1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	S	K	P	F	A	P	R	R	L	Y	T	P	R	T	S	R	T	L	A	P	R	L	D	P	E	A	V	G	R	T	T	E	S	I	A	R	F	F	G	T	G	R	Y	L	L	V	Q	T	L	L	V	L	T	W	I	V	L	N	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	A	V	G	L	R	W	D	P	Y	P	F	I	L	L	N	L	A	F	S	T	Q	A	S	Y	A	A	P	L	I	L	L	A	Q	N	R	Q	E	K	R	D	R	A	V	F	E	E	D	R	R	R	A	A	Q	T	K	A	D	T	E
Secondary structure
SS confidence
Disorder			?																																	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	N	A	R	E	L	A	A	L	R	L	A	I	G	E	V	P	T	R	D	Y	L	R	H	E	L	D	S	L	R	A	L	L	A	E	L	Q	P	T	D	P	D	V	A	Q	P	R	V	A	D	E	A	E	Q	H	A	K	K	S	G
Secondary structure
SS confidence
Disorder																																					?	?	?	?	?	?						?	?	?	?					?	?	?	?	?	?
Disorder confidence
Sequence
Secondary structure
SS confidence
Disorder
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 25%) Alpha helix ( 81%) Beta strand ( 0%) TM helix ( 24%) (View membrane topology prediction)

Rank Aligned region

PDB 6cfw chain A Region: 44 - 88 Aligned: 44 Modelled: 45 Confidence: 51.5% Identity: 18% PDB header:membrane protein Chain: A: PDB Molecule:monovalent cation/h+ antiporter subunit e; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 4cbf chain E Region: 38 - 59 Aligned: 22 Modelled: 22 Confidence: 44.5% Identity: 23% PDB header:virus Chain: E: PDB Molecule:envelope protein e; PDBTitle: near-atomic resolution cryo-em structure of dengue serotype 4 virus Phyre2 PDB 5ire chain A Region: 38 - 59 Aligned: 22 Modelled: 22 Confidence: 34.6% Identity: 27% PDB header:virus Chain: A: PDB Molecule:e protein; PDBTitle: the cryo-em structure of zika virus Phyre2 PDB 5o6v chain C Region: 38 - 59 Aligned: 22 Modelled: 22 Confidence: 32.3% Identity: 32% PDB header:virus Chain: C: PDB Molecule:envelope protein; PDBTitle: the cryo-em structure of tick-borne encephalitis virus complexed with2 fab fragment of neutralizing antibody 19/1786 Phyre2 PDB 6bwi chain A Region: 69 - 140 Aligned: 72 Modelled: 72 Confidence: 28.0% Identity: 8% PDB header:membrane protein Chain: A: PDB Molecule:transient receptor potential cation channel subfamily m PDBTitle: 3.7 angstrom cryoem structure of full length human trpm4 Phyre2 PDB 5wsn chain C Region: 38 - 59 Aligned: 22 Modelled: 22 Confidence: 27.8% Identity: 23% PDB header:virus Chain: C: PDB Molecule:e protein; PDBTitle: structure of japanese encephalitis virus Phyre2 PDB 1k4t chain A domain 1 Region: 94 - 167 Aligned: 68 Modelled: 74 Confidence: 26.0% Identity: 18% Fold: Long alpha-hairpin Superfamily: Eukaryotic DNA topoisomerase I, dispensable insert domain Family: Eukaryotic DNA topoisomerase I, dispensable insert domain Phyre2 PDB 4b03 chain A Region: 38 - 59 Aligned: 22 Modelled: 22 Confidence: 20.4% Identity: 32% PDB header:virus Chain: A: PDB Molecule:dengue virus 1 e protein; PDBTitle: 6a electron cryomicroscopy structure of immature dengue virus serotype2 1 Phyre2 PDB 6co7 chain C Region: 43 - 139 Aligned: 97 Modelled: 97 Confidence: 18.2% Identity: 9% PDB header:membrane protein Chain: C: PDB Molecule:predicted protein; PDBTitle: structure of the nvtrpm2 channel in complex with ca2+ Phyre2 PDB 4ltp chain C Region: 72 - 126 