1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	T	T	I	P	T	S	K	S	A	C	S	V	T	T	R	P	G	N	A	A	V	D	Y	G	G	A	Q	I	R	A	Y	L	H	H	L	A	T	V	V	T	I	R	G	E	I	D	A	A	N	V	E	Q	I	S	E	H	V	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	F	S	L	G	T	N	P	M	V	L	D	L	S	E	L	S	H	F	S	G	A	G	I	S	L	L	C	I	L	D	E	D	C	R	A	A	G	V	Q	W	A	L	V	A	S	P	A	V	V	E	Q	L	G	G	R	C	D	Q	G
Secondary structure
SS confidence
Disorder																																																										?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.
Sequence		E	H	E	S	M	F	P	M	A	R	S	V	H	K	A	L	H	D	L	A	D	A	I	D	R	R	R	Q	L	V	L	P	L	I	S	R	S	A
Secondary structure
SS confidence
Disorder																						?	?	?	?	?	?	?				?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 20%) Alpha helix ( 37%) Beta strand ( 25%)

Rank Aligned region

PDB 3t6o chain A Region: 26 - 140 Aligned: 112 Modelled: 115 Confidence: 99.9% Identity: 18% PDB header:transport protein Chain: A: PDB Molecule:sulfate transporter/antisigma-factor antagonist stas; PDBTitle: the structure of an anti-sigma-factor antagonist (stas) domain protein2 from planctomyces limnophilus. Phyre2 PDB 4hyl chain B Region: 25 - 140 Aligned: 113 Modelled: 116 Confidence: 99.9% Identity: 20% PDB header:transcription regulator Chain: B: PDB Molecule:stage ii sporulation protein; PDBTitle: the crystal structure of an anti-sigma-factor antagonist from2 haliangium ochraceum dsm 14365 Phyre2 PDB 1th8 chain B Region: 29 - 142 Aligned: 111 Modelled: 114 Confidence: 99.9% Identity: 21% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Anti-sigma factor antagonist SpoIIaa Phyre2 PDB 3f43 chain A Region: 32 - 141 Aligned: 107 Modelled: 110 Confidence: 99.9% Identity: 14% PDB header:transcription Chain: A: PDB Molecule:putative anti-sigma factor antagonist tm1081; PDBTitle: crystal structure of a putative anti-sigma factor antagonist (tm1081)2 from thermotoga maritima at 1.59 a resolution Phyre2 PDB 4xs5 chain D Region: 32 - 146 Aligned: 111 Modelled: 115 Confidence: 99.8% Identity: 9% PDB header:transport protein Chain: D: PDB Molecule:sulfate transporter/antisigma-factor antagonist stas; PDBTitle: crystal structure of sulfate transporter/antisigma-factor antagonist2 stas from dyadobacter fermentans dsm 18053 Phyre2 PDB 1vc1 chain A Region: 29 - 135 Aligned: 104 Modelled: 107 Confidence: 99.8% Identity: 26% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Anti-sigma factor antagonist SpoIIaa Phyre2 PDB 3mgl chain A Region: 36 - 145 Aligned: 107 Modelled: 110 Confidence: 99.8% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:sulfate permease family protein; PDBTitle: crystal structure of permease family protein from vibrio cholerae Phyre2 PDB 1auz chain A Region: 29 - 140 Aligned: 109 Modelled: 112 Confidence: 99.8% Identity: 18% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Anti-sigma factor antagonist SpoIIaa Phyre2 PDB 3oir chain A Region: 36 - 143 Aligned: 105 Modelled: 108 Confidence: 99.8% Identity: 14% PDB header:transport protein Chain: A: PDB Molecule:sulfate transporter sulfate transporter family protein; PDBTitle: crystal structure of sulfate transporter family protein from wolinella2 succinogenes Phyre2 PDB 2vy9 chain A Region: 36 - 141 Aligned: 103 Modelled: 106 Confidence: 99.8% Identity: 17% PDB header:gene regulation Chain: A: PDB Molecule:anti-sigma-factor antagonist; PDBTitle: molecular architecture of the stressosome, a signal2 integration and transduction hub Phyre2 PDB 1h4x chain A Region: 35 - 139 Aligned: 100 Modelled: 105 Confidence: 99.8% Identity: 16% Fold: SpoIIaa-like Superfamily: SpoIIaa-like Family: Anti-sigma factor antagonist SpoIIaa Phyre2 PDB 2kln chain A Region: 36 - 141 Aligned: 103 Modelled: 103 Confidence: 99.8% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:probable sulphate-transport transmembrane protein, cog0659; PDBTitle: solution structure of stas domain of rv1739c from m. tuberculosis Phyre2 PDB 3ny7 chain A Region: 28 - 139 Aligned: 108 Modelled: 112 Confidence: 99.7% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:sulfate transporter; PDBTitle: stas domain of ychm bound to acp Phyre2 PDB 5ezb chain B Region: 36 - 140 Aligned: 102 Modelled: 105 Confidence: 99.7% Identity: 14% PDB header:transport protein Chain: B: PDB Molecule:chicken prestin stas domain,chicken prestin stas domain; PDBTitle: chicken prestin stas domain Phyre2 PDB 3llo chain A Region: 36 - 141 Aligned: 97 Modelled: 106 Confidence: 99.6% Identity: 16% PDB header:motor protein Chain: A: PDB Molecule:prestin; PDBTitle: crystal structure of the stas domain of motor protein prestin (anion2 transporter slc26a5) Phyre2 PDB 6ic4 chain K Region: 41 - 128 Aligned: 84 Modelled: 88 Confidence: 99.5% Identity: 17% PDB header:protein transport Chain: K: PDB Molecule:ttg2e; PDBTitle: cryo-em structure of the a. baumannii mla complex at 8.7 a resolution Phyre2 PDB 3lkl chain B Region: 35 - 118 Aligned: 80 Modelled: 84 Confidence: 99.4% Identity: 18% PDB header:transport protein Chain: B: PDB Molecule:antisigma-factor antagonist stas; PDBTitle: crystal structure of the c-terminal domain of anti-sigma factor2 antagonist stas from rhodobacter sphaeroides Phyre2 PDB 5da0 chain A Region: 31 - 116 Aligned: 85 Modelled: 86 Confidence: 99.0% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:sulphate transporter; PDBTitle: structure of the the slc26 transporter slc26dg in complex with a2 nanobody Phyre2 PDB 3ih9 chain A Region: 20 - 104 Aligned: 85 Modelled: 85 Confidence: 96.8% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:salt-tolerant glutaminase; PDBTitle: crystal structure analysis of mglu in its tris form Phyre2 PDB 3bl4 chain B Region: 36 - 141 Aligned: 101 Modelled: 106 Confidence: 92.8% Identity: 19% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a protein with unknown function (arth_0117) from2 arthrobacter sp. fb24 at 2.20 a resolution Phyre2 1 c3t6oA_ 2 c4hylB_ 3 d1th8b_ 4 c3f43A_ 5 c4xs5D_ 6 d1vc1a_ 7 c3mglA_ 8 d1auza_ 9 c3oirA_ 10 c2vy9A_ 11 d1h4xa_ 12 c2klnA_ 13 c3ny7A_ 14 c5ezbB_ 15 c3lloA_ 16 c6ic4K_ 17 c3lklB_ 18 c5da0A_ 19 c3ih9A_ 20 c3bl4B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99