1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	A	R	R	P	R	P	D	G	P	Q	H	L	L	A	L	V	R	S	A	V	P	P	V	H	P	A	G	R	P	F	I	A	A	G	L	A	I	A	A	V	G	H	R	Y	R	W	L	R	G	T	G	L	L	A	A	A	A	C	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	F	F	R	H	P	Q	R	V	P	P	T	R	P	A	A	I	V	A	P	A	D	G	V	I	C	A	I	D	S	A	A	P	P	A	E	L	S	M	G	D	T	P	L	P	R	V	S	I	F	L	S	I	L	D	A	H	V	Q	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	P	V	S	G	E	V	I	A	V	Q	H	R	P	G	R	F	G	S	A	D	L	P	E	A	S	D	D	N	E	R	T	S	V	R	I	R	M	P	N	G	A	E	V	V	A	V	Q	I	A	G	L	V	A	R	R	I	V	C	D
Secondary structure
SS confidence
Disorder																							?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.
Sequence		A	H	V	G	D	K	L	A	I	G	D	T	Y	G	L	I	R	F	G	S	R	L	D	T	Y	L	P	A	G	A	E	P	I	V	N	V	G	Q	R	A	V	A	G	E	T	V	L	A	E	C	R
Secondary structure
SS confidence
Disorder								?		?	?																																									?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 8%) Alpha helix ( 20%) Beta strand ( 41%) TM helix ( 20%) (View membrane topology prediction)

Rank Aligned region

PDB 1gla chain F Region: 71 - 231 Aligned: 138 Modelled: 144 Confidence: 98.4% Identity: 17% Fold: Barrel-sandwich hybrid Superfamily: Duplicated hybrid motif Family: Glucose permease-like Phyre2 PDB 2gpr chain A Region: 77 - 227 Aligned: 129 Modelled: 134 Confidence: 98.4% Identity: 16% Fold: Barrel-sandwich hybrid Superfamily: Duplicated hybrid motif Family: Glucose permease-like Phyre2 PDB 2f3g chain A Region: 77 - 231 Aligned: 133 Modelled: 134 Confidence: 98.3% Identity: 17% Fold: Barrel-sandwich hybrid Superfamily: Duplicated hybrid motif Family: Glucose permease-like Phyre2 PDB 1gpr chain A Region: 73 - 231 Aligned: 137 Modelled: 142 Confidence: 98.2% Identity: 13% Fold: Barrel-sandwich hybrid Superfamily: Duplicated hybrid motif Family: Glucose permease-like Phyre2 PDB 2ejg chain D Region: 117 - 227 Aligned: 62 Modelled: 62 Confidence: 96.4% Identity: 34% PDB header:ligase Chain: D: PDB Molecule:149aa long hypothetical methylmalonyl-coa decarboxylase PDBTitle: crystal structure of the biotin protein ligase (mutation r48a) and2 biotin carboxyl carrier protein complex from pyrococcus horikoshii3 ot3 Phyre2 PDB 2auk chain A Region: 119 - 229 Aligned: 102 Modelled: 107 Confidence: 96.0% Identity: 25% PDB header:transferase Chain: A: PDB Molecule:dna-directed rna polymerase beta' chain; PDBTitle: structure of e. coli rna polymerase beta' g/g' insert Phyre2 PDB 4hnv chain B Region: 124 - 227 Aligned: 100 Modelled: 104 Confidence: 95.2% Identity: 11% PDB header:ligase Chain: B: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of r54e mutant of s. aureus pyruvate carboxylase Phyre2 PDB 3n6r chain K Region: 66 - 141 Aligned: 71 Modelled: 76 Confidence: 95.1% Identity: 18% PDB header:ligase Chain: K: PDB Molecule:propionyl-coa carboxylase, alpha subunit; PDBTitle: crystal structure of the holoenzyme of propionyl-coa carboxylase (pcc) Phyre2 PDB 2b8g chain A Region: 169 - 227 Aligned: 59 Modelled: 59 Confidence: 95.0% Identity: 15% PDB header:biosynthetic protein