1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	P	D	Q	D	T	K	V	R	F	F	R	V	F	C	W	C	P	V	L	R	M	V	R	I	M	L	M	H	A	V	R	A	W	R	S	A	D	D	F	P	C	T	E	H	M	A	Y	K	I	A	Q	V	A	A	D	P	V	D	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																				?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	P	E	V	A	D	M	V	C	N	R	I	I	D	N	A	A	V	S	A	A	S	M	V	R	R	P	V	T	V	A	R	H	Q	A	L	A	H	P	V	R	H	G	A	K	V	F	G	V	E	G	S	Y	S	A	D	W	A	A	W
Secondary structure
SS confidence
Disorder																																						?														?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	N	G	V	A	A	R	E	L	D	F	H	D	T	F	L	A	A	D	Y	S	H	P	A	D	N	I	P	P	L	V	A	V	A	Q	Q	L	G	V	C	G	A	E	L	I	R	G	L	V	T	A	Y	E	I	H	I	D	L	T	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	I	C	L	H	E	H	K	I	D	H	V	A	H	L	G	P	A	V	A	A	G	I	G	T	M	L	R	L	D	Q	E	T	I	Y	H	A	I	G	Q	A	L	H	L	T	T	S	T	R	Q	S	R	K	G	A	I	S	S	W	K
Secondary structure
SS confidence
Disorder																																																						?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	F	A	P	A	H	A	G	K	V	G	I	E	A	V	D	R	A	M	R	G	E	G	S	P	A	P	I	W	E	G	E	D	G	V	I	A	W	L	L	A	G	P	E	H	T	Y	R	V	P	L	P	A	P	G	E	P	K	R	A
Secondary structure
SS confidence
Disorder																										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		I	L	D	S	Y	T	K	Q	H	S	A	E	Y	Q	S	Q	A	P	I	D	L	A	C	R	L	R	E	R	I	G	D	L	D	Q	I	A	S	I	V	L	H	T	S	H	H	T	H	V	V	I	G	T	G	S	G	D	P	Q	K	F
Secondary structure
SS confidence
Disorder																													?			?																										?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		D	P	D	A	S	R	E	T	L	D	H	S	L	P	Y	I	F	A	V	A	L	Q	D	G	C	W	H	H	E	R	S	Y	A	P	E	R	A	R	R	S	D	T	V	A	L	W	H	K	I	S	T	V	E	D	P	E	W	T	R	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		Y	H	C	A	D	P	A	K	K	A	F	G	A	R	A	E	V	T	L	H	S	G	E	V	I	V	D	E	L	A	V	A	D	A	H	P	L	G	T	R	P	F	E	R	K	Q	Y	V	E	K	F	T	E	L	A	D	G	V	V	E
Secondary structure
SS confidence
Disorder				?	?	?	?	?	?	?	?																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.
Sequence		P	V	E	Q	Q	R	F	L	A	V	V	E	S	L	A	D	L	E	S	G	A	V	G	G	L	N	V	L	V	D	P	R	V	L	D	K	A	P	V	I	P	P	G	I	F	R
Secondary structure
SS confidence
Disorder																					?	?													?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 12%) Alpha helix ( 52%) Beta strand ( 7%)

Rank Aligned region

PDB 2hp0 chain A Region: 41 - 505 Aligned: 439 Modelled: 464 Confidence: 100.0% Identity: 18% PDB header:isomerase Chain: A: PDB Molecule:ids-epimerase; PDBTitle: crystal structure of iminodisuccinate epimerase Phyre2 PDB 5mux chain B Region: 40 - 510 Aligned: 456 Modelled: 471 Confidence: 100.0% Identity: 21% PDB header:lyase Chain: B: PDB Molecule:2-methylcitrate dehydratase; PDBTitle: crystal structure of 2-methylcitrate dehydratase (mmge) from bacillus2 subtilis. Phyre2 PDB 1szq chain A Region: 44 - 510 Aligned: 452 Modelled: 467 Confidence: 100.0% Identity: 23% Fold: 2-methylcitrate dehydratase PrpD Superfamily: 2-methylcitrate dehydratase PrpD Family: 2-methylcitrate dehydratase PrpD Phyre2 PDB 4rqo chain B Region: 143 - 262 Aligned: 117 Modelled: 118 Confidence: 97.5% Identity: 18% PDB header:lyase Chain: B: PDB Molecule:l-serine dehydratase; PDBTitle: crystal structure of l-serine dehydratase from legionella pneumophila Phyre2 PDB 1i27 chain A Region: 459 - 495 Aligned: 37 Modelled: 37 Confidence: 41.9% Identity: 16% Fold: DNA/RNA-binding 3-helical bundle Superfamily: "Winged helix" DNA-binding domain Family: C-terminal domain of the rap74 subunit of TFIIF Phyre2 PDB 5gao chain N Region: 429 - 455 Aligned: 27 Modelled: 27 Confidence: 40.9% Identity: 19% PDB header:transcription Chain: N: PDB Molecule: PDBTitle: head region of the yeast spliceosomal u4/u6.u5 tri-snrnp Phyre2 PDB 3jb9 chain D Region: 