1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	T	K	T	P	V	I	G	S	F	A	G	V	P	L	H	S	E	R	A	A	Q	S	P	T	E	A	A	V	H	T	H	V	A	A	A	A	A	A	H	G	Y	T	P	E	Q	L	V	W	H	T	P	E	G	I	D	V	T	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?		?			?	?	?	?	?	?	?	?	?														?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	Y	I	A	A	D	R	A	A	A	E	A	E	G	Y	P	L	H	S	F	P	G	E	P	P	F	V	R	G	P	Y	P	T	M	Y	V	N	Q	P	W	T	I	R	Q	Y	A	G	F	S	T	A	A	D	S	N	A	F	Y	R	R
Secondary structure
SS confidence
Disorder								?		?	?	?	?	?	?	?	?		?														?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		N	L	A	A	G	Q	K	G	L	S	V	A	F	D	L	A	T	H	R	G	Y	D	S	D	H	P	R	V	Q	G	D	V	G	M	A	G	V	A	I	D	S	I	L	D	M	R	Q	L	F	D	G	I	D	L	S	T	V	S	V	S
Secondary structure
SS confidence
Disorder																	?	?	?	?							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		M	T	M	N	G	A	V	L	P	I	L	A	L	Y	V	V	A	A	E	E	Q	G	V	A	P	E	Q	L	A	G	T	I	Q	N	D	I	L	K	E	F	M	V	R	N	T	Y	I	Y	P	P	K	P	S	M	R	I	I	S	D	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	A	Y	T	S	A	K	M	P	K	F	N	S	I	S	I	S	G	Y	H	I	Q	E	A	G	A	T	A	D	L	E	L	A	Y	T	L	A	D	G	V	D	Y	I	R	A	G	L	N	A	G	L	D	I	D	S	F	A	P	R	L
Secondary structure
SS confidence
Disorder																									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		S	F	F	W	G	I	G	M	N	F	F	M	E	V	A	K	L	R	A	G	R	L	L	W	S	E	L	V	A	Q	F	A	P	K	S	A	K	S	L	S	L	R	T	H	S	Q	T	S	G	W	S	L	T	A	Q	D	V	F	N	N
Secondary structure
SS confidence
Disorder																																																			?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		V	A	R	T	C	I	E	A	M	A	A	T	Q	G	H	T	Q	S	L	H	T	N	A	L	D	E	A	L	A	L	P	T	D	F	S	A	R	I	A	R	N	T	Q	L	V	L	Q	Q	E	S	G	T	T	R	P	I	D	P	W	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		G	S	Y	Y	V	E	W	L	T	H	R	L	A	R	R	A	R	A	H	I	A	E	V	A	E	H	G	G	M	A	Q	A	I	S	D	G	I	P	K	L	R	I	E	E	A	A	A	R	T	Q	A	R	I	D	S	G	Q	Q	P	V
Secondary structure
SS confidence
Disorder																																																							?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		V	G	V	N	K	Y	Q	V	P	E	D	H	E	I	E	V	L	K	V	E	N	S	R	V	R	A	E	Q	L	A	K	L	Q	R	L	R	A	G	R	D	E	P	A	V	R	A	A	L	A	E	L	T	R	A	A	A	E	Q	G	R
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?	?					?	?																?	?	?	?	?		?								?					?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		A	G	A	D	G	L	G	N	N	L	L	A	L	A	I	D	A	A	R	A	Q	A	T	V	G	E	I	S	E	A	L	E	K	V	Y	G	R	H	R	A	E	I	R	T	I	S	G	V	Y	R	D	E	V	G	K	A	P	N	I	A
Secondary structure
SS confidence
Disorder																									?																?					?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660
Sequence		A	A	T	E	L	V	E	K	F	A	E	A	D	G	R	R	P	R	I	L	I	A	K	M	G	Q	D	G	H	D	R	G	Q	K	V	I	A	T	A	F	A	D	I	G	F	D	V	D	V	G	S	L	F	S	T	P	E	E	V	A
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.	680	.	.	.	.	.	.	.	.	.	690	.	.	.	.	.	.	.	.	.	700	.	.	.	.	.	.	.	.	.	710	.	.	.	.	.	.	.	.	.	720
