1

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10

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20

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30

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40

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50

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Sequence

M

N

R

I

V

A

P

A

A

A

S

V

V

V

G

L

L

L

G

A

A

A

I

F

G

V

T

L

M

V

Q

Q

D

K

K

P

P

L

P

G

G

D

P

S

S

S

V

L

N

R

V

E

Y

G

N

R

S

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 49%) Alpha helix ( 68%) Beta strand ( 4%) TM helix ( 28%) (View membrane topology prediction)

Rank Aligned region

PDB 2k1l chain A Region: 12 - 31 Aligned: 20 Modelled: 20 Confidence: 30.9% Identity: 45% PDB header:signaling protein Chain: A: PDB Molecule:ephrin type-a receptor 1; PDBTitle: nmr structures of dimeric transmembrane domain of the2 receptor tyrosine kinase epha1 in lipid bicelles at ph 6.3 Phyre2 PDB 2k1k chain B Region: 12 - 31 Aligned: 20 Modelled: 20 Confidence: 30.9% Identity: 45% PDB header:signaling protein Chain: B: PDB Molecule:ephrin type-a receptor 1; PDBTitle: nmr structures of dimeric transmembrane domain of the2 receptor tyrosine kinase epha1 in lipid bicelles at ph 4.3 Phyre2 PDB 2k1k chain A Region: 12 - 31 Aligned: 20 Modelled: 20 Confidence: 30.9% Identity: 45% PDB header:signaling protein Chain: A: PDB Molecule:ephrin type-a receptor 1; PDBTitle: nmr structures of dimeric transmembrane domain of the2 receptor tyrosine kinase epha1 in lipid bicelles at ph 4.3 Phyre2 PDB 2k1l chain B Region: 12 - 31 Aligned: 20 Modelled: 20 Confidence: 30.9% Identity: 45% PDB header:signaling protein Chain: B: PDB Molecule:ephrin type-a receptor 1; PDBTitle: nmr structures of dimeric transmembrane domain of the2 receptor tyrosine kinase epha1 in lipid bicelles at ph 6.3 Phyre2 PDB 1q90 chain R Region: 1 - 24 Aligned: 24 Modelled: 24 Confidence: 17.0% Identity: 42% Fold: Single transmembrane helix Superfamily: ISP transmembrane anchor Family: ISP transmembrane anchor Phyre2 PDB 1q90 chain R Region: 1 - 24 Aligned: 24 Modelled: 24 Confidence: 17.0% Identity: 42% PDB header:photosynthesis Chain: R: PDB Molecule:cytochrome b6-f complex iron-sulfur subunit; PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii Phyre2 PDB 4cdi chain C Region: 4 - 36 Aligned: 33 Modelled: 33 Confidence: 16.9% Identity: 24% PDB header:membrane protein Chain: C: PDB Molecule:predicted protein; PDBTitle: crystal structure of acrb-acrz complex Phyre2 PDB 2jwa chain A Region: 11 - 34 Aligned: 24 Modelled: 24 Confidence: 15.3% Identity: 42% PDB header:transferase Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-2; PDBTitle: erbb2 transmembrane segment dimer spatial structure Phyre2 PDB 2ks1 chain A Region: 11 - 34 Aligned: 24 Modelled: 24 Confidence: 15.3% Identity: 42% PDB header:transferase Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-2; PDBTitle: heterodimeric association of transmembrane domains of erbb1 and erbb22 receptors enabling kinase activation Phyre2 PDB 4etr chain A Region: 40 - 54 Aligned: 15 Modelled: 15 Confidence: 14.8% Identity: 53% PDB header:unknown function Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: x-ray structure of pa2169 from pseudomonas aeruginosa Phyre2 PDB 2lp1 chain A Region: 11 - 26 Aligned: 16 Modelled: 16 Confidence: 11.2% Identity: 31% PDB header:membrane protein Chain: A: PDB Molecule:c99; PDBTitle: the solution nmr structure of the transmembrane c-terminal domain of2 the amyloid precursor protein (c99) Phyre2 PDB 4xyp chain A Region: 42 - 54 Aligned: 13 Modelled: 13 Confidence: 10.4% Identity: 46% PDB header:viral protein Chain: A: PDB Molecule:fusion protein; PDBTitle: crystal structure of a piscine viral fusion protein Phyre2 PDB 1r7m chain A domain 1 Region: 5 - 21 Aligned: 17 Modelled: 17 Confidence: 9.0% Identity: 29% Fold: Homing endonuclease-like Superfamily: Homing endonucleases Family: Group I mobile intron endonuclease Phyre2 PDB 2k9p chain A Region: 12 - 38 Aligned: 27 Modelled: 27 Confidence: 8.7% Identity: 26% PDB header:membrane protein Chain: A: PDB Molecule:pheromone alpha factor receptor; PDBTitle: structure of tm1_tm2 in lppg micelles Phyre2 PDB 1r7m chain A Region: 5 - 21 Aligned: 17 Modelled: 17 Confidence: 7.7% Identity: 29% PDB header:hydrolase/dna Chain: A: PDB Molecule:intron-encoded endonuclease i-scei; PDBTitle: the homing endonuclease i-scei bound to its dna recognition2 region Phyre2 PDB 1jb0 chain K Region: 10 - 25 Aligned: 16 Modelled: 16 Confidence: 7.2% Identity: 38% PDB header:photosynthesis Chain: K: PDB Molecule:photosystem 1 reaction centre subunit x; PDBTitle: crystal structure of photosystem i: a photosynthetic reaction center2 and core antenna system from cyanobacteria Phyre2 PDB 1jb0 chain K Region: 10 - 25 Aligned: 16 Modelled: 16 Confidence: 7.2% Identity: 38% Fold: Photosystem I reaction center subunit X, PsaK Superfamily: Photosystem I reaction center subunit X, PsaK Family: Photosystem I reaction center subunit X, PsaK Phyre2 PDB 3q7c chain A Region: 6 - 17 Aligned: 12 Modelled: 12 Confidence: 6.6% Identity: 33% PDB header:hydrolase Chain: A: PDB Molecule:nucleoprotein; PDBTitle: exonuclease domain of lassa virus nucleoprotein bound to manganese Phyre2 PDB 2k8p chain A Region: 35 - 57 Aligned: 23 Modelled: 23 Confidence: 6.5% Identity: 30% PDB header:signaling protein Chain: A: PDB Molecule:sclerostin; PDBTitle: characterisation of the structural features and2 interactions of sclerostin: molecular insight into a key3 regulator of wnt-mediated bone formation Phyre2 PDB 1h41 chain A domain 2 Region: 21 - 40 Aligned: 20 Modelled: 20 Confidence: 6.2% Identity: 15% Fold: Zincin-like Superfamily: beta-N-acetylhexosaminidase-like domain Family: alpha-D-glucuronidase, N-terminal domain Phyre2 1 c2k1lA_ 2 c2k1kB_ 3 c2k1kA_ 4 c2k1lB_ 5 d1q90r_ 6 c1q90R_ 7 c4cdiC_ 8 c2jwaA_ 9 c2ks1A_ 10 c4etrA_ 11 c2lp1A_ 12 c4xypA_ 13 d1r7ma1 14 c2k9pA_ 15 c1r7mA_ 16 c1jb0K_ 17 d1jb0k_ 18 c3q7cA_ 19 c2k8pA_ 20 d1h41a2 21 22 23