1

.

.

.

.

.

.

.

.

10

.

.

.

.

.

.

.

.

.

20

.

.

.

.

.

.

.

.

.

30

.

.

.

.

.

.

.

.

.

40

.

.

.

.

.

.

.

.

.

50

.

.

.

.

.

.

.

.

.

60

Sequence

M

A

A

P

I

W

M

A

S

P

P

E

V

H

S

A

L

L

S

N

G

P

G

P

G

S

L

V

A

A

A

T

A

W

S

Q

L

S

A

E

Y

A

S

T

A

A

E

L

S

G

L

L

G

A

V

P

G

W

A

W

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

70

.

.

.

.

.

.

.

.

.

80

.

.

.

.

.

.

.

.

.

90

.

.

.

.

.

.

.

.

.

100

.

.

.

.

.

.

.

.

.

110

.

.

.

.

.

.

.

.

.

120

Sequence

Q

G

P

S

A

E

W

Y

V

A

A

H

L

P

Y

V

A

W

L

T

Q

A

S

A

D

A

A

G

A

A

A

Q

H

E

A

A

A

A

A

Y

T

T

A

L

A

A

M

P

T

L

A

E

L

A

A

N

H

V

I

H

Secondary structure

SS confidence

Disorder

Disorder confidence

.

.

.

.

.

.

.

.

.

130

.

.

.

.

.

.

.

.

.

140

.

.

.

.

.

.

.

.

.

150

.

.

.

.

.

.

.

.

.

160

.

.

.

.

.

.

.

.

.

170

.

.

.

.

.

.

.

.

.

180

Sequence

T

V

L

V

A

T

N

F

F

G

I

N

T

I

P

I

T

L

N

E

A

D

Y

V

R

M

W

L

Q

A

A

A

V

M

G

L

Y

Q

A

A

S

G

A

A

L

A

S

A

P

R

T

V

P

A

P

T

V

M

N

P

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

190

.

.

.

.

.

.

.

.

.

200

.

.

.

.

.

.

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.

.

210

.

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.

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220

.

.

.

.

.

.

.

.

.

230

.

.

.

.

.

.

.

.

.

240

Sequence

G

G

G

A

A

S

T

V

G

A

V

N

P

W

Q

W

L

L

A

L

L

Q

Q

L

W

N

A

Y

T

G

F

Y

G

W

M

L

Q

L

I

W

Q

F

L

Q

D

P

I

G

N

S

I

K

I

I

I

A

F

L

T

N

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

250

.

.

.

.

.

.

.

.

.

260

.

.

.

.

.

.

.

.

.

270

.

.

.

.

.

.

.

.

.

280

.

.

.

.

.

.

.

.

.

290

.

.

.

.

.

.

.

.

.

300

Sequence

P

I

Q

A

L

I

T

Y

G

P

L

L

F

A

L

G

Y

Q

I

F

F

N

L

V

G

W

P

T

W

G

M

I

L

S

S

P

F

L

L

P

A

G

L

G

L

G

L

A

A

I

A

F

L

P

I

V

L

A

P

A

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

310

.

.

.

.

.

.

.

.

.

320

.

.

.

.

.

.

.

.

.

330

.

.

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.

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.

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340

.

.

.

.

.

.

.

.

.

350

.

.

.

.

.

.

.

.

.

360

Sequence

V

I

P

P

A

S

T

P

L

A

A

A

A

V

A

A

G

S

V

W

P

A

V

S

M

A

V

T

G

A

G

T

A

G

A

A

T

P

A

A

G

A

A

P

S

A

G

A

A

P

A

P

A

A

P

A

T

A

S

F

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

370

.

.

.

.

.

.

.

.

.

380

.

.

.

.

.

.

.

.

.

390

.

.

.

.

.

.

.

.

.

400

.

.

.

.

.

.

.

.

.

410

.

.

.

.

.

.

.

.

.