Aligned: 55 Modelled: 55 Confidence: 17.9% Identity: 16% PDB header:transport protein Chain: C: PDB Molecule:ion transport protein; PDBTitle: bacterial sodium channel in high calcium, i222 space group, crystal 2 Phyre2 PDB 1p58 chain C Region: 38 - 83 Aligned: 46 Modelled: 46 Confidence: 17.1% Identity: 24% PDB header:virus Chain: C: PDB Molecule:major envelope protein e; PDBTitle: complex organization of dengue virus membrane proteins as revealed by2 9.5 angstrom cryo-em reconstruction Phyre2 PDB 6rdu chain 9 Region: 37 - 72 Aligned: 35 Modelled: 36 Confidence: 17.1% Identity: 17% PDB header:proton transport Chain: 9: PDB Molecule:asa-9: polytomella f-atp synthase associated subunit 9; PDBTitle: cryo-em structure of polytomella f-atp synthase, rotary substate 1e,2 monomer-masked refinement Phyre2 PDB 2mxb chain A Region: 60 - 96 Aligned: 36 Modelled: 37 Confidence: 16.9% Identity: 22% PDB header:membrane protein Chain: A: PDB Molecule:erythropoietin receptor; PDBTitle: structure of the transmembrane domain of the mouse erythropoietin2 receptor Phyre2 PDB 2w2e chain A Region: 11 - 94 Aligned: 83 Modelled: 84 Confidence: 15.7% Identity: 17% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin pip2-7 7; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin, aqy1, in a2 closed conformation at ph 3.5 Phyre2 PDB 4ev6 chain E Region: 33 - 87 Aligned: 55 Modelled: 55 Confidence: 15.6% Identity: 11% PDB header:metal transport Chain: E: PDB Molecule:magnesium transport protein cora; PDBTitle: the complete structure of cora magnesium transporter from2 methanocaldococcus jannaschii Phyre2 PDB 3m9b chain K Region: 118 - 159 Aligned: 42 Modelled: 42 Confidence: 15.4% Identity: 19% PDB header:chaperone Chain: K: PDB Molecule:proteasome-associated atpase; PDBTitle: crystal structure of the amino terminal coiled coil domain and the2 inter domain of the mycobacterium tuberculosis proteasomal atpase mpa Phyre2 PDB 1dfm chain A Region: 66 - 80 Aligned: 15 Modelled: 15 Confidence: 15.2% Identity: 40% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: Restriction endonuclease BglII Phyre2 PDB 2m6x chain C Region: 36 - 79 Aligned: 44 Modelled: 44 Confidence: 14.0% Identity: 20% PDB header:membrane protein Chain: C: PDB Molecule:p7; PDBTitle: structure of the p7 channel of hepatitis c virus, genotype 5a Phyre2 PDB 2kn8 chain A Region: 70 - 94 Aligned: 25 Modelled: 25 Confidence: 11.4% Identity: 20% PDB header:protein binding, dna binding protein Chain: A: PDB Molecule:dna cleavage and packaging protein large subunit, ul89; PDBTitle: nmr structure of the c-terminal domain of pul89 Phyre2 PDB 6bwi chain B Region: 43 - 140 Aligned: 98 Modelled: 98 Confidence: 10.5% Identity: 16% PDB header:membrane protein Chain: B: PDB Molecule:transient receptor potential cation channel subfamily m PDBTitle: 3.7 angstrom cryoem structure of full length human trpm4 Phyre2 1 c6cfwA_ 2 c4cbfE_ 3 c5ireA_ 4 c5o6vC_ 5 c6bwiA_ 6 c5wsnC_ 7 d1k4ta1 8 c4b03A_ 9 c6co7C_ 10 c4ltpC_ 11 c1p58C_ 12 c6rdu9_ 13 c2mxbA_ 14 c2w2eA_ 15 c4ev6E_ 16 c3m9bK_ 17 d1dfma_ 18 c2m6xC_ 19 c2kn8A_ 20 c6bwiB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66