Phyre Home Retrieve Phyre Job Id
Emailmdejesus@rockefeller.edu
DescriptionRVBD1625c_(cya)_1826621_1827952
DateFri Aug 2 13:30:21 BST 2019
Unique Job ID4ee589b5a66987da
SequenceVAARKCGAPP ... Download FASTA
Job Typenormal
Job Expiry

Summary 

Top model
Image coloured by rainbow N → C terminus
Model dimensions (Å):X:125.414 Y:61.848 Z:87.123
Model (left) based on template c6r4oA_
Top template information
PDB header:membrane protein
Chain: A: PDB Molecule:adenylate cyclase 9;
PDBTitle: structure of a truncated adenylyl cyclase bound to mant-gtp, forskolin2 and an activated stimulatory galphas protein
Confidence and coverage
Confidence:100.0% Coverage: 88%
389 residues ( 88% of your sequence) have been modelled with 100.0% confidence by the single highest scoring template.
3D viewing
Interactive 3D view in JSmol
For other options to view your downloaded structure offline see the FAQ

Sequence analysis 

Download FASTA version

Secondary structure and disorder prediction [Show] 

Domain analysis [Show] 

Detailed template information [Hide] 

 

#
Template Alignment Coverage3D Model Confidence
% i.d. Template Information
1c6r4oA_



100.0 21
PDB header:membrane protein
Chain: A: PDB Molecule:adenylate cyclase 9;
PDBTitle: structure of a truncated adenylyl cyclase bound to mant-gtp, forskolin2 and an activated stimulatory galphas protein
2c6fhtB_



100.0 24
PDB header:lyase
Chain: B: PDB Molecule:bacteriophytochrome,adenylate cyclase;
PDBTitle: crystal structure of an artificial phytochrome regulated2 adenylate/guanylate cyclase in its dark adapted pr form
3c6r4pA_



100.0 36
PDB header:membrane protein
Chain: A: PDB Molecule:adenylate cyclase 9;
PDBTitle: structure of a soluble domain of adenylyl cyclase bound to an2 activated stimulatory g protein
4c5o5kC_



100.0 85
PDB header:membrane protein
Chain: C: PDB Molecule:adenylate cyclase;
PDBTitle: x-ray structure of a bacterial adenylyl cyclase soluble domain
5c4yusA_



100.0 25
PDB header:lyase
Chain: A: PDB Molecule:family 3 adenylate cyclase;
PDBTitle: crystal structure of photoactivated adenylyl cyclase of a2 cyanobacteriaoscillatoria acuminata in hexagonal form
6c5nbyA_



100.0 20
PDB header:lyase
Chain: A: PDB Molecule:beta subunit of photoactivated adenylyl cyclase;
PDBTitle: structure of a bacterial light-regulated adenylyl cylcase
7c4cllA_



100.0 20
PDB header:lyase
Chain: A: PDB Molecule:adenylate cyclase type 10;
PDBTitle: crystal structure of human soluble adenylyl cyclase in complex with2 bicarbonate
8c1cjkA_



100.0 33
PDB header:lyase/lyase/signaling protein
Chain: A: PDB Molecule:adenylate cyclase, type v;
PDBTitle: complex of gs-alpha with the catalytic domains of mammalian adenylyl2 cyclase: complex with adenosine 5'-(alpha thio)-triphosphate (rp),3 mg, and mn
9d1azsa_



100.0 33
Fold:Ferredoxin-like
Superfamily:Nucleotide cyclase
Family:Adenylyl and guanylyl cyclase catalytic domain
10d1azsb_



100.0 37
Fold:Ferredoxin-like
Superfamily:Nucleotide cyclase
Family:Adenylyl and guanylyl cyclase catalytic domain
11c1y10C_



100.0 27
PDB header:lyase
Chain: C: PDB Molecule:hypothetical protein rv1264/mt1302;
PDBTitle: mycobacterial adenylyl cyclase rv1264, holoenzyme, inhibited state
12c2w01C_



100.0 24
PDB header:lyase
Chain: C: PDB Molecule:adenylate cyclase;
PDBTitle: crystal structure of the guanylyl cyclase cya2
13c1yk9A_



