1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	T	T	F	D	G	D	T	S	A	G	E	A	V	D	L	T	E	A	N	A	F	Q	D	A	A	A	P	A	E	E	V	D	P	A	A	A	L	K	A	E	L	R	S	K	P	G	D	W	Y	V	V	H	S	Y	A	G	Y	E	N
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		K	V	K	A	N	L	E	T	R	V	Q	N	L	D	V	G	D	Y	I	F	Q	V	E	V	P	T	E	E	V	T	E	I	K	N	G	Q	R	K	Q	V	N	R	K	V	L	P	G	Y	I	L	V	R	M	D	L	T	D	D	S	W
Secondary structure
SS confidence
Disorder													?	?	?	?																				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	A	V	R	N	T	P	G	V	T	G	F	V	G	A	T	S	R	P	S	A	L	A	L	D	D	V	V	K	F	L	L	P	R	G	S	T	R	K	A	A	K	G	A	A	S	T	A	A	A	A	E	A	G	G	L	E	R	P	V
Secondary structure
SS confidence
Disorder																																		?	?	?	?	?	?	?	?	?						?	?		?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.
Sequence		V	E	V	D	Y	E	V	G	E	S	V	T	V	M	D	G	P	F	A	T	L	P	A	T	I	S	E	V	N	A	E	Q	Q	K	L	K	V	L	V	S	I	F	G	R	E	T	P	V	E	L	T	F	G	Q	V	S	K	I
Secondary structure
SS confidence
Disorder		?																																									?		?										?		?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 32%) Alpha helix ( 25%) Beta strand ( 33%)

Rank Aligned region

PDB 1m1g chain B Region: 43 - 238 Aligned: 176 Modelled: 176 Confidence: 100.0% Identity: 39% PDB header:transcription Chain: B: PDB Molecule:transcription antitermination protein nusg; PDBTitle: crystal structure of aquifex aeolicus n-utilization2 substance g (nusg), space group p2(1) Phyre2 PDB 2xhc chain A Region: 47 - 238 Aligned: 170 Modelled: 170 Confidence: 100.0% Identity: 49% PDB header:transcription Chain: A: PDB Molecule:transcription antitermination protein nusg; PDBTitle: crystal structure of thermotoga maritima n-utilization substance g2 (nusg) Phyre2 PDB 2lq8 chain A Region: 44 - 238 Aligned: 175 Modelled: 182 Confidence: 100.0% Identity: 34% PDB header:transcription Chain: A: PDB Molecule:transcription antitermination protein nusg; PDBTitle: domain interaction in thermotoga maritima nusg Phyre2 PDB 5tbz chain K Region: 48 - 237 Aligned: 145 Modelled: 145 Confidence: 100.0% Identity: 43% PDB header:transcription/rna Chain: K: PDB Molecule:transcription termination/antitermination protein nusg; PDBTitle: e. coli rna polymerase complexed with nusg Phyre2 PDB 4zn3 chain A Region: 49 - 238 Aligned: 141 Modelled: 148 Confidence: 100.0% Identity: 21% PDB header:transcription Chain: A: PDB Molecule:transcription elongation factor spt5; PDBTitle: crystal structure of mjspt4:spt5 complex conformation b Phyre2 PDB 2oug chain C Region: 47 - 231 Aligned: 140 Modelled: 150 Confidence: 100.0% Identity: 23% PDB header:transcription Chain: C: PDB Molecule:transcriptional activator rfah; PDBTitle: crystal structure of the rfah transcription factor at 2.1a resolution Phyre2 PDB 3p8b chain B Region: 46 - 238 Aligned: 144 Modelled: 151 Confidence: 100.0% Identity: 23% PDB header:transferase/transcription Chain: B: PDB Molecule:transcription antitermination protein nusg; PDBTitle: x-ray crystal structure of pyrococcus furiosus transcription2 elongation factor spt4/5 Phyre2 PDB 2k06 chain A Region: 44 - 156 Aligned: 113 Modelled: 113 Confidence: 100.0% Identity: 37% PDB header:transcription Chain: A: PDB Molecule:transcription antitermination protein nusg; PDBTitle: solution structure of the aminoterminal domain of e. coli nusg Phyre2 PDB 1nz8 chain A Region: 48 - 155 Aligned: 108 Modelled: 108 Confidence: 