1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	R	I	T	R	A	N	G	C	P	D	G	T	L	E	N	A	V	V	A	L	D	G	G	A	N	Y	P	R	E	I	L	G	N	K	G	H	G	I	D	M	M	R	R	H	H	L	P	V	P	P	A	F	C	I	T	T	E	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?												?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	V	R	Y	L	A	A	P	G	S	T	I	A	A	I	W	D	D	V	L	D	R	M	S	W	L	E	T	E	T	S	C	T	F	G	R	G	P	N	P	L	L	V	S	V	R	S	G	A	T	Q	S	M	P	G	M	M	D	T	I
Secondary structure
SS confidence
Disorder											?		?																																			?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	D	V	G	M	T	D	A	V	E	R	V	L	A	R	P	G	A	A	D	F	A	H	D	T	R	R	R	F	T	S	M	Y	R	R	I	V	G	S	A	G	P	I	T	D	D	P	Y	A	Q	L	R	A	S	I	E	A	V	F	A
Secondary structure
SS confidence
Disorder																						?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	W	N	S	P	R	A	V	A	Y	R	D	H	H	G	L	D	D	Q	G	G	T	A	V	V	V	Q	A	M	V	F	G	N	L	T	A	N	S	G	A	G	V	L	S	S	R	N	P	I	T	G	A	N	E	P	F	G	E	W	L
Secondary structure
SS confidence
Disorder															?		?															?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		P	G	G	Q	G	D	D	V	V	S	G	L	V	A	V	A	P	I	T	A	L	R	D	Q	Q	P	A	V	Y	D	Q	L	M	A	A	A	R	S	L	E	R	M	A	G	D	V	Q	E	I	E	F	T	V	E	D	S	Q	L	W	L
Secondary structure
SS confidence
Disorder											?																																?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	Q	T	R	G	A	E	R	S	A	Q	A	A	V	R	L	A	L	Q	L	H	H	E	G	L	I	D	D	T	E	T	L	R	R	V	T	P	T	H	I	E	T	L	L	R	P	S	L	Q	T	E	T	R	L	A	A	P	L	L	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		K	G	L	P	A	C	P	G	V	V	S	G	T	A	Y	T	E	V	D	E	A	L	D	A	A	D	R	G	E	P	V	I	L	V	R	D	H	T	R	P	E	D	V	M	G	M	L	A	A	Q	G	I	V	T	E	V	G	G	A	A
Secondary structure
SS confidence
Disorder								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		S	H	A	A	V	V	S	R	E	L	G	R	V	A	V	V	G	C	G	P	G	V	A	A	A	L	A	G	K	E	I	T	V	D	G	Y	E	G	E	V	R	Q	G	V	L	A	L	S	A	W	S	E	S	D	T	P	E	L	R	E
Secondary structure
SS confidence
Disorder		?																																												?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490
Sequence		L	A	D	I	A	Q	R	I	S	S
Secondary structure
SS confidence
Disorder										?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 11%) Alpha helix ( 47%) Beta strand ( 14%)

Rank Aligned region

PDB 1kbl chain A Region: 17 - 490 Aligned: 469 Modelled: 474 Confidence: 100.0% Identity: 39% PDB header:transferase Chain: A: PDB Molecule:pyruvate phosphate dikinase; PDBTitle: pyruvate phosphate dikinase Phyre2 PDB 1h6z chain A Region: 16 - 490 Aligned: 470 Modelled: 475 Confidence: 100.0% Identity: 37% PDB header:transferase Chain: A: PDB Molecule:pyruvate phosphate dikinase; PDBTitle: 3.0 a resolution crystal structure of glycosomal pyruvate2 phosphate dikinase from trypanosoma brucei Phyre2 PDB 1vbh chain A Region: 21 - 490 Aligned: 456 Modelled: 470 Confidence: 100.0% Identity: 38% PDB header:transferase Chain: A: PDB Molecule:pyruvate,orthophosphate dikinase; PDBTitle: pyruvate phosphate dikinase with bound mg-pep from maize Phyre2 PDB 2ols chain A Region: 17 - 469 Aligned: 356 Modelled: 384 Confidence: 100.0% Identity: 28% PDB header:transferase Chain: A: PDB Molecule:phosphoenolpyruvate synthase; PDBTitle: the crystal structure of the phosphoenolpyruvate synthase from2 neisseria meningitidis Phyre2 PDB 5fbt chain A Region: 18 - 460 Aligned: 351 Modelled: 375 Confidence: 100.0% Identity: 32% PDB header:transferase/antibiotic Chain: A: PDB Molecule:phosphoenolpyruvate synthase; PDBTitle: crystal structure of rifampin phosphotransferase rph-lm from listeria2 monocytogenes in complex with rifampin Phyre2 PDB 1kbl chain A domain 3 Region: 17 - 343 Aligned: 325 Modelled: 327 Confidence: 100.0% Identity: 40% Fold: ATP-grasp Superfamily: Glutathione synthetase ATP-binding domain-like Family: Pyruvate phosphate dikinase, N-terminal domain Phyre2 PDB 1vbg chain A domain 3 Region: 16 - 346 Aligned: 329 Modelled: 331 Confidence: 100.0% Identity: 41% Fold: ATP-grasp Superfamily: Glutathione synthetase ATP-binding domain-like Family: Pyruvate phosphate dikinase, N-terminal domain Phyre2 PDB 1h6z chain A domain 3 Region: 16 - 345 Aligned: 326 Modelled: 330 Confidence: 100.0% Identity: 37% Fold: ATP-grasp Superfamily: Glutathione synthetase ATP-binding domain-like Family: Pyruvate phosphate dikinase, N-terminal domain Phyre2 PDB 1kbl chain A domain 2 Region: 344 - 464 Aligned: 121 Modelled: 121 Confidence: 100.0% Identity: 44% Fold: The "swivelling" beta/beta/alpha domain Superfamily: Phosphohistidine domain Family: Pyruvate phosphate dikinase, central domain Phyre2 PDB 1vbg chain A domain 2 Region: 347 - 469 Aligned: 123 Modelled: 123 Confidence: 100.0% Identity: 40% Fold: The "swivelling" beta/beta/alpha domain Superfamily: Phosphohistidine domain Family: Pyruvate phosphate dikinase, central domain Phyre2 PDB 1h6z chain A domain 2 Region: 346 - 467 Aligned: 122 Modelled: 122 Confidence: 100.0% Identity: 41% Fold: The "swivelling" beta/beta/alpha domain Superfamily: Phosphohistidine domain Family: Pyruvate phosphate dikinase, central domain Phyre2 PDB 1zym chain A domain 2 Region: 361 - 466 Aligned: 106 Modelled: 106 Confidence: 99.9% Identity: 25% Fold: The "swivelling" beta/beta/alpha domain Superfamily: Phosphohistidine domain Family: N-terminal domain of enzyme I of the PEP:sugar phosphotransferase system Phyre2 PDB 3t07 chain D Region: 356 - 466 Aligned: 111 Modelled: 111 Confidence: 99.9% Identity: 23% PDB header:transferase/transferase inhibitor Chain: D: PDB Molecule:pyruvate kinase; PDBTitle: crystal structure of s. aureus pyruvate kinase in complex with a2 naturally occurring bis-indole alkaloid Phyre2 PDB 2e28 chain A Region: 356 - 468 Aligned: 113 Modelled: 113 Confidence: 99.9% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:pyruvate kinase; PDBTitle: crystal structure analysis of pyruvate kinase from bacillus2 stearothermophilus Phyre2 PDB 5woy chain A Region: 383 - 483 Aligned: 95 Modelled: 101 Confidence: 99.9% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:phosphoenolpyruvate-protein phosphotransferase; PDBTitle: nmr solution structure of enzyme i (neit) protein using two 4d-spectra Phyre2 PDB 2hwg chain A Region: 383 - 489 Aligned: 104 Modelled: 107 Confidence: 99.9% Identity: 19% PDB header:transferase Chain: A: PDB Molecule:phosphoenolpyruvate-protein phosphotransferase; PDBTitle: structure of phosphorylated enzyme i of the phosphoenolpyruvate:sugar2 phosphotransferase system Phyre2 PDB 1eza chain A Region: 379 - 487 Aligned: 103 Modelled: 109 Confidence: 99.9% Identity: 21% PDB header:phosphotransferase Chain: A: PDB Molecule:enzyme i; PDBTitle: amino terminal domain of enzyme i from escherichia coli nmr,2 restrained regularized mean structure Phyre2 PDB 5t1o chain B Region: 386 - 490 Aligned: 98 Modelled: 105 Confidence: 99.8% Identity: 29% PDB header:transferase Chain: B: PDB Molecule:phosphoenolpyruvate-protein phosphotransferase ptsp; PDBTitle: solution-state nmr and saxs structural ensemble of npr (1-85) in2 complex with ein-ntr (170-424) Phyre2 PDB 2hro chain A Region: 386 - 487 Aligned: 96 Modelled: 102 Confidence: 99.4% Identity: 25% PDB header:transferase Chain: A: PDB Molecule:phosphoenolpyruvate-protein phosphotransferase; PDBTitle: structure of the full-lenght enzyme i of the pts system from2 staphylococcus carnosus Phyre2 PDB 2hi6 chain A domain 1 Region: 360 - 461 Aligned: 99 Modelled: 102 Confidence: 96.4% Identity: 17% Fold: The "swivelling" beta/beta/alpha domain Superfamily: LeuD/IlvD-like Family: AF0055-like Phyre2 1 c1kblA_ 2 c1h6zA_ 3 c1vbhA_ 4 c2olsA_ 5 c5fbtA_ 6 d1kbla3 7 d1vbga3 8 d1h6za3 9 d1kbla2 10 d1vbga2 11 d1h6za2 12 d1zyma2 13 c3t07D_ 14 c2e28A_ 15 c5woyA_ 16 c2hwgA_ 17 c1ezaA_ 18 c5t1oB_ 19 c2hroA_ 20 d2hi6a1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105