1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	G	E	V	S	A	I	V	L	A	A	S	Q	A	A	E	E	G	G	E	S	S	N	F	L	I	P	N	G	T	F	F	V	V	L	A	I	F	L	V	V	L	A	V	I	G	T	F	V	V	P	P	I	L	K	V	L	R	E	R
Secondary structure
SS confidence
Disorder		?	?	?							?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	A	M	V	A	K	T	L	A	D	N	K	K	S	D	E	Q	F	A	A	A	Q	A	D	Y	D	E	A	M	T	E	A	R	V	Q	A	S	S	L	R	D	N	A	R	A	D	G	R	K	V	I	E	D	A	R	V	R	A	E	Q
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.
Sequence		Q	V	A	S	T	L	Q	T	A	H	E	Q	L	K	R	E	R	D	A	V	E	L	D	L	R	A	H	V	G	T	M	S	A	T	L	A	S	R	I	L	G	V	D	L	T	A	S	A	A	T	R
Secondary structure
SS confidence
Disorder																																											?	?	?	?				?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 14%) Alpha helix ( 91%) Beta strand ( 0%) TM helix ( 13%) (View membrane topology prediction)

Rank Aligned region

PDB 6fki chain P Region: 20 - 160 Aligned: 141 Modelled: 141 Confidence: 100.0% Identity: 17% PDB header:membrane protein Chain: P: PDB Molecule:atp synthase subunit c, chloroplastic; PDBTitle: chloroplast f1fo conformation 3 Phyre2 PDB 5t4o chain J Region: 26 - 167 Aligned: 142 Modelled: 142 Confidence: 100.0% Identity: 19% PDB header:hydrolase Chain: J: PDB Molecule:atp synthase subunit b; PDBTitle: autoinhibited e. coli atp synthase state 1 Phyre2 PDB 6fki chain B Region: 26 - 171 Aligned: 146 Modelled: 146 Confidence: 100.0% Identity: 14% PDB header:membrane protein Chain: B: PDB Molecule:atp synthase subunit beta, chloroplastic; PDBTitle: chloroplast f1fo conformation 3 Phyre2 PDB 6b8h chain B Region: 8 - 171 Aligned: 159 Modelled: 161 Confidence: 99.8% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:atp synthase subunit alpha, mitochondrial; PDBTitle: mosaic model of yeast mitochondrial atp synthase monomer Phyre2 PDB 6j5i chain B Region: 32 - 168 Aligned: 137 Modelled: 137 Confidence: 99.4% Identity: 9% PDB header:membrane protein Chain: B: PDB Molecule:atp synthase subunit alpha, mitochondrial; PDBTitle: cryo-em structure of the mammalian dp-state atp synthase Phyre2 PDB 1l2p chain A Region: 87 - 145 Aligned: 59 Modelled: 59 Confidence: 98.1% Identity: 20% Fold: Single transmembrane helix Superfamily: F1F0 ATP synthase subunit B, membrane domain Family: F1F0 ATP synthase subunit B, membrane domain Phyre2 PDB 5arh chain T Region: 32 - 150 Aligned: 119 Modelled: 119 Confidence: 98.0% Identity: 12% PDB header:hydrolase Chain: T: PDB Molecule:atp synthase f(0) complex subunit b1, mitochondrial; PDBTitle: bovine mitochondrial atp synthase state 2a Phyre2 PDB 1b9u chain A Region: 28 - 57 Aligned: 30 Modelled: 30 Confidence: 97.8% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:protein (atp synthase); PDBTitle: membrane domain of the subunit b of the e.coli atp synthase Phyre2 PDB 5lqy chain V Region: 33 - 160 Aligned: 128 Modelled: 128 Confidence: 97.5% Identity: 15% PDB header:hydrolase Chain: V: PDB Molecule:atp synthase subunit b; PDBTitle: structure of f-atpase from pichia angusta, in state2 Phyre2 PDB 4dt0 chain A Region: 84 - 162 Aligned: 79 Modelled: 79 Confidence: 