1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	S	I	D	V	P	E	R	A	D	L	E	Q	V	R	G	R	W	R	N	A	V	A	G	V	L	S	K	S	N	R	T	D	S	A	Q	L	G	D	H	P	E	R	L	L	D	T	Q	T	A	D	G	F	A	I	R	A	L	Y	T
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?																	?		?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	F	D	E	L	P	E	P	P	L	P	G	Q	W	P	F	V	R	G	G	D	P	L	R	D	V	H	S	G	W	K	V	A	E	A	F	P	A	N	G	A	T	A	D	T	N	A	A	V	L	A	A	L	G	E	G	V	S	A	L
Secondary structure
SS confidence
Disorder					?	?	?	?	?													?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	I	R	V	G	E	S	G	V	A	P	D	R	L	T	A	L	L	S	G	V	Y	L	N	L	A	P	V	I	L	D	A	G	A	D	Y	R	P	A	C	D	V	M	L	A	L	V	A	Q	L	D	P	G	Q	R	D	T	L	S	I
Secondary structure
SS confidence
Disorder																																																					?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		D	L	G	A	D	P	L	T	A	S	L	R	D	R	P	A	P	P	I	E	E	V	V	A	V	A	S	R	A	A	G	E	R	G	L	R	A	I	T	V	D	G	P	A	F	H	N	L	G	A	T	A	A	T	E	L	A	A	T	V
Secondary structure
SS confidence
Disorder																																																	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	A	A	V	A	Y	L	R	V	L	T	E	S	G	L	V	V	S	D	A	L	R	Q	I	S	F	R	L	A	A	D	D	D	Q	F	M	T	L	A	K	M	R	A	L	R	Q	L	W	A	R	V	A	E	V	V	G	D	P	G	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		G	A	A	V	V	H	A	E	T	S	L	P	M	M	T	Q	R	D	P	W	V	N	M	L	R	C	T	L	A	A	F	G	A	G	V	G	G	A	D	T	V	L	V	H	P	F	D	V	A	I	P	G	G	F	P	G	T	A	A	G
Secondary structure
SS confidence
Disorder																																																					?	?		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		F	A	R	R	I	A	R	N	T	Q	L	L	L	L	E	E	S	H	V	G	R	V	L	D	P	A	G	G	S	W	F	V	E	E	L	T	D	R	L	A	R	R	A	W	Q	R	F	Q	A	I	E	A	R	G	G	F	V	E	A	H
Secondary structure
SS confidence
Disorder																																																							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		D	F	L	A	G	Q	I	A	E	C	A	A	R	R	A	D	D	I	A	H	R	R	L	A	I	T	G	V	N	E	Y	P	N	L	G	E	P	A	L	P	P	G	D	P	T	S	P	V	R	R	Y	A	A	G	F	E	A	L	R	D
Secondary structure
SS confidence
Disorder																					?																?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		R	S	D	H	H	L	A	R	T	G	A	R	P	R	V	L	L	L	P	L	G	P	L	A	E	H	N	I	R	T	T	F	A	T	N	L	L	A	S	G	G	I	E	A	I	D	P	G	T	V	D	A	G	T	V	G	N	A	V	A
Secondary structure
SS confidence
Disorder									?	?													?																										?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		D	A	G	S	P	S	V	A	V	I	C	G	T	D	A	R	Y	R	D	E	V	A	D	I	V	Q	A	A	R	A	A	G	V	S	R	V	Y	L	A	G	P	E	K	A	L	G	D	A	A	H	R	P	D	E	F	L	T	A	K	I
Secondary structure
SS confidence
Disorder																																															?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.
