1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	T	F	I	G	Q	L	F	G	F	A	V	I	V	Y	L	V	W	R	F	I	V	P	L	V	G	R	L	M	S	A	R	Q	D	T	V	R	Q	Q	L	A	D	A	A	A	A	A	D	R	L	A	E	A	S	Q	A	H	T	K
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	L	E	D	A	K	S	E	A	H	R	V	V	E	E	A	R	T	D	A	E	R	I	A	E	Q	L	E	A	Q	A	D	V	E	A	E	R	I	K	M	Q	G	A	R	Q	V	D	L	I	R	A	Q	L	T	R	Q	L	R	L	E
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	G	H	E	S	V	R	Q	A	R	E	L	V	R	N	H	V	A	D	Q	A	Q	Q	S	A	T	V	D	R	F	L	D	Q	L	D	A	M	A	P	A	T	A	D	V	D	Y	P	L	L	A	K	M	R	S	A	S	R	R	A	L
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?															?	?	?	?	?	?	?	?	?	?	?						?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		T	S	L	V	D	W	F	G	T	M	A	Q	D	L	D	H	Q	G	L	T	T	L	A	G	E	L	V	S	V	A	R	L	L	D	R	E	A	V	V	T	R	Y	L	T	V	P	A	E	D	A	T	P	R	I	R	L	I	E	R	L
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?	?	?																?	?				?							?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		V	S	G	K	V	G	A	P	T	L	E	V	L	R	T	A	V	S	K	R	W	S	A	N	S	D	L	I	D	A	I	E	H	V	S	R	Q	A	L	L	E	L	A	E	R	A	G	Q	V	D	E	V	E	D	Q	L	F	R	F	S
Secondary structure
SS confidence
Disorder			?	?		?												?	?	?	?	?	?	?	?																							?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	I	L	D	V	Q	P	R	L	A	I	L	L	G	D	C	A	V	P	A	E	G	R	V	R	L	L	R	K	V	L	E	R	A	D	S	T	V	N	P	V	V	V	A	L	L	S	H	T	V	E	L	L	R	G	Q	A	V	E	E
Secondary structure
SS confidence
Disorder																	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		A	V	L	F	L	A	E	V	A	V	A	R	R	G	E	I	V	A	Q	V	G	A	A	A	E	L	S	D	A	Q	R	T	R	L	T	E	V	L	S	R	I	Y	G	H	P	V	T	V	Q	L	H	I	D	A	A	L	L	G	G	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.
Sequence		S	I	A	V	G	D	E	V	I	D	G	T	L	S	S	R	L	A	A	A	E	A	R	L	P	D
Secondary structure
SS confidence
Disorder																									?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 13%) Alpha helix ( 77%) Beta strand ( 5%) TM helix ( 5%) (View membrane topology prediction)

Rank Aligned region

PDB 6fkf chain D Region: 269 - 444 Aligned: 173 Modelled: 176 Confidence: 100.0% Identity: 18% PDB header:membrane protein Chain: D: PDB Molecule:atp synthase subunit beta, chloroplastic; PDBTitle: chloroplast f1fo conformation 1 Phyre2 PDB 6rdl chain P Region: 270 - 446 Aligned: 175 Modelled: 175 Confidence: 100.0% Identity: 23% PDB header:proton transport Chain: P: PDB Molecule:mitochondrial atp synthase subunit oscp; PDBTitle: cryo-em structure of polytomella f-atp synthase, rotary substate 1b,2 monomer-masked refinement Phyre2 PDB 5t4o chain L Region: 270 - 431 Aligned: 157 Modelled: 162 Confidence: 100.0% Identity: 19% PDB header:hydrolase Chain: L: PDB Molecule:atp synthase subunit delta; PDBTitle: autoinhibited e. coli atp synthase state 1 Phyre2 PDB 6fki chain B Region: 1 - 159 Aligned: 158 Modelled: 159 Confidence: 100.0% Identity: 11% PDB header:membrane protein Chain: B: PDB Molecule:atp synthase subunit beta, chloroplastic; PDBTitle: chloroplast f1fo conformation 3 Phyre2 PDB 5t4o chain J Region: 2 - 154 Aligned: 152 Modelled: 153 Confidence: 99.9% Identity: 26% PDB header:hydrolase Chain: J: PDB Molecule:atp synthase subunit b; PDBTitle: autoinhibited e. coli atp synthase state 1 Phyre2 PDB 6fki chain P Region: 2 - 134 Aligned: 133 Modelled: 133 Confidence: 99.9% Identity: 11% PDB header:membrane protein Chain: P: PDB Molecule:atp synthase subunit c, chloroplastic; PDBTitle: chloroplast f1fo conformation 3 Phyre2 PDB 6b8h chain O Region: 270 - 416 Aligned: 146 Modelled: 147 Confidence: 99.9% Identity: 21% PDB header:membrane protein Chain: O: PDB Molecule:atp synthase subunit 5, mitochondrial; PDBTitle: mosaic model of yeast mitochondrial atp synthase monomer Phyre2 PDB 2wss chain