1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	S	N	N	A	G	E	S	D	G	T	G	P	A	L	P	T	V	W	P	G	N	A	Y	P	L	G	A	T	Y	D	G	A	G	T	N	F	S	L	F	S	E	I	A	E	K	V	E	L	C	L	I	D	E	D	G	V	E	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	I	P	L	D	E	V	D	G	Y	V	W	H	A	Y	L	P	N	I	T	P	G	Q	R	Y	G	F	R	V	H	G	P	F	D	P	A	A	G	H	R	C	D	P	S	K	L	L	L	D	P	Y	G	K	S	F	H	G	D	F	T
Secondary structure
SS confidence
Disorder																																																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		F	G	Q	A	L	Y	S	Y	D	V	N	A	V	D	P	D	S	T	P	P	M	V	D	S	L	G	H	T	M	T	S	V	V	I	N	P	F	F	D	W	A	Y	D	R	S	P	R	T	P	Y	H	E	T	V	I	Y	E	A	H	V
Secondary structure
SS confidence
Disorder																			?				?	?	?	?	?																		?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		K	G	M	T	Q	T	H	P	S	I	P	P	E	L	R	G	T	Y	A	G	L	A	H	P	V	I	I	D	H	L	N	E	L	N	V	T	A	V	E	L	M	P	V	H	Q	F	L	H	D	S	R	L	L	D	L	G	L	R	N	Y
Secondary structure
SS confidence
Disorder						?	?	?	?	?	?	?	?																																					?	?	?	?	?	?	?		?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		W	G	Y	N	T	F	G	F	F	A	P	H	H	Q	Y	A	S	T	R	Q	A	G	S	A	V	A	E	F	K	T	M	V	R	S	L	H	E	A	G	I	E	V	I	L	D	V	V	Y	N	H	T	A	E	G	N	H	L	G	P	T
Secondary structure
SS confidence
Disorder																?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		I	N	F	R	G	I	D	N	T	A	Y	Y	R	L	M	D	H	D	L	R	F	Y	K	D	F	T	G	T	G	N	S	L	N	A	R	H	P	H	T	L	Q	L	I	M	D	S	L	R	Y	W	V	I	E	M	H	V	D	G	F	R
Secondary structure
SS confidence
Disorder																		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		F	D	L	A	S	T	L	A	R	E	L	H	D	V	D	R	L	S	A	F	F	D	L	V	Q	Q	D	P	V	V	S	Q	V	K	L	I	A	E	P	W	D	V	G	E	G	G	Y	Q	V	G	N	F	P	G	L	W	T	E	W	N
Secondary structure
SS confidence
Disorder																																														?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		G	K	Y	R	D	T	V	R	D	Y	W	R	G	E	P	A	T	L	G	E	F	A	S	R	L	T	G	S	S	D	L	Y	E	A	T	G	R	R	P	S	A	S	I	N	F	V	T	A	H	D	G	F	T	L	N	D	L	V	S	Y
Secondary structure
SS confidence
Disorder																?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		N	D	K	H	N	E	A	N	G	E	N	N	R	D	G	E	S	Y	N	R	S	W	N	C	G	V	E	G	P	T	D	D	P	D	I	L	A	L	R	A	R	Q	M	R	N	M	W	A	T	L	M	V	S	Q	G	T	P	M	I	A
Secondary structure
SS confidence
Disorder											?	?	?	?	?	?	?											?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		H	G	D	E	I	G	R	T	Q	Y	G	N	N	N	V	Y	C	Q	D	S	E	L	S	W	M	D	W	S	L	V	D	K	N	A	D	L	L	A	F	A	R	K	A	T	T	L	R	K	N	H	K	V	F	R	R	R	R	F	F	E
Secondary structure
SS confidence
Disorder														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660
Sequence		G	E	P	I	R	S	G	D	E	V	R	D	I	A	W	L	T	P	S	G	R	E	M	T	H	E	D	W	G	R	G	F	D	R	C	V	A	V	F	L	N	G	E	A	I	T	A	P	D	A	R	G	E	R	V	V	D	D	S	F
Secondary structure
SS confidence
Disorder					?	?	?																				?	?																	?	?	?	?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.	680	.	.	.	.	.	.	.	.	.	690	.	.	.	.	.	.	.	.	.	700	.	.	.	.	.	.	.	.	.	710	.	.	.	.	.	.	.	.	.	720
Sequence		L	L	C	F	N	A	H	D	H	D	V	E	F	V	M	P	H	D	G	Y	A	Q	Q	W	T	G	E	L	D	T	N	D	P	V	G	D	I	D	L	T	V	T	A	T	D	T	F	S	V	P	A	R	S	L	L	V	L	R	K	T
Secondary structure
SS confidence
Disorder																																		?	?	?	?	?	?	?	?	?	?	?	?	?													?	?	?