Sequence		N	V	V	Q	A	L	S	N	L	L	T	R	L	G	A
Secondary structure
SS confidence
Disorder															?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 11%) Alpha helix ( 48%) Beta strand ( 11%)

Rank Aligned region

PDB 6req chain B Region: 2 - 614 Aligned: 600 Modelled: 613 Confidence: 100.0% Identity: 33% PDB header:isomerase Chain: B: PDB Molecule:protein (methylmalonyl-coa mutase); PDBTitle: methylmalonyl-coa mutase, 3-carboxypropyl-coa inhibitor complex Phyre2 PDB 3bic chain A Region: 10 - 613 Aligned: 585 Modelled: 594 Confidence: 100.0% Identity: 26% PDB header:isomerase Chain: A: PDB Molecule:methylmalonyl-coa mutase, mitochondrial precursor; PDBTitle: crystal structure of human methylmalonyl-coa mutase Phyre2 PDB 1e1c chain A Region: 2 - 613 Aligned: 588 Modelled: 591 Confidence: 100.0% Identity: 26% PDB header:isomerase Chain: A: PDB Molecule:methylmalonyl-coa mutase alpha chain; PDBTitle: methylmalonyl-coa mutase h244a mutant Phyre2 PDB 7req chain B domain 1 Region: 2 - 460 Aligned: 451 Modelled: 459 Confidence: 100.0% Identity: 34% Fold: TIM beta/alpha-barrel Superfamily: Cobalamin (vitamin B12)-dependent enzymes Family: Methylmalonyl-CoA mutase, N-terminal (CoA-binding) domain Phyre2 PDB 7req chain A domain 1 Region: 2 - 542 Aligned: 509 Modelled: 520 Confidence: 100.0% Identity: 27% Fold: TIM beta/alpha-barrel Superfamily: Cobalamin (vitamin B12)-dependent enzymes Family: Methylmalonyl-CoA mutase, N-terminal (CoA-binding) domain Phyre2 PDB 4r3u chain B Region: 8 - 505 Aligned: 481 Modelled: 493 Confidence: 100.0% Identity: 26% PDB header:isomerase Chain: B: PDB Molecule:2-hydroxyisobutyryl-coa mutase large subunit; PDBTitle: crystal structure of 2-hydroxyisobutyryl-coa mutase Phyre2 PDB 4xc8 chain B Region: 45 - 521 Aligned: 464 Modelled: 477 Confidence: 100.0% Identity: 22% PDB header:isomerase Chain: B: PDB Molecule:isobutyryl-coa mutase fused; PDBTitle: isobutyryl-coa mutase fused with bound butyryl-coa, gdp, and mg and2 without cobalamin (apo-icmf/gdp) Phyre2 PDB 7req chain B domain 2 Region: 458 - 614 Aligned: 152 Modelled: 157 Confidence: 100.0% Identity: 29% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 7req chain A domain 2 Region: 469 - 613 Aligned: 144 Modelled: 145 Confidence: 100.0% Identity: 15% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 1ccw chain A Region: 491 - 613 Aligned: 122 Modelled: 123 Confidence: 99.2% Identity: 11% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 1xrs chain B domain 1 Region: 475 - 613 Aligned: 135 Modelled: 139 Confidence: 98.9% Identity: 18% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 2yxb chain A Region: 491 - 613 Aligned: 122 Modelled: 123 Confidence: 98.8% Identity: 15% PDB header:isomerase Chain: A: PDB Molecule:coenzyme b12-dependent mutase; PDBTitle: crystal structure of the methylmalonyl-coa mutase alpha-subunit from2 aeropyrum pernix Phyre2 PDB 1xrs chain B Region: 475 - 613 Aligned: 135 Modelled: 139 Confidence: 98.7% Identity: 17% PDB header:isomerase Chain: B: PDB Molecule:d-lysine 5,6-aminomutase beta subunit; PDBTitle: crystal structure of lysine 5,6-aminomutase in complex with plp,2 cobalamin, and 5'-deoxyadenosine Phyre2 PDB 1fmf chain A Region: 491 - 615 Aligned: 124 Modelled: 125 Confidence: 98.6% Identity: 10% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 1y80 chain A Region: 490 - 609 Aligned: 119 Modelled: 120 Confidence: 98.6% Identity: 15% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:predicted cobalamin binding protein; PDBTitle: structure of a corrinoid (factor iiim)-binding protein from moorella2 thermoacetica Phyre2 PDB 4r3u chain D Region: 492 - 615 Aligned: 123 Modelled: 124 Confidence: 98.6% Identity: 19% PDB header:isomerase Chain: D: PDB Molecule:2-hydroxyisobutyryl-coa mutase small subunit; PDBTitle: crystal structure of 2-hydroxyisobutyryl-coa mutase Phyre2 PDB 4jgi chain B Region: 476 - 614 Aligned: 135 Modelled: 139 Confidence: 98.6% Identity: 19% PDB header:protein binding Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: 1.5 angstrom crystal structure of a novel cobalamin-binding protein2 from desulfitobacterium hafniense dcb-2 Phyre2 PDB 1bmt chain B Region: 473 - 610 Aligned: 137 Modelled: 138 Confidence: 98.5% Identity: 13% PDB header:methyltransferase Chain: B: PDB Molecule:methionine synthase; PDBTitle: how a protein binds b12: a 3.o angstrom x-ray structure of2 the b12-binding domains of methionine synthase Phyre2 PDB 3bul chain A domain 2 Region: 487 - 613 Aligned: 126 Modelled: 127 Confidence: 98.5% Identity: 13% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 2i2x chain D Region: 490 - 615 Aligned: 125 Modelled: 126 Confidence: 98.4% Identity: 10% PDB header:transferase Chain: D: PDB Molecule:methyltransferase 1; PDBTitle: crystal structure of methanol:cobalamin methyltransferase complex2 mtabc from methanosarcina barkeri Phyre2 1 c6reqB_ 2 c3bicA_ 3 c1e1cA_ 4 d7reqb1 5 d7reqa1 6 c4r3uB_ 7 c4xc8B_ 8 d7reqb2 9 d7reqa2 10 d1ccwa_ 11 d1xrsb1 12 c2yxbA_ 13 c1xrsB_ 14 d1fmfa_ 15 c1y80A_ 16 c4r3uD_ 17 c4jgiB_ 18 c1bmtB_ 19 d3bula2 20 c2i2xD_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288