1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	L	S	A	F	I	S	S	L	R	T	V	D	L	R	R	K	I	L	F	T	L	G	I	V	I	L	Y	R	V	G	A	A	L	P	S	P	G	V	N	F	P	N	V	Q	Q	C	I	K	E	A	S	A	G	E	A	G	Q	I	Y
Secondary structure
SS confidence
Disorder		?	?																																																	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	L	I	N	L	F	S	G	G	A	L	L	K	L	T	V	F	A	V	G	V	M	P	Y	I	T	A	S	I	I	V	Q	L	L	T	V	V	I	P	R	F	E	E	L	R	K	E	G	Q	A	G	Q	S	K	M	T	Q	Y	T	R
Secondary structure
SS confidence
Disorder																				?	?	?																	?	?	?				?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	L	A	I	A	L	A	I	L	Q	A	T	S	I	V	A	L	A	A	N	G	G	L	L	Q	G	C	S	L	D	I	I	A	D	Q	S	I	F	T	L	V	V	I	V	L	V	M	T	G	G	A	A	L	V	M	W	M	G	E	L
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		I	T	E	R	G	I	G	N	G	M	S	L	L	I	F	V	G	I	A	A	R	I	P	A	E	G	Q	S	I	L	E	S	R	G	G	V	V	F	T	A	V	C	A	A	A	L	I	I	I	V	G	V	V	F	V	E	Q	G	Q	R
Secondary structure
SS confidence
Disorder																																				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		R	I	P	V	Q	Y	A	K	R	M	V	G	R	R	M	Y	G	G	T	S	T	Y	L	P	L	K	V	N	Q	A	G	V	I	P	V	I	F	A	S	S	L	I	Y	I	P	H	L	I	T	Q	L	I	R	S	G	S	G	V	V	G
Secondary structure
SS confidence
Disorder								?	?	?	?	?	?	?	?	?	?	?	?	?	?	?											?	?																				?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		N	S	W	W	D	K	F	V	G	T	Y	L	S	D	P	S	N	L	V	Y	I	G	I	Y	F	G	L	I	I	F	F	T	Y	F	Y	V	S	I	T	F	N	P	D	E	R	A	D	E	M	K	K	F	G	G	F	I	P	G	I	R
Secondary structure
SS confidence
Disorder		?	?																																						?	?											?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		P	G	R	P	T	A	D	Y	L	R	Y	V	L	S	R	I	T	L	P	G	S	I	Y	L	G	V	I	A	V	L	P	N	L	F	L	Q	I	G	A	G	G	T	V	Q	N	L	P	F	G	G	T	A	V	L	I	M	I	G	V	G
Secondary structure
SS confidence
Disorder																																									?	?	?	?	?	?	?	?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.
Sequence		L	D	T	V	K	Q	I	E	S	Q	L	M	Q	R	N	Y	E	G	F	L	K
Secondary structure
SS confidence
Disorder																?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 20%) Alpha helix ( 73%) Beta strand ( 1%) TM helix ( 45%) (View membrane topology prediction)

Rank Aligned region

PDB 3j01 chain A Region: 2 - 441 Aligned: 421 Modelled: 440 Confidence: 100.0% Identity: 41% PDB header:ribosome/ribosomal protein Chain: A: PDB Molecule:preprotein translocase secy subunit; PDBTitle: structure of the ribosome-secye complex in the membrane environment Phyre2 PDB 3din chain F Region: 8 - 441 Aligned: 395 Modelled: 415 Confidence: 100.0% Identity: 43% PDB header:membrane protein, protein transport Chain: F: PDB Molecule:preprotein translocase subunit secy; PDBTitle: crystal structure of the protein-translocation complex formed by the2 secy channel and the seca atpase Phyre2 PDB 3dl8 chain H Region: 9 - 441 Aligned: 399 Modelled: 399 Confidence: 100.0% Identity: 43% PDB header:protein transport Chain: H: PDB Molecule:preprotein translocase subunit secy; PDBTitle: structure of the complex of aquifex aeolicus secyeg and bacillus2 subtilis seca Phyre2 PDB 2zqp chain Y Region: 1 - 434 Aligned: 410 Modelled: 425 Confidence: 100.0% Identity: 41% PDB header:protein transport Chain: Y: PDB Molecule:preprotein translocase secy subunit; PDBTitle: crystal structure of secye translocon from thermus2 thermophilus Phyre2 PDB 5eul chain Y Region: 13 - 441 Aligned: 379 Modelled: 396 Confidence: 100.0% Identity: 45% PDB header:protein transport Chain: Y: PDB Molecule:protein translocase subunit secy; PDBTitle: structure of the seca-secy complex with a translocating polypeptide2 substrate Phyre2 PDB 2wwb chain A Region: 1 - 431 Aligned: 387 Modelled: 409 Confidence: 100.0% Identity: 21% PDB header:ribosome Chain: A: PDB Molecule:protein transport protein sec61 subunit alpha isoform 1; PDBTitle: cryo-em structure of the mammalian sec61 complex bound to the actively2 translating wheat germ 80s ribosome Phyre2 PDB 6n3q chain A Region: 1 - 430 