100.0% Identity: 49% Fold: Ferredoxin-like Superfamily: N-utilization substance G protein NusG, N-terminal domain Family: N-utilization substance G protein NusG, N-terminal domain Phyre2 PDB 1m1h chain A domain 2 Region: 41 - 156 Aligned: 101 Modelled: 101 Confidence: 100.0% Identity: 28% Fold: Ferredoxin-like Superfamily: N-utilization substance G protein NusG, N-terminal domain Family: N-utilization substance G protein NusG, N-terminal domain Phyre2 PDB 5oik chain Z Region: 48 - 219 Aligned: 127 Modelled: 130 Confidence: 99.9% Identity: 17% PDB header:transcription Chain: Z: PDB Molecule:transcription elongation factor spt5; PDBTitle: structure of an rna polymerase ii-dsif transcription elongation2 complex Phyre2 PDB 5xon chain W Region: 48 - 219 Aligned: 125 Modelled: 129 Confidence: 99.8% Identity: 18% PDB header:transcription/rna Chain: W: PDB Molecule:protein that forms a complex with spt4p; PDBTitle: rna polymerase ii elongation complex bound with spt4/5 and tfiis Phyre2 PDB 2mi6 chain A Region: 177 - 238 Aligned: 62 Modelled: 62 Confidence: 99.8% Identity: 98% PDB header:transcription Chain: A: PDB Molecule:transcription termination/antitermination protein nusg; PDBTitle: solution structure of the carboxy terminal domain of nusg from2 mycobacterium tuberculosis Phyre2 PDB 1npp chain A domain 2 Region: 181 - 238 Aligned: 58 Modelled: 58 Confidence: 99.8% Identity: 52% Fold: SH3-like barrel Superfamily: Translation proteins SH3-like domain Family: N-utilization substance G protein NusG, C-terminal domain Phyre2 PDB 1nz9 chain A Region: 181 - 237 Aligned: 57 Modelled: 57 Confidence: 99.8% Identity: 53% Fold: SH3-like barrel Superfamily: Translation proteins SH3-like domain Family: N-utilization substance G protein NusG, C-terminal domain Phyre2 PDB 2jvv chain A Region: 180 - 237 Aligned: 58 Modelled: 58 Confidence: 99.7% Identity: 53% PDB header:transcription Chain: A: PDB Molecule:transcription antitermination protein nusg; PDBTitle: solution structure of e. coli nusg carboxyterminal domain Phyre2 PDB 2kvq chain G Region: 180 - 237 Aligned: 58 Modelled: 58 Confidence: 99.7% Identity: 53% PDB header:transcription Chain: G: PDB Molecule:transcription antitermination protein nusg; PDBTitle: solution structure of nuse:nusg-ctd complex Phyre2 PDB 2lcl chain A Region: 173 - 238 Aligned: 65 Modelled: 66 Confidence: 99.6% Identity: 20% PDB header:transcription Chain: A: PDB Molecule:transcriptional activator rfah; PDBTitle: solution structure of rfah carboxyterminal domain Phyre2 PDB 6ir9 chain W Region: 50 - 219 Aligned: 120 Modelled: 124 Confidence: 99.5% Identity: 19% PDB header:transcription/rna/dna Chain: W: PDB Molecule:spt5; PDBTitle: rna polymerase ii elongation complex bound with elf1 and spt4/5,2 stalled at shl(-1) of the nucleosome Phyre2 PDB 3ewg chain A Region: 50 - 151 Aligned: 78 Modelled: 85 Confidence: 98.4% Identity: 23% PDB header:transcription Chain: A: PDB Molecule:putative transcription antitermination protein nusg; PDBTitle: crystal structure of the n-terminal domain of nusg (ngn) from2 methanocaldococcus jannaschii Phyre2 1 c1m1gB_ 2 c2xhcA_ 3 c2lq8A_ 4 c5tbzK_ 5 c4zn3A_ 6 c2ougC_ 7 c3p8bB_ 8 c2k06A_ 9 d1nz8a_ 10 d1m1ha2 11 c5oikZ_ 12 c5xonW_ 13 c2mi6A_ 14 d1nppa2 15 d1nz9a_ 16 c2jvvA_ 17 c2kvqG_ 18 c2lclA_ 19 c6ir9W_ 20 c3ewgA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132