97.2% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:v-type atp synthase subunit e; PDBTitle: the structure of the peripheral stalk subunit e from pyrococcus2 horikoshii Phyre2 PDB 3k5b chain E Region: 79 - 167 Aligned: 89 Modelled: 89 Confidence: 96.5% Identity: 13% PDB header:hydrolase Chain: E: PDB Molecule:v-type atp synthase subunit e; PDBTitle: crystal structure of the peripheral stalk of thermus thermophilus h+-2 atpase/synthase Phyre2 PDB 4dl0 chain J Region: 76 - 162 Aligned: 87 Modelled: 87 Confidence: 95.6% Identity: 6% PDB header:hydrolase Chain: J: PDB Molecule:v-type proton atpase subunit e; PDBTitle: crystal structure of the heterotrimeric egchead peripheral stalk2 complex of the yeast vacuolar atpase Phyre2 PDB 2khk chain A Region: 57 - 103 Aligned: 47 Modelled: 47 Confidence: 93.7% Identity: 19% PDB header:transport protein Chain: A: PDB Molecule:atp synthase subunit b; PDBTitle: nmr solution structure of the b30-82 domain of subunit b of2 escherichia coli f1fo atp synthase Phyre2 PDB 6b2z chain B Region: 33 - 78 Aligned: 46 Modelled: 46 Confidence: 93.6% Identity: 15% PDB header:membrane protein Chain: B: PDB Molecule:atp synthase subunit c, mitochondrial; PDBTitle: cryo-em structure of the dimeric fo region of yeast mitochondrial atp2 synthase Phyre2 PDB 4dl0 chain G Region: 76 - 167 Aligned: 92 Modelled: 92 Confidence: 92.7% Identity: 12% PDB header:hydrolase Chain: G: PDB Molecule:v-type proton atpase subunit g; PDBTitle: crystal structure of the heterotrimeric egchead peripheral stalk2 complex of the yeast vacuolar atpase Phyre2 PDB 2oar chain A Region: 19 - 84 Aligned: 64 Modelled: 66 Confidence: 92.4% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:large-conductance mechanosensitive channel; PDBTitle: mechanosensitive channel of large conductance (mscl) Phyre2 PDB 1y4c chain A Region: 24 - 147 Aligned: 124 Modelled: 124 Confidence: 92.1% Identity: 11% PDB header:de novo protein Chain: A: PDB Molecule:maltose binding protein fused with designed PDBTitle: designed helical protein fusion mbp Phyre2 PDB 2oar chain A domain 1 Region: 19 - 59 Aligned: 41 Modelled: 41 Confidence: 90.5% Identity: 15% Fold: Gated mechanosensitive channel Superfamily: Gated mechanosensitive channel Family: Gated mechanosensitive channel Phyre2 PDB 3k5b chain B Region: 64 - 160 Aligned: 97 Modelled: 97 Confidence: 88.6% Identity: 21% PDB header:hydrolase Chain: B: PDB Molecule:v-type atp synthase, subunit (vapc-therm); PDBTitle: crystal structure of the peripheral stalk of thermus thermophilus h+-2 atpase/synthase Phyre2 PDB 4cg4 chain D Region: 55 - 157 Aligned: 102 Modelled: 103 Confidence: 87.4% Identity: 16% PDB header:actin-binding protein Chain: D: PDB Molecule:pyrin; PDBTitle: crystal structure of the chs-b30.2 domains of trim20 Phyre2 1 c6fkip_ 2 c5t4oJ_ 3 c6fkib_ 4 c6b8hb_ 5 c6j5ib_ 6 d1l2pa_ 7 c5arhT_ 8 c1b9uA_ 9 c5lqyV_ 10 c4dt0A_ 11 c3k5bE_ 12 c4dl0J_ 13 c2khkA_ 14 c6b2zb_ 15 c4dl0G_ 16 c2oarA_ 17 c1y4cA_ 18 d2oara1 19 c3k5bB_ 20 c4cg4D_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99