100.0 98
PDB header:lyase
Chain: A: PDB Molecule:adenylate cyclase;
PDBTitle: crystal structure of a mutant form of the mycobacterial2 adenylyl cyclase rv1625c
14c1wc6B_



100.0 25
PDB header:lyase
Chain: B: PDB Molecule:adenylate cyclase;
PDBTitle: soluble adenylyl cyclase cyac from s. platensis in complex2 with rp-atpalphas in presence of bicarbonate
15d1wc1a_



100.0 25
Fold:Ferredoxin-like
Superfamily:Nucleotide cyclase
Family:Adenylyl and guanylyl cyclase catalytic domain
16c3r5gB_



100.0 28
PDB header:lyase
Chain: B: PDB Molecule:cyab;
PDBTitle: crystal structure of the adenylyl cyclase cyab from p. aeruginosa
17c3uvjC_



100.0 32
PDB header:lyase
Chain: C: PDB Molecule:guanylate cyclase soluble subunit alpha-3;
PDBTitle: crystal structure of the catalytic domain of the heterodimeric human2 soluble guanylate cyclase 1.
18c6aoaA_



100.0 36
PDB header:lyase
Chain: A: PDB Molecule:bacterio-rhodopsin/guanylyl cyclase 1 fusion protein;
PDBTitle: monomeric crystal structure of the e497/c566d double mutant of the2 guanylyl cyclase domain of the rhogc fusion protein from the aquatic3 fungus blastocladiella emersonii
19d1fx2a_



100.0 22
Fold:Ferredoxin-like
Superfamily:Nucleotide cyclase
Family:Adenylyl and guanylyl cyclase catalytic domain
20c2wz1B_



100.0 39
PDB header:lyase
Chain: B: PDB Molecule:guanylate cyclase soluble subunit beta-1;
PDBTitle: structure of the catalytic domain of human soluble guanylate cyclase 12 beta 3.
21d1fx4a_



not modelled 100.0 20
Fold:Ferredoxin-like
Superfamily:Nucleotide cyclase
Family:Adenylyl and guanylyl cyclase catalytic domain
22c3et6A_



not modelled 100.0 50
PDB header:lyase
Chain: A: PDB Molecule:soluble guanylyl cyclase beta;
PDBTitle: the crystal structure of the catalytic domain of a eukaryotic2 guanylate cyclase
23c3mr7B_



not modelled 99.9 27
PDB header:hydrolase
Chain: B: PDB Molecule:adenylate/guanylate cyclase/hydrolase, alpha/beta fold
PDBTitle: crystal structure of adenylate/guanylate cyclase/hydrolase from2 silicibacter pomeroyi
24c1ybuA_



not modelled 99.9 23
PDB header:hydrolase
Chain: A: PDB Molecule:lipj;
PDBTitle: mycobacterium tuberculosis adenylyl cyclase rv1900c chd, in complex2 with a substrate analog.
25c4wp3E_



not modelled 99.9 21
PDB header:lyase
Chain: E: PDB Molecule:ma1120;
PDBTitle: crystal structure of adenylyl cyclase from mycobacterium avium ma11202 wild type
26c2qv6D_



not modelled 97.2 13
PDB header:hydrolase
Chain: D: PDB Molecule:gtp cyclohydrolase iii;
PDBTitle: gtp cyclohydrolase iii from m. jannaschii (mj0145)2 complexed with gtp and metal ions
27c3hlsE_



not modelled 96.3 15
PDB header:signaling protein
Chain: E: PDB Molecule:guanylate cyclase soluble subunit beta-1;
PDBTitle: crystal structure of the signaling helix coiled-coil doimain2 of the beta-1 subunit of the soluble guanylyl cyclase
28c3i5aA_



not modelled 94.9 12
PDB header:signaling protein
Chain: A: PDB Molecule:response regulator/ggdef domain protein;
PDBTitle: crystal structure of full-length wpsr from pseudomonas syringae
29c3i5cA_



not modelled 94.4 15
PDB header:signaling protein
Chain: A: PDB Molecule:fusion of general control protein gcn4 and wspr response
PDBTitle: crystal structure of a fusion protein containing the leucine zipper of2 gcn4 and the ggdef domain of wspr from pseudomonas aeruginosa
30c5llxB_



