1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	T	A	P	A	I	C	N	T	T	E	T	V	H	G	I	A	T	S	L	G	A	V	A	R	Q	A	S	L	P	R	I	V	G	T	V	V	G	I	T	V	L	V	V	V	A	L	L	V	P	V	P	T	A	V	E	L	R	D	W
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	K	S	L	G	A	W	F	P	L	A	F	L	L	V	H	T	V	V	T	V	P	P	F	P	R	T	A	F	T	L	A	A	G	L	L	F	G	S	V	V	G	V	F	I	A	V	V	G	S	T	A	S	A	V	I	A	M	L	L
Secondary structure
SS confidence
Disorder																								?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	R	A	T	G	W	Q	L	N	S	L	V	R	R	R	A	I	N	R	L	D	E	R	L	R	E	R	G	W	L	A	I	L	S	L	R	L	I	P	V	V	P	F	A	A	I	N	Y	A	A	G	A	S	G	V	R	I	L	S	F
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	W	A	T	L	A	G	L	L	P	G	T	A	A	V	V	I	L	G	D	A	F	A	G	S	G	S	P	L	L	I	L	V	S	V	C	T	G	A	L	G	L	T	G	L	V	Y	E	I	R	N	Y	R	R	Q	H	R	R	M	P
Secondary structure
SS confidence
Disorder																																																									?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.
Sequence		G	Y	D	D	P	V	R	E	P	A	L	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 15%) Alpha helix ( 76%) Beta strand ( 0%) TM helix ( 54%) (View membrane topology prediction)

Rank Aligned region

PDB 5d7t chain C Region: 85 - 116 Aligned: 32 Modelled: 32 Confidence: 90.1% Identity: 25% PDB header:transport protein Chain: C: PDB Molecule:s-component for folate; PDBTitle: folate ecf transporter: apo state Phyre2 PDB 4huq chain S Region: 85 - 117 Aligned: 33 Modelled: 33 Confidence: 86.6% Identity: 15% PDB header:hydrolase Chain: S: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a transporter Phyre2 PDB 4z7f chain D Region: 85 - 198 Aligned: 110 Modelled: 114 Confidence: 84.5% Identity: 10% PDB header:transport protein Chain: D: PDB Molecule:folate ecf transporter; PDBTitle: crystal structure of folt bound with folic acid Phyre2 PDB 3p5n chain A Region: 85 - 116 Aligned: 32 Modelled: 32 Confidence: 64.1% Identity: 25% PDB header:transport protein Chain: A: PDB Molecule:riboflavin uptake protein; PDBTitle: structure and mechanism of the s component of a bacterial ecf2 transporter Phyre2 PDB 4hzu chain S Region: 85 - 202 Aligned: 112 Modelled: 118 Confidence: 61.1% Identity: 11% PDB header:hydrolase, transport protein Chain: S: PDB Molecule:predicted membrane protein; PDBTitle: structure of a bacterial energy-coupling factor transporter Phyre2 PDB 1xme chain A domain 1 Region: 128 - 249 Aligned: 102 Modelled: 106 Confidence: 59.4% Identity: 17% Fold: Cytochrome c oxidase subunit I-like Superfamily: Cytochrome c oxidase subunit I-like Family: Cytochrome c oxidase subunit I-like Phyre2 PDB 2jp3 chain A Region: 209 - 252 Aligned: 44 Modelled: 44 Confidence: 35.3% Identity: 18% PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles Phyre2 PDB 6c14 chain A Region: 210 - 238 Aligned: 29 Modelled: 29 Confidence: 30.8% Identity: 14% PDB header:membrane protein, metal transport Chain: A: PDB Molecule:protocadherin-15; PDBTitle: cryoem structure of mouse pcdh15-1ec-lhfpl5 complex Phyre2 PDB 6hum chain B Region: 29 - 174 Aligned: 146 Modelled: 146 Confidence: 17.7% Identity: 12% PDB header:proton transport Chain: B: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 2; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 2b2h chain A Region: 71 - 245 Aligned: 174 Modelled: 175 Confidence: 16.6% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter; PDBTitle: ammonium transporter amt-1 from a. fulgidus (as) Phyre2 PDB 5n9y chain B Region: 134 - 233 Aligned: 99 Modelled: 100 Confidence: 11.9% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:zinc transport protein zntb; PDBTitle: the full-length structure of zntb Phyre2 PDB 3rko chain M Region: 29 - 169 Aligned: 141 Modelled: 141 Confidence: 11.7% Identity: 11% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit m; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 1cii chain A Region: 135 - 204 Aligned: 65 Modelled: 70 Confidence: 8.9% Identity: 14% PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia Phyre2 PDB 5edl chain A Region: 87 - 244 Aligned: 154 Modelled: 158 Confidence: 8.7% Identity: 18% PDB header:transport protein Chain: A: PDB Molecule:putative hmp/thiamine permease protein ykoe; PDBTitle: crystal structure of an s-component of ecf transporter Phyre2 PDB 4j8c chain A Region: 49 - 65 Aligned: 17 Modelled: 17 Confidence: 8.4% Identity: 29% PDB header:chaperone Chain: A: PDB Molecule:hsc70-interacting protein; PDBTitle: crystal structure of the dimerization domain of hsc70-interacting2 protein Phyre2 PDB 4j8c chain B Region: 49 - 65 Aligned: 17 Modelled: 17 Confidence: 8.4% Identity: 29% PDB header:chaperone Chain: B: PDB Molecule:hsc70-interacting protein; PDBTitle: crystal structure of the dimerization domain of hsc70-interacting2 protein Phyre2 PDB 4dji chain A Region: 174 - 252 Aligned: 79 Modelled: 79 Confidence: 8.1% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:probable glutamate/gamma-aminobutyrate antiporter; PDBTitle: structure of glutamate-gaba antiporter gadc Phyre2 PDB 1cii chain A domain 1 Region: 53 - 120 Aligned: 62 Modelled: 68 Confidence: 6.8% Identity: 19% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 5oon chain A Region: 67 - 244 Aligned: 144 Modelled: 149 Confidence: 6.5% Identity: 18% PDB header:membrane protein Chain: A: PDB Molecule:undecaprenyl-diphosphatase; PDBTitle: structure of undecaprenyl-pyrophosphate phosphatase, baca Phyre2 PDB 2na6 chain B Region: 213 - 237 Aligned: 25 Modelled: 25 Confidence: 6.2% Identity: 28% PDB header:apoptosis Chain: B: PDB Molecule:tumor necrosis factor receptor superfamily member 6; PDBTitle: transmembrane domain of mouse fas/cd95 death receptor Phyre2 1 c5d7tC_ 2 c4huqS_ 3 c4z7fD_ 4 c3p5nA_ 5 c4hzuS_ 6 d1xmea1 7 c2jp3A_ 8 c6c14A_ 9 c6humB_ 10 c2b2hA_ 11 c5n9yB_ 12 c3rkoM_ 13 c1ciiA_ 14 c5edlA_ 15 c4j8cA_ 16 c4j8cB_ 17 c4djiA_ 18 d1ciia1 19 c5oonA_ 20 c2na6B_ 21 22 23 24 25 26