Disorder confidence
		.
Sequence		L
Secondary structure
SS confidence
Disorder		?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 15%) Alpha helix ( 21%) Beta strand ( 18%) TM helix ( 3%) (View membrane topology prediction)

Rank Aligned region

PDB 4j7r chain A Region: 6 - 720 Aligned: 679 Modelled: 698 Confidence: 100.0% Identity: 41% PDB header:hydrolase Chain: A: PDB Molecule:isoamylase; PDBTitle: crystal structure of chlamydomonas reinhardtii isoamylase 1 (isa1) Phyre2 PDB 2vnc chain B Region: 18 - 719 Aligned: 652 Modelled: 652 Confidence: 100.0% Identity: 51% PDB header:hydrolase Chain: B: PDB Molecule:glycogen operon protein glgx; PDBTitle: crystal structure of glycogen debranching enzyme trex from2 sulfolobus solfataricus Phyre2 PDB 2wsk chain A Region: 19 - 719 Aligned: 652 Modelled: 679 Confidence: 100.0% Identity: 46% PDB header:hydrolase Chain: A: PDB Molecule:glycogen debranching enzyme; PDBTitle: crystal structure of glycogen debranching enzyme glgx from2 escherichia coli k-12 Phyre2 PDB 1bf2 chain A Region: 23 - 719 Aligned: 669 Modelled: 688 Confidence: 100.0% Identity: 35% PDB header:hydrolase Chain: A: PDB Molecule:isoamylase; PDBTitle: structure of pseudomonas isoamylase Phyre2 PDB 2x4b chain A Region: 12 - 719 Aligned: 627 Modelled: 640 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:limit dextrinase; PDBTitle: barley limit dextrinase in complex with beta-cyclodextrin Phyre2 PDB 2fhf chain A Region: 20 - 719 Aligned: 622 Modelled: 637 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:pullulanase; PDBTitle: crystal structure analysis of klebsiella pneumoniae pullulanase2 complexed with maltotetraose Phyre2 PDB 3wdj chain A Region: 20 - 719 Aligned: 572 Modelled: 606 Confidence: 100.0% Identity: 28% PDB header:hydrolase Chain: A: PDB Molecule:type i pullulanase; PDBTitle: crystal structure of pullulanase complexed with maltotetraose from2 anoxybacillus sp. lm18-11 Phyre2 PDB 2e8y chain A Region: 20 - 720 Aligned: 582 Modelled: 618 Confidence: 100.0% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:amyx protein; PDBTitle: crystal structure of pullulanase type i from bacillus subtilis str.2 168 Phyre2 PDB 5wvt chain A Region: 14 - 719 Aligned: 589 Modelled: 630 Confidence: 100.0% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:pulullanase; PDBTitle: catalytic mechanism, cyclodextrin inhibition, and allosteric2 regulation of paenibacillus barengoltzii pulllulanase Phyre2 PDB 3fax chain A Region: 26 - 720 Aligned: 595 Modelled: 624 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:reticulocyte binding protein; PDBTitle: the crystal structure of gbs pullulanase sap in complex with2 maltotetraose Phyre2 PDB 2ya1 chain A Region: 26 - 720 Aligned: 601 Modelled: 654 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:putative