1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	P	D	G	K	P	M	S	D	F	D	E	L	L	A	V	L	D	L	N	A	V	A	S	D	L	F	T	G	S	H	P	S	K	N	P	L	R	T	F	G	G	Q	L	M	A	Q	S	F	V	A	S	S	R	T	L	T	R	H	H
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?		?																									?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	P	P	S	A	F	S	V	H	F	I	N	G	G	D	T	A	K	D	I	E	F	Q	V	I	R	L	R	D	E	R	R	F	A	N	R	R	V	D	A	V	Q	D	G	T	L	L	S	S	A	M	V	S	Y	M	A	G	G	R	G
Secondary structure
SS confidence
Disorder																																																								?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	E	H	A	L	D	P	P	Q	V	A	E	P	H	T	R	P	P	I	G	E	L	L	R	G	Y	E	E	T	V	P	H	F	V	N	A	L	Q	P	I	E	W	R	Y	A	N	D	P	A	W	I	M	R	D	K	G	D	R	L	A
Secondary structure
SS confidence
Disorder		?	?	?																				?																									?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Y	N	R	V	W	V	K	A	L	G	E	M	P	D	D	P	V	L	H	T	A	T	L	L	Y	S	S	D	T	T	V	L	D	S	V	I	T	T	H	G	L	S	W	G	F	D	R	I	F	A	A	S	A	N	H	S	V	W	F	H
Secondary structure
SS confidence
Disorder																																	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		R	Q	V	N	F	D	D	W	V	L	Y	S	T	S	S	P	V	A	A	D	S	R	G	L	G	S	G	H	F	F	D	R	S	G	K	L	I	A	T	V	V	Q	E	G	V	L	K	Y	F	P	A	T	P	D	S	A	A	G	R	S
Secondary structure
SS confidence
Disorder																																																	?		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
Sequence
Secondary structure
SS confidence
Disorder
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 20%) Alpha helix ( 19%) Beta strand ( 37%) TM helix ( 11%) (View membrane topology prediction)

Rank Aligned region

PDB 1c8u chain A Region: 9 - 290 Aligned: 279 Modelled: 282 Confidence: 100.0% Identity: 30% PDB header:hydrolase Chain: A: PDB Molecule:acyl-coa thioesterase ii; PDBTitle: crystal structure of the e.coli thioesterase ii, a2 homologue of the human nef-binding enzyme Phyre2 PDB 3rd7 chain A Region: 7 - 287 Aligned: 272 Modelled: 281 Confidence: 100.0% Identity: 30% PDB header:hydrolase Chain: A: PDB Molecule:acyl-coa thioesterase; PDBTitle: crystal structure of acyl-coa thioesterase from mycobacterium avium Phyre2 PDB 4r9z chain B Region: 8 - 287 Aligned: 247 Modelled: 262 Confidence: 100.0% Identity: 32% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: mycobacterium avium subs paratuberculosis tesb protein map1729c Phyre2 PDB 3bbj chain A Region: 12 - 286 Aligned: 259 Modelled: 267 Confidence: 100.0% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:putative thioesterase ii; PDBTitle: crystal structure of a putative thioesterase ii (tfu_2367) from2 thermobifida fusca yx at 2.45 a resolution Phyre2 PDB 3rqb chain B Region: 11 - 287 Aligned: 251 Modelled: 272 Confidence: 100.0% Identity: 20% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of conserved protein of unknown function with hot2 dog fold from alicyclobacillus acidocaldarius Phyre2 PDB 3cjy chain A Region: 15 - 287 Aligned: 245 Modelled: 264 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:putative thioesterase; PDBTitle: crystal structure of putative thioesterase (yp_496845.1) from2 novosphingobium aromaticivorans dsm 12444 at 1.70 a resolution Phyre2 PDB 1c8u chain A domain 2 Region: 122 - 290 Aligned: 167 Modelled: 169 Confidence: 100.0% Identity: 31% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: Acyl-CoA thioesterase Phyre2 PDB 1c8u chain A domain 1 Region: 8 - 121 Aligned: 113 Modelled: 114 Confidence: 100.0% Identity: 30% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: Acyl-CoA thioesterase Phyre2 PDB 1tbu chain A domain 1 Region: 15 - 115 Aligned: 95 Modelled: 101 Confidence: 100.0% Identity: 33% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: Acyl-CoA thioesterase Phyre2 PDB 3b7k chain A Region: 34 - 281 Aligned: 219 Modelled: 226 Confidence: 99.2% Identity: 11% PDB header:hydrolase Chain: A: PDB Molecule:acyl-coenzyme a thioesterase 12; PDBTitle: human acyl-coenzyme a thioesterase 12 Phyre2 PDB 2gvh chain C Region: 37 - 281 Aligned: 197 Modelled: 203 Confidence: 99.0% Identity: 14% PDB header:hydrolase Chain: C: PDB Molecule:agr_l_2016p; PDBTitle: crystal structure of acyl-coa hydrolase (15159470) from agrobacterium2 tumefaciens at 2.65 a resolution Phyre2 PDB 2pim chain A Region: 5 - 115 Aligned: 110 Modelled: 109 Confidence: 99.0% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:phenylacetic acid degradation-related protein; PDBTitle: crystal structure of a putative thioesterase, phenylacetic acid2 degradation-related protein (reut_b4779) from ralstonia eutropha3 jmp134 at 2.20 a resolution Phyre2 PDB 4zv3 chain B Region: 34 - 281 Aligned: 227 Modelled: 248 Confidence: 99.0% Identity: 11% PDB header:hydrolase Chain: B: PDB Molecule:cytosolic acyl coenzyme a thioester hydrolase; PDBTitle: crystal structure of the n- and c-terminal domains of mouse acyl-coa2 thioesterase 7 Phyre2 PDB 1wlu chain A domain 1 Region: 11 - 116 Aligned: 106 Modelled: 106 Confidence: 99.0% Identity: 19% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 1zki chain A domain 1 Region: 2 - 116 Aligned: 113 Modelled: 115 Confidence: 98.9% Identity: 17% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 2qwz chain B Region: 1 - 117 Aligned: 117 Modelled: 116 Confidence: 98.9% Identity: 15% PDB header:hydrolase Chain: B: PDB Molecule:phenylacetic acid degradation-related protein; PDBTitle: crystal structure of a putative thioesterase (tm1040_1390) from2 silicibacter sp. tm1040 at 2.15 a resolution Phyre2 PDB 2hbo chain A domain 1 Region: 6 - 127 Aligned: 122 Modelled: 122 Confidence: 98.8% Identity: 10% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 2f0x chain A domain 1 Region: 8 - 114 Aligned: 107 Modelled: 107 Confidence: 98.7% Identity: 16% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 2h4u chain A domain 1 Region: 9 - 114 Aligned: 106 Modelled: 106 Confidence: 98.7% Identity: 15% Fold: Thioesterase/thiol ester dehydrase-isomerase Superfamily: Thioesterase/thiol ester dehydrase-isomerase Family: PaaI/YdiI-like Phyre2 PDB 3lwg chain B Region: 17 - 121 Aligned: 105 Modelled: 105 Confidence: 98.7% Identity: 12% PDB header:unknown function Chain: B: PDB Molecule:hp0420 homologue; PDBTitle: crystal structure of hp0420-homologue c46a from helicobacter felis Phyre2 1 c1c8uA_ 2 c3rd7A_ 3 c4r9zB_ 4 c3bbjA_ 5 c3rqbB_ 6 c3cjyA_ 7 d1c8ua2 8 d1c8ua1 9 d1tbua1 10 c3b7kA_ 11 c2gvhC_ 12 c2pimA_ 13 c4zv3B_ 14 d1wlua1 15 d1zkia1 16 c2qwzB_ 17 d2hboa1 18 d2f0xa1 19 d2h4ua1 20 c3lwgB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188