1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	L	L	A	S	L	N	P	A	V	V	S	A	A	D	I	A	D	A	V	R	I	D	G	D	V	L	S	R	S	D	L	V	G	A	A	T	S	V	A	E	R	V	A	G	A	H	R	V	A	V	L	A	T	P	T	A	S	T	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																																		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	A	I	T	G	C	L	I	A	G	V	P	V	V	P	V	P	A	D	V	G	V	T	E	R	R	H	M	L	T	D	S	G	V	Q	A	W	L	G	P	L	P	D	D	P	A	G	L	P	H	I	P	V	R	T	H	A	R	S	W
Secondary structure
SS confidence
Disorder																																																								?			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	R	Y	P	E	P	S	P	G	A	I	A	M	V	V	Y	T	S	G	T	T	G	P	P	K	G	V	Q	L	S	R	R	A	I	A	A	D	L	D	A	L	A	E	A	W	Q	W	T	A	E	D	V	L	V	H	G	L	P	L	Y
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		H	V	H	G	L	V	L	G	L	L	G	S	L	R	F	G	N	R	F	V	H	T	G	K	P	T	P	A	G	Y	A	Q	A	C	Y	E	A	H	G	T	L	F	F	G	V	P	T	V	W	S	R	V	A	A	D	Q	A	A	A	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	L	K	P	A	R	L	L	V	S	G	S	A	A	L	P	V	P	V	F	D	K	L	V	Q	L	T	G	H	R	P	V	E	R	Y	G	A	S	E	S	L	I	T	L	S	T	R	A	D	G	E	R	R	P	G	W	V	G	L	P
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	A	G	V	Q	T	R	L	V	D	D	D	G	G	E	V	P	H	D	G	E	T	V	G	K	L	Q	V	R	G	P	T	L	F	D	G	Y	L	N	Q	P	D	A	T	A	A	A	F	D	A	D	S	W	Y	R	T	G	D	V	A
Secondary structure
SS confidence
Disorder																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		V	V	D	G	S	G	M	H	R	I	V	G	R	E	S	V	D	L	I	K	S	G	G	Y	R	V	G	A	G	E	I	E	T	V	L	L	G	H	P	D	V	A	E	A	A	V	V	G	V	P	D	D	D	L	G	Q	R	I	V	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.
Sequence		Y	V	V	G	S	A	N	V	D	A	D	G	L	I	N	F	V	A	Q	Q	L	S	V	H	K	R	P	R	E	V	R	I	V	D	A	L	P	R	N	A	L	G	K	V	L	K	K	Q	L	L	S	E	G
Secondary structure
SS confidence
Disorder																																																					?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 4%) Alpha helix ( 32%) Beta strand ( 23%) TM helix ( 9%) (View membrane topology prediction)

Rank Aligned region

PDB 6eqo chain B Region: 3 - 471 Aligned: 469 Modelled: 469 Confidence: 100.0% Identity: 22% PDB header:oxidoreductase Chain: B: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: tri-functional propionyl-coa synthase of erythrobacter sp. nap1 with2 bound nadp+ and phosphomethylphosphonic acid adenylate ester Phyre2 PDB 5es8 chain A Region: 4 - 471 Aligned: 449 Modelled: 461 Confidence: 100.0% Identity: 20% PDB header:ligase Chain: A: PDB Molecule:linear gramicidin synthetase subunit a; PDBTitle: crystal structure of the initiation module of lgra in the thiolation2 state Phyre2 PDB 6p1j chain A Region: 4 - 469 Aligned: 456 Modelled: 466 Confidence: 100.0% Identity: 23% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 5gxd chain A Region: 3 - 471 Aligned: 462 Modelled: 462 Confidence: 100.0% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:amp-dependent synthetase and ligase; PDBTitle: structure of acryloyl-coa lyase prpe from dinoroseobacter shibae dfl2 12 Phyre2 PDB 5ifi chain A Region: 4 - 471 Aligned: 467 Modelled: 468 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: crystal structure of acetyl-coa synthetase in complex with adenosine-2 5'-propylphosphate from cryptococcus neoformans h99 Phyre2 PDB 1pg4 chain A Region: 4 - 471 Aligned: 464 Modelled: 468 Confidence: 100.0% Identity: 20% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5ja2 chain A Region: 4 - 472 Aligned: 460 Modelled: 469 Confidence: 100.0% Identity: 23% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 