1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	S	P	E	A	I	A	H	F	D	E	R	F	E	C	H	A	P	R	T	T	R	V	S	A	A	F	I	D	R	I	C	S	A	T	R	A	E	N	R	A	A	A	A	Q	L	V	A	L	G	E	L	F	A	Y	R	W	S	R
Secondary structure
SS confidence
Disorder		?	?	?						?	?							?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		C	G	G	R	E	E	W	V	M	D	T	M	A	A	V	A	A	E	V	A	A	A	L	R	I	S	Q	G	L	A	A	S	R	L	R	Y	A	R	A	M	R	E	R	L	P	K	T	A	E	V	F	S	A	G	D	I	G	Y	L	M
Secondary structure
SS confidence
Disorder				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		F	A	T	I	V	Y	R	T	D	L	I	V	D	P	D	V	L	A	A	V	D	A	Q	L	A	A	N	V	A	R	W	P	S	M	T	K	A	R	L	A	G	Q	V	D	K	I	V	A	R	A	D	A	D	A	V	R	R	R	K	E
Secondary structure
SS confidence
Disorder																																																									?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Y	Q	A	Q	R	Q	F	W	V	G	E	S	Q	D	G	V	C	Q	I	G	G	S	L	L	A	V	D	A	H	A	L	D	A	R	L	S	A	L	A	G	T	V	C	E	H	D	P	R	S	R	E	Q	R	R	A	D	A	L	G	A
Secondary structure
SS confidence
Disorder			?																																										?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	A	G	G	A	D	R	L	G	C	G	C	G	R	A	D	C	A	A	G	K	R	P	A	A	P	P	V	V	I	H	L	I	A	E	A	A	T	I	N	G	T	G	S	A	P	A	S	Q	M	N	A	D	G	L	I	T	A	E	L
Secondary structure
SS confidence
Disorder															?	?	?	?	?	?	?	?	?	?																	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	A	E	L	A	K	T	A	T	L	V	P	L	V	H	P	G	D	A	P	P	E	P	G	Y	A	P	S	K	A	L	A	D	F	V	R	C	R	D	L	T	C	R	W	P	G	C	D	E	P	A	T	N	C	D	L	D	H	T	I
Secondary structure
SS confidence
Disorder																						?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		P	Y	A	A	G	G	P	T	H	A	S	N	L	K	C	Y	C	R	T	H	H	L	V	K	T	F	W	G	W	R	D	Q	Q	L	P	D	G	T	L	I	L	T	S	P	S	G	H	T	Y	V	S	T	P	G	S	A	L	L	F	P
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		S	L	C	H	F	S	G	G	I	P	A	P	E	A	D	P	P	Y	D	H	C	D	Q	R	T	A	M	M	P	K	R	R	R	T	R	A	Q	D	R	A	Y	R	I	A	T	E	R	R	Q	N	H	A	A	R	Q	R	A	Q	V	L
Secondary structure
SS confidence
Disorder					?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?				?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.
Sequence		T	Q	T	A	A	A	T	D	T	H	G	P	P	P	D	H	N	D	D	P	P	P	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 22%) Alpha helix ( 43%) Beta strand ( 7%)

Rank Aligned region

PDB 2qgp chain A Region: 325 - 386 Aligned: 62 Modelled: 62 Confidence: 99.0% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease; PDBTitle: x-ray structure of the nhn endonuclease from geobacter2 metallireducens. northeast structural genomics consortium target3 gmr87. Phyre2 PDB 5mkw chain A Region: 328 - 385 Aligned: 58 Modelled: 58 Confidence: 98.7% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:dna annealing helicase and endonuclease zranb3; PDBTitle: crystal structure of the human zranb3 hnh domain Phyre2 PDB 6ghc chain A Region: 295 - 387 Aligned: 93 Modelled: 93 Confidence: 98.6% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:5-methylcytosine-specific restriction enzyme a; PDBTitle: modification dependent ecokmcra restriction endonuclease Phyre2 PDB 5x1h chain S Region: 329 - 404 Aligned: 73 Modelled: 76 Confidence: 98.5% Identity: 21% PDB header:protein transport Chain: S: PDB Molecule:icmj (dotn); PDBTitle: structure of legionella pneumophila dotn Phyre2 PDB 5h0m chain A Region: 329 - 385 Aligned: 57 Modelled: 57 Confidence: 98.1% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease; PDBTitle: crystal structure of deep-sea thermophilic bacteriophage gve2 hnh2 endonuclease with zinc ion Phyre2 PDB 5vgb chain A Region: 331 - 384 Aligned: 52 Modelled: 54 Confidence: 97.8% Identity: 13% PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:crispr-associated