not modelled 94.0 12
PDB header:transferase
Chain: B: PDB Molecule:diguanylate cyclase (ggdef) domain-containing protein;
PDBTitle: bacteriophytochrome activated diguanylyl cyclase from idiomarina2 species a28l with gtp bound
31c3breA_



not modelled 93.2 14
PDB header:signaling protein
Chain: A: PDB Molecule:probable two-component response regulator;
PDBTitle: crystal structure of p.aeruginosa pa3702
32c4zmuD_



not modelled 93.2 15
PDB header:lyase
Chain: D: PDB Molecule:diguanylate cyclase;
PDBTitle: dcsbis, a diguanylate cyclase from pseudomonas aeruginosa
33c4h54B_



not modelled 92.5 9
PDB header:transferase
Chain: B: PDB Molecule:diguanylate cyclase ydeh;
PDBTitle: crystal structure of the diguanylate cyclase dgcz
34c6d9mA_



not modelled 92.2 15
PDB header:hydrolase
Chain: A: PDB Molecule:fusion protein of endolysin,response receiver sensor
PDBTitle: t4-lysozyme fusion to geobacter ggdef
35c1w25B_



not modelled 89.5 13
PDB header:signaling protein
Chain: B: PDB Molecule:stalked-cell differentiation controlling protein;
PDBTitle: response regulator pled in complex with c-digmp
36c3i5bA_



not modelled 89.1 16
PDB header:signaling protein
Chain: A: PDB Molecule:wspr response regulator;
PDBTitle: crystal structure of the isolated ggdef domain of wpsr from2 pseudomonas aeruginosa
37c3ezuA_



not modelled 87.4 16
PDB header:signaling protein
Chain: A: PDB Molecule:ggdef domain protein;
PDBTitle: crystal structure of multidomain protein of unknown function with2 ggdef-domain (np_951600.1) from geobacter sulfurreducens at 1.95 a3 resolution
38c5xgdA_



not modelled 84.9 11
PDB header:transcription
Chain: A: PDB Molecule:uncharacterized protein pa0861;
PDBTitle: crystal structure of the pas-ggdef-eal domain of pa0861 from2 pseudomonas aeruginosa in complex with gtp
39c4ymeA_



not modelled 84.5 16
PDB header:lyase
Chain: A: PDB Molecule:sensory box/ggdef family protein;
PDBTitle: crystal structure of a sensory box/ggdef family protein (cc_0091) from2 caulobacter crescentus cb15 at 1.40 a resolution (psi community3 target, shapiro)
40c3ignA_



not modelled 82.0 8
PDB header:transferase
Chain: A: PDB Molecule:diguanylate cyclase;
PDBTitle: crystal structure of the ggdef domain from marinobacter aquaeolei2 diguanylate cyclase complexed with c-di-gmp - northeast structural3 genomics consortium target mqr89a
41c4urgB_



not modelled 81.4 16
PDB header:lyase
Chain: B: PDB Molecule:diguanylate cyclase;
PDBTitle: crystal structure of ggdef domain from t.maritima (active-like dimer)
42c4dmzB_



not modelled 81.1 12
PDB header:nucleotide-binding protein
Chain: B: PDB Molecule:putative uncharacterized protein peld;
PDBTitle: peld 156-455 from pseudomonas aeruginosa pa14, apo form
43c3hvwA_



not modelled 79.7 4
PDB header:lyase
Chain: A: PDB Molecule:diguanylate-cyclase (dgc);
PDBTitle: crystal structure of the ggdef domain of the pa2567 protein from2 pseudomonas aeruginosa, northeast structural genomics consortium3 target par365c
44c4wxoA_



not modelled 78.4 12
PDB header:transferase
Chain: A: PDB Molecule:uncharacterized protein;
PDBTitle: sadc (300-487) from pseudomonas aeruginosa pao1
45d1w25a3



not modelled 78.2 13
Fold:Ferredoxin-like
Superfamily:Nucleotide cyclase
Family:GGDEF domain
46c4iobA_



not modelled 78.1 18
PDB header:lyase
Chain: A: PDB Molecule:diguanylate cyclase tpbb;
PDBTitle: crystal structure of the ggdef domain of pa1120 (yfin or tpbb) from2 pseudomonas aeruginosa at 2.7 ang.
47c3mtkA_



