1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	A	L	F	T	T	A	M	A	L	R	P	L	D	S	D	P	G	N	P	A	C	R	V	F	E	G	E	L	N	E	H	W	T	I	G	P	K	V	H	G	G	A	M	V	A	L	C	A	N	A	A	R	T	A	Y	G	A	A
Secondary structure
SS confidence
Disorder		?	?	?	?							?	?	?	?	?	?	?	?	?	?	?																																					?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	Q	Q	P	M	R	Q	P	V	A	V	S	A	S	F	L	W	A	P	D	P	G	T	M	R	L	V	T	S	I	R	K	R	G	R	R	I	S	V	A	D	V	E	L	T	Q	G	G	R	T	A	V	H	A	V	V	T	L	G	E
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		P	E	H	F	L	P	G	V	D	G	S	G	G	A	S	G	T	A	P	L	L	S	A	N	P	V	V	E	L	M	A	P	E	P	P	E	G	V	V	P	I	G	P	G	H	Q	L	A	G	L	V	H	L	G	E	G	C	D	V	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?				?	?	?	?																?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		P	V	L	S	T	L	R	S	A	T	D	G	R	P	P	V	I	Q	L	W	A	R	P	R	G	V	A	P	D	A	L	F	A	L	L	C	G	D	L	S	A	P	V	T	F	A	V	D	R	T	G	W	A	P	T	V	A	L	T	A
Secondary structure
SS confidence
Disorder					?	?	?	?	?	?	?	?	?	?	?	?																								?			?		?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.
Sequence		Y	L	R	A	L	P	A	D	G	W	L	R	V	L	C	T	C	V	E	I	G	Q	D	W	F	D	E	D	H	I	V	V	D	R	L	G	R	I	V	V	Q	T	R	Q	L	A	M	V	P	A	Q
Secondary structure
SS confidence
Disorder																			?		?	?	?																							?		?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 29%) Alpha helix ( 12%) Beta strand ( 36%) TM helix ( 5%) (View membrane topology prediction)

Rank Aligned region

PDB 3bbj chain A Region: 2 - 289 Aligned: 259 Modelled: 271 Confidence: 100.0% Identity: 25% PDB header:hydrolase Chain: A: PDB Molecule:putative thioesterase ii; PDBTitle: crystal structure of a putative thioesterase ii (tfu_2367) from2 thermobifida fusca yx at 2.45 a resolution Phyre2 PDB 3rqb chain B Region: 1 - 290 Aligned: 254 Modelled: 266 Confidence: 100.0% Identity: 19% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of conserved protein of unknown function with hot2 dog fold from alicyclobacillus acidocaldarius Phyre2 PDB 1c8u chain A Region: 5 - 291 Aligned: 264 Modelled: 287 Confidence: 100.0% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:acyl-coa thioesterase ii; PDBTitle: crystal structure of the e.coli thioesterase ii, a2 homologue of the human nef-binding enzyme Phyre2 PDB 3cjy chain A Region: 19 - 290 Aligned: 242 Modelled: 250 Confidence: 100.0% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:putative thioesterase; PDBTitle: crystal structure of putative thioesterase (yp_496845.1) from2 novosphingobium aromaticivorans dsm 12444 at 1.70 a resolution Phyre2 PDB 3rd7 chain A Region: 5 - 289 Aligned: 251 Modelled: 267 Confidence: 100.0% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:acyl-coa thioesterase; PDBTitle: crystal structure of acyl-coa thioesterase from mycobacterium avium Phyre2 PDB 4r9z chain B Region: 5 - 289 Aligned: 233 Modelled: 253 Confidence: 100.0% Identity: 21% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: mycobacterium avium subs paratuberculosis tesb protein map1729c Phyre2 PDB 1c8u chain A domain 2 Region: 139 - 291 Aligned: 153 Modelled: 153 Confidence: 99.9% Identity: 16% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: Acyl-CoA thioesterase Phyre2 PDB 1c8u chain A domain 1 Region: 5 - 124 Aligned: 106 Modelled: 120 Confidence: 99.9% Identity: 21% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: Acyl-CoA thioesterase Phyre2 PDB 1tbu chain A domain 1 Region: 7 - 119 Aligned: 94 Modelled: 113 Confidence: 99.8% Identity: 18% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: Acyl-CoA thioesterase Phyre2 PDB 2pim chain A Region: 25 - 118 Aligned: 94 Modelled: 94 Confidence: 99.5% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:phenylacetic acid degradation-related protein; PDBTitle: crystal structure of a putative thioesterase, phenylacetic acid2 degradation-related protein (reut_b4779) from ralstonia eutropha3 jmp134 at 2.20 a resolution Phyre2 PDB 2qwz chain B Region: 9 - 122 Aligned: 109 Modelled: 114 Confidence: 99.4% Identity: 20% PDB header:hydrolase Chain: B: PDB Molecule:phenylacetic acid degradation-related protein; PDBTitle: crystal structure of a putative thioesterase (tm1040_1390) from2 silicibacter sp. tm1040 at 2.15 a resolution Phyre2 PDB 1zki chain A domain 1 Region: 3 - 120 Aligned: 115 Modelled: 118 Confidence: 99.4% Identity: 22% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 4rmm chain A Region: 5 - 118 Aligned: 106 Modelled: 114 Confidence: 99.4% Identity: 20% PDB header:unknown function Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of the q7nvp2_chrvo protein from chromobacterium2 violaceum. northeast structural genomics consortium target cvr191 Phyre2 PDB 2f0x chain A domain 1 Region: 5 - 118 Aligned: 111 Modelled: 114 Confidence: 99.4% Identity: 19% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 1wlu chain A domain 1 Region: 5 - 120 Aligned: 108 Modelled: 109 Confidence: 99.4% Identity: 21% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 3f1t chain B Region: 5 - 120 Aligned: 113 Modelled: 116 Confidence: 99.4% Identity: 12% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein q9i3c8_pseae; PDBTitle: crystal structure of the q9i3c8_pseae protein from pseudomonas2 aeruginosa. northeast structural genomics consortium target par319a. Phyre2 PDB 3nwz chain D Region: 19 - 118 Aligned: 100 Modelled: 99 Confidence: 99.3% Identity: 14% PDB header:structural genomics, unknown function Chain: D: PDB Molecule:bh2602 protein; PDBTitle: crystal structure of bh2602 protein from bacillus halodurans with coa,2 northeast structural genomics consortium target bhr199 Phyre2 PDB 2hbo chain A domain 1 Region: 5 - 132 Aligned: 125 Modelled: 128 Confidence: 99.3% Identity: 14% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 3e8p chain A Region: 5 - 118 Aligned: 111 Modelled: 114 Confidence: 99.3% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the protein q8e9m7 from shewanella oneidensis2 related to thioesterase superfamily. northeast structural genomics3 consortium target sor246. Phyre2 PDB 2h4u chain A domain 1 Region: 5 - 118 Aligned: 112 Modelled: 114 Confidence: 99.3% Identity: 19% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 1 c3bbjA_ 2 c3rqbB_ 3 c1c8uA_ 4 c3cjyA_ 5 c3rd7A_ 6 c4r9zB_ 7 d1c8ua2 8 d1c8ua1 9 d1tbua1 10 c2pimA_ 11 c2qwzB_ 12 d1zkia1 13 c4rmmA_ 14 d2f0xa1 15 d1wlua1 16 c3f1tB_ 17 c3nwzD_ 18 d2hboa1 19 c3e8pA_ 20 d2h4ua1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174