1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	T	L	S	P	P	R	P	P	A	L	T	P	E	P	A	L	P	P	V	T	M	G	T	R	T	T	G	F	Y	R	H	D	L	D	G	L	R	G	V	A	I	A	L	V	A	V	F	H	V	W	F	G	R	V	S	G	G	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	V	F	L	A	L	S	G	F	F	F	G	G	K	I	L	R	A	A	L	N	P	D	L	S	L	S	P	I	A	E	V	I	R	L	I	R	R	L	L	P	A	L	V	V	V	L	A	G	C	A	L	L	T	I	A	I	Q	P	Q
Secondary structure
SS confidence
Disorder																						?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	R	W	E	A	F	A	N	Q	S	L	A	S	L	G	Y	Y	Q	N	W	E	L	A	S	T	V	S	N	Y	L	R	A	G	E	A	V	S	P	L	Q	H	I	W	S	M	S	V	Q	G	Q	F	Y	L	A	F	L	L	L	V	A
Secondary structure
SS confidence
Disorder																												?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	C	A	Y	L	L	R	R	L	F	R	G	P	R	A	P	Y	L	R	T	M	F	V	V	L	L	S	T	L	T	L	A	S	F	I	Y	A	I	V	A	H	H	A	Y	Q	A	T	A	Y	Y	N	T	F	A	R	A	W	E	L	L
Secondary structure
SS confidence
Disorder								?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	G	A	L	V	G	A	V	V	P	H	V	R	W	P	M	W	L	R	T	A	V	A	T	A	A	L	A	A	I	L	S	C	G	A	L	I	D	G	V	K	E	F	P	G	P	W	A	L	V	P	V	G	A	T	M	L	M	I	L
Secondary structure
SS confidence
Disorder													?	?	?	?	?																								?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	G	A	N	R	Q	G	H	P	G	T	R	D	R	L	P	L	P	N	R	L	L	A	T	A	P	L	V	A	L	G	A	M	A	Y	S	W	Y	L	W	H	W	P	L	L	I	F	W	L	S	Y	T	G	H	R	H	A	N	F	V
Secondary structure
SS confidence
Disorder						?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		E	G	A	A	V	L	L	V	S	G	L	L	A	Y	L	T	T	R	L	V	E	D	P	L	R	Y	R	A	P	A	G	V	R	S	P	A	A	V	P	P	I	P	W	R	L	R	L	R	R	P	T	I	V	L	G	S	V	V	A	L
Secondary structure
SS confidence
Disorder																												?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		L	G	V	A	L	T	A	T	S	F	T	W	R	E	H	V	I	V	Q	R	A	A	G	K	E	L	S	G	L	S	S	R	D	Y	P	G	A	R	A	L	I	D	H	V	R	V	P	K	L	R	M	R	P	T	V	L	E	V	R	H
Secondary structure
SS confidence
Disorder											?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?				?	?					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		D	L	P	T	S	T	K	D	G	C	I	S	D	F	V	N	P	A	I	I	N	C	T	Y	G	D	V	D	A	P	R	T	I	A	L	A	G	G	S	H	A	E	H	W	L	T	A	L	D	L	L	G	R	M	H	H	F	K	V	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		T	Y	L	K	M	G	C	P	L	S	T	E	E	V	P	L	I	M	G	N	N	A	P	Y	P	Q	C	H	Q	W	V	Q	A	A	M	A	K	L	V	A	D	H	P	D	Y	V	F	T	T	S	T	R	P	W	N	I	K	P	G	D
Secondary structure
SS confidence
Disorder									?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																												?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660
Sequence		V	M	P	A	T	Y	V	G	I	W	Q	T	F	A	D	N	N	I	P	V	L	A	M	R	D	T	P	W	L	V	K	D	G	Q	P	F	I	P	A	D	C	L	A	K	G	G	N	P	Q	S	C	G	I	A	R	S	K	V	L	V
Secondary structure
SS confidence
Disorder																													?															?	?					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.	680	.	.	.	.	.	.	.	.	.	690	.	.	.	.	.	.	.	.	.	700	.	.	.	.	.	.	.	.	.	710	.	.	.	.	.	.	.	.	.	720
Sequence		D	R	N	P	T	L	D	F	V	A	R	F	P	L	L	K	P	L	D	M	S	D	A	I	C	R	T	D	T	C	R	A	V	E	G	N	V	L	V	Y	R	D	S	H	H	L	T	P	T	Y	M	R	T	M	T	S	E	L	G	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.