alkaline amylopullulanase; PDBTitle: product complex of a multi-modular glycogen-degrading pneumococcal2 virulence factor spua Phyre2 PDB 2wan chain A Region: 26 - 719 Aligned: 574 Modelled: 610 Confidence: 100.0% Identity: 28% PDB header:hydrolase Chain: A: PDB Molecule:pullulanase; PDBTitle: pullulanase from bacillus acidopullulyticus Phyre2 PDB 2ya0 chain A Region: 20 - 720 Aligned: 603 Modelled: 645 Confidence: 100.0% Identity: 25% PDB header:hydrolase Chain: A: PDB Molecule:putative alkaline amylopullulanase; PDBTitle: catalytic module of the multi-modular glycogen-degrading pneumococcal2 virulence factor spua Phyre2 PDB 5gqu chain A Region: 27 - 720 Aligned: 557 Modelled: 586 Confidence: 100.0% Identity: 19% PDB header:transferase Chain: A: PDB Molecule:1,4-alpha-glucan branching enzyme glgb; PDBTitle: crystal structure of branching enzyme from cyanothece sp. atcc 51142 Phyre2 PDB 3k1d chain A Region: 27 - 718 Aligned: 547 Modelled: 592 Confidence: 100.0% Identity: 23% PDB header:transferase Chain: A: PDB Molecule:1,4-alpha-glucan-branching enzyme; PDBTitle: crystal structure of glycogen branching enzyme synonym: 1,4-alpha-d-2 glucan:1,4-alpha-d-glucan 6-glucosyl-transferase from mycobacterium3 tuberculosis h37rv Phyre2 PDB 3amk chain A Region: 27 - 719 Aligned: 568 Modelled: 607 Confidence: 100.0% Identity: 19% PDB header:transferase Chain: A: PDB Molecule:os06g0726400 protein; PDBTitle: structure of the starch branching enzyme i (bei) from oryza sativa l Phyre2 PDB 3aml chain A Region: 27 - 720 Aligned: 575 Modelled: 602 Confidence: 100.0% Identity: 18% PDB header:transferase Chain: A: PDB Molecule:os06g0726400 protein; PDBTitle: structure of the starch branching enzyme i (bei) from oryza sativa l Phyre2 PDB 4bzy chain C Region: 27 - 719 Aligned: 569 Modelled: 607 Confidence: 100.0% Identity: 18% PDB header:transferase Chain: C: PDB Molecule:1,4-alpha-glucan-branching enzyme; PDBTitle: crystal structure of human glycogen branching enzyme (gbe1) Phyre2 PDB 1m7x chain C Region: 27 - 719 Aligned: 541 Modelled: 587 Confidence: 100.0% Identity: 19% PDB header:transferase Chain: C: PDB Molecule:1,4-alpha-glucan branching enzyme; PDBTitle: the x-ray crystallographic structure of branching enzyme Phyre2 PDB 3m07 chain A Region: 26 - 717 Aligned: 520 Modelled: 568 Confidence: 100.0% Identity: 26% PDB header:unknown function Chain: A: PDB Molecule:putative alpha amylase; PDBTitle: 1.4 angstrom resolution crystal structure of putative alpha amylase2 from salmonella typhimurium. Phyre2 1 c4j7rA_ 2 c2vncB_ 3 c2wskA_ 4 c1bf2A_ 5 c2x4bA_ 6 c2fhfA_ 7 c3wdjA_ 8 c2e8yA_ 9 c5wvtA_ 10 c3faxA_ 11 c2ya1A_ 12 c2wanA_ 13 c2ya0A_ 14 c5gquA_ 15 c3k1dA_ 16 c3amkA_ 17 c3amlA_ 18 c4bzyC_ 19 c1m7xC_ 20 c3m07A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499