420

Sequence

A

Y

A

V

G

G

S

G

D

W

G

P

S

L

G

P

T

V

G

G

R

G

G

I

K

A

P

A

A

T

V

P

A

A

A

A

A

A

A

T

R

G

Q

S

R

A

R

R

R

R

R

S

E

L

R

D

Y

G

D

E

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

430

.

.

.

.

.

.

.

.

.

440

.

.

.

.

.

.

.

.

.

450

.

.

.

.

.

.

.

.

.

460

.

.

.

.

.

.

.

.

.

470

.

.

.

.

.

.

.

.

.

480

Sequence

F

L

D

M

D

S

D

S

G

F

G

P

S

T

G

D

H

G

A

Q

A

S

E

R

G

A

G

T

L

G

F

A

G

T

A

T

K

E

R

R

V

R

A

V

G

L

T

A

L

A

G

D

E

F

G

N

G

P

R

M

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

490

.

.

.

.

.

.

.

.

.

500

.

.

.

.

.

.

.

.

.

510

.

.

.

Sequence

P

M

V

P

G

T

W

E

Q

G

S

N

E

P

E

A

P

D

G

S

G

R

G

G

G

D

G

L

P

H

D

S

K

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 51%) Alpha helix ( 49%) Beta strand ( 1%) TM helix ( 9%) (View membrane topology prediction)