Sequence		Q	I	A	A	N	T	D	W	W
Secondary structure
SS confidence
Disorder						?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 20%) Alpha helix ( 58%) Beta strand ( 5%) TM helix ( 31%) (View membrane topology prediction)

Rank Aligned region

PDB 4h08 chain A Region: 506 - 726 Aligned: 183 Modelled: 203 Confidence: 99.1% Identity: 8% PDB header:hydrolase Chain: A: PDB Molecule:putative hydrolase; PDBTitle: crystal structure of a putative hydrolase (bt3161) from bacteroides2 thetaiotaomicron vpi-5482 at 1.80 a resolution Phyre2 PDB 4iyj chain A Region: 506 - 726 Aligned: 183 Modelled: 188 Confidence: 99.1% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:gdsl-like protein; PDBTitle: crystal structure of a putative acylhydrolase (bacuni_03406) from2 bacteroides uniformis atcc 8492 at 1.37 a resolution Phyre2 PDB 3p94 chain A Region: 505 - 726 Aligned: 182 Modelled: 188 Confidence: 99.0% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:gdsl-like lipase; PDBTitle: crystal structure of a gdsl-like lipase (bdi_0976) from2 parabacteroides distasonis atcc 8503 at 1.93 a resolution Phyre2 PDB 4rsh chain B Region: 510 - 722 Aligned: 168 Modelled: 169 Confidence: 99.0% Identity: 13% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:lipolytic protein g-d-s-l family; PDBTitle: structure of a putative lipolytic protein of g-d-s-l family from2 desulfitobacterium hafniense dcb-2 Phyre2 PDB 1es9 chain A Region: 508 - 726 Aligned: 179 Modelled: 183 Confidence: 99.0% Identity: 15% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Acetylhydrolase Phyre2 PDB 2o14 chain A Region: 506 - 725 Aligned: 185 Modelled: 185 Confidence: 98.9% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein yxim; PDBTitle: x-ray crystal structure of protein yxim_bacsu from bacillus subtilis.2 northeast structural genomics consortium target sr595 Phyre2 PDB 4jgg chain B Region: 511 - 726 Aligned: 170 Modelled: 177 Confidence: 98.9% Identity: 12% PDB header:hydrolase Chain: B: PDB Molecule:esterase tesa; PDBTitle: crystal structure of tesa Phyre2 PDB 1yzf chain A domain 1 Region: 511 - 728 Aligned: 180 Modelled: 185 Confidence: 98.9% Identity: 16% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: TAP-like Phyre2 PDB 6iqb chain A Region: 508 - 726 Aligned: 175 Modelled: 182 Confidence: 98.8% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:sgnh-hydrolase family esterase; PDBTitle: esterase aline4 mutant-s13a Phyre2 PDB 1fxw chain F Region: 507 - 726 Aligned: 180 Modelled: 184 Confidence: 98.8% Identity: 14% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: Acetylhydrolase Phyre2 PDB 3mil chain A Region: 511 - 729 Aligned: 189 Modelled: 201 Confidence: 98.8% Identity: 11% PDB header:hydrolase Chain: A: PDB Molecule:isoamyl acetate-hydrolyzing esterase; PDBTitle: crystal structure of isoamyl acetate-hydrolyzing esterase from2 saccharomyces cerevisiae Phyre2 PDB 5ufy chain A Region: 508 - 727 Aligned: 166 Modelled: 177 Confidence: 98.8% Identity: 13% PDB header:transferase Chain: A: PDB Molecule:acyltransferase; PDBTitle: structure of streptococcus pneumoniae peptidoglycan o-2 acetyltransferase a (oata) c-terminal catalytic domain Phyre2 PDB 4s1p chain A Region: 509 - 722 Aligned: 183 Modelled: 213 Confidence: 98.8% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: shel_16390 protein, a putative sgnh hydrolase from slackia2 heliotrinireducens Phyre2 PDB 1jrl chain A Region: 510 - 727 Aligned: 168 Modelled: 177 Confidence: 98.8% Identity: 17% Fold: Flavodoxin-like Superfamily: SGNH hydrolase Family: TAP-like Phyre2 PDB 5jd3 chain A Region: 508 - 722 Aligned: 175 Modelled: 179 Confidence: 98.7% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:lae5; PDBTitle: crystal structure of lae5, an alpha/beta hydrolase enzyme from the2 metagenome of lake arreo, spain Phyre2 PDB 6njc chain B Region: 507 - 722 Aligned: 176 Modelled: 182 Confidence: 98.7% Identity: 14% PDB header:hydrolase Chain: B: PDB Molecule:sialate o-acetylesterase; PDBTitle: crystal structure of the sialate o-acetylesterase from bacteroides2 vulgatus Phyre2 PDB 6iq8 chain B Region: 508 - 727 Aligned: 176 Modelled: 183 Confidence: 98.7% Identity: 14% PDB header:hydrolase Chain: B: PDB Molecule:carboxylesterase; PDBTitle: esterase crme10 mutant-d178a Phyre2 PDB 3hp4 chain A Region: 510 - 726 Aligned: 171 Modelled: 178 Confidence: 98.7% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:gdsl-esterase; PDBTitle: crystal structure of psychrotrophic esterase esta from2 pseudoalteromonas sp. 643a inhibited by monoethylphosphonate Phyre2 PDB 2vpt chain A Region: 509 - 727 Aligned: 189 Modelled: 199 Confidence: 98.6% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:lipolytic enzyme; PDBTitle: clostridium thermocellum family 3 carbohydrate esterase Phyre2 PDB 3rjt chain A Region: 505 - 722 Aligned: 178 Modelled: 181 Confidence: 98.6% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:lipolytic protein g-d-s-l family; PDBTitle: crystal structure of lipolytic protein g-d-s-l family from2 alicyclobacillus acidocaldarius subsp. acidocaldarius dsm 446 Phyre2 1 c4h08A_ 2 c4iyjA_ 3 c3p94A_ 4 c4rshB_ 5 d1es9a_ 6 c2o14A_ 7 c4jggB_ 8 d1yzfa1 9 c6iqbA_ 10 d1fxwf_ 11 c3milA_ 12 c5ufyA_ 13 c4s1pA_ 14 d1jrla_ 15 c5jd3A_ 16 c6njcB_ 17 c6iq8B_ 18 c3hp4A_ 19 c2vptA_ 20 c3rjtA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185