Sequence		R	Q	A	A	D	N	D	V	H	V	I	G	V	S	S	L	A	A	G	H	L	T	L	V	P	A	L	R	D	A	L	A	Q	V	G	R	P	D	I	M	I	V	V	G	G	V	I	P	P	G	D	F	D	E	L	Y	A	A	G	A
Secondary structure
SS confidence
Disorder																			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	730	.	.	.	.	.	.	.	.	.	740	.	.	.	.	.	.	.	.	.	750
Sequence		T	A	I	F	P	P	G	T	V	I	A	D	A	A	I	D	L	L	H	R	L	A	E	R	L	G	Y	T	L	D
Secondary structure
SS confidence
Disorder																										?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 13%) Alpha helix ( 50%) Beta strand ( 9%)

Rank Aligned region

PDB 1e1c chain A Region: 20 - 745 Aligned: 714 Modelled: 726 Confidence: 100.0% Identity: 71% PDB header:isomerase Chain: A: PDB Molecule:methylmalonyl-coa mutase alpha chain; PDBTitle: methylmalonyl-coa mutase h244a mutant Phyre2 PDB 3bic chain A Region: 15 - 745 Aligned: 701 Modelled: 719 Confidence: 100.0% Identity: 62% PDB header:isomerase Chain: A: PDB Molecule:methylmalonyl-coa mutase, mitochondrial precursor; PDBTitle: crystal structure of human methylmalonyl-coa mutase Phyre2 PDB 6req chain B Region: 14 - 748 Aligned: 603 Modelled: 631 Confidence: 100.0% Identity: 23% PDB header:isomerase Chain: B: PDB Molecule:protein (methylmalonyl-coa mutase); PDBTitle: methylmalonyl-coa mutase, 3-carboxypropyl-coa inhibitor complex Phyre2 PDB 7req chain A domain 1 Region: 15 - 578 Aligned: 548 Modelled: 564 Confidence: 100.0% Identity: 72% Fold: TIM beta/alpha-barrel Superfamily: Cobalamin (vitamin B12)-dependent enzymes Family: Methylmalonyl-CoA mutase, N-terminal (CoA-binding) domain Phyre2 PDB 4r3u chain B Region: 32 - 580 Aligned: 536 Modelled: 546 Confidence: 100.0% Identity: 41% PDB header:isomerase Chain: B: PDB Molecule:2-hydroxyisobutyryl-coa mutase large subunit; PDBTitle: crystal structure of 2-hydroxyisobutyryl-coa mutase Phyre2 PDB 4xc8 chain B Region: 48 - 579 Aligned: 512 Modelled: 529 Confidence: 100.0% Identity: 35% PDB header:isomerase Chain: B: PDB Molecule:isobutyryl-coa mutase fused; PDBTitle: isobutyryl-coa mutase fused with bound butyryl-coa, gdp, and mg and2 without cobalamin (apo-icmf/gdp) Phyre2 PDB 7req chain B domain 1 Region: 14 - 495 Aligned: 443 Modelled: 455 Confidence: 100.0% Identity: 27% Fold: TIM beta/alpha-barrel Superfamily: Cobalamin (vitamin B12)-dependent enzymes Family: Methylmalonyl-CoA mutase, N-terminal (CoA-binding) domain Phyre2 PDB 7req chain B domain 2 Region: 579 - 748 Aligned: 159 Modelled: 170 Confidence: 100.0% Identity: 14% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 7req chain A domain 2 Region: 580 - 745 Aligned: 166 Modelled: 166 Confidence: 100.0% Identity: 69% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 1ccw chain A Region: 615 - 748 Aligned: 130 Modelled: 134 Confidence: 99.6% Identity: 26% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 1xrs chain B Region: 599 - 745 Aligned: 142 Modelled: 147 