1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	R	L	I	G	R	L	R	L	L	M	V	G	L	V	V	I	C	G	A	C	A	C	D	R	V	S	A	G	R	W	S	E	S	P	S	A	T	S	W	P	V	R	P	V	N	T	T	T	P	S	G	P	V	P	P	V	S	E	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?		?																?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	R	A	A	G	L	V	D	V	R	G	V	V	P	D	A	A	I	D	L	R	Y	A	T	A	N	N	F	T	G	T	Q	L	Y	P	P	G	A	R	C	L	V	H	E	S	M	A	E	G	L	A	A	A	A	A	V	L	R	P	H
Secondary structure
SS confidence
Disorder		?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	Q	V	L	V	F	W	D	C	Y	R	P	H	D	V	Q	V	R	M	F	D	V	V	P	N	P	A	W	V	A	R	P	G	K	Y	A	H	S	H	E	A	G	R	S	V	D	V	T	F	A	S	A	Q	R	Q	C	P	S	V	R
Secondary structure
SS confidence
Disorder																										?	?	?		?		?	?	?	?	?		?	?																		?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		R	S	G	E	L	C	L	A	D	M	G	T	D	F	D	D	F	S	S	R	A	T	A	F	A	T	Q	G	V	S	A	E	A	Q	A	N	R	A	H	L	R	A	A	M	Q	A	G	G	L	T	V	Y	S	G	E	W	W	H	F	D
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.
Sequence		G	P	G	A	G	V	D	R	P	I	L	E	V	P	V	D
Secondary structure
SS confidence
Disorder																	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 25%) Alpha helix ( 25%) Beta strand ( 13%)

Rank Aligned region

PDB 1r44 chain A Region: 63 - 256 Aligned: 183 Modelled: 194 Confidence: 100.0% Identity: 29% Fold: Hedgehog/DD-peptidase Superfamily: Hedgehog/DD-peptidase Family: VanX-like Phyre2 PDB 4jid chain A Region: 72 - 243 Aligned: 138 Modelled: 141 Confidence: 99.7% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:d-alanyl-d-alanine carboxypeptidase family protein; PDBTitle: crystal structure of baldcb / vany-like l,d-carboxypeptidase zinc(ii)-2 free Phyre2 PDB 4ox3 chain A Region: 92 - 243 Aligned: 119 Modelled: 121 Confidence: 99.6% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:putative carboxypeptidase yodj; PDBTitle: structure of the ldcb ld-carboxypeptidase reveals the molecular basis2 of peptidoglycan recognition Phyre2 PDB 4mur chain A Region: 72 - 245 Aligned: 142 Modelled: 157 Confidence: 99.5% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:d,d-dipeptidase/d,d-carboxypeptidase; PDBTitle: crystal structure of vancomycin resistance d,d-dipeptidase/d,d-2 pentapeptidase vanxyc d59s mutant Phyre2 PDB 5hnm chain C Region: 76 - 243 Aligned: 128 Modelled: 131 Confidence: 99.4% Identity: 23% PDB header:hydrolase Chain: C: PDB Molecule:d-alanyl-d-alanine carboxypeptidase; PDBTitle: crystal structure of vancomycin resistance d,d-pentapeptidase vany2 e175a mutant from vanb-type resistance cassette in complex with3 zn(ii) Phyre2 PDB 2vo9 chain C Region: 102 - 245 Aligned: 113 Modelled: 113 Confidence: 99.4% Identity: 17% PDB header:hydrolase Chain: C: PDB Molecule:l-alanyl-d-glutamate peptidase; PDBTitle: crystal structure of the enzymatically active domain of the listeria2 monocytogenes bacteriophage 500 endolysin ply500 Phyre2 PDB 2vo9 chain A domain 1 Region: 102 - 254 Aligned: 115 Modelled: 133 Confidence: 99.4% Identity: 19% Fold: Hedgehog/DD-peptidase Superfamily: Hedgehog/DD-peptidase Family: VanY-like Phyre2 PDB 4ox5 chain A Region: 102 - 243 Aligned: 104 Modelled: 106 Confidence: 98.7% Identity: 30% PDB header:hydrolase Chain: A: PDB Molecule:ldcb ld-carboxypeptidase; PDBTitle: structure of the ldcb ld-carboxypeptidase reveals the molecular basis2 of peptidoglycan