1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	T	P	D	F	Q	T	L	L	Y	T	T	A	G	P	V	A	T	I	T	L	N	R	P	E	Q	L	N	T	I	V	P	P	M	P	D	E	I	E	A	A	I	G	L	A	E	R	D	Q	D	I	K	V	I	V	L	R	G	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	R	A	F	S	G	G	Y	D	F	G	G	G	F	Q	H	W	G	D	A	M	M	T	D	G	R	W	D	P	G	K	D	F	A	M	V	T	A	R	E	T	G	P	T	Q	K	F	M	A	I	W	R	A	S	K	P	V	I	A	Q
Secondary structure
SS confidence
Disorder															?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	H	G	W	C	V	G	G	A	S	D	Y	A	L	C	A	D	I	V	I	A	S	E	D	A	V	I	G	T	P	Y	S	R	M	W	G	A	Y	L	T	G	M	W	L	Y	R	L	S	L	A	K	V	K	W	H	S	L	T	G	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		P	L	T	G	V	Q	A	A	E	A	E	L	I	N	E	A	V	P	F	E	R	L	E	A	R	V	A	E	I	A	T	E	L	A	R	I	P	L	S	Q	L	Q	A	Q	K	L	I	V	N	Q	A	Y	E	N	M	G	L	A	S	T
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Q	L	L	G	G	I	L	D	G	L	M	R	N	T	P	D	A	L	E	F	I	R	T	A	Q	T	Q	G	V	R	A	A	V	E	R	R	D	G	P	F	G	D	Y	S	Q	A	P	P	E	L	R	P	D	P	T	H	V	I	T	P
Secondary structure
SS confidence
Disorder																																										?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.
Sequence		D	G	S	M
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 16%) Alpha helix ( 49%) Beta strand ( 11%) TM helix ( 7%) (View membrane topology prediction)

Rank Aligned region

PDB 3njb chain A Region: 1 - 289 Aligned: 289 Modelled: 289 Confidence: 100.0% Identity: 36% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase; PDBTitle: crystal structure of enoyl-coa hydratase from mycobacterium smegmatis,2 iodide soak Phyre2 PDB 3g64 chain A Region: 2 - 283 Aligned: 262 Modelled: 282 Confidence: 100.0% Identity: 23% PDB header:lyase Chain: A: PDB Molecule:putative enoyl-coa hydratase; PDBTitle: crystal structure of putative enoyl-coa hydratase from streptomyces2 coelicolor a3(2) Phyre2 PDB 4jcs chain A Region: 3 - 287 Aligned: 262 Modelled: 271 Confidence: 100.0% Identity: 22% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of enoyl-coa hydratase/isomerase from cupriavidus2 metallidurans ch34 Phyre2 PDB 5jbx chain B Region: 3 - 283 Aligned: 257 Modelled: 266 Confidence: 100.0% Identity: 23% PDB header:lyase Chain: B: PDB Molecule:3-hydroxybutyryl-coa dehydratase; PDBTitle: crystal structure of liuc in complex with coenzyme a and malonic acid Phyre2 PDB 3t88 chain A Region: 2 - 287 Aligned: 263 Modelled: 273 Confidence: 100.0% Identity: 25% PDB header:lyase/lyase inhibitor Chain: A: PDB Molecule:1,4-dihydroxy-2-naphthoyl-coa synthase; PDBTitle: crystal structure of escherichia coli menb in complex with substrate2 analogue, osb-ncoa Phyre2 PDB 3i47 chain A Region: 5 - 281 Aligned: 257 Modelled: 277 Confidence: 100.0% Identity: 19% PDB header:lyase Chain: A: PDB Molecule:enoyl coa hydratase/isomerase (crotonase); PDBTitle: crystal structure of putative enoyl coa hydratase/isomerase2 (crotonase) from legionella pneumophila subsp. pneumophila str.3 philadelphia 1 Phyre2 PDB 4i4z chain E Region: 3 - 292 Aligned: 267 Modelled: 290 Confidence: 100.0% Identity: 27% PDB header:lyase Chain: E: PDB Molecule:naphthoate synthase; PDBTitle: synechocystis sp. pcc 6803 1,4-dihydroxy-2-naphthoyl-coenzyme a2 synthase (menb) in complex with salicylyl-coa Phyre2 PDB 4j2u chain A Region: 1 - 301 Aligned: 274 Modelled: 292 Confidence: 100.0% Identity: 23% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase; PDBTitle: crystal structure of an enoyl-coa hydratase from rhodobacter2 sphaeroides 2.4.1 