1ry2 chain A Region: 4 - 472 Aligned: 465 Modelled: 469 Confidence: 100.0% Identity: 23% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5u89 chain A Region: 4 - 472 Aligned: 448 Modelled: 469 Confidence: 100.0% Identity: 24% PDB header:hydrolase/inhibitor Chain: A: PDB Molecule:amino acid adenylation domain protein; PDBTitle: crystal structure of a cross-module fragment from the dimodular nrps2 dhbf Phyre2 PDB 2vsq chain A Region: 4 - 472 Aligned: 457 Modelled: 469 Confidence: 100.0% Identity: 20% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 4wd1 chain A Region: 4 - 471 Aligned: 458 Modelled: 466 Confidence: 100.0% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:acetoacetate-coa ligase; PDBTitle: acetoacetyl-coa synthetase from streptomyces lividans Phyre2 PDB 3vnq chain A Region: 4 - 472 Aligned: 462 Modelled: 469 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: A: PDB Molecule:nrps adenylation protein cytc1; PDBTitle: co-crystal structure of nrps adenylation protein cytc1 with atp from2 streptomyces Phyre2 PDB 5wmm chain A Region: 4 - 471 Aligned: 451 Modelled: 466 Confidence: 100.0% Identity: 22% PDB header:biosynthetic protein Chain: A: PDB Molecule:nrps; PDBTitle: crystal structure of an adenylation domain interrupted by a2 methylation domain (ama4) from nonribosomal peptide synthetase tios Phyre2 PDB 3cw9 chain A domain 1 Region: 4 - 472 Aligned: 461 Modelled: 467 Confidence: 100.0% Identity: 28% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5x8g chain A Region: 4 - 472 Aligned: 455 Modelled: 467 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: A: PDB Molecule:2-succinylbenzoate--coa ligase; PDBTitle: binary complex structure of a double mutant i454ra456k of o-2 succinylbenzoate coa synthetase (mene) from bacillus subtilis bound3 with its product analogue osb-ncoa at 1.90 angstrom Phyre2 PDB 1mdb chain A Region: 4 - 471 Aligned: 460 Modelled: 468 Confidence: 100.0% Identity: 22% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 3e7w chain A Region: 4 - 472 Aligned: 460 Modelled: 469 Confidence: 100.0% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:d-alanine--poly(phosphoribitol) ligase subunit 1; PDBTitle: crystal structure of dlta: implications for the reaction mechanism of2 non-ribosomal peptide synthetase (nrps) adenylation domains Phyre2 PDB 4wv3 chain A Region: 4 - 471 Aligned: 457 Modelled: 468 Confidence: 100.0% Identity: 20% PDB header:ligase/ligase inhibitor Chain: A: PDB Molecule:anthranilate-coa ligase; PDBTitle: crystal structure of the anthranilate coa ligase auaeii in complex2 with anthranoyl-amp Phyre2 PDB 5wm7 chain A Region: 4 - 472 Aligned: 462 Modelled: 469 Confidence: 100.0% Identity: 27% PDB header:ligase Chain: A: PDB Molecule:salicylate-amp ligase; PDBTitle: crystal structure of cahj in complex with amp Phyre2 PDB 4zxi chain A Region: 4 - 472 Aligned: 449 Modelled: 469 Confidence: 100.0% Identity: 21% PDB header:biosynthetic protein Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: crystal structure of holo-ab3403 a four domain nonribosomal peptide2 synthetase bound to amp and glycine Phyre2 1 c6eqoB_ 2 c5es8A_ 3 c6p1jA_ 4 c5gxdA_ 5 c5ifiA_ 6 d1pg4a_ 7 c5ja2A_ 8 d1ry2a_ 9 c5u89A_ 10 c2vsqA_ 11 c4wd1A_ 12 c3vnqA_ 13 c5wmmA_ 14 d3cw9a1 15 c5x8gA_ 16 d1mdba_ 17 c3e7wA_ 18 c4wv3A_ 19 c5wm7A_ 20 c4zxiA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122