1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	R	W	F	R	P	G	Y	A	L	V	L	V	L	L	L	A	A	P	L	L	R	P	G	Y	L	L	L	R	D	A	V	S	T	P	R	S	Y	V	S	A	N	A	L	G	L	T	S	A	P	R	A	T	P	Q	D	F	A	V	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																														?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	A	S	H	L	V	D	G	G	V	V	V	K	A	L	L	L	L	G	L	W	L	A	G	W	G	A	A	R	L	V	A	T	A	L	P	A	A	G	A	A	G	Q	F	V	A	I	T	L	A	I	W	N	P	Y	V	A	E	R	L
Secondary structure
SS confidence
Disorder								?																											?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	Q	G	H	W	S	L	L	V	G	Y	G	C	L	P	W	V	A	T	A	M	L	T	M	R	T	T	V	G	A	G	W	F	G	L	F	G	L	A	F	W	V	A	L	A	G	L	T	P	S	G	L	L	L	A	A	T	V	A	V
Secondary structure
SS confidence
Disorder				?																				?		?	?	?		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	C	V	A	M	P	G	A	G	R	P	R	W	Q	C	G	V	A	A	L	G	S	A	L	V	G	A	L	P	W	L	T	A	S	A	L	G	S	S	L	T	S	H	T	A	A	N	Q	L	G	V	T	A	F	A	P	R	A	E	P
Secondary structure
SS confidence
Disorder								?																													?	?	?	?	?	?	?	?	?	?	?	?										?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		G	L	G	T	L	G	S	L	A	S	L	G	G	I	W	N	G	E	A	V	P	S	S	R	T	T	L	F	A	V	A	S	A	V	V	L	L	A	M	V	A	I	G	L	P	T	V	A	R	R	P	V	A	V	P	L	L	T	L	A
Secondary structure
SS confidence
Disorder		?	?	?	?					?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	V	S	V	M	V	P	A	V	L	A	T	G	P	G	L	H	A	L	R	V	V	V	D	A	A	P	G	L	G	V	L	R	D	G	Q	K	W	V	A	L	A	V	P	G	Y	T	L	S	G	A	G	T	V	L	T	L	R	R	W
Secondary structure
SS confidence
Disorder																																																											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		L	R	P	A	T	A	A	V	V	C	C	L	A	L	V	L	T	L	P	D	L	A	W	G	V	W	G	K	V	A	P	V	H	Y	P	S	G	W	A	A	V	A	A	A	I	N	A	D	P	R	T	V	A	V	L	P	A	G	T	M
Secondary structure
SS confidence
Disorder					?	?															?			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		R	R	F	S	W	S	G	S	A	P	V	L	D	P	L	P	R	W	V	R	A	D	V	L	T	T	G	D	L	V	I	S	G	V	T	V	P	G	E	D	A	H	A	R	A	V	Q	E	L	L	L	T	G	P	H	P	S	T	L	A
Secondary structure
SS confidence
Disorder																							?			?	?	?			?	?	?		?	?	?	?												?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		A	A	G	V	G	W	L	V	V	E	S	D	S	A	G	D	M	G	A	A	A	R	T	L	G	R	L	A	A	A	H	R	D	D	E	L	A	L	Y	R	V	G	G	Q	T	S	G	A	S	S	A	R	L	K	A	T	M	L	A	H
Secondary structure
SS confidence
Disorder															?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?											?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.
Sequence		W	A	W	L	S	M	L	L	V	G	G	A	G	A	A	G	Y	W	V	R	R	H	L	H	H	C	E	D	T	P	A	S	R	A	Q	D
Secondary structure
SS confidence
Disorder																								?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 20%) Alpha helix ( 57%) Beta strand ( 5%) TM helix ( 41%) (View membrane topology prediction)

Rank Aligned region

PDB 3waj chain A Region: 55 - 525 Aligned: 463 Modelled: 471 Confidence: 99.1% Identity: 10% PDB header:transferase Chain: A: PDB Molecule:transmembrane oligosaccharyl transferase; PDBTitle: crystal structure of the archaeoglobus fulgidus2 oligosaccharyltransferase (o29867_arcfu) complex with zn and sulfate Phyre2 PDB 3rce chain A Region: 45 - 417 Aligned: 345 Modelled: 348 Confidence: 98.6% Identity: 11% PDB header:transferase/peptide Chain: A: PDB Molecule:oligosaccharide transferase to n-glycosylate proteins; PDBTitle: bacterial oligosaccharyltransferase pglb Phyre2 PDB 6ezn chain F Region: 81 - 514 Aligned: 383 Modelled: 409 Confidence: 98.4% Identity: 12% PDB header:membrane protein Chain: F: PDB Molecule:dolichyl-diphosphooligosaccharide--protein PDBTitle: cryo-em structure of the yeast oligosaccharyltransferase (ost) complex Phyre2 PDB 5f15 chain A Region: 55 - 492 Aligned: 421 Modelled: 438 Confidence: 97.6% Identity: 13% PDB header:transferase Chain: A: PDB Molecule:4-amino-4-deoxy-l-arabinose (l-ara4n) transferase; PDBTitle: crystal structure of arnt from cupriavidus metallidurans bound to2 undecaprenyl phosphate Phyre2 PDB 3vu0 chain B Region: 393 - 496 Aligned: 92 Modelled: 102 Confidence: 33.6% Identity: 12% PDB header:transferase Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of the c-terminal globular domain of2 oligosaccharyltransferase (afaglb-s2, af_0040, o30195_arcfu) from3 archaeoglobus fulgidus Phyre2 PDB 3vgp chain A Region: 396 - 490 Aligned: 74 Modelled: 74 Confidence: 25.3% Identity: 14% PDB header:transferase