1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	A	G	H	R	M	A	A	V	D	A	Q	F	Y	W	M	S	A	K	V	P	N	D	Q	F	L	L	Y	A	F	D	G	E	P	T	D	L	E	R	A	V	A	Q	V	Y	R	R	A	R	G	C	P	G	L	G	M	R	V	Q	D
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	G	A	L	A	Y	P	Q	W	V	P	T	P	V	Q	R	D	Q	L	V	C	H	D	L	A	D	R	S	W	Q	G	C	L	A	A	V	V	G	L	A	S	K	Q	L	D	M	R	R	M	P	W	R	L	H	V	F	T	P	V	H
Secondary structure
SS confidence
Disorder		?																																													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		D	V	P	G	V	S	G	L	G	T	V	A	V	M	Q	F	A	H	A	L	G	D	G	A	R	A	S	A	M	A	A	W	L	F	G	R	P	A	A	V	P	E	I	A	R	S	R	A	G	F	L	P	W	R	A	A	H	A	A	R
Secondary structure
SS confidence
Disorder			?																																					?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	H	L	R	L	V	R	D	T	N	A	G	L	V	A	P	G	V	G	S	R	P	P	L	S	T	N	A	R	P	E	G	V	R	A	V	R	T	L	L	R	R	R	S	Q	L	A	G	P	T	V	T	V	T	V	L	A	A	V	S
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		T	G	L	L	G	L	L	G	G	D	V	D	T	L	G	A	E	V	P	M	A	K	P	G	V	P	R	S	Y	N	H	F	G	N	V	V	V	G	L	Y	P	R	L	E	P	D	E	R	V	R	R	I	A	T	D	L	A	N	A	R
Secondary structure
SS confidence
Disorder												?											?	?	?	?	?	?	?									?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	R	F	E	H	P	A	M	L	S	A	D	R	A	F	A	A	V	P	A	A	L	L	R	W	G	V	S	Q	F	D	A	E	V	R	P	V	R	V	A	G	N	T	V	V	S	S	V	Y	R	G	A	A	D	L	S	F	G	D	A
Secondary structure
SS confidence
Disorder		?	?	?	?																										?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.
Sequence		P	V	V	L	T	A	G	Y	P	A	L	S	P	A	M	G	L	T	H	G	V	H	G	I	G	D	T	V	A	I	S	V	H	A	A	E	S	A	V	S	D	I	D	A	Y	M	R	L	L	D	A	A	L	Q
Secondary structure
SS confidence
Disorder													?																																									?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 20%) Alpha helix ( 39%) Beta strand ( 21%) TM helix ( 4%) (View membrane topology prediction)

Rank Aligned region

PDB 6chj chain B Region: 3 - 414 Aligned: 392 Modelled: 412 Confidence: 100.0% Identity: 17% PDB header:transferase Chain: B: PDB Molecule:diacylglycerol o-acyltransferase; PDBTitle: wax ester synthase/diacylglycerol acyltransferase from marinobacter2 aquaeolei vt8 Phyre2 PDB 1q9j chain A Region: 4 - 414 Aligned: 362 Modelled: 380 Confidence: 100.0% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:polyketide synthase associated protein 5; PDBTitle: structure of polyketide synthase associated protein 5 from2 mycobacterium tuberculosis Phyre2 PDB 2jgp chain A Region: 5 - 414 Aligned: 379 Modelled: 395 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: structure of the tycc5-6 pcp-c bidomain of the tyrocidine synthetase2 tycc Phyre2 PDB 5m6p chain B Region: 5 - 414 Aligned: 385 Modelled: 396 Confidence: 100.0% Identity: 9% PDB header:isomerase Chain: B: PDB Molecule:tyrocidine synthase 2; PDBTitle: crystal structure of the epimerization domain from module 3 of2 tyrocidine synthetase b, tycb3(e) Phyre2 PDB 5t3e chain A Region: 5 - 414 Aligned: 383 Modelled: 395 Confidence: 100.0% Identity: 10% PDB header:ligase Chain: A: PDB Molecule:bacillamide synthetase heterocyclization domain; PDBTitle: crystal structure of a nonribosomal peptide synthetase2 heterocyclization domain. Phyre2 PDB 5u89 chain A Region: 5 - 414 Aligned: 371 Modelled: 387 Confidence: 100.0% Identity: 14% PDB header:hydrolase/inhibitor Chain: A: PDB Molecule:amino acid adenylation domain protein; PDBTitle: crystal structure of a cross-module fragment from the dimodular nrps2 dhbf Phyre2 PDB 6aef chain B Region: 5 - 413 Aligned: 379 Modelled: 390 Confidence: 