Chain: A: PDB Molecule:biotin/lipoyl attachment protein; PDBTitle: solution structure of bacillus subtilis blap biotinylated-2 form (energy minimized mean structure) Phyre2 PDB 3hbl chain A Region: 168 - 227 Aligned: 60 Modelled: 60 Confidence: 94.9% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of s. aureus pyruvate carboxylase t908a mutant Phyre2 PDB 3bg5 chain B Region: 168 - 227 Aligned: 60 Modelled: 60 Confidence: 94.8% Identity: 15% PDB header:ligase Chain: B: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of staphylococcus aureus pyruvate carboxylase Phyre2 PDB 5vz0 chain D Region: 168 - 227 Aligned: 60 Modelled: 60 Confidence: 94.7% Identity: 17% PDB header:ligase Chain: D: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of lactococcus lactis pyruvate carboxylase g746a2 mutant in complex with cyclic-di-amp Phyre2 PDB 1dcz chain A Region: 168 - 227 Aligned: 60 Modelled: 60 Confidence: 94.5% Identity: 17% Fold: Barrel-sandwich hybrid Superfamily: Single hybrid motif Family: Biotinyl/lipoyl-carrier proteins and domains Phyre2 PDB 1bdo chain A Region: 179 - 227 Aligned: 49 Modelled: 49 Confidence: 94.4% Identity: 22% Fold: Barrel-sandwich hybrid Superfamily: Single hybrid motif Family: Biotinyl/lipoyl-carrier proteins and domains Phyre2 PDB 4qsk chain B Region: 124 - 227 Aligned: 104 Modelled: 104 Confidence: 94.3% Identity: 18% PDB header:ligase Chain: B: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of l. monocytogenes pyruvate carboxylase in complex2 with cyclic-di-amp Phyre2 PDB 3va7 chain A Region: 168 - 227 Aligned: 60 Modelled: 60 Confidence: 94.3% Identity: 22% PDB header:ligase Chain: A: PDB Molecule:klla0e08119p; PDBTitle: crystal structure of the kluyveromyces lactis urea carboxylase Phyre2 PDB 5ks8 chain D Region: 168 - 227 Aligned: 60 Modelled: 60 Confidence: 94.2% Identity: 23% PDB header:ligase Chain: D: PDB Molecule:pyruvate carboxylase subunit beta; PDBTitle: crystal structure of two-subunit pyruvate carboxylase from2 methylobacillus flagellatus Phyre2 PDB 2ejm chain A Region: 177 - 227 Aligned: 51 Modelled: 51 Confidence: 93.7% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:methylcrotonoyl-coa carboxylase subunit alpha; PDBTitle: solution structure of ruh-072, an apo-biotnyl domain form2 human acetyl coenzyme a carboxylase Phyre2 PDB 1ghj chain A Region: 180 - 227 Aligned: 48 Modelled: 48 Confidence: 93.2% Identity: 21% Fold: Barrel-sandwich hybrid Superfamily: Single hybrid motif Family: Biotinyl/lipoyl-carrier proteins and domains Phyre2 PDB 1iyu chain A Region: 177 - 227 Aligned: 51 Modelled: 51 Confidence: 92.9% Identity: 16% Fold: Barrel-sandwich hybrid Superfamily: Single hybrid motif Family: Biotinyl/lipoyl-carrier proteins and domains Phyre2 1 d1glaf_ 2 d2gpra_ 3 d2f3ga_ 4 d1gpra_ 5 c2ejgD_ 6 c2aukA_ 7 c4hnvB_ 8 c3n6rK_ 9 c2b8gA_ 10 c3hblA_ 11 c3bg5B_ 12 c5vz0D_ 13 d1dcza_ 14 d1bdoa_ 15 c4qskB_ 16 c3va7A_ 17 c5ks8D_ 18 c2ejmA_ 19 d1ghja_ 20 d1iyua_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189