Chain: A: PDB Molecule:transmembrane oligosaccharyl transferase, putative; PDBTitle: crystal structure of the c-terminal globular domain of2 oligosaccharyltransferase (af_0329) from archaeoglobus fulgidus Phyre2 PDB 2l9u chain A Region: 537 - 565 Aligned: 26 Modelled: 29 Confidence: 25.3% Identity: 42% PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-3; PDBTitle: spatial structure of dimeric erbb3 transmembrane domain Phyre2 PDB 3wai chain A Region: 393 - 490 Aligned: 94 Modelled: 98 Confidence: 21.9% Identity: 10% PDB header:transferase, transport protein Chain: A: PDB Molecule:maltose-binding periplasmic protein, transmembrane PDBTitle: crystal structure of the c-terminal globular domain of2 oligosaccharyltransferase (afaglb-l, o29867_arcfu) from archaeoglobus3 fulgidus as a mbp fusion Phyre2 PDB 4dl0 chain I Region: 486 - 521 Aligned: 36 Modelled: 36 Confidence: 21.1% Identity: 28% PDB header:hydrolase Chain: I: PDB Molecule:v-type proton atpase subunit c; PDBTitle: crystal structure of the heterotrimeric egchead peripheral stalk2 complex of the yeast vacuolar atpase Phyre2 PDB 1qki chain A domain 1 Region: 479 - 522 Aligned: 42 Modelled: 44 Confidence: 19.9% Identity: 21% Fold: NAD(P)-binding Rossmann-fold domains Superfamily: NAD(P)-binding Rossmann-fold domains Family: Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain Phyre2 PDB 6p25 chain A Region: 70 - 217 Aligned: 145 Modelled: 148 Confidence: 18.7% Identity: 5% PDB header:transferase Chain: A: PDB Molecule:dolichyl-phosphate-mannose--protein mannosyltransferase 1; PDBTitle: structure of s. cerevisiae protein o-mannosyltransferase pmt1-pmt22 complex bound to the sugar donor and a peptide acceptor Phyre2 PDB 1h9a chain A domain 1 Region: 487 - 522 Aligned: 34 Modelled: 36 Confidence: 18.1% Identity: 26% Fold: NAD(P)-binding Rossmann-fold domains Superfamily: NAD(P)-binding Rossmann-fold domains Family: Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain Phyre2 PDB 5vyl chain A Region: 16 - 34 Aligned: 19 Modelled: 19 Confidence: 17.9% Identity: 37% PDB header:viral protein Chain: A: PDB Molecule:inner tegument protein; PDBTitle: crystal structure of n-terminal half of herpes simplex virus type 12 ul37 protein Phyre2 PDB 4k70 chain B Region: 19 - 34 Aligned: 16 Modelled: 16 Confidence: 17.1% Identity: 25% PDB header:viral protein Chain: B: PDB Molecule:ul37; PDBTitle: crystal structure of n-terminal half of pseudorabiesvirus ul37 protein Phyre2 PDB 1a77 chain A domain 2 Region: 477 - 519 Aligned: 43 Modelled: 43 Confidence: 13.1% Identity: 19% Fold: PIN domain-like Superfamily: PIN domain-like Family: 5' to 3' exonuclease catalytic domain Phyre2 PDB 3gxg chain A Region: 468 - 488 Aligned: 21 Modelled: 21 Confidence: 12.0% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:putative phosphatase (duf442); PDBTitle: crystal structure of putative phosphatase (duf442) (yp_001181608.1)2 from shewanella putrefaciens cn-32 at 1.60 a resolution Phyre2 PDB 4j2l chain C Region: 404 - 423 Aligned: 20 Modelled: 20 Confidence: 12.0% Identity: 40% PDB header:transcription regulator Chain: C: PDB Molecule:protein capicua homolog; PDBTitle: crystal structure of axh domain complexed with capicua Phyre2 PDB 4j2l chain D Region: 404 - 423 Aligned: 20 Modelled: 20 Confidence: 12.0% Identity: 40% PDB header:transcription regulator Chain: D: PDB Molecule:protein capicua homolog; PDBTitle: crystal structure of axh domain complexed with capicua Phyre2 PDB 6p2r chain B Region: 53 - 217 Aligned: 162 Modelled: 163 Confidence: 11.7% Identity: 7% PDB header:transferase Chain: B: PDB Molecule:dolichyl-phosphate-mannose--protein mannosyltransferase 2; PDBTitle: structure of s. cerevisiae protein o-mannosyltransferase pmt1-pmt22 complex bound to the sugar donor Phyre2 PDB 5kzo chain A Region: 544 - 567 Aligned: 24 Modelled: 24 Confidence: 11.6% Identity: 17% PDB header:transcription Chain: A: PDB Molecule:neurogenic locus notch homolog protein 1; PDBTitle: notch1 transmembrane and associated juxtamembrane segment Phyre2 1 c3wajA_ 2 c3rceA_ 3 c6eznF_ 4 c5f15A_ 5 c3vu0B_ 6 c3vgpA_ 7 c2l9uA_ 8 c3waiA_ 9 c4dl0I_ 10 d1qkia1 11 c6p25A_ 12 d1h9aa1 13 c5vylA_ 14 c4k70B_ 15 d1a77a2 16 c3gxgA_ 17 c4j2lC_ 18 c4j2lD_ 19 c6p2rB_ 20 c5kzoA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99