1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	Q	R	I	A	R	L	A	I	A	A	P	R	R	I	I	G	F	A	V	F	V	F	I	A	A	A	V	F	G	V	P	V	A	D	S	L	S	P	G	G	F	Q	D	P	R	S	E	S	A	R	A	I	E	V	L	T	D	K
Secondary structure
SS confidence
Disorder		?																															?	?	?	?	?	?	?	?	?	?	?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	G	Q	S	G	Q	K	M	L	I	V	V	T	A	A	A	G	A	D	S	P	P	A	R	E	V	G	T	D	I	V	E	V	L	R	R	S	P	L	V	Y	N	V	T	S	P	W	T	V	P	P	T	A	A	A	D	L	L	S	T
Secondary structure
SS confidence
Disorder																?			?	?	?																													?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		D	G	K	S	G	L	I	V	V	N	V	K	G	G	E	N	D	A	Q	N	H	A	Q	T	L	S	D	E	V	A	H	D	R	D	G	V	T	V	R	A	G	G	S	A	M	E	Y	A	Q	I	N	R	Q	N	K	D	D	L	L	V
Secondary structure
SS confidence
Disorder														?	?	?	?	?																																							?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		M	E	L	I	A	I	P	L	S	F	L	V	L	I	W	V	F	G	G	L	L	A	A	G	L	P	M	A	Q	A	V	L	A	V	V	G	S	M	A	V	L	R	L	V	T	F	A	T	E	V	S	T	F	A	L	N	L	S	T	A
Secondary structure
SS confidence
Disorder																																																			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	G	L	A	L	A	I	D	Y	T	L	L	I	V	S	R	Y	R	D	E	L	A	E	G	S	D	R	D	E	A	L	I	R	T	M	A	L	R	G	A	R	C	C	F	R	R	S	P	W	R	C	R	C	R	R	L	R	C	S	R
Secondary structure
SS confidence
Disorder																								?	?	?	?																																?	?	?
Disorder confidence
		.	.	.
Sequence		C	T	F
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 16%) Alpha helix ( 67%) Beta strand ( 7%) TM helix ( 32%) (View membrane topology prediction)

Rank Aligned region

PDB 6ajj chain A Region: 1 - 303 Aligned: 303 Modelled: 303 Confidence: 100.0% Identity: 24% PDB header:membrane protein, hydrolase Chain: A: PDB Molecule:drug exporters of the rnd superfamily-like protein, PDBTitle: crystal structure of mycolic acid transporter mmpl3 from mycobacterium2 smegmatis complexed with ica38 Phyre2 PDB 5khn chain B Region: 1 - 301 Aligned: 298 Modelled: 301 Confidence: 100.0% Identity: 14% PDB header:membrane protein Chain: B: PDB Molecule:rnd transporter; PDBTitle: crystal structures of the burkholderia multivorans hopanoid2 transporter hpnn Phyre2 PDB 3jd8 chain A Region: 2 - 292 Aligned: 289 Modelled: 289 Confidence: 100.0% Identity: 17% PDB header:membrane protein Chain: A: PDB Molecule:niemann-pick c1 protein; PDBTitle: cryo-em structure of the full-length human npc1 at 4.4 angstrom Phyre2 PDB 6dmo chain A Region: 11 - 300 Aligned: 282 Modelled: 290 Confidence: 100.0% Identity: 13% PDB header:protein binding Chain: A: PDB Molecule:protein patched homolog 1; PDBTitle: cryo-em structure of human ptch1 with three mutations2 l282q/t500f/p504l Phyre2 PDB 6ows chain B Region: 2 - 302 Aligned: 294 Modelled: 297 Confidence: 100.0% Identity: 17% PDB header:membrane protein Chain: B: PDB Molecule:efflux pump membrane transporter; PDBTitle: cryo-em structure of an acinetobacter baumannii multidrug efflux pump Phyre2 PDB 6csx chain A Region: 5 - 299 Aligned: 289 Modelled: 295 Confidence: 100.0% Identity: 16% PDB header:transport protein Chain: A: PDB Molecule:multidrug efflux pump subunit acrb; PDBTitle: single particles cryo-em structure of acrb d407a associated with lipid2 bilayer at 3.0 angstrom Phyre2 PDB 5lq3 chain F Region: 2 - 302 Aligned: 294 Modelled: 301 Confidence: 100.0% Identity: 17% PDB header:transport protein Chain: F: PDB Molecule:cmeb; PDBTitle: structures and transport dynamics of the campylobacter jejuni2 multidrug efflux pump cmeb Phyre2 PDB 2v50 chain A Region: 2 - 299 Aligned: 291 Modelled: 298 Confidence: 100.0% Identity: 16% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein