1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	V	D	A	D	S	A	N	Y	R	I	E	H	D	T	M	G	E	V	R	V	P	A	K	A	L	W	R	A	Q	T	Q	R	A	V	E	N	F	P	I	S	G	R	G	L	E	R	T	Q	I	R	A	L	G	L	L	K	G	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?			?															?														?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		C	A	Q	V	N	S	D	L	G	L	L	A	P	E	K	A	D	A	I	I	A	A	A	A	E	I	A	D	G	Q	H	D	D	Q	F	P	I	D	V	F	Q	T	G	S	G	T	S	S	N	M	N	T	N	E	V	I	A	S	I	A
Secondary structure
SS confidence
Disorder																																															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	K	G	G	V	T	L	H	P	N	D	D	V	N	M	S	Q	S	S	N	D	T	F	P	T	A	T	H	I	A	A	T	E	A	A	V	A	H	L	I	P	A	L	Q	Q	L	H	D	A	L	A	A	K	A	L	D	W	H	T	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	K	S	G	R	T	H	L	M	D	A	V	P	V	T	L	G	Q	E	F	S	G	Y	A	R	Q	I	E	A	G	I	E	R	V	R	A	C	L	P	R	L	G	E	L	A	I	G	G	T	A	V	G	T	G	L	N	A	P	D	D
Secondary structure
SS confidence
Disorder																																										?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	G	V	R	V	V	A	V	L	V	A	Q	T	G	L	S	E	L	R	T	A	A	N	S	F	E	A	Q	A	A	R	D	G	L	V	E	A	S	G	A	L	R	T	I	A	V	S	L	T	K	I	A	N	D	I	R	W	M	G	S
Secondary structure
SS confidence
Disorder																				?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		G	P	L	T	G	L	A	E	I	Q	L	P	D	L	Q	P	G	S	S	I	M	P	G	K	V	N	P	V	L	P	E	A	V	T	Q	V	A	A	Q	V	I	G	N	D	A	A	I	A	W	G	G	A	N	G	A	F	E	L	N	V
Secondary structure
SS confidence
Disorder													?	?	?	?	?	?																																			?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		Y	I	P	M	M	A	R	N	I	L	E	S	F	K	L	L	T	N	V	S	R	L	F	A	Q	R	C	I	A	G	L	T	A	N	V	E	H	L	R	R	L	A	E	S	S	P	S	I	V	T	P	L	N	S	A	I	G	Y	E	E
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.
Sequence		A	A	A	V	A	K	Q	A	L	K	E	R	K	T	I	R	Q	T	V	I	D	R	G	L	I	G	D	R	L	S	I	E	D	L	D	R	R	L	D	V	L	A	M	A	K	A	E	Q	L	D	S	D	R	L
Secondary structure
SS confidence
Disorder																									?	?	?		?																	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 11%) Alpha helix ( 63%) Beta strand ( 1%)

Rank Aligned region

PDB 6n1m chain A Region: 8 - 467 Aligned: 455 Modelled: 460 Confidence: 100.0% Identity: 47% PDB header:lyase Chain: A: PDB Molecule:fumarate hydratase class ii; PDBTitle: crystal structure of fumarate hydratase class ii from legionella2 pneumophila philadelphia 1 Phyre2 PDB 3gtd chain A Region: 9 - 461 Aligned: 448 Modelled: 449 Confidence: 100.0% Identity: 49% PDB header:lyase Chain: A: PDB Molecule:fumarate hydratase class ii; PDBTitle: 2.4 angstrom crystal structure of fumarate hydratase from rickettsia2 prowazekii Phyre2 PDB 3no9 chain C Region: 7 - 466 Aligned: 453 Modelled: 454 Confidence: 100.0% Identity: 99% PDB header:lyase Chain: C: PDB Molecule:fumarate hydratase class ii; PDBTitle: crystal structure of apo fumarate hydratase from mycobacterium2 tuberculosis Phyre2 PDB 1yfm chain A Region: 10 - 464 Aligned: 447 Modelled: 451 Confidence: 100.0% Identity: 50% PDB header:lyase Chain: A: PDB Molecule:fumarase; PDBTitle: recombinant yeast fumarase Phyre2 PDB 1yfm chain A Region: 10 - 464 Aligned: 447 Modelled: 451 Confidence: 100.0% Identity: 50% Fold: L-aspartase-like Superfamily: L-aspartase-like Family: L-aspartase/fumarase Phyre2 PDB 3e04 chain A Region: 10 - 466 Aligned: 446 Modelled: 457 Confidence: 100.0% Identity: 51% PDB header:lyase Chain: A: PDB Molecule:fumarate