S Region: 269 - 444 Aligned: 153 Modelled: 155 Confidence: 99.9% Identity: 22% PDB header:hydrolase Chain: S: PDB Molecule:atp synthase subunit o, mitochondrial; PDBTitle: the structure of the membrane extrinsic region of bovine atp synthase Phyre2 PDB 5lqz chain U Region: 270 - 413 Aligned: 142 Modelled: 144 Confidence: 99.9% Identity: 17% PDB header:hydrolase Chain: U: PDB Molecule:atp synthase oscp subunit; PDBTitle: structure of f-atpase from pichia angusta, state1 Phyre2 PDB 6b8h chain B Region: 5 - 158 Aligned: 149 Modelled: 151 Confidence: 99.9% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:atp synthase subunit alpha, mitochondrial; PDBTitle: mosaic model of yeast mitochondrial atp synthase monomer Phyre2 PDB 5dn6 chain H Region: 267 - 375 Aligned: 107 Modelled: 109 Confidence: 99.8% Identity: 17% PDB header:hydrolase Chain: H: PDB Molecule:atp synthase subunit delta; PDBTitle: atp synthase from paracoccus denitrificans Phyre2 PDB 2jmx chain A Region: 270 - 380 Aligned: 108 Modelled: 111 Confidence: 99.7% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:atp synthase o subunit, mitochondrial; PDBTitle: oscp-nt (1-120) in complex with n-terminal (1-25) alpha2 subunit from f1-atpase Phyre2 PDB 6rdv chain P Region: 270 - 374 Aligned: 103 Modelled: 105 Confidence: 99.7% Identity: 22% PDB header:proton transport Chain: P: PDB Molecule:mitochondrial atp synthase subunit oscp; PDBTitle: cryo-em structure of polytomella f-atp synthase, rotary substate 1e,2 focussed refinement of f1 head and rotor Phyre2 PDB 1abv chain A Region: 269 - 375 Aligned: 102 Modelled: 107 Confidence: 99.7% Identity: 13% Fold: ATPD N-terminal domain-like Superfamily: N-terminal domain of the delta subunit of the F1F0-ATP synthase Family: N-terminal domain of the delta subunit of the F1F0-ATP synthase Phyre2 PDB 2a7u chain B Region: 269 - 375 Aligned: 102 Modelled: 107 Confidence: 99.7% Identity: 13% PDB header:hydrolase Chain: B: PDB Molecule:atp synthase delta chain; PDBTitle: nmr solution structure of the e.coli f-atpase delta subunit n-terminal2 domain in complex with alpha subunit n-terminal 22 residues Phyre2 PDB 6j5i chain B Region: 8 - 163 Aligned: 151 Modelled: 156 Confidence: 99.5% Identity: 9% PDB header:membrane protein Chain: B: PDB Molecule:atp synthase subunit alpha, mitochondrial; PDBTitle: cryo-em structure of the mammalian dp-state atp synthase Phyre2 PDB 5lqy chain V Region: 8 - 158 Aligned: 146 Modelled: 151 Confidence: 98.5% Identity: 18% PDB header:hydrolase Chain: V: PDB Molecule:atp synthase subunit b; PDBTitle: structure of f-atpase from pichia angusta, in state2 Phyre2 PDB 5arh chain T Region: 5 - 159 Aligned: 146 Modelled: 155 Confidence: 98.5% Identity: 11% PDB header:hydrolase Chain: T: PDB Molecule:atp synthase f(0) complex subunit b1, mitochondrial; PDBTitle: bovine mitochondrial atp synthase state 2a Phyre2 PDB 1l2p chain A Region: 60 - 118 Aligned: 59 Modelled: 59 Confidence: 98.4% Identity: 27% Fold: Single transmembrane helix Superfamily: F1F0 ATP synthase subunit B, membrane domain Family: F1F0 ATP synthase subunit B, membrane domain Phyre2 PDB 4dt0 chain A Region: 58 - 153 Aligned: 94 Modelled: 96 Confidence: 97.6% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:v-type atp synthase subunit e; PDBTitle: the structure of the peripheral stalk subunit e from pyrococcus2 horikoshii Phyre2 1 c6fkfd_ 2 c6rdlP_ 3 c5t4oL_ 4 c6fkib_ 5 c5t4oJ_ 6 c6fkip_ 7 c6b8ho_ 8 c2wssS_ 9 c5lqzU_ 10 c6b8hb_ 11 c5dn6H_ 12 c2jmxA_ 13 c6rdvP_ 14 d1abva_ 15 c2a7uB_ 16 c6j5ib_ 17 c5lqyV_ 18 c5arhT_ 19 d1l2pa_ 20 c4dt0A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99