mexb; PDBTitle: the missing part of the bacterial mexab-oprm system: structural2 determination of the multidrug exporter mexb Phyre2 PDB 4mt1 chain A Region: 6 - 302 Aligned: 291 Modelled: 297 Confidence: 100.0% Identity: 16% PDB header:membrane protein, tranport protein Chain: A: PDB Molecule:drug efflux protein; PDBTitle: crystal structure of the neisseria gonorrhoeae mtrd inner membrane2 multidrug efflux pump Phyre2 PDB 3k07 chain A Region: 5 - 300 Aligned: 290 Modelled: 296 Confidence: 100.0% Identity: 17% PDB header:transport protein Chain: A: PDB Molecule:cation efflux system protein cusa; PDBTitle: crystal structure of cusa Phyre2 PDB 1oy8 chain A Region: 2 - 301 Aligned: 284 Modelled: 287 Confidence: 99.9% Identity: 19% PDB header:membrane protein Chain: A: PDB Molecule:acriflavine resistance protein b; PDBTitle: structural basis of multiple drug binding capacity of the acrb2 multidrug efflux pump Phyre2 PDB 4k0e chain C Region: 4 - 299 Aligned: 282 Modelled: 296 Confidence: 99.9% Identity: 12% PDB header:transport protein Chain: C: PDB Molecule:heavy metal cation tricomponent efflux pump znea(czca- PDBTitle: x-ray crystal structure of a heavy metal efflux pump, crystal form ii Phyre2 PDB 3aqp chain B Region: 8 - 299 Aligned: 239 Modelled: 249 Confidence: 99.9% Identity: 11% PDB header:membrane protein Chain: B: PDB Molecule:probable secdf protein-export membrane protein; PDBTitle: crystal structure of secdf, a translocon-associated membrane protein,2 from thermus thrmophilus Phyre2 PDB 5xam chain A Region: 11 - 296 Aligned: 273 Modelled: 286 Confidence: 99.9% Identity: 16% PDB header:membrane protein Chain: A: PDB Molecule:protein translocase subunit secd; PDBTitle: crystal structure of secdf in i form at 4 a resolution Phyre2 PDB 4k0e chain A Region: 4 - 302 Aligned: 285 Modelled: 299 Confidence: 99.9% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:heavy metal cation tricomponent efflux pump znea(czca- PDBTitle: x-ray crystal structure of a heavy metal efflux pump, crystal form ii Phyre2 PDB 5mg3 chain F Region: 10 - 296 Aligned: 241 Modelled: 252 Confidence: 99.8% Identity: 10% PDB header:chaperone Chain: F: PDB Molecule:protein translocase subunit secf; PDBTitle: em fitted model of bacterial holo-translocon Phyre2 PDB 1iwg chain A domain 7 Region: 135 - 302 Aligned: 162 Modelled: 168 Confidence: 99.8% Identity: 15% Fold: Multidrug efflux transporter AcrB transmembrane domain Superfamily: Multidrug efflux transporter AcrB transmembrane domain Family: Multidrug efflux transporter AcrB transmembrane domain Phyre2 PDB 1iwg chain A domain 8 Region: 135 - 292 Aligned: 152 Modelled: 158 Confidence: 99.7% Identity: 14% Fold: Multidrug efflux transporter AcrB transmembrane domain Superfamily: Multidrug efflux transporter AcrB transmembrane domain Family: Multidrug efflux transporter AcrB transmembrane domain Phyre2 PDB 5mg3 chain D Region: 80 - 296 Aligned: 208 Modelled: 217 Confidence: 99.7% Identity: 13% PDB header:chaperone Chain: D: PDB Molecule:protein translocase subunit secd; PDBTitle: em fitted model of bacterial holo-translocon Phyre2 PDB 4f0a chain B Region: 247 - 293 Aligned: 47 Modelled: 47 Confidence: 46.3% Identity: 17% PDB header:signaling protein Chain: B: PDB Molecule:protein wnt-8; PDBTitle: crystal structure of xwnt8 in complex with the cysteine-rich domain of2 frizzled 8 Phyre2 1 c6ajjA_ 2 c5khnB_ 3 c3jd8A_ 4 c6dmoA_ 5 c6owsB_ 6 c6csxA_ 7 c5lq3F_ 8 c2v50A_ 9 c4mt1A_ 10 c3k07A_ 11 c1oy8A_ 12 c4k0eC_ 13 c3aqpB_ 14 c5xamA_ 15 c4k0eA_ 16 c5mg3F_ 17 d1iwga7 18 d1iwga8 19 c5mg3D_ 20 c4f0aB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74