not modelled 76.6 9
PDB header:transferase
Chain: A: PDB Molecule:diguanylate cyclase/phosphodiesterase;
PDBTitle: x-ray structure of diguanylate cyclase/phosphodiesterase from2 caldicellulosiruptor saccharolyticus, northeast structural genomics3 consortium target clr27c
48c5euhA_



not modelled 75.2 15
PDB header:membrane protein
Chain: A: PDB Molecule:putative ggdef domain membrane protein;
PDBTitle: crystal structure of the c-di-gmp-bound ggdef domain of p. fluorescens2 gcbc
49c5cupB_



not modelled 74.7 25
PDB header:transferase
Chain: B: PDB Molecule:phosphate propanoyltransferase;
PDBTitle: structure of rhodopseudomonas palustris pdul - phosphate bound form
50c3tvkA_



not modelled 74.1 9
PDB header:transferase
Chain: A: PDB Molecule:diguanylate cyclase dgcz;
PDBTitle: diguanylate cyclase domain of dgcz
51c4w8yA_



not modelled 72.7 13
PDB header:rna binding protein
Chain: A: PDB Molecule:crispr system cmr subunit cmr2;
PDBTitle: structure of full length cmr2 from pyrococcus furiosus (manganese2 bound form)
52c3ungC_



not modelled 72.4 11
PDB header:unknown function
Chain: C: PDB Molecule:cmr2dhd;
PDBTitle: structure of the cmr2 subunit of the crispr rna silencing complex
53c3hvaA_



not modelled 72.3 12
PDB header:transferase
Chain: A: PDB Molecule:protein fimx;
PDBTitle: crystal structure of fimx ggdef domain from pseudomonas aeruginosa
54c6eibC_



not modelled 71.6 16
PDB header:transferase
Chain: C: PDB Molecule:sensory box/ggdef family protein;
PDBTitle: structure of the active ggeef domain of a diguanylate cyclase from2 vibrio cholerae.
55c4zmmB_



not modelled 68.7 11
PDB header:transferase
Chain: B: PDB Molecule:diguanylate cyclase;
PDBTitle: ggdef domain of dcsbis complexed with c-di-gmp
56c4zvhB_



not modelled 68.0 18
PDB header:signaling protein
Chain: B: PDB Molecule:diguanylate cyclase dosc;
PDBTitle: crystal structure of ggdef domain of the e. coli dosc - form iv
57c5m3cB_



not modelled 63.1 13
PDB header:hydrolase
Chain: B: PDB Molecule:diguanylate cyclase;
PDBTitle: structure of the hybrid domain (ggdef-eal) of pa0575 from pseudomonas2 aeruginosa pao1 at 2.8 ang. with gtp and ca2+ bound to the active3 site of the ggdef domain
58c6ifnA_



not modelled 57.9 15
PDB header:rna binding protein
Chain: A: PDB Molecule:type iii-a crispr-associated protein csm1;
PDBTitle: crystal structure of type iii-a crispr csm complex
59c3pjwA_



not modelled 55.2 8
PDB header:lyase
Chain: A: PDB Molecule:cyclic dimeric gmp binding protein;
PDBTitle: structure of pseudomonas fluorescence lapd ggdef-eal dual domain, i23
60c3iclA_



not modelled 54.4 14
PDB header:structural genomics, unknown function
Chain: A: PDB Molecule:eal/ggdef domain protein;
PDBTitle: x-ray structure of protein (eal/ggdef domain protein) from2 m.capsulatus, northeast structural genomics consortium target mcr174c
61c2x5eA_



not modelled 53.0 20
PDB header:unknown function
Chain: A: PDB Molecule:upf0271 protein pa4511;
PDBTitle: crystal structure of the hypothetical protein pa4511 from2 pseudomonas aeruginosa
62d1v6ta_



not modelled 52.4 22
Fold:7-stranded beta/alpha barrel
Superfamily:Glycoside hydrolase/deacetylase
Family:LamB/YcsF-like
63c4euvA_



not modelled 48.8 11
PDB header:signaling protein
Chain: A: PDB Molecule:peld;
PDBTitle: crystal structure of peld 158-ct from pseudomonas aeruginosa pao1, in2 complex with c-di-gmp, form 1
64d2dfaa1



