1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	L	G	G	S	D	P	A	L	V	A	V	P	T	Q	H	E	S	L	L	G	A	L	R	V	G	E	Q	I	D	D	D	V	A	L	V	V	T	T	S	G	T	T	G	P	P	K	G	A	M	L	T	A	A	A	L	T	A	S	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?											?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	A	A	H	D	R	L	G	G	P	G	S	W	L	L	A	V	P	P	Y	H	I	A	G	L	A	V	L	V	R	S	V	I	A	G	S	V	P	V	E	L	N	V	S	A	G	F	D	V	T	E	L	P	N	A	I	K	R	L	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	G	R	R	Y	T	S	L	V	A	A	Q	L	A	K	A	L	T	D	P	A	A	T	A	A	L	A	E	L	D	A	V	L	I	G	G	G	P	A	P	R	P	I	L	D	A	A	A	A	A	G	I	T	V	V	R	T	Y	G	M
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	E	T	S	G	G	C	V	Y	D	G	V	P	L	D	G	V	R	L	R	V	L	A	G	G	R	I	A	I	G	G	A	T	L	A	K	G	Y	R	N	P	V	S	P	D	P	F	A	E	P	G	W	F	H	T	D	D	L	G	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	E	S	G	D	S	G	V	L	T	V	L	G	R	A	D	E	A	I	S	T	G	G	F	T	V	L	P	Q	P	V	E	A	A	L	G	T	H	P	A	V	R	D	C	A	V	F	G	L	A	D	D	R	L	G	Q	R	V	V	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	I	V	V	G	D	G	C	P	P	P	T	L	E	A	L	R	A	H	V	A	R	T	L	D	V	T	A	A	P	R	E	L	H	V	V	N	V	L	P	R	R	G	I	G	K	V	D	R	A	A	L	V	R	R	F	A	G	E	A
Secondary structure
SS confidence
Disorder																																																										?	?	?	?
Disorder confidence
		.	.
Sequence		D	Q
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 6%) Alpha helix ( 30%) Beta strand ( 26%) TM helix ( 10%) (View membrane topology prediction)

Rank Aligned region

PDB 6eqo chain B Region: 21 - 358 Aligned: 338 Modelled: 338 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: B: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: tri-functional propionyl-coa synthase of erythrobacter sp. nap1 with2 bound nadp+ and phosphomethylphosphonic acid adenylate ester Phyre2 PDB 5gxd chain A Region: 6 - 362 Aligned: 357 Modelled: 357 Confidence: 100.0% Identity: 19% PDB header:lyase Chain: A: PDB Molecule:amp-dependent synthetase and ligase; PDBTitle: structure of acryloyl-coa lyase prpe from dinoroseobacter shibae dfl2 12 Phyre2 PDB 6p1j chain A Region: 27 - 352 Aligned: 322 Modelled: 326 Confidence: 100.0% Identity: 21% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 5es8 chain A Region: 2 - 354 Aligned: 339 Modelled: 353 Confidence: 100.0% Identity: 19% PDB header:ligase Chain: A: PDB Molecule:linear gramicidin synthetase subunit a; PDBTitle: crystal structure of the initiation module of lgra in the thiolation2 state Phyre2 PDB 1pg4 chain A Region: 2 - 361 Aligned: 360 Modelled: 360 Confidence: 100.0% Identity: 20% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5u89 chain A Region: 17 - 354 Aligned: 327 Modelled: 338 Confidence: 100.0% Identity: 22% PDB header:hydrolase/inhibitor Chain: A: PDB Molecule:amino acid adenylation domain protein; PDBTitle: crystal structure of a cross-module fragment from the dimodular nrps2 dhbf Phyre2 PDB 5ja2 chain A Region: 2 - 352 Aligned: 350 Modelled: 351 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 5msd chain A Region: 17 - 362 Aligned: 340 Modelled: 346 Confidence: 100.0% Identity: 20% PDB header:oxidoreductase Chain: A: PDB Molecule:carboxylic acid reductase; PDBTitle: structure of the a domain of carboxylic acid reductase (car) from2 nocardia iowensis in complex with amp and benzoic acid Phyre2 PDB 2vsq chain A