1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	T	V	L	L	D	A	N	V	L	I	A	L	V	V	A	E	H	V	H	H	D	A	A	A	D	W	L	M	A	S	D	T	G	F	A	T	C	P	M	T	Q	G	S	L	V	R	F	L	V	R	S	G	Q	S	A	A	A	A	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?													?																																?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	V	V	S	A	V	Q	C	T	S	R	H	E	F	W	P	D	A	L	S	F	A	G	V	E	V	A	G	V	V	G	H	R	Q	V	T	D	A	Y	L	A	Q	L	A	R	S	H	D	G	Q	L	A	T	L	D	S	G	L	A	H
Secondary structure
SS confidence
Disorder																											?	?		?	?	?																											?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.
Sequence		L	H	G	D	V	A	V	L	I	P	T	T	T
Secondary structure
SS confidence
Disorder		?				?					?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 19%) Alpha helix ( 41%) Beta strand ( 17%)

Rank Aligned region

PDB 3zvk chain C Region: 1 - 129 Aligned: 127 Modelled: 129 Confidence: 99.9% Identity: 10% PDB header:antitoxin/toxin/dna Chain: C: PDB Molecule:toxin of toxin-antitoxin system; PDBTitle: crystal structure of vapbc2 from rickettsia felis bound to2 a dna fragment from their promoter Phyre2 PDB 5x3t chain D Region: 2 - 130 Aligned: 129 Modelled: 127 Confidence: 99.8% Identity: 17% PDB header:antitoxin/toxin Chain: D: PDB Molecule:ribonuclease vapc26; PDBTitle: vapbc from mycobacterium tuberculosis Phyre2 PDB 3tnd chain C Region: 1 - 127 Aligned: 124 Modelled: 127 Confidence: 99.8% Identity: 14% PDB header:translation, toxin Chain: C: PDB Molecule:trna(fmet)-specific endonuclease vapc; PDBTitle: crystal structure of shigella flexneri vapbc toxin-antitoxin complex Phyre2 PDB 6nkl chain A Region: 1 - 127 Aligned: 126 Modelled: 127 Confidence: 99.8% Identity: 13% PDB header:antitoxin Chain: A: PDB Molecule:ribonuclease vapc; PDBTitle: 2.2 a resolution structure of vapbc-1 from nontypeable haemophilus2 influenzae Phyre2 PDB 2fe1 chain A domain 1 Region: 1 - 125 Aligned: 123 Modelled: 125 Confidence: 99.8% Identity: 18% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 2fe1 chain A Region: 1 - 125 Aligned: 123 Modelled: 125 Confidence: 99.8% Identity: 18% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved hypothetical protein pae0151; PDBTitle: crystal structure of pae0151 from pyrobaculum aerophilum Phyre2 PDB 5l6m chain C Region: 2 - 129 Aligned: 124 Modelled: 128 Confidence: 99.8% Identity: 16% PDB header:hydrolase Chain: C: PDB Molecule:ribonuclease vapc; PDBTitle: structure of caulobacter crescentus vapbc1 (vapb1deltac:vapc1 form) Phyre2 PDB 5wzf chain B Region: 2 - 130 Aligned: 125 Modelled: 127 Confidence: 99.8% Identity: 17% PDB header:hydrolase Chain: B: PDB Molecule:23s rrna-specific endonuclease vapc20; PDBTitle: crystal structure of mycobacterium tuberculosis vapc20 (rv2549c),2 sarcin-ricin loop cleaving toxin Phyre2 PDB 4xgr chain G Region: 3 - 130 Aligned: 123 Modelled: 127 Confidence: 99.8% Identity: 10% PDB header:toxin/antitoxin Chain: G: PDB Molecule:ribonuclease vapc30; PDBTitle: crystal structure of addiction module from mycobacterial species Phyre2 PDB 6a7v chain G Region: 2 - 131 Aligned: 125 Modelled: 130 Confidence: 99.8% Identity: 13% PDB header:toxin/antitoxin Chain: G: PDB Molecule:ribonuclease vapc11; PDBTitle: crystal structure of mycobacterium tuberculosis vapbc11 toxin-2 antitoxin complex Phyre2 PDB 4chg chain C Region: 2 - 130 Aligned: 124 Modelled: 129 Confidence: 99.8% Identity: 12% PDB header:toxin/antitoxin Chain: C: PDB Molecule:probable ribonuclease vapc15; PDBTitle: crystal structure of vapbc15 complex from mycobacterium tuberculosis Phyre2 PDB 2h1c chain A domain 1 Region: 3 - 130 Aligned: 125 Modelled: 128 Confidence: 99.7% Identity: 20% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 3h87 chain B Region: 2 - 129 Aligned: 120 Modelled: 128 Confidence: 99.7% Identity: 14% PDB header:toxin/antitoxin Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: rv0301 rv0300 toxin antitoxin complex from mycobacterium tuberculosis Phyre2 PDB 5sv2 chain A Region: 2 - 121 Aligned: 112 Modelled: 120 Confidence: 99.7% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:ribonuclease vapc21; PDBTitle: toxin vapc21 from mycobacterium tuberculosis Phyre2 PDB 1v96 chain A domain 1 Region: 2 - 131 Aligned: 121 Modelled: 130 Confidence: 99.6% Identity: 12% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 3dbo chain B Region: 2 - 127 Aligned: 113 Modelled: 126 Confidence: 99.6% Identity: 21% PDB header:toxin/antitoxin Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a member of the vapbc family of toxin-antitoxin2 systems, vapbc-5, from mycobacterium tuberculosis Phyre2 PDB 1y82 chain A domain 1 Region: 2 - 132 Aligned: 124 Modelled: 131 Confidence: 99.5% Identity: 13% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 1v8p chain A Region: 1 - 126 Aligned: 115 Modelled: 126 Confidence: 99.4% Identity: 22% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 1v8p chain K Region: 1 - 126 Aligned: 115 Modelled: 118 Confidence: 99.4% Identity: 22% PDB header:structural genomics, unknown function Chain: K: PDB Molecule:hypothetical protein pae2754; PDBTitle: crystal structure of pae2754 from pyrobaculum aerophilum Phyre2 PDB 1w8i chain A Region: 1 - 119 Aligned: 116 Modelled: 118 Confidence: 99.3% Identity: 14% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 1 c3zvkC_ 2 c5x3tD_ 3 c3tndC_ 4 c6nklA_ 5 d2fe1a1 6 c2fe1A_ 7 c5l6mC_ 8 c5wzfB_ 9 c4xgrG_ 10 c6a7vG_ 11 c4chgC_ 12 d2h1ca1 13 c3h87B_ 14 c5sv2A_ 15 d1v96a1 16 c3dboB_ 17 d1y82a1 18 d1v8pa_ 19 c1v8pK_ 20 d1w8ia_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44