1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	D	P	L	M	A	H	Q	R	A	Q	D	A	F	A	A	L	L	A	N	V	R	A	D	Q	L	G	G	P	T	P	C	S	E	W	T	I	N	D	L	I	E	H	V	V	G	G	N	E	Q	V	G	R	W	A	A	S	P	I	E
Secondary structure
SS confidence
Disorder		?	?																																																								?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	P	A	R	P	D	G	L	V	A	A	H	Q	A	A	A	A	V	A	H	E	I	F	A	A	P	G	G	M	S	A	T	F	K	L	P	L	G	E	V	P	G	Q	V	F	I	G	L	R	T	T	D	V	L	T	H	A	W	D	L
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	A	A	T	G	Q	S	T	D	L	D	P	E	L	A	V	E	R	L	A	A	A	R	A	L	V	G	P	Q	F	R	G	P	G	K	P	F	A	D	E	K	P	C	P	R	E	R	P	P	A	D	Q	L	A	A	F	L	G	R	T
Secondary structure
SS confidence
Disorder																													?	?	?	?	?																											?	?
Disorder confidence
		.	.
Sequence		V	R
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 9%) Alpha helix ( 59%) Beta strand ( 0%)

Rank Aligned region

PDB 2nsf chain A Region: 2 - 181 Aligned: 180 Modelled: 180 Confidence: 100.0% Identity: 19% PDB header:isomerase Chain: A: PDB Molecule:hypothetical protein cgl3021; PDBTitle: crystal structure of the mycothiol-dependent maleylpyruvate isomerase Phyre2 PDB 2nsf chain A domain 1 Region: 2 - 127 Aligned: 126 Modelled: 125 Confidence: 100.0% Identity: 21% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: Maleylpyruvate isomerase-like Phyre2 PDB 2rd9 chain C Region: 4 - 139 Aligned: 136 Modelled: 136 Confidence: 98.6% Identity: 13% PDB header:hydrolase Chain: C: PDB Molecule:bh0186 protein; PDBTitle: crystal structure of a putative yfit-like metal-dependent hydrolase2 (bh0186) from bacillus halodurans c-125 at 2.30 a resolution Phyre2 PDB 5cog chain B Region: 2 - 55 Aligned: 54 Modelled: 54 Confidence: 98.4% Identity: 9% PDB header:unknown function Chain: B: PDB Molecule:irc4; PDBTitle: crystal structure of yeast irc4 Phyre2 PDB 6iz2 chain A Region: 2 - 129 Aligned: 128 Modelled: 128 Confidence: 98.4% Identity: 10% PDB header:unknown function Chain: A: PDB Molecule:dinb/yfit family protein; PDBTitle: crystal structure of dinb/yfit protein dr0053 from d. radiodurans r1 Phyre2 PDB 1rxq chain A Region: 4 - 122 Aligned: 119 Modelled: 119 Confidence: 98.2% Identity: 14% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: YfiT-like putative metal-dependent hydrolases Phyre2 PDB 5civ chain A Region: 4 - 88 Aligned: 85 Modelled: 85 Confidence: 98.2% Identity: 13% PDB header:unknown function Chain: A: PDB Molecule:sibling bacteriocin; PDBTitle: sibling lethal factor precursor - dfsb Phyre2 PDB 2ou6 chain A domain 1 Region: 4 - 121 Aligned: 118 Modelled: 118 Confidence: 98.0% Identity: 19% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 3cex chain B Region: 4 - 117 Aligned: 114 Modelled: 111 Confidence: 97.9% Identity: 18% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the conserved protein of locus ef_3021 from2 enterococcus faecalis Phyre2 PDB 4n6c chain B Region: 2 - 88 Aligned: 87 Modelled: 87 Confidence: 97.9% Identity: 10% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the b1rzq2 protein from streptococcus pneumoniae.2 northeast structural genomics consortium (nesg) target spr36. Phyre2 PDB 2yqy chain B Region: 4 - 124 Aligned: 117 Modelled: 120 Confidence: 97.9% Identity: 22% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein ttha0303; PDBTitle: crystal structure of tt2238, a four-helix bundle protein Phyre2 PDB 3di5 chain A Region: 2 - 129 Aligned: 128 Modelled: 128 Confidence: 97.8% Identity: 13% PDB header:metal binding protein Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (bce_4655) from bacillus2 cereus atcc 10987 at 2.01 a resolution Phyre2 PDB 3dka chain A Region: 2 - 132 Aligned: 131 Modelled: 131 Confidence: 97.8% Identity: 9% PDB header:unknown function Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (yjoa, bsu12410) from2 bacillus subtilis at 2.30 a resolution Phyre2 PDB 2p1a chain A domain 1 Region: 4 - 124 Aligned: 121 Modelled: 121 Confidence: 97.6% Identity: 10% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 2qe9 chain B Region: 7 - 132 Aligned: 126 Modelled: 126 Confidence: 97.4% Identity: 7% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein yiza; PDBTitle: crystal structure of a putative metal-dependent hydrolase (yiza,2 bsu10800) from bacillus subtilis at 1.90 a resolution Phyre2 PDB 3e4x chain B Region: 3 - 130 Aligned: 128 Modelled: 128 Confidence: 97.3% Identity: 9% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:apc36150; PDBTitle: crystal structure of putative metal-dependent hydrolases2 apc36150 Phyre2 PDB 5cof chain A Region: 4 - 111 Aligned: 108 Modelled: 108 Confidence: 97.2% Identity: 17% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterised protein q1r1x2 from escherichia2 coli uti89 Phyre2 PDB 5cqv chain B Region: 4 - 65 Aligned: 62 Modelled: 62 Confidence: 97.1% Identity: 8% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterized protein q8dwv2 from streptococcus2 agalactiae Phyre2 PDB 2hkv chain A domain 1 Region: 4 - 131 Aligned: 126 Modelled: 128 Confidence: 97.0% Identity: 13% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 6anr chain A Region: 4 - 65 Aligned: 62 Modelled: 62 Confidence: 96.9% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:colibactin self-protection protein clbs; PDBTitle: crystal structure of a self resistance protein clbs from colibactin2 biosynthetic gene cluster Phyre2 1 c2nsfA_ 2 d2nsfa1 3 c2rd9C_ 4 c5cogB_ 5 c6iz2A_ 6 d1rxqa_ 7 c5civA_ 8 d2ou6a1 9 c3cexB_ 10 c4n6cB_ 11 c2yqyB_ 12 c3di5A_ 13 c3dkaA_ 14 d2p1aa1 15 c2qe9B_ 16 c3e4xB_ 17 c5cofA_ 18 c5cqvB_ 19 d2hkva1 20 c6anrA_ 21 22 23 24 25 26 27 28 29