Aligned: 346 Modelled: 346 Confidence: 100.0% Identity: 23% PDB header:transport protein Chain: A: PDB Molecule:protein transport protein sec61; PDBTitle: cryo-em structure of the yeast sec complex Phyre2 PDB 2aki chain Y Region: 12 - 441 Aligned: 377 Modelled: 390 Confidence: 100.0% Identity: 44% PDB header:protein transport Chain: Y: PDB Molecule:preprotein translocase secy subunit; PDBTitle: normal mode-based flexible fitted coordinates of a translocating2 secyeg protein-conducting channel into the cryo-em map of a secyeg-3 nascent chain-70s ribosome complex from e. coli Phyre2 PDB 2wwa chain A Region: 1 - 441 Aligned: 398 Modelled: 441 Confidence: 100.0% Identity: 20% PDB header:ribosome Chain: A: PDB Molecule:sec sixty-one protein homolog; PDBTitle: cryo-em structure of idle yeast ssh1 complex bound to the yeast 80s2 ribosome Phyre2 PDB 1rh5 chain A Region: 2 - 431 Aligned: 373 Modelled: 417 Confidence: 100.0% Identity: 23% Fold: Preprotein translocase SecY subunit Superfamily: Preprotein translocase SecY subunit Family: Preprotein translocase SecY subunit Phyre2 PDB 1vx1 chain 1 Region: 11 - 431 Aligned: 334 Modelled: 345 Confidence: 100.0% Identity: 23% PDB header:ribosome Chain: 1: PDB Molecule:sec61 alpha subunit; PDBTitle: structure of the translating mammalian ribosome-sec61 complex. this2 entry contains the large subunit ribosomal proteins. Phyre2 PDB 3mp7 chain A Region: 14 - 441 Aligned: 356 Modelled: 366 Confidence: 100.0% Identity: 24% PDB header:protein transport Chain: A: PDB Molecule:preprotein translocase subunit secy; PDBTitle: lateral opening of a translocon upon entry of protein suggests the2 mechanism of insertion into membranes Phyre2 PDB 2nsc chain A Region: 338 - 373 Aligned: 36 Modelled: 36 Confidence: 74.3% Identity: 33% PDB header:chaperone Chain: A: PDB Molecule:trigger factor; PDBTitle: structures of and interactions between domains of trigger factor from2 themotoga maritima Phyre2 PDB 1p9y chain A Region: 338 - 375 Aligned: 38 Modelled: 38 Confidence: 70.8% Identity: 21% Fold: Ribosome binding domain-like Superfamily: Trigger factor ribosome-binding domain Family: Trigger factor ribosome-binding domain Phyre2 PDB 1t11 chain A domain 2 Region: 344 - 375 Aligned: 32 Modelled: 32 Confidence: 61.3% Identity: 9% Fold: Ribosome binding domain-like Superfamily: Trigger factor ribosome-binding domain Family: Trigger factor ribosome-binding domain Phyre2 PDB 1w26 chain A domain 2 Region: 343 - 373 Aligned: 30 Modelled: 31 Confidence: 55.1% Identity: 17% Fold: Ribosome binding domain-like Superfamily: Trigger factor ribosome-binding domain Family: Trigger factor ribosome-binding domain Phyre2 PDB 2d3o chain 1 Region: 343 - 372 Aligned: 29 Modelled: 30 Confidence: 49.2% Identity: 21% PDB header:ribosome Chain: 1: PDB Molecule:trigger factor; PDBTitle: structure of ribosome binding domain of the trigger factor on the 50s2 ribosomal subunit from d. radiodurans Phyre2 PDB 1w26 chain B Region: 343 - 363 Aligned: 20 Modelled: 21 Confidence: 44.7% Identity: 25% PDB header:chaperone Chain: B: PDB Molecule:trigger factor; PDBTitle: trigger factor in complex with the ribosome forms a2 molecular cradle for nascent proteins Phyre2 PDB 1t11 chain A Region: 343 - 387 Aligned: 43 Modelled: 45 Confidence: 29.9% Identity: 12% PDB header:chaperone Chain: A: PDB Molecule:trigger factor; PDBTitle: trigger factor Phyre2 PDB 1wpg chain A domain 4 Region: 340 - 441 Aligned: 99 Modelled: 102 Confidence: 27.9% Identity: 12% Fold: Calcium ATPase, transmembrane domain M Superfamily: Calcium ATPase, transmembrane domain M Family: Calcium ATPase, transmembrane domain M Phyre2 1 c3j01A_ 2 c3dinF_ 3 c3dl8H_ 4 c2zqpY_ 5 c5eulY_ 6 c2wwbA_ 7 c6n3qA_ 8 c2akiY_ 9 c2wwaA_ 10 d1rh5a_ 11 c1vx11_ 12 c3mp7A_ 13 c2nscA_ 14 d1p9ya_ 15 d1t11a2 16 d1w26a2 17 c2d3o1_ 18 c1w26B_ 19 c1t11A_ 20 d1wpga4 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58