1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		L	P	A	R	R	S	A	A	R	I	D	F	A	G	S	P	R	P	T	L	G	V	E	W	E	F	A	L	V	D	S	Q	T	R	D	L	S	N	E	A	T	A	V	I	A	E	I	G	E	N	P	R	V	H	K	E	L	L	R	N
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?		?																		?		?	?	?	?										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	V	E	I	V	S	G	I	C	E	C	T	A	E	A	M	Q	D	L	R	D	T	L	G	P	A	R	Q	I	V	R	D	R	G	M	E	L	F	C	A	G	T	H	P	F	A	R	W	S	A	Q	K	L	T	D	A	P	R	Y	A
Secondary structure
SS confidence
Disorder																																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		E	L	I	K	R	T	Q	W	W	G	R	Q	M	L	I	W	G	V	H	V	H	V	G	I	R	S	A	H	K	V	M	P	I	M	T	S	L	L	N	Y	Y	P	H	L	L	A	L	S	A	S	S	P	W	W	G	G	E	D	T	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Y	A	S	N	R	A	M	M	F	Q	Q	L	P	T	A	G	L	P	F	H	F	Q	R	W	A	E	F	E	G	F	V	Y	D	Q	K	K	T	G	I	I	D	H	M	D	E	I	R	W	D	I	R	P	S	P	H	L	G	T	L	E
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		V	R	I	C	D	G	V	S	N	L	R	E	L	G	A	L	V	A	L	T	H	C	L	I	V	D	L	D	R	R	L	D	A	G	E	T	L	P	T	M	P	P	W	H	V	Q	E	N	K	W	R	A	A	R	Y	G	L	D	A	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		I	I	L	D	A	D	S	N	E	R	L	V	T	D	D	L	A	D	V	L	T	R	L	E	P	V	A	K	S	L	N	C	A	D	E	L	A	A	V	S	D	I	Y	R	D	G	A	S	Y	Q	R	Q	L	R	V	A	Q	Q	H	D
Secondary structure
SS confidence
Disorder																																																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.
Sequence		G	D	L	R	A	V	V	D	A	L	V	A	E	L	V	I
Secondary structure
SS confidence
Disorder																?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 7%) Alpha helix ( 48%) Beta strand ( 12%)

Rank Aligned region

PDB 1r8g chain A Region: 11 - 372 Aligned: 344 Modelled: 346 Confidence: 100.0% Identity: 28% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase family 2 (GCS2) Phyre2 PDB 1tt4 chain A Region: 11 - 372 Aligned: 348 Modelled: 361 Confidence: 100.0% Identity: 27% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase family 2 (GCS2) Phyre2 PDB 1tt4 chain B Region: 11 - 372 Aligned: 348 Modelled: 362 Confidence: 100.0% Identity: 27% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative cytoplasmic protein; PDBTitle: structure of np459575, a predicted glutathione synthase from2 salmonella typhimurium Phyre2 PDB 2gwc chain E Region: 13 - 370 Aligned: 350 Modelled: 354 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: E: PDB Molecule:glutamate cysteine ligase; PDBTitle: crystal structure of plant glutamate cysteine ligase in complex with a2 transition state analogue Phyre2 PDB 3nzt chain A Region: 11 - 360 Aligned: 348 Modelled: 350 Confidence: 100.0% Identity: 16% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: 2.0 angstrom crystal structure of glutamate--cysteine ligase (gsha)2 ftom francisella tularensis in complex with amp Phyre2 PDB 2d32 chain A domain 1 Region: 17 - 345 Aligned: 326 Modelled: 329 Confidence: 100.0% Identity: 15% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase Phyre2 PDB 3ln7 chain A Region: 19 - 374 Aligned: 341 Modelled: 356 Confidence: 100.0% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 pasteurella multocida Phyre2 PDB 3ln6 chain A Region: 19 - 374 Aligned: 340 Modelled: 355 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 streptococcus agalactiae Phyre2 PDB 3lvw chain A Region: 13 - 292 Aligned: 276 Modelled: 275 Confidence: 99.8% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: glutathione-inhibited scgcl Phyre2 PDB 4hpp chain A Region: 18 - 287 Aligned: 220 Modelled: 235 Confidence: 99.2% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:probable glutamine synthetase; PDBTitle: crystal structure of novel glutamine synthase homolog Phyre2 PDB 5zlp chain H Region: 15 - 283 Aligned: 204 Modelled: 206 Confidence: 99.1% Identity: 22% PDB header:ligase Chain: H: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from helicobacter pylori Phyre2 PDB 2bvc chain A domain 2 Region: 18 - 283 Aligned: 210 Modelled: 222 Confidence: 99.1% Identity: 20% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 1f52 chain A domain 2 Region: 15 - 263 Aligned: 195 Modelled: 204 Confidence: 99.0% Identity: 19% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 1fpy chain E Region: 15 - 282 Aligned: 212 Modelled: 223 Confidence: 99.0% Identity: 19% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from salmonella2 typhimurium with inhibitor phosphinothricin Phyre2 PDB 3ng0 chain A Region: 15 - 263 Aligned: 195 Modelled: 205 Confidence: 99.0% Identity: 20% PDB header:ligase Chain: A: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from synechocystis sp. pcc2 6803 Phyre2 PDB 4s17 chain E Region: 15 - 277 Aligned: 188 Modelled: 190 Confidence: 99.0% Identity: 22% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: the crystal structure of glutamine synthetase from bifidobacterium2 adolescentis atcc 15703 Phyre2 PDB 1hto chain B Region: 15 - 282 Aligned: 212 Modelled: 224 Confidence: 98.9% Identity: 20% PDB header:ligase Chain: B: PDB Molecule:glutamine synthetase; PDBTitle: crystallographic structure of a relaxed glutamine synthetase from2 mycobacterium tuberculosis Phyre2 PDB 5dm3 chain A Region: 18 - 287 Aligned: 208 Modelled: 211 Confidence: 98.9% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:l-glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from chromohalobacter2 salexigens dsm 3043(csal_0679, target efi-550015) with bound adp Phyre2 PDB 3qaj chain L Region: 3 - 263 Aligned: 206 Modelled: 215 Confidence: 98.9% Identity: 17% PDB header:ligase Chain: L: PDB Molecule:glutamine synthetase; PDBTitle: x-ray crystal structure of glutamine synthetase from bacillus subtilis2 cocrystallized with atp Phyre2 PDB 3o6x chain C Region: 18 - 263 Aligned: 193 Modelled: 203 Confidence: 98.7% Identity: 20% PDB header:ligase Chain: C: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the type iii glutamine synthetase from2 bacteroides fragilis Phyre2 1 d1r8ga_ 2 d1tt4a_ 3 c1tt4B_ 4 c2gwcE_ 5 c3nztA_ 6 d2d32a1 7 c3ln7A_ 8 c3ln6A_ 9 c3lvwA_ 10 c4hppA_ 11 c5zlpH_ 12 d2bvca2 13 d1f52a2 14 c1fpyE_ 15 c3ng0A_ 16 c4s17E_ 17 c1htoB_ 18 c5dm3A_ 19 c3qajL_ 20 c3o6xC_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71