429 - 455 Aligned: 27 Modelled: 27 Confidence: 40.3% Identity: 19% PDB header:rna binding protein/rna Chain: D: PDB Molecule:small nuclear ribonucleoprotein sm d3; PDBTitle: cryo-em structure of the yeast spliceosome at 3.6 angstrom resolution Phyre2 PDB 3cw1 chain D Region: 429 - 455 Aligned: 27 Modelled: 27 Confidence: 39.2% Identity: 26% PDB header:splicing Chain: D: PDB Molecule:small nuclear ribonucleoprotein sm d3; PDBTitle: crystal structure of human spliceosomal u1 snrnp Phyre2 PDB 1b34 chain A Region: 428 - 455 Aligned: 28 Modelled: 28 Confidence: 37.7% Identity: 14% PDB header:rna binding protein Chain: A: PDB Molecule:protein (small nuclear ribonucleoprotein sm d1); PDBTitle: crystal structure of the d1d2 sub-complex from the human snrnp core2 domain Phyre2 PDB 1b34 chain A Region: 428 - 455 Aligned: 28 Modelled: 28 Confidence: 37.7% Identity: 14% Fold: Sm-like fold Superfamily: Sm-like ribonucleoproteins Family: Sm motif of small nuclear ribonucleoproteins, SNRNP Phyre2 PDB 1d3b chain A Region: 429 - 455 Aligned: 27 Modelled: 27 Confidence: 33.9% Identity: 26% Fold: Sm-like fold Superfamily: Sm-like ribonucleoproteins Family: Sm motif of small nuclear ribonucleoproteins, SNRNP Phyre2 PDB 4emh chain Y Region: 432 - 455 Aligned: 24 Modelled: 24 Confidence: 33.7% Identity: 29% PDB header:rna binding protein Chain: Y: PDB Molecule: PDBTitle: crystal structure of splsm4 Phyre2 PDB 6an0 chain A Region: 110 - 173 Aligned: 64 Modelled: 64 Confidence: 33.5% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:histidinol dehydrogenase; PDBTitle: crystal structure of histidinol dehydrogenase from elizabethkingia2 anophelis Phyre2 PDB 4c92 chain D Region: 429 - 455 Aligned: 27 Modelled: 27 Confidence: 32.3% Identity: 30% PDB header:transcription Chain: D: PDB Molecule:u6 snrna-associated sm-like protein lsm4; PDBTitle: crystal structure of the yeast lsm1-7 complex Phyre2 PDB 3e21 chain A Region: 462 - 508 Aligned: 40 Modelled: 47 Confidence: 32.2% Identity: 13% PDB header:apoptosis Chain: A: PDB Molecule:fas-associated factor 1; PDBTitle: crystal structure of faf-1 uba domain Phyre2 PDB 2fwk chain B Region: 429 - 455 Aligned: 27 Modelled: 27 Confidence: 31.3% Identity: 22% PDB header:dna binding protein Chain: B: PDB Molecule:u6 snrna-associated sm-like protein lsm5; PDBTitle: crystal structure of cryptosporidium parvum u6 snrna-associated sm-2 like protein lsm5 Phyre2 PDB 3jcr chain 3 Region: 429 - 449 Aligned: 21 Modelled: 21 Confidence: 30.9% Identity: 14% PDB header:splicing Chain: 3: PDB Molecule:lsm3; PDBTitle: 3d structure determination of the human*u4/u6.u5* tri-snrnp complex Phyre2 PDB 5mkn chain K Region: 429 - 455 Aligned: 27 Modelled: 27 Confidence: 30.7% Identity: 26% PDB header:rna binding protein Chain: K: PDB Molecule:like-sm ribonucleoprotein core; PDBTitle: crystal structure of smap (lsm) protein from methanococcus vannielii Phyre2 PDB 3jb9 chain B Region: 434 - 455 Aligned: 22 Modelled: 22 Confidence: 30.5% Identity: 27% PDB header:rna binding protein/rna Chain: B: PDB Molecule:pre-mrna-splicing factor cwf10; PDBTitle: cryo-em structure of the yeast spliceosome at 3.6 angstrom resolution Phyre2 PDB 5gan chain R Region: 415 - 455 Aligned: 31 Modelled: 41 Confidence: 30.2% Identity: 19% PDB header:transcription Chain: R: PDB Molecule: PDBTitle: the overall structure of the yeast spliceosomal u4/u6.u5 tri-snrnp at2 3.7 angstrom Phyre2 1 c2hp0A_ 2 c5muxB_ 3 d1szqa_ 4 c4rqoB_ 5 d1i27a_ 6 c5gaon_ 7 c3jb9D_ 8 c3cw1D_ 9 c1b34A_ 10 d1b34a_ 11 d1d3ba_ 12 c4emhY_ 13 c6an0A_ 14 c4c92D_ 15 c3e21A_ 16 c2fwkB_ 17 c3jcr3_ 18 c5mknK_ 19 c3jb9b_ 20 c5ganr_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99