recognition Phyre2 PDB 4f78 chain A Region: 92 - 245 Aligned: 121 Modelled: 124 Confidence: 98.5% Identity: 25% PDB header:hydrolase Chain: A: PDB Molecule:d,d-dipeptidase/d,d-carboxypeptidase; PDBTitle: crystal structure of vancomycin resistance d,d-dipeptidase vanxyg Phyre2 PDB 2mxz chain A Region: 111 - 253 Aligned: 106 Modelled: 113 Confidence: 97.8% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:l-alanyl-d-glutamate peptidase; PDBTitle: bacteriophage t5 l-alanoyl-d-glutamate peptidase comlpex with zn2+2 (endo t5-zn2+) Phyre2 PDB 5opz chain B Region: 102 - 170 Aligned: 59 Modelled: 69 Confidence: 96.8% Identity: 25% PDB header:hydrolase Chain: B: PDB Molecule:chix; PDBTitle: crystal structure of serratia marcescens l-ala d-glu endopeptidase2 chix Phyre2 PDB 3d1m chain A domain 1 Region: 101 - 176 Aligned: 59 Modelled: 59 Confidence: 95.1% Identity: 22% Fold: Hedgehog/DD-peptidase Superfamily: Hedgehog/DD-peptidase Family: Hedgehog (development protein), N-terminal signaling domain Phyre2 PDB 3m1n chain B Region: 101 - 176 Aligned: 59 Modelled: 59 Confidence: 94.3% Identity: 22% PDB header:signaling protein Chain: B: PDB Molecule:sonic hedgehog protein; PDBTitle: crystal structure of human sonic hedgehog n-terminal domain Phyre2 PDB 2ibg chain E domain 1 Region: 101 - 176 Aligned: 59 Modelled: 59 Confidence: 90.5% Identity: 17% Fold: Hedgehog/DD-peptidase Superfamily: Hedgehog/DD-peptidase Family: Hedgehog (development protein), N-terminal signaling domain Phyre2 PDB 1lbu chain A Region: 102 - 167 Aligned: 54 Modelled: 66 Confidence: 72.9% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:muramoyl-pentapeptide carboxypeptidase; PDBTitle: hydrolase metallo (zn) dd-peptidase Phyre2 PDB 1eiy chain A Region: 217 - 249 Aligned: 33 Modelled: 33 Confidence: 58.8% Identity: 24% PDB header:ligase/rna Chain: A: PDB Molecule:phenylalanyl-trna synthetase; PDBTitle: the crystal structure of phenylalanyl-trna synthetase from thermus2 thermophilus complexed with cognate trnaphe Phyre2 PDB 4nz3 chain A Region: 103 - 132 Aligned: 30 Modelled: 30 Confidence: 56.6% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:deacetylase da1; PDBTitle: structure of vibrio cholerae chitin de-n-acetylase in complex with2 di(n-acetyl-d-glucosamine) (cbs) in p 21 21 21 Phyre2 PDB 3l4g chain I Region: 217 - 252 Aligned: 36 Modelled: 36 Confidence: 53.7% Identity: 25% PDB header:ligase Chain: I: PDB Molecule:phenylalanyl-trna synthetase alpha chain; PDBTitle: crystal structure of homo sapiens cytoplasmic phenylalanyl-trna2 synthetase Phyre2 PDB 1lbu chain A domain 2 Region: 102 - 167 Aligned: 54 Modelled: 66 Confidence: 51.6% Identity: 19% Fold: Hedgehog/DD-peptidase Superfamily: Hedgehog/DD-peptidase Family: Muramoyl-pentapeptide carboxypeptidase Phyre2 PDB 2k4z chain A Region: 86 - 127 Aligned: 42 Modelled: 42 Confidence: 50.8% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:dsrr; PDBTitle: solution nmr structure of allochromatium vinosum dsrr:2 northeast structural genomics consortium target op5 Phyre2 1 d1r44a_ 2 c4jidA_ 3 c4ox3A_ 4 c4murA_ 5 c5hnmC_ 6 c2vo9C_ 7 d2vo9a1 8 c4ox5A_ 9 c4f78A_ 10 c2mxzA_ 11 c5opzB_ 12 d3d1ma1 13 c3m1nB_ 14 d2ibge1 15 c1lbuA_ 16 c1eiyA_ 17 c4nz3A_ 18 c3l4gI_ 19 d1lbua2 20 c2k4zA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99