1

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10

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20

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30

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40

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50

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60

Sequence

M

R

T

T

V

T

V

D

D

A

L

L

A

K

A

A

E

L

T

G

V

K

E

K

S

T

L

L

R

E

G

L

Q

T

L

V

R

V

E

S

A

R

R

L

A

A

L

G

G

T

D

P

Q

A

T

A

A

P

R

R

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

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.

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Sequence

R

T

S

P

R

Secondary structure

SS confidence

Disorder

?

?

?

?

?

Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 48%) Alpha helix ( 57%) Beta strand ( 5%)

Rank Aligned region

PDB 6a7v chain U Region: 2 - 60 Aligned: 59 Modelled: 59 Confidence: 99.7% Identity: 27% PDB header:toxin/antitoxin Chain: U: PDB Molecule:antitoxin vapb11; PDBTitle: crystal structure of mycobacterium tuberculosis vapbc11 toxin-2 antitoxin complex Phyre2 PDB 2bj3 chain D Region: 1 - 42 Aligned: 42 Modelled: 42 Confidence: 95.4% Identity: 17% PDB header:transcription Chain: D: PDB Molecule:nickel responsive regulator; PDBTitle: nikr-apo Phyre2 PDB 2bj7 chain A domain 1 Region: 1 - 40 Aligned: 40 Modelled: 40 Confidence: 94.9% Identity: 18% Fold: Ribbon-helix-helix Superfamily: Ribbon-helix-helix Family: CopG-like Phyre2 PDB 2ca9 chain B Region: 1 - 42 Aligned: 42 Modelled: 42 Confidence: 94.6% Identity: 21% PDB header:transcription Chain: B: PDB Molecule:putative nickel-responsive regulator; PDBTitle: apo-nikr from helicobacter pylori in closed trans-2 conformation Phyre2 PDB 2hza chain A domain 1 Region: 2 - 41 Aligned: 40 Modelled: 40 Confidence: 93.9% Identity: 33% Fold: Ribbon-helix-helix Superfamily: Ribbon-helix-helix Family: CopG-like Phyre2 PDB 1q5v chain B Region: 1 - 36 Aligned: 36 Modelled: 36 Confidence: 93.3% Identity: 33% PDB header:transcription Chain: B: PDB Molecule:nickel responsive regulator; PDBTitle: apo-nikr Phyre2 PDB 2hza chain B domain 1 Region: 2 - 36 Aligned: 35 Modelled: 35 Confidence: 87.3% Identity: 34% Fold: Ribbon-helix-helix Superfamily: Ribbon-helix-helix Family: CopG-like Phyre2 PDB 5ceg chain C Region: 1 - 43 Aligned: 43 Modelled: 43 Confidence: 82.5% Identity: 19% PDB header:toxin Chain: C: PDB Molecule:addiction module antidote protein, copg/arc/metj family; PDBTitle: x-ray structure of toxin/anti-toxin complex from mesorhizobium2 opportunistum Phyre2 PDB 2k5j chain B Region: 1 - 50 Aligned: 50 Modelled: 50 Confidence: 77.6% Identity: 16% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein yiif; PDBTitle: solution structure of protein yiif from shigella flexneri2 serotype 5b (strain 8401) . northeast structural genomics3 consortium target sft1 Phyre2 PDB 3kxe chain D Region: 3 - 46 Aligned: 44 Modelled: 44 Confidence: 68.8% Identity: 20% PDB header:protein binding Chain: D: PDB Molecule:antitoxin protein pard-1; PDBTitle: a conserved mode of protein recognition and binding in a2 pard-pare toxin-antitoxin complex Phyre2 PDB 4me7 chain E Region: 2 - 46 Aligned: 45 Modelled: 43 Confidence: 67.1% Identity: 11% PDB header:hydrolase/hydrolase inhibitor Chain: E: PDB Molecule:antitoxin endoai; PDBTitle: crystal structure of bacillus subtilis toxin mazf in complex with2 cognate antitoxin maze Phyre2 PDB 6iya chain D Region: 3 - 42 Aligned: 40 Modelled: 40 Confidence: 40.5% Identity: 20% PDB header:antitoxin Chain: D: PDB Molecule:transcriptional regulator copg family; PDBTitle: structure of the dna binding domain of antitoxin copaso Phyre2 PDB 5vgt chain A Region: 21 - 35 Aligned: 15 Modelled: 15 Confidence: 40.4% Identity: 20% PDB header:viral protein Chain: A: PDB Molecule:gene 7 protein; PDBTitle: x-ray structure of bacteriophage sf6 tail adaptor protein gp7 Phyre2 PDB 2rbf chain B Region: 3 - 39 Aligned: 37 Modelled: 37 Confidence: 21.0% Identity: 19% PDB header:oxidoreductase/dna Chain: B: PDB Molecule:bifunctional protein puta; PDBTitle: structure of the ribbon-helix-helix domain of escherichia coli puta2 (puta52) complexed with operator dna (o2) Phyre2 PDB 2kel chain B Region: 5 - 39 Aligned: 35 Modelled: 35 Confidence: 20.7% Identity: 11% PDB header:transcription repressor Chain: B: PDB Molecule:uncharacterized protein 56b; PDBTitle: structure of the transcription regulator svtr from the2 hyperthermophilic archaeal virus sirv1 Phyre2 PDB 3fmt chain F Region: 4 - 13 Aligned: 10 Modelled: 10 Confidence: 19.7% Identity: 50% PDB header:replication inhibitor/dna Chain: F: PDB Molecule:protein seqa; PDBTitle: crystal structure of seqa bound to dna Phyre2 PDB 1y66 chain D Region: 26 - 40 Aligned: 15 Modelled: 15 Confidence: 19.0% Identity: 20% PDB header:de novo protein Chain: D: PDB Molecule:engrailed homeodomain; PDBTitle: dioxane contributes to the altered conformation and2 oligomerization state of a designed engrailed homeodomain3 variant Phyre2 PDB 1xrx chain D Region: 4 - 13 Aligned: 10 Modelled: 10 Confidence: 18.5% Identity: 50% PDB header:replication inhibitor Chain: D: PDB Molecule:seqa protein; PDBTitle: crystal structure of a dna-binding protein Phyre2 PDB 1xrx chain A domain 1 Region: 4 - 13 Aligned: 10 Modelled: 10 Confidence: 18.5% Identity: 50% Fold: Ribbon-helix-helix Superfamily: Ribbon-helix-helix Family: SeqA N-terminal domain-like Phyre2 PDB 2zq5 chain A Region: 2 - 37 Aligned: 36 Modelled: 36 Confidence: 16.3% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of sulfotransferase stf1 from2 mycobacterium tuberculosis h37rv (type1 form) Phyre2 1 c6a7vU_ 2 c2bj3D_ 3 d2bj7a1 4 c2ca9B_ 5 d2hzaa1 6 c1q5vB_ 7 d2hzab1 8 c5cegC_ 9 c2k5jB_ 10 c3kxeD_ 11 c4me7E_ 12 c6iyaD_ 13 c5vgtA_ 14 c2rbfB_ 15 c2kelB_ 16 c3fmtF_ 17 c1y66D_ 18 c1xrxD_ 19 d1xrxa1 20 c2zq5A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39