1

.

.

.

.

.

.

.

.

10

.

.

.

.

.

.

.

.

.

20

.

.

.

.

.

.

.

.

.

30

.

.

.

.

.

.

.

.

.

40

.

.

.

.

.

.

.

.

.

50

.

.

.

.

.

.

.

.

.

60

Sequence

M

S

V

V

G

G

T

V

R

T

V

G

R

T

V

S

G

A

A

T

A

T

T

A

A

A

G

A

V

G

G

A

A

V

S

G

I

V

G

G

V

T

G

A

A

K

G

I

Q

K

G

L

S

S

G

S

K

S

T

A

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

70

.

.

.

.

.

.

.

.

.

80

.

.

.

.

.

.

.

.

.

90

.

.

.

.

.

.

.

.

.

100

.

.

.

.

.

.

.

.

.

110

.

.

.

.

.

.

.

.

.

120

Sequence

A

A

A

L

A

I

G

A

I

G

V

A

G

L

V

D

W

P

I

L

L

A

V

G

G

G

A

L

L

L

R

K

L

N

R

T

P

E

V

A

A

P

P

V

K

A

K

L

A

P

V

P

D

K

P

A

A

A

K

E

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

130

.

.

.

.

.

.

.

.

.

140

.

.

.

.

.

.

.

Sequence

A

P

A

K

A

S

K

T

T

A

R

K

T

S

G

R

R

A

G

T

A

E

L

R

S

T

N

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 54%) Alpha helix ( 75%) Beta strand ( 0%)

Rank Aligned region

PDB 1be3 chain K Region: 67 - 75 Aligned: 9 Modelled: 9 Confidence: 7.6% Identity: 67% Fold: Single transmembrane helix Superfamily: Subunit XI (6.4 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase) Family: Subunit XI (6.4 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase) Phyre2 PDB 5nmi chain X Region: 67 - 75 Aligned: 9 Modelled: 9 Confidence: 7.6% Identity: 67% PDB header:membrane protein Chain: X: PDB Molecule:arg-asn-trp-val-pro-thr-ala-gln-leu-trp-gly-ala-val-gly- PDBTitle: cytochrome bc1 bound to the inhibitor mjm170 Phyre2 PDB 5nmi chain K Region: 67 - 75 Aligned: 9 Modelled: 9 Confidence: 7.6% Identity: 67% PDB header:membrane protein Chain: K: PDB Molecule:arg-asn-trp-val-pro-thr-ala-gln-leu-trp-gly-ala-val-gly- PDBTitle: cytochrome bc1 bound to the inhibitor mjm170 Phyre2 PDB 2x43 chain S Region: 41 - 58 Aligned: 18 Modelled: 18 Confidence: 7.0% Identity: 39% PDB header:membrane protein Chain: S: PDB Molecule:sherp; PDBTitle: structural basis of molecular recognition by sherp at membrane2 surfaces Phyre2 PDB 2lj2 chain A Region: 59 - 77 Aligned: 19 Modelled: 19 Confidence: 6.9% Identity: 21% PDB header:membrane protein Chain: A: PDB Molecule:merf; PDBTitle: integral membrane core domain of the mercury transporter merf in lipid2 bilayer membranes Phyre2 PDB 1ifl chain A Region: 77 - 94 Aligned: 18 Modelled: 18 Confidence: 6.7% Identity: 28% PDB header:virus Chain: A: PDB Molecule:inovirus; PDBTitle: molecular models and structural comparisons of native and2 mutant class i filamentous bacteriophages ff (fd, f1, m13),3 if1 and ike Phyre2 PDB 2m67 chain A Region: 59 - 77 Aligned: 19 Modelled: 19 Confidence: 6.1% Identity: 21% PDB header:transport protein Chain: A: PDB Molecule:merf; PDBTitle: full-length mercury transporter protein merf in lipid bilayer2 membranes Phyre2 PDB 1waz chain A Region: 59 - 77 Aligned: 19 Modelled: 19 Confidence: 6.1% Identity: 21% PDB header:transport protein Chain: A: PDB Molecule:merf; PDBTitle: nmr structure determination of the bacterial mercury transporter,2 merf, in micelles Phyre2 PDB 4b0t chain B Region: 69 - 91 Aligned: 23 Modelled: 23 Confidence: 5.9% Identity: 35% PDB header:ligase Chain: B: PDB Molecule:pup--protein ligase; PDBTitle: structure of the pup ligase pafa of the prokaryotic2 ubiquitin-like modification pathway in complex with adp Phyre2 PDB 4bjr chain A Region: 69 - 91 Aligned: 23 Modelled: 23 Confidence: 5.8% Identity: 35% PDB header:ligase Chain: A: PDB Molecule:pup--protein ligase, prokaryotic ubiquitin-like protein PDBTitle: crystal structure of the complex between prokaryotic2 ubiquitin-like protein pup and its ligase pafa Phyre2 PDB 2w4z chain B Region: 84 - 96 Aligned: 13 Modelled: 13 Confidence: 5.6% Identity: 54% PDB header:virus Chain: B: PDB Molecule:caulobacter bacteriophage 5; PDBTitle: caulobacter bacteriophage 5 Phyre2 1 d1be3k_ 2 c5nmiX_ 3 c5nmiK_ 4 c2x43S_ 5 c2lj2A_ 6 c1iflA_ 7 c2m67A_ 8 c1wazA_ 9 c4b0tB_ 10 c4bjrA_ 11 c2w4zB_