Rank Aligned region

PDB 5xfs chain B Region: 6 - 176 Aligned: 171 Modelled: 171 Confidence: 100.0% Identity: 43% PDB header:protein transport Chain: B: PDB Molecule:ppe family protein ppe15; PDBTitle: crystal structure of pe8-ppe15 in complex with espg5 from m.2 tuberculosis Phyre2 PDB 2g38 chain B domain 1 Region: 6 - 177 Aligned: 171 Modelled: 172 Confidence: 100.0% Identity: 30% Fold: Ferritin-like Superfamily: PE/PPE dimer-like Family: PPE Phyre2 PDB 2g38 chain B Region: 6 - 177 Aligned: 171 Modelled: 172 Confidence: 100.0% Identity: 30% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:ppe family protein; PDBTitle: a pe/ppe protein complex from mycobacterium tuberculosis Phyre2 PDB 4xy3 chain A Region: 3 - 174 Aligned: 155 Modelled: 172 Confidence: 100.0% Identity: 19% PDB header:protein transport Chain: A: PDB Molecule:esx-1 secretion-associated protein espb; PDBTitle: structure of esx-1 secreted protein espb Phyre2 PDB 4wj2 chain A Region: 17 - 113 Aligned: 92 Modelled: 97 Confidence: 98.1% Identity: 18% PDB header:unknown function Chain: A: PDB Molecule:antigen mtb48; PDBTitle: mycobacterial protein Phyre2 PDB 2vs0 chain B Region: 24 - 103 Aligned: 79 Modelled: 80 Confidence: 97.7% Identity: 14% PDB header:cell invasion Chain: B: PDB Molecule:virulence factor esxa; PDBTitle: structural analysis of homodimeric staphylococcal aureus2 virulence factor esxa Phyre2 PDB 4iog chain D Region: 24 - 102 Aligned: 78 Modelled: 79 Confidence: 97.5% Identity: 15% PDB header:unknown function Chain: D: PDB Molecule:secreted protein esxb; PDBTitle: the crystal structure of a secreted protein esxb (wild-type, in p212 space group) from bacillus anthracis str. sterne Phyre2 PDB 3gvm chain A Region: 24 - 103 Aligned: 79 Modelled: 80 Confidence: 97.5% Identity: 14% PDB header:viral protein Chain: A: PDB Molecule:putative uncharacterized protein sag1039; PDBTitle: structure of the homodimeric wxg-100 family protein from streptococcus2 agalactiae Phyre2 PDB 3zbh chain C Region: 24 - 103 Aligned: 79 Modelled: 80 Confidence: 97.1% Identity: 16% PDB header:unknown function Chain: C: PDB Molecule:esxa; PDBTitle: geobacillus thermodenitrificans esxa crystal form i Phyre2 PDB 1wa8 chain A domain 1 Region: 24 - 101 Aligned: 77 Modelled: 78 Confidence: 96.6% Identity: 14% Fold: Ferritin-like Superfamily: EsxAB dimer-like Family: ESAT-6 like Phyre2 PDB 4lws chain B Region: 25 - 100 Aligned: 75 Modelled: 76 Confidence: 94.0% Identity: 19% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: esxa : esxb (semet) hetero-dimer from thermomonospora curvata Phyre2 PDB 1wa8 chain B domain 1 Region: 25 - 103 Aligned: 78 Modelled: 79 Confidence: 93.6% Identity: 22% Fold: Ferritin-like Superfamily: EsxAB dimer-like Family: ESAT-6 like Phyre2 PDB 4lws chain A Region: 24 - 103 Aligned: 79 Modelled: 77 Confidence: 92.8% Identity: 11% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: esxa : esxb (semet) hetero-dimer from thermomonospora curvata Phyre2 PDB 4i0x chain A Region: 27 - 100 Aligned: 73 Modelled: 74 Confidence: 91.7% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:esat-6-like protein mab_3112; PDBTitle: crystal structure of the mycobacterum abscessus esxef (mab_3112-2 mab_3113) complex Phyre2 PDB 2kg7 chain B Region: 34 - 103 Aligned: 69 Modelled: 70 Confidence: 86.2% Identity: 20% PDB header:unknown function Chain: B: PDB Molecule:esat-6-like protein esxh; PDBTitle: structure and features of the complex formed by the tuberculosis2 virulence factors rv0287 and rv0288 Phyre2 PDB 4i0x chain J Region: 46 - 100 Aligned: 50 Modelled: 55 Confidence: 68.1% Identity: 28% PDB header:structural genomics, unknown function Chain: J: PDB Molecule:esat-6-like protein mab_3113; PDBTitle: crystal structure of the mycobacterum abscessus esxef (mab_3112-2 mab_3113) complex Phyre2 PDB 1ui5 chain A domain 2 Region: 74 - 147 Aligned: 54 Modelled: 72 Confidence: 38.2% Identity: 19% Fold: Tetracyclin repressor-like, C-terminal domain Superfamily: Tetracyclin repressor-like, C-terminal domain Family: Tetracyclin repressor-like, C-terminal domain Phyre2 PDB 1zee chain A domain 1 Region: 125 - 154 Aligned: 30 Modelled: 29 Confidence: 24.1% Identity: 33% Fold: Indolic compounds 2,3-dioxygenase-like Superfamily: Indolic compounds 2,3-dioxygenase-like Family: Indoleamine 2,3-dioxygenase-like Phyre2 PDB 1dlp chain A domain 1 Region: 5 - 21 Aligned: 17 Modelled: 17 Confidence: 14.9% Identity: 18% Fold: beta-Prism II Superfamily: alpha-D-mannose-specific plant lectins Family: alpha-D-mannose-specific plant lectins Phyre2 PDB 3zfs chain A Region: 90 - 156 Aligned: 67 Modelled: 67 Confidence: 13.0% Identity: 28% PDB header:oxidoreductase Chain: A: PDB Molecule:f420-reducing hydrogenase, subunit alpha; PDBTitle: cryo-em structure of the f420-reducing nife-hydrogenase from a2 methanogenic archaeon with bound substrate Phyre2 1 c5xfsB_ 2 d2g38b1 3 c2g38B_ 4 c4xy3A_ 5 c4wj2A_ 6 c2vs0B_ 7 c4iogD_ 8 c3gvmA_ 9 c3zbhC_ 10 d1wa8a1 11 c4lwsB_ 12 d1wa8b1 13 c4lwsA_ 14 c4i0xA_ 15 c2kg7B_ 16 c4i0xJ_ 17 d1ui5a2 18 d1zeea1 19 d1dlpa1 20 c3zfsA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60