1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	R	E	T	S	N	P	V	F	R	S	L	P	K	Q	R	G	G	Y	A	Q	F	G	T	G	T	A	Q	Q	G	F	P	A	D	P	Y	L	A	P	Y	R	E	A	K	A	T	R	P	L	T	I	D	D	V	V	T	K	T	G	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	L	A	M	L	A	G	T	A	V	V	S	Y	F	L	V	A	S	N	V	A	L	A	M	P	L	T	L	V	G	A	L	G	G	L	A	L	V	L	V	A	T	F	G	R	K	Q	D	N	P	A	I	V	L	S	Y	A	A	L	E
Secondary structure
SS confidence
Disorder																			?	?	?																							?		?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	L	F	L	G	A	I	S	F	V	L	A	N	F	T	V	A	S	A	N	A	G	V	L	I	G	E	A	I	L	G	T	M	G	V	F	F	G	M	L	V	V	Y	K	T	G	A	I	R	V	T	P	K	F	T	R	M	V	V	A
Secondary structure
SS confidence
Disorder																					?	?																												?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	L	F	G	V	L	V	L	M	L	G	N	L	V	L	A	M	F	N	V	G	G	G	E	G	L	G	L	R	S	P	G	P	L	G	I	I	F	S	L	V	C	I	G	I	A	A	F	S	F	L	I	D	F	D	A	A	D	Q	M
Secondary structure
SS confidence
Disorder																								?	?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.
Sequence		I	R	A	G	A	P	E	K	A	A	W	G	V	A	L	G	L	T	V	T	L	V	W	L	Y	I	E	I	L	R	L	L	S	Y	L	Q	N	E
Secondary structure
SS confidence
Disorder				?	?	?																															?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 25%) Alpha helix ( 69%) Beta strand ( 0%) TM helix ( 56%) (View membrane topology prediction)

Rank Aligned region

PDB 4pgu chain A Region: 50 - 277 Aligned: 205 Modelled: 228 Confidence: 100.0% Identity: 20% PDB header:membrance protein Chain: A: PDB Molecule:uncharacterized protein yetj; PDBTitle: crystal structure of yetj from bacillus subtilis at ph 7 by soaking Phyre2 PDB 4pgw chain A Region: 54 - 275 Aligned: 194 Modelled: 204 Confidence: 100.0% Identity: 19% PDB header:membrance protein Chain: A: PDB Molecule:uncharacterized protein yetj; PDBTitle: crystal structure of yetj from bacillus subtilis at ph 6 by pt-sad Phyre2 PDB 5vyl chain A Region: 230 - 262 Aligned: 32 Modelled: 33 Confidence: 28.5% Identity: 6% PDB header:viral protein Chain: A: PDB Molecule:inner tegument protein; PDBTitle: crystal structure of n-terminal half of herpes simplex virus type 12 ul37 protein Phyre2 PDB 4k70 chain B Region: 230 - 262 Aligned: 32 Modelled: 33 Confidence: 20.3% Identity: 25% PDB header:viral protein Chain: B: PDB Molecule:ul37; PDBTitle: crystal structure of n-terminal half of pseudorabiesvirus ul37 protein Phyre2 PDB 4z6y chain D Region: 266 - 278 Aligned: 13 Modelled: 13 Confidence: 10.7% Identity: 38% PDB header:hydrolase inhibitor/protein binding Chain: D: PDB Molecule:hamartin; PDBTitle: structure of the tbc1d7-tsc1 complex Phyre2 PDB 4z6y chain C Region: 266 - 278 Aligned: 13 Modelled: 13 Confidence: 10.7% Identity: 38% PDB header:hydrolase inhibitor/protein binding Chain: C: PDB Molecule:hamartin; PDBTitle: structure of the tbc1d7-tsc1 complex Phyre2 PDB 5oqt chain C Region: 214 - 231 Aligned: 18 Modelled: 18 Confidence: 10.4% Identity: 6% PDB header:transport protein Chain: C: PDB Molecule:uncharacterized protein ynem; PDBTitle: crystal structure of a bacterial cationic amino acid transporter (cat)2 homologue Phyre2 PDB 4z6y chain F Region: 266 - 278 Aligned: 13 Modelled: 13 Confidence: 9.9% Identity: 38% PDB header:hydrolase inhibitor/protein binding Chain: F: PDB Molecule:hamartin; PDBTitle: structure of the tbc1d7-tsc1 complex Phyre2 PDB 6f34 chain C Region: 212 - 231 Aligned: 20 Modelled: 20 Confidence: 9.6% Identity: 10% PDB header:membrane protein Chain: C: PDB Molecule:mgts; PDBTitle: crystal structure of a bacterial cationic amino acid transporter (cat)2 homologue bound to arginine. Phyre2 PDB 6hwh chain W Region: 105 - 269 Aligned: 164 Modelled: 165 Confidence: 9.4% Identity: 12% PDB header:electron transport Chain: W: PDB Molecule:cytochrome c oxidase subunit 3; PDBTitle: structure of a functional obligate respiratory supercomplex from2 mycobacterium smegmatis Phyre2 PDB 1m47 chain A Region: 229 - 259 Aligned: 31 Modelled: 31 Confidence: 9.2% Identity: 13% Fold: 4-helical cytokines Superfamily: 4-helical cytokines Family: Short-chain cytokines Phyre2 PDB 5ejc chain C Region: 266 - 275 Aligned: 10 Modelled: 10 Confidence: 8.7% Identity: 50% PDB header:signaling protein Chain: C: PDB Molecule:hamartin; PDBTitle: crystal structural of the tsc1-tbc1d7 complex Phyre2 PDB 6ith chain A Region: 141 - 166 Aligned: 26 Modelled: 26 Confidence: 7.8% Identity: 31% PDB header:membrane protein Chain: A: PDB Molecule:syndecan-2; PDBTitle: structure of the transmembrane domain of syndecan 2 in micelles Phyre2 PDB 5z1l chain L Region: 141 - 161 Aligned: 21 Modelled: 21 Confidence: 6.4% Identity: 14% PDB header:protein fibril Chain: L: PDB Molecule:flagellin; PDBTitle: cryo-em structure of methanoccus maripaludis archaellum Phyre2 PDB 1v54 chain C Region: 113 - 269 Aligned: 155 Modelled: 157 Confidence: 5.6% Identity: 12% Fold: Cytochrome c oxidase subunit III-like Superfamily: Cytochrome c oxidase subunit III-like Family: Cytochrome c oxidase subunit III-like Phyre2 1 c4pguA_ 2 c4pgwA_ 3 c5vylA_ 4 c4k70B_ 5 c4z6yD_ 6 c4z6yC_ 7 c5oqtC_ 8 c4z6yF_ 9 c6f34C_ 10 c6hwhW_ 11 d1m47a_ 12 c5ejcC_ 13 c6ithA_ 14 c5z1lL_ 15 d1v54c_