Confidence: 99.4% Identity: 24% PDB header:isomerase Chain: B: PDB Molecule:d-lysine 5,6-aminomutase beta subunit; PDBTitle: crystal structure of lysine 5,6-aminomutase in complex with plp,2 cobalamin, and 5'-deoxyadenosine Phyre2 PDB 1xrs chain B domain 1 Region: 599 - 744 Aligned: 141 Modelled: 146 Confidence: 99.4% Identity: 25% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 2yxb chain A Region: 615 - 744 Aligned: 129 Modelled: 130 Confidence: 99.3% Identity: 47% PDB header:isomerase Chain: A: PDB Molecule:coenzyme b12-dependent mutase; PDBTitle: crystal structure of the methylmalonyl-coa mutase alpha-subunit from2 aeropyrum pernix Phyre2 PDB 1fmf chain A Region: 615 - 748 Aligned: 130 Modelled: 134 Confidence: 99.2% Identity: 28% Fold: Flavodoxin-like Superfamily: Cobalamin (vitamin B12)-binding domain Family: Cobalamin (vitamin B12)-binding domain Phyre2 PDB 3kox chain A Region: 98 - 743 Aligned: 597 Modelled: 636 Confidence: 99.2% Identity: 18% PDB header:metal binding protein Chain: A: PDB Molecule:d-ornithine aminomutase e component; PDBTitle: crystal structure of ornithine 4,5 aminomutase in complex with 2,4-2 diaminobutyrate (anaerobic) Phyre2 PDB 4r3u chain D Region: 616 - 743 Aligned: 127 Modelled: 128 Confidence: 99.1% Identity: 40% PDB header:isomerase Chain: D: PDB Molecule:2-hydroxyisobutyryl-coa mutase small subunit; PDBTitle: crystal structure of 2-hydroxyisobutyryl-coa mutase Phyre2 PDB 1bmt chain B Region: 514 - 742 Aligned: 210 Modelled: 215 Confidence: 99.0% Identity: 17% PDB header:methyltransferase Chain: B: PDB Molecule:methionine synthase; PDBTitle: how a protein binds b12: a 3.o angstrom x-ray structure of2 the b12-binding domains of methionine synthase Phyre2 PDB 4jgi chain B Region: 567 - 741 Aligned: 162 Modelled: 175 Confidence: 99.0% Identity: 21% PDB header:protein binding Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: 1.5 angstrom crystal structure of a novel cobalamin-binding protein2 from desulfitobacterium hafniense dcb-2 Phyre2 PDB 1y80 chain A Region: 615 - 738 Aligned: 122 Modelled: 124 Confidence: 98.9% Identity: 23% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:predicted cobalamin binding protein; PDBTitle: structure of a corrinoid (factor iiim)-binding protein from moorella2 thermoacetica Phyre2 PDB 3ezx chain A Region: 525 - 741 Aligned: 201 Modelled: 214 Confidence: 98.9% Identity: 18% PDB header:transferase Chain: A: PDB Molecule:monomethylamine corrinoid protein 1; PDBTitle: structure of methanosarcina barkeri monomethylamine2 corrinoid protein Phyre2 1 c1e1cA_ 2 c3bicA_ 3 c6reqB_ 4 d7reqa1 5 c4r3uB_ 6 c4xc8B_ 7 d7reqb1 8 d7reqb2 9 d7reqa2 10 d1ccwa_ 11 c1xrsB_ 12 d1xrsb1 13 c2yxbA_ 14 d1fmfa_ 15 c3koxA_ 16 c4r3uD_ 17 c1bmtB_ 18 c4jgiB_ 19 c1y80A_ 20 c3ezxA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499