100.0% Identity: 12% PDB header:transferase Chain: B: PDB Molecule:polyketide synthase associated protein papa2; PDBTitle: papa2 acyl transferase Phyre2 PDB 5t81 chain A Region: 5 - 414 Aligned: 383 Modelled: 395 Confidence: 100.0% Identity: 15% PDB header:biosynthetic protein Chain: A: PDB Molecule:epob; PDBTitle: rhombohedral crystal form of the epob nrps cyclization-docking2 bidomain from sorangium cellulosum Phyre2 PDB 6ad3 chain A Region: 5 - 414 Aligned: 390 Modelled: 407 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: A: PDB Molecule:lovastatin nonaketide synthase moka; PDBTitle: structural characterization of the condensation domain from monacolin2 k polyketide synthase moka Phyre2 PDB 4zxi chain A Region: 5 - 414 Aligned: 383 Modelled: 393 Confidence: 100.0% Identity: 11% PDB header:biosynthetic protein Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: crystal structure of holo-ab3403 a four domain nonribosomal peptide2 synthetase bound to amp and glycine Phyre2 PDB 6m7l chain B Region: 5 - 414 Aligned: 384 Modelled: 410 Confidence: 100.0% Identity: 15% PDB header:biosynthetic protein Chain: B: PDB Molecule:putative non-ribosomal peptide synthetase; PDBTitle: complex of oxya with the x-domain from gpa biosynthesis Phyre2 PDB 4znm chain B Region: 5 - 414 Aligned: 382 Modelled: 394 Confidence: 100.0% Identity: 16% PDB header:ligase Chain: B: PDB Molecule:c-domain type ii peptide synthetase; PDBTitle: crystal structure of sgcc5 protein from streptomyces globisporus (apo2 form) Phyre2 PDB 6cgo chain B Region: 5 - 414 Aligned: 385 Modelled: 407 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: B: PDB Molecule:condensation domain protein; PDBTitle: molecular basis for condensation domain-mediated chain release from2 the enacyloxin polyketide synthase Phyre2 PDB 2xhg chain A Region: 5 - 414 Aligned: 385 Modelled: 396 Confidence: 100.0% Identity: 11% PDB header:isomerase Chain: A: PDB Molecule:tyrocidine synthetase a; PDBTitle: crystal structure of the epimerization domain from the initiation2 module of tyrocidine biosynthesis Phyre2 PDB 2vsq chain A Region: 5 - 414 Aligned: 381 Modelled: 391 Confidence: 100.0% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 4jn3 chain B Region: 5 - 414 Aligned: 377 Modelled: 386 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: B: PDB Molecule:cda peptide synthetase i; PDBTitle: crystal structures of the first condensation domain of the cda2 synthetase Phyre2 PDB 1l5a chain A Region: 6 - 414 Aligned: 373 Modelled: 388 Confidence: 100.0% Identity: 9% PDB header:biosynthetic protein Chain: A: PDB Molecule:amide synthase; PDBTitle: crystal structure of vibh, an nrps condensation enzyme Phyre2 PDB 6p1j chain A Region: 5 - 414 Aligned: 378 Modelled: 393 Confidence: 100.0% Identity: 18% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 5ja2 chain A Region: 17 - 414 Aligned: 363 Modelled: 379 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 4tx3 chain B Region: 5 - 414 Aligned: 382 Modelled: 382 Confidence: 100.0% Identity: 15% PDB header:oxidoreductase Chain: B: PDB Molecule:peptide synthetase, module 7; PDBTitle: complex of the x-domain and oxyb from teicoplanin biosynthesis Phyre2 1 c6chjB_ 2 c1q9jA_ 3 c2jgpA_ 4 c5m6pB_ 5 c5t3eA_ 6 c5u89A_ 7 c6aefB_ 8 c5t81A_ 9 c6ad3A_ 10 c4zxiA_ 11 c6m7lB_ 12 c4znmB_ 13 c6cgoB_ 14 c2xhgA_ 15 c2vsqA_ 16 c4jn3B_ 17 c1l5aA_ 18 c6p1jA_ 19 c5ja2A_ 20 c4tx3B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64