not modelled 48.5 24
Fold:7-stranded beta/alpha barrel
Superfamily:Glycoside hydrolase/deacetylase
Family:LamB/YcsF-like
65c3qyyB_



not modelled 48.5 12
PDB header:signaling protein/inhibitor
Chain: B: PDB Molecule:response regulator;
PDBTitle: a novel interaction mode between a microbial ggdef domain and the bis-2 (3, 5 )-cyclic di-gmp
66c4rnhA_



not modelled 24.4 13
PDB header:transferase, hydrolase
Chain: A: PDB Molecule:motility regulator;
PDBTitle: pamora tandem diguanylate cyclase - phosphodiesterase, c-di-gmp2 complex
67c5buzC_



not modelled 19.0 19
PDB header:transport protein
Chain: C: PDB Molecule:snap receptor-like protein;
PDBTitle: crystal structure of a complex between the snare vam3 and the hops2 vps33-vps16 subcomplex from chaetomium thermophilum
68d1m9dc_



not modelled 18.1 28
Fold:Retrovirus capsid protein, N-terminal core domain
Superfamily:Retrovirus capsid protein, N-terminal core domain
Family:Retrovirus capsid protein, N-terminal core domain
69c2xgvA_



not modelled 17.9 11
PDB header:viral protein
Chain: A: PDB Molecule:psiv capsid n-terminal domain;
PDBTitle: structure of the n-terminal domain of capsid protein from2 rabbit endogenous lentivirus (relik)
70c5t4oA_



not modelled 17.8 11
PDB header:hydrolase
Chain: A: PDB Molecule:atp synthase subunit alpha;
PDBTitle: autoinhibited e. coli atp synthase state 1
71c2pjhB_



not modelled 17.3 13
PDB header:transport protein
Chain: B: PDB Molecule:transitional endoplasmic reticulum atpase;
PDBTitle: strctural model of the p97 n domain- npl4 ubd complex
72d2eiaa2



not modelled 17.1 22
Fold:Retrovirus capsid protein, N-terminal core domain
Superfamily:Retrovirus capsid protein, N-terminal core domain
Family:Retrovirus capsid protein, N-terminal core domain
73d1m9fd_



not modelled 17.0 28
Fold:Retrovirus capsid protein, N-terminal core domain
Superfamily:Retrovirus capsid protein, N-terminal core domain
Family:Retrovirus capsid protein, N-terminal core domain
74d2pxrc1



not modelled 16.3 28
Fold:Retrovirus capsid protein, N-terminal core domain
Superfamily:Retrovirus capsid protein, N-terminal core domain
Family:Retrovirus capsid protein, N-terminal core domain
75c2wlvA_



not modelled 16.1 22
PDB header:viral protein
Chain: A: PDB Molecule:gag polyprotein;
PDBTitle: structure of the n-terminal capsid domain of hiv-2
76c2n5lA_



not modelled 15.7 15
PDB header:hydrolase
Chain: A: PDB Molecule:ribonuclease zc3h12a;
PDBTitle: regnase-1 c-terminal domain
77c4axkB_



not modelled 15.6 16
PDB header:isomerase
Chain: B: PDB Molecule:1-(5-phosphoribosyl)-5-((5'-phosphoribosylamino)
PDBTitle: crystal structure of subhisa from the thermophile corynebacterium2 efficiens
78c4rrfD_



not modelled 15.0 19
PDB header:ligase
Chain: D: PDB Molecule:threonine--trna ligase;
PDBTitle: editing domain of threonyl-trna synthetase from methanococcus2 jannaschii with l-ser3aa
79c5fa0B_



not modelled 14.1 18
PDB header:transferase
Chain: B: PDB Molecule:putative n-acetyl glucosaminyl transferase;
PDBTitle: the structure of the beta-3-deoxy-d-manno-oct-2-ulosonic acid2 transferase domain from wbbb
80c6fkhA_



not modelled 13.9 9
PDB header:membrane protein
Chain: A: PDB Molecule:atp synthase subunit alpha, chloroplastic;
PDBTitle: chloroplast f1fo conformation 2
81c2kjwA_



not modelled 13.3 18
PDB header:ribosomal protein
Chain: A: PDB Molecule:30s ribosomal protein s6;
PDBTitle: solution structure and backbone dynamics of the permutant p54-55
82c1avoA_