hydratase; PDBTitle: crystal structure of human fumarate hydratase Phyre2 PDB 1vdk chain A Region: 11 - 467 Aligned: 450 Modelled: 453 Confidence: 100.0% Identity: 51% Fold: L-aspartase-like Superfamily: L-aspartase-like Family: L-aspartase/fumarase Phyre2 PDB 1j3u chain A Region: 11 - 470 Aligned: 454 Modelled: 460 Confidence: 100.0% Identity: 44% Fold: L-aspartase-like Superfamily: L-aspartase-like Family: L-aspartase/fumarase Phyre2 PDB 4hgv chain D Region: 10 - 466 Aligned: 446 Modelled: 457 Confidence: 100.0% Identity: 47% PDB header:lyase Chain: D: PDB Molecule:fumarate hydratase class ii; PDBTitle: crystal structure of a fumarate hydratase Phyre2 PDB 1jsw chain A Region: 7 - 459 Aligned: 447 Modelled: 453 Confidence: 100.0% Identity: 42% Fold: L-aspartase-like Superfamily: L-aspartase-like Family: L-aspartase/fumarase Phyre2 PDB 1fuo chain A Region: 11 - 463 Aligned: 448 Modelled: 453 Confidence: 100.0% Identity: 50% Fold: L-aspartase-like Superfamily: L-aspartase-like Family: L-aspartase/fumarase Phyre2 PDB 3ocf chain B Region: 10 - 441 Aligned: 420 Modelled: 432 Confidence: 100.0% Identity: 46% PDB header:lyase Chain: B: PDB Molecule:fumarate lyase:delta crystallin; PDBTitle: crystal structure of fumarate lyase:delta crystallin from brucella2 melitensis in native form Phyre2 PDB 3e04 chain C Region: 10 - 415 Aligned: 404 Modelled: 406 Confidence: 100.0% Identity: 53% PDB header:lyase Chain: C: PDB Molecule:fumarate hydratase; PDBTitle: crystal structure of human fumarate hydratase Phyre2 PDB 3gtd chain B Region: 9 - 406 Aligned: 393 Modelled: 398 Confidence: 100.0% Identity: 50% PDB header:lyase Chain: B: PDB Molecule:fumarate hydratase class ii; PDBTitle: 2.4 angstrom crystal structure of fumarate hydratase from rickettsia2 prowazekii Phyre2 PDB 4hgv chain C Region: 10 - 414 Aligned: 402 Modelled: 405 Confidence: 100.0% Identity: 48% PDB header:lyase Chain: C: PDB Molecule:fumarate hydratase class ii; PDBTitle: crystal structure of a fumarate hydratase Phyre2 PDB 1jsw chain C Region: 10 - 414 Aligned: 403 Modelled: 405 Confidence: 100.0% Identity: 43% Fold: L-aspartase-like Superfamily: L-aspartase-like Family: L-aspartase/fumarase Phyre2 PDB 3r6y chain G Region: 11 - 395 Aligned: 376 Modelled: 385 Confidence: 100.0% Identity: 46% PDB header:lyase Chain: G: PDB Molecule:aspartase; PDBTitle: crystal structure of chymotrypsin-treated aspartase from bacillus sp.2 ym55-1 Phyre2 PDB 5xnz chain A Region: 27 - 474 Aligned: 415 Modelled: 440 Confidence: 100.0% Identity: 23% PDB header:lyase Chain: A: PDB Molecule:cred; PDBTitle: crystal structure of cred complex with fumarate Phyre2 PDB 1re5 chain A Region: 25 - 474 Aligned: 415 Modelled: 436 Confidence: 100.0% Identity: 24% Fold: L-aspartase-like Superfamily: L-aspartase-like Family: L-aspartase/fumarase Phyre2 PDB 2pfm chain A Region: 27 - 474 Aligned: 412 Modelled: 441 Confidence: 100.0% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:adenylosuccinate lyase; PDBTitle: crystal structure of adenylosuccinate lyase (purb) from bacillus2 anthracis Phyre2 1 c6n1mA_ 2 c3gtdA_ 3 c3no9C_ 4 c1yfmA_ 5 d1yfma_ 6 c3e04A_ 7 d1vdka_ 8 d1j3ua_ 9 c4hgvD_ 10 d1jswa_ 11 d1fuoa_ 12 c3ocfB_ 13 c3e04C_ 14 c3gtdB_ 15 c4hgvC_ 16 d1jswc_ 17 c3r6yG_ 18 c5xnzA_ 19 d1re5a_ 20 c2pfmA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99