1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	E	H	T	D	F	E	L	L	E	L	A	T	P	Y	A	L	N	A	V	S	D	D	E	R	A	D	I	D	R	R	V	A	A	A	P	S	P	V	A	A	A	F	N	D	E	V	R	A	V	R	E	T	M	A	V	V	S	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																																																			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	T	T	A	E	P	P	A	H	L	R	T	A	I	L	D	A	T	K	P	E	V	R	R	Q	S	R	W	R	T	A	A	F	A	S	A	A	A	I	A	V	G	L	G	A	F	G	L	G	V	L	T	R	P	S	P	P	P	T	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?										?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																			?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	E	Q	V	L	T	A	P	D	V	R	T	V	S	R	P	L	G	A	G	T	A	T	V	V	F	S	R	D	R	N	T	G	L	L	V	M	N	N	V	A	P	P	S	R	G	T	V	Y	Q	M	W	L	L	G	G	A	K	G	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																						?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.
Sequence		R	S	A	G	T	M	G	T	A	A	V	T	P	S	T	T	A	T	L	T	D	L	G	A	S	T	A	L	A	F	T	V	E	P	G	T	G	S	P	Q	P	T	G	T	I	L	A	E	L	P	L	G
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?	?	?												?	?	?										?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 39%) Alpha helix ( 47%) Beta strand ( 17%) TM helix ( 7%) (View membrane topology prediction)

Rank Aligned region

PDB 3vdo chain B Region: 7 - 79 Aligned: 73 Modelled: 73 Confidence: 99.6% Identity: 100% PDB header:dna binding protein/protein binding Chain: B: PDB Molecule:anti-sigma-k factor rska; PDBTitle: structure of extra-cytoplasmic function(ecf) sigma factor sigk in2 complex with its negative regulator rska from mycobacterium3 tuberculosis Phyre2 PDB 2z2s chain D Region: 1 - 85 Aligned: 78 Modelled: 85 Confidence: 99.3% Identity: 12% PDB header:transcription Chain: D: PDB Molecule:anti-sigma factor chrr, transcriptional activator chrr; PDBTitle: crystal structure of rhodobacter sphaeroides sige in complex with the2 anti-sigma chrr Phyre2 PDB 5wuq chain D Region: 6 - 78 Aligned: 68 Modelled: 68 Confidence: 99.1% Identity: 7% PDB header:metal binding protein Chain: D: PDB Molecule:anti-sigma-w factor rsiw; PDBTitle: crystal structure of sigw in complex with its anti-sigma rsiw, a zinc2 binding form Phyre2 PDB 5frh chain A Region: 2 - 89 Aligned: 88 Modelled: 88 Confidence: 98.9% Identity: 7% PDB header:transcription Chain: A: PDB Molecule:anti-sigma factor rsra; PDBTitle: solution structure of oxidised rsra Phyre2 PDB 3hug chain J Region: 3 - 63 Aligned: 58 Modelled: 61 Confidence: 98.8% Identity: 16% PDB header:transcription/membrane protein Chain: J: PDB Molecule:probable conserved membrane protein; PDBTitle: crystal structure of mycobacterium tuberculosis anti-sigma factor rsla2 in complex with -35 promoter binding domain of sigl Phyre2 PDB 5cam chain C Region: 19 - 53 Aligned: 35 Modelled: 35 Confidence: 67.7% Identity: 29% PDB header:transcription Chain: C: PDB Molecule:pupr protein; PDBTitle: crystal structure of the cytoplasmic domain of the pseudomonas putida2 anti-sigma factor pupr (semet) Phyre2 PDB 1or7 chain C Region: 10 - 81 Aligned: 64 Modelled: 67 Confidence: 34.4% Identity: 13% Fold: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Superfamily: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Family: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Phyre2 PDB 1or7 chain C Region: 10 - 81 Aligned: 64 Modelled: 67 Confidence: 34.4% Identity: 13% PDB header:transcription Chain: C: PDB Molecule:sigma-e factor negative regulatory protein; PDBTitle: crystal structure of escherichia coli sigmae with the cytoplasmic2 domain of its anti-sigma rsea Phyre2 PDB 5ee2 chain A Region: 163 - 175 Aligned: 13 Modelled: 13 Confidence: 30.5% Identity: 38% PDB header:metal transport Chain: A: PDB Molecule:hemoglobin-haptoglobin-utilization protein; PDBTitle: the crystal structure of the c-terminal beta-barrel of hpua from2 neisseria gonorrhoeae Phyre2 PDB 2mjl chain A Region: 13 - 45 Aligned: 33 Modelled: 33 Confidence: 22.5% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:peptidyl-trna hydrolase; PDBTitle: solution structure of peptidyl-trna hyrolase from vibrio cholerae Phyre2 PDB 5j84 chain A Region: 5 - 39 Aligned: 33 Modelled: 35 Confidence: 14.6% Identity: 9% PDB header:lyase Chain: A: PDB Molecule:dihydroxy-acid dehydratase; PDBTitle: crystal structure of l-arabinonate dehydratase in holo-form Phyre2 PDB 2pth chain A Region: 13 - 45 Aligned: 33 Modelled: 33 Confidence: 14.6% Identity: 18% Fold: Phosphorylase/hydrolase-like Superfamily: Peptidyl-tRNA hydrolase-like Family: Peptidyl-tRNA hydrolase-like Phyre2 PDB 5ze4 chain A Region: 5 - 39 Aligned: 33 Modelled: 35 Confidence: 13.5% Identity: 12% PDB header:lyase Chain: A: PDB Molecule:dihydroxy-acid dehydratase, chloroplastic; PDBTitle: the structure of holo- structure of dhad complex with [2fe-2s] cluster Phyre2 PDB 4jd9 chain B Region: 19 - 38 Aligned: 20 Modelled: 20 Confidence: 12.8% Identity: 5% PDB header:protein binding Chain: B: PDB Molecule:14.5 kda salivary protein; PDBTitle: contact pathway inhibitor from a sand fly Phyre2 PDB 5oyn chain B Region: 5 - 39 Aligned: 33 Modelled: 35 Confidence: 11.4% Identity: 18% PDB header:lyase Chain: B: PDB Molecule:dehydratase, ilvd/edd family; PDBTitle: crystal structure of d-xylonate dehydratase in holo-form Phyre2 PDB 1j2n chain A Region: 1 - 37 Aligned: 32 Modelled: 37 Confidence: 11.4% Identity: 22% Fold: Myosin phosphatase inhibitor 17kDa protein, CPI-17 Superfamily: Myosin phosphatase inhibitor 17kDa protein, CPI-17 Family: Myosin phosphatase inhibitor 17kDa protein, CPI-17 Phyre2 PDB 5ja9 chain D Region: 21 - 38 Aligned: 18 Modelled: 18 Confidence: 11.2% Identity: 22% PDB header:toxin Chain: D: PDB Molecule:toxin higb-2; PDBTitle: crystal structure of the higb2 toxin in complex with nb6 Phyre2 PDB 5lbm chain D Region: 2 - 36 Aligned: 34 Modelled: 35 Confidence: 11.2% Identity: 6% PDB header:transcription Chain: D: PDB Molecule:transcriptional repressor frmr; PDBTitle: the asymmetric tetrameric structure of the formaldehyde sensing2 transcriptional repressor frmr from escherichia coli Phyre2 PDB 1k5o chain A Region: 1 - 37 Aligned: 32 Modelled: 37 Confidence: 11.0% Identity: 22% Fold: Myosin phosphatase inhibitor 17kDa protein, CPI-17 Superfamily: Myosin phosphatase inhibitor 17kDa protein, CPI-17 Family: Myosin phosphatase inhibitor 17kDa protein, CPI-17 Phyre2 PDB 1wrd chain A domain 1 Region: 9 - 46 Aligned: 38 Modelled: 38 Confidence: 10.6% Identity: 5% Fold: Spectrin repeat-like Superfamily: GAT-like domain Family: GAT domain Phyre2 1 c3vdoB_ 2 c2z2sD_ 3 c5wuqD_ 4 c5frhA_ 5 c3hugJ_ 6 c5camC_ 7 d1or7c_ 8 c1or7C_ 9 c5ee2A_ 10 c2mjlA_ 11 c5j84A_ 12 d2ptha_ 13 c5ze4A_ 14 c4jd9B_ 15 c5oynB_ 16 d1j2na_ 17 c5ja9D_ 18 c5lbmD_ 19 d1k5oa_ 20 d1wrda1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36