not modelled 13.3 33
PDB header:proteasome activator
Chain: A: PDB Molecule:11s regulator;
PDBTitle: proteasome activator reg(alpha)
83c5ggoB_



not modelled 13.0 24
PDB header:sugar binding protein
Chain: B: PDB Molecule:protein o-linked-mannose beta-1,2-n-
PDBTitle: crystal structure of n-terminal domain of human protein o-mannose2 beta-1,2-n-acetylglucosaminyltransferase in complex with galnac-3 beta1,3-glcnac-beta-pnp
84c2hl2A_



not modelled 12.7 19
PDB header:ligase
Chain: A: PDB Molecule:threonyl-trna synthetase;
PDBTitle: crystal structure of the editing domain of threonyl-trna2 synthetase from pyrococcus abyssi in complex with an3 analog of seryladenylate
85c3dcaC_



not modelled 12.4 13
PDB header:structural genomics, unknown function
Chain: C: PDB Molecule:rpa0582;
PDBTitle: crystal structure of the rpa0582- protein of unknown2 function from rhodopseudomonas palustris- a structural3 genomics target
86c6iqwA_



not modelled 12.3 16
PDB header:rna binding protein/rna
Chain: A: PDB Molecule:csm1;
PDBTitle: cryo-em structure of csm effector complex
87c2y0fD_



not modelled 12.1 21
PDB header:oxidoreductase
Chain: D: PDB Molecule:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase;
PDBTitle: structure of gcpe (ispg) from thermus thermophilus hb27
88c3a6mB_



not modelled 11.8 14
PDB header:chaperone
Chain: B: PDB Molecule:protein grpe;
PDBTitle: crystal structure of grpe from thermus thermophilus hb8
89c3nvbA_



not modelled 11.5 12
PDB header:structural genomics, unknown function
Chain: A: PDB Molecule:uncharacterized protein;
PDBTitle: crystal structure of n-terminal part of the protein bf1531 from2 bacteroides fragilis containing phosphatase domain complexed with mg3 and tungstate
90c2xgyA_



not modelled 11.1 17
PDB header:viral protein/isomerase
Chain: A: PDB Molecule:relik capsid n-terminal domain;
PDBTitle: complex of rabbit endogenous lentivirus (relik)capsid with2 cyclophilin a
91d1v4ea_



not modelled 11.1 16
Fold:Terpenoid synthases
Superfamily:Terpenoid synthases
Family:Isoprenyl diphosphate synthases
92c5dn6A_



not modelled 11.1 15
PDB header:hydrolase
Chain: A: PDB Molecule:atp synthase subunit alpha;
PDBTitle: atp synthase from paracoccus denitrificans
93c4aniA_



not modelled 10.8 13
PDB header:chaperone
Chain: A: PDB Molecule:protein grpe;
PDBTitle: structural basis for the intermolecular communication between2 dnak and grpe in the dnak chaperone system from3 geobacillus kaustophilus hta426
94c4tt1A_



not modelled 10.7 25
PDB header:hydrolase
Chain: A: PDB Molecule:deneddylase;
PDBTitle: crystal structure of fragment 1600-1733 of hsv1 ul36, native
95d2gsva1



not modelled 10.6 16
Fold:Open three-helical up-and-down bundle
Superfamily:YvfG-like
Family:YvfG-like
96c2vfwB_



not modelled 10.5 13
PDB header:transferase
Chain: B: PDB Molecule:short-chain z-isoprenyl diphosphate synthetase;
PDBTitle: rv1086 native
97d2crua1



not modelled 10.2 15
Fold:RuvA C-terminal domain-like
Superfamily:Double-stranded DNA-binding domain
Family:Double-stranded DNA-binding domain
98c1l6nA_



not modelled 10.2 28
PDB header:viral protein
Chain: A: PDB Molecule:gag polyprotein;
PDBTitle: structure of the n-terminal 283-residue fragment of the hiv-2 1 gag polyprotein
99d1k8ba_



not modelled 10.1 25
Fold:Ribosome binding domain-like
Superfamily:Translation initiation factor 2 beta, aIF2beta, N-terminal domain
Family:Translation initiation factor 2 beta, aIF2beta, N-terminal domain

Binding site prediction 

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction 

Transmembrane helices have been predicted in your sequence to adopt the topology shown below