endonuclease cas9; PDBTitle: crystal structure of nmecas9 hnh domain bound to anti-crispr acriic1 Phyre2 PDB 5zmm chain D Region: 323 - 387 Aligned: 65 Modelled: 65 Confidence: 97.6% Identity: 26% PDB header:dna binding protein Chain: D: PDB Molecule:uncharacterized protein mcra; PDBTitle: structure of the type iv phosphorothioation-dependent restriction2 endonuclease scomcra Phyre2 PDB 4oge chain A Region: 329 - 384 Aligned: 56 Modelled: 56 Confidence: 96.9% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease domain protein; PDBTitle: crystal structure of the type ii-c cas9 enzyme from actinomyces2 naeslundii Phyre2 PDB 5axw chain A Region: 331 - 384 Aligned: 54 Modelled: 54 Confidence: 96.4% Identity: 19% PDB header:hydrolase/rna/dna Chain: A: PDB Molecule:crispr-associated endonuclease cas9; PDBTitle: crystal structure of staphylococcus aureus cas9 in complex with sgrna2 and target dna (ttgggt pam) Phyre2 PDB 6ghs chain A Region: 335 - 381 Aligned: 45 Modelled: 47 Confidence: 91.6% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:tagi restriction endonuclease; PDBTitle: modification dependent tagi restriction endonuclease Phyre2 PDB 1vz0 chain A domain 1 Region: 77 - 149 Aligned: 65 Modelled: 73 Confidence: 91.0% Identity: 20% Fold: DNA/RNA-binding 3-helical bundle Superfamily: KorB DNA-binding domain-like Family: KorB DNA-binding domain-like Phyre2 PDB 2ho2 chain A domain 1 Region: 388 - 414 Aligned: 27 Modelled: 27 Confidence: 82.8% Identity: 37% Fold: WW domain-like Superfamily: WW domain Family: WW domain Phyre2 PDB 2gyk chain B domain 1 Region: 355 - 387 Aligned: 33 Modelled: 33 Confidence: 82.7% Identity: 21% Fold: His-Me finger endonucleases Superfamily: His-Me finger endonucleases Family: HNH-motif Phyre2 PDB 3mky chain P Region: 77 - 163 Aligned: 81 Modelled: 87 Confidence: 81.7% Identity: 12% PDB header:dna binding protein/dna Chain: P: PDB Molecule:protein sopb; PDBTitle: structure of sopb(155-323)-18mer dna complex, i23 form Phyre2 PDB 2jb0 chain B domain 1 Region: 355 - 381 Aligned: 26 Modelled: 27 Confidence: 79.6% Identity: 27% Fold: His-Me finger endonucleases Superfamily: His-Me finger endonucleases Family: HNH-motif Phyre2 PDB 4cmq chain B Region: 339 - 384 Aligned: 43 Modelled: 46 Confidence: 78.8% Identity: 16% PDB header:hydrolase Chain: B: PDB Molecule:crispr-associated endonuclease cas9/csn1; PDBTitle: crystal structure of mn-bound s.pyogenes cas9 Phyre2 PDB 3mkz chain U Region: 77 - 163 Aligned: 81 Modelled: 87 Confidence: 77.7% Identity: 12% PDB header:dna-binding protein/dna Chain: U: PDB Molecule:protein sopb; PDBTitle: structure of sopb(155-272)-18mer complex, p21 form Phyre2 PDB 2ysc chain A domain 1 Region: 388 - 408 Aligned: 21 Modelled: 21 Confidence: 75.6% Identity: 43% Fold: WW domain-like Superfamily: WW domain Family: WW domain Phyre2 PDB 1r71 chain A Region: 78 - 164 Aligned: 79 Modelled: 87 Confidence: 70.9% Identity: 15% Fold: DNA/RNA-binding 3-helical bundle Superfamily: KorB DNA-binding domain-like Family: KorB DNA-binding domain-like Phyre2 PDB 1r71 chain B Region: 78 - 163 Aligned: 78 Modelled: 86 Confidence: 69.3% Identity: 14% PDB header:transcription/dna Chain: B: PDB Molecule:transcriptional repressor protein korb; PDBTitle: crystal structure of the dna binding domain of korb in complex with2 the operator dna Phyre2 1 c2qgpA_ 2 c5mkwA_ 3 c6ghcA_ 4 c5x1hS_ 5 c5h0mA_ 6 c5vgbA_ 7 c5zmmD_ 8 c4ogeA_ 9 c5axwA_ 10 c6ghsA_ 11 d1vz0a1 12 d2ho2a1 13 d2gykb1 14 c3mkyP_ 15 d2jb0b1 16 c4cmqB_ 17 c3mkzU_ 18 d2ysca1 19 d1r71a_ 20 c1r71B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99