Phyre2 PDB 4zu2 chain A Region: 7 - 282 Aligned: 255 Modelled: 276 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:putative isohexenylglutaconyl-coa hydratase; PDBTitle: pseudomonas aeruginosa atue Phyre2 PDB 2vx2 chain D Region: 7 - 282 Aligned: 253 Modelled: 262 Confidence: 100.0% Identity: 20% PDB header:isomerase Chain: D: PDB Molecule:enoyl-coa hydratase domain-containing protein 3; PDBTitle: crystal structure of human enoyl coenzyme a hydratase2 domain-containing protein 3 (echdc3) Phyre2 PDB 4k29 chain A Region: 5 - 281 Aligned: 265 Modelled: 277 Confidence: 100.0% Identity: 30% PDB header:isomerase Chain: A: PDB Molecule:enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of an enoyl-coa hydratase/isomerase from2 xanthobacter autotrophicus py2 Phyre2 PDB 4hdt chain A Region: 7 - 301 Aligned: 263 Modelled: 263 Confidence: 100.0% Identity: 24% PDB header:isomerase Chain: A: PDB Molecule:3-hydroxyisobutyryl-coa hydrolase; PDBTitle: crystal structure of a carnitinyl-coa dehydratase from mycobacterium2 thermoresistibile Phyre2 PDB 2iex chain A Region: 5 - 287 Aligned: 244 Modelled: 254 Confidence: 100.0% Identity: 32% PDB header:lyase Chain: A: PDB Molecule:dihydroxynapthoic acid synthetase; PDBTitle: crystal structure of dihydroxynapthoic acid synthetase (gk2873) from2 geobacillus kaustophilus hta426 Phyre2 PDB 4jfc chain A Region: 8 - 281 Aligned: 253 Modelled: 274 Confidence: 100.0% Identity: 22% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase; PDBTitle: crystal structure of a enoyl-coa hydratase from polaromonas sp. js666 Phyre2 PDB 3moy chain A Region: 5 - 283 Aligned: 253 Modelled: 262 Confidence: 100.0% Identity: 29% PDB header:lyase Chain: A: PDB Molecule:probable enoyl-coa hydratase; PDBTitle: crystal structure of probable enoyl-coa hydratase from mycobacterium2 smegmatis Phyre2 PDB 3bpt chain A Region: 3 - 301 Aligned: 270 Modelled: 291 Confidence: 100.0% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:3-hydroxyisobutyryl-coa hydrolase; PDBTitle: crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in2 complex with quercetin Phyre2 PDB 2qq3 chain F Region: 6 - 283 Aligned: 252 Modelled: 261 Confidence: 100.0% Identity: 28% PDB header:lyase Chain: F: PDB Molecule:enoyl-coa hydratase subunit i; PDBTitle: crystal structure of enoyl-coa hydrates subunit i (gk_2039)2 other form from geobacillus kaustophilus hta426 Phyre2 PDB 4mi2 chain C Region: 8 - 283 Aligned: 256 Modelled: 276 Confidence: 100.0% Identity: 21% PDB header:isomerase Chain: C: PDB Molecule:putative enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of putative enoyl-coa hydratase/isomerase from2 mycobacterium abscessus Phyre2 PDB 5zai chain B Region: 5 - 281 Aligned: 253 Modelled: 262 Confidence: 100.0% Identity: 30% PDB header:hydrolase Chain: B: PDB Molecule:3-hydroxypropionyl-coenzyme a dehydratase; PDBTitle: crystal strcuture of 3-hydroxypropionyl-coa dehydratase from2 metallosphaera sedula Phyre2 PDB 1nzy chain A Region: 6 - 285 Aligned: 261 Modelled: 280 Confidence: 100.0% Identity: 18% Fold: ClpP/crotonase Superfamily: ClpP/crotonase Family: Crotonase-like Phyre2 1 c3njbA_ 2 c3g64A_ 3 c4jcsA_ 4 c5jbxB_ 5 c3t88A_ 6 c3i47A_ 7 c4i4zE_ 8 c4j2uA_ 9 c4zu2A_ 10 c2vx2D_ 11 c4k29A_ 12 c4hdtA_ 13 c2iexA_ 14 c4jfcA_ 15 c3moyA_ 16 c3bptA_ 17 c2qq3F_ 18 c4mi2C_ 19 c5zaiB_ 20 d1nzya_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208