Region: 2 - 362 Aligned: 354 Modelled: 361 Confidence: 100.0% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 5wmm chain A Region: 1 - 352 Aligned: 347 Modelled: 352 Confidence: 100.0% Identity: 21% PDB header:biosynthetic protein Chain: A: PDB Molecule:nrps; PDBTitle: crystal structure of an adenylation domain interrupted by a2 methylation domain (ama4) from nonribosomal peptide synthetase tios Phyre2 PDB 1ry2 chain A Region: 3 - 359 Aligned: 354 Modelled: 357 Confidence: 100.0% Identity: 22% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 4zxj chain A Region: 2 - 352 Aligned: 350 Modelled: 351 Confidence: 100.0% Identity: 20% PDB header:biosynthetic protein Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: crystal structure of holo-entf a nonribosomal peptide synthetase in2 the thioester-forming conformation Phyre2 PDB 4wd1 chain A Region: 17 - 359 Aligned: 335 Modelled: 343 Confidence: 100.0% Identity: 17% PDB header:ligase Chain: A: PDB Molecule:acetoacetate-coa ligase; PDBTitle: acetoacetyl-coa synthetase from streptomyces lividans Phyre2 PDB 5mss chain A Region: 16 - 361 Aligned: 340 Modelled: 345 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: A: PDB Molecule:thioester reductase domain-containing protein; PDBTitle: structure of the a-pcp didomain of carboxylic acid reductase (car)2 from segniliparus rugosus in complex with amp Phyre2 PDB 5ifi chain A Region: 19 - 359 Aligned: 337 Modelled: 341 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: crystal structure of acetyl-coa synthetase in complex with adenosine-2 5'-propylphosphate from cryptococcus neoformans h99 Phyre2 PDB 4zxi chain A Region: 14 - 352 Aligned: 326 Modelled: 339 Confidence: 100.0% Identity: 17% PDB header:biosynthetic protein Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: crystal structure of holo-ab3403 a four domain nonribosomal peptide2 synthetase bound to amp and glycine Phyre2 PDB 5mst chain A Region: 20 - 362 Aligned: 337 Modelled: 342 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:thioester reductase domain-containing protein; PDBTitle: structure of the a domain of carboxylic acid reductase (car) from2 segniliparus rugosus in complex with amp and a co-purified carboxylic3 acid Phyre2 PDB 1mdb chain A Region: 15 - 361 Aligned: 343 Modelled: 347 Confidence: 100.0% Identity: 21% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 3e7w chain A Region: 16 - 362 Aligned: 347 Modelled: 347 Confidence: 100.0% Identity: 17% PDB header:ligase Chain: A: PDB Molecule:d-alanine--poly(phosphoribitol) ligase subunit 1; PDBTitle: crystal structure of dlta: implications for the reaction mechanism of2 non-ribosomal peptide synthetase (nrps) adenylation domains Phyre2 PDB 3vnq chain A Region: 19 - 356 Aligned: 337 Modelled: 338 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: A: PDB Molecule:nrps adenylation protein cytc1; PDBTitle: co-crystal structure of nrps adenylation protein cytc1 with atp from2 streptomyces Phyre2 1 c6eqoB_ 2 c5gxdA_ 3 c6p1jA_ 4 c5es8A_ 5 d1pg4a_ 6 c5u89A_ 7 c5ja2A_ 8 c5msdA_ 9 c2vsqA_ 10 c5wmmA_ 11 d1ry2a_ 12 c4zxjA_ 13 c4wd1A_ 14 c5mssA_ 15 c5ifiA_ 16 c4zxiA_ 17 c5mstA_ 18 d1mdba_ 19 c3e7wA_ 20 c3vnqA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101