3B_ 14 c3khpB_ 15 c4rv2B_ 16 c4rltA_ 17 c4w7bD_ 18 c5zy8A_ 19 c4w7bG_ 20 c4rv2A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186