1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	H	A	S	R	P	G	A	P	P	H	A	G	L	P	S	R	R	T	A	G	D	Q	D	H	R	A	D	P	K	V	T	R	I	M	S	A	S	T	L	E	Q	P	A	A	A	H	V	D	E	L	V	A	R	M	R	G	R	L	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	P	L	A	I	A	V	L	A	A	V	I	S	G	A	W	A	S	R	P	S	L	W	F	D	E	G	A	T	I	S	A	S	A	S	R	T	L	P	E	L	W	S	L	L	G	H	I	D	A	V	H	G	L	Y	Y	L	L	M	H
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	W	F	A	I	F	P	P	T	E	L	W	S	R	L	P	S	C	L	A	I	G	A	A	A	A	G	V	V	V	F	A	K	Q	F	S	G	R	T	T	A	V	C	A	G	A	V	F	A	I	L	P	R	V	T	W	A	G	I	E
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	R	S	S	A	L	S	V	A	A	A	V	W	L	T	V	L	L	V	A	A	V	R	C	N	T	Q	R	R	W	L	L	Y	A	L	V	L	M	L	S	I	L	V	S	I	N	L	A	L	L	V	P	A	Y	A	T	M	V	P	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	A	S	G	K	S	R	K	S	P	V	I	W	W	T	V	V	T	A	A	A	L	G	A	M	T	P	F	I	L	F	A	H	G	Q	V	W	Q	V	G	W	I	A	G	L	N	R	N	I	I	L	D	V	I	H	R	Q	Y	F	D
Secondary structure
SS confidence
Disorder					?	?	?	?	?																													?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		H	S	V	P	F	A	I	L	A	G	L	I	V	A	A	G	I	A	A	H	L	A	G	A	R	G	P	G	G	D	T	H	R	L	V	L	V	S	A	A	W	I	V	V	P	T	A	V	V	L	I	Y	S	A	T	V	E	P	I	Y
Secondary structure
SS confidence
Disorder																													?		?																								?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		Y	P	R	Y	L	I	L	T	A	P	A	A	A	V	I	L	A	V	C	V	V	T	I	A	R	K	P	W	L	I	A	G	V	V	F	L	L	A	A	A	A	F	P	N	Y	F	F	T	Q	R	G	P	Y	A	K	E	G	W	D	Y
Secondary structure
SS confidence
Disorder																																																	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		S	Q	V	A	D	V	I	S	A	H	A	K	P	G	D	C	L	L	V	D	N	T	A	G	W	R	P	G	P	I	R	A	L	L	A	T	R	P	A	A	F	R	S	L	I	D	V	E	R	G	T	Y	G	P	K	V	G	T	L	W
Secondary structure
SS confidence
Disorder																									?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		D	G	H	V	A	V	W	L	T	T	A	K	I	D	K	C	P	T	L	W	T	I	A	N	R	D	K	S	L	P	D	H	Q	V	G	E	M	L	S	P	G	T	G	F	G	R	T	P	V	Y	R	F	P	S	Y	L	G	F	R	I
Secondary structure
SS confidence
Disorder																														?		?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.
Sequence		V	E	R	W	Q	F	H	Y	S	Q	V	V	K	S	T	R
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 17%) Alpha helix ( 59%) Beta strand ( 2%) TM helix ( 38%) (View membrane topology prediction)

Rank Aligned region

PDB 5f15 chain A Region: 63 - 508 Aligned: 439 Modelled: 446 Confidence: 100.0% Identity: 14% PDB header:transferase Chain: A: PDB Molecule:4-amino-4-deoxy-l-arabinose (l-ara4n) transferase; PDBTitle: crystal structure of arnt from cupriavidus metallidurans bound to2 undecaprenyl phosphate Phyre2 PDB 6p2r chain B Region: 56 - 273 Aligned: 213 Modelled: 218 Confidence: 99.8% Identity: 13% PDB header:transferase Chain: B: PDB Molecule:dolichyl-phosphate-mannose--protein mannosyltransferase 2; PDBTitle: structure of s. cerevisiae protein o-mannosyltransferase pmt1-pmt22 complex bound to the sugar donor Phyre2 PDB 6p25 chain A Region: 20 - 274 Aligned: 245 Modelled: 255 Confidence: 99.8% Identity: 15% PDB header:transferase Chain: A: PDB Molecule:dolichyl-phosphate-mannose--protein mannosyltransferase 1; PDBTitle: structure of s. cerevisiae protein o-mannosyltransferase pmt1-pmt22 complex bound to the sugar donor and a peptide acceptor Phyre2 PDB 3waj chain A Region: 112 - 430 Aligned: 310 Modelled: 319 Confidence: 99.7% Identity: 14% PDB header:transferase Chain: A: PDB Molecule:transmembrane oligosaccharyl transferase; PDBTitle: crystal structure of the archaeoglobus fulgidus2 oligosaccharyltransferase (o29867_arcfu) complex with zn and sulfate Phyre2 PDB 3rce chain A Region: 106 - 440 Aligned: 321 Modelled: 335 Confidence: 99.7% Identity: 12% PDB header:transferase/peptide Chain: A: PDB Molecule:oligosaccharide transferase to n-glycosylate proteins; PDBTitle: bacterial oligosaccharyltransferase pglb Phyre2 PDB 6ezn chain F Region: 107 - 440 Aligned: 320 Modelled: 334 Confidence: 99.5% Identity: 16% PDB header:membrane protein Chain: F: PDB Molecule:dolichyl-diphosphooligosaccharide--protein PDBTitle: cryo-em structure of the yeast oligosaccharyltransferase (ost) complex Phyre2 PDB 2jfg chain A domain 2 Region: 418 - 446 Aligned: 29 Modelled: 29 Confidence: 79.5% Identity: 21% Fold: MurD-like peptide ligases, peptide-binding domain Superfamily: MurD-like peptide ligases, peptide-binding domain Family: MurCDEF C-terminal domain Phyre2 PDB 3uag chain A Region: 418 - 441 Aligned: 24 Modelled: 24 Confidence: 42.3% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:protein (udp-n-acetylmuramoyl-l-alanine:d- PDBTitle: udp-n-acetylmuramoyl-l-alanine:d-glutamate ligase Phyre2 PDB 3lk7 chain A Region: 418 - 441 Aligned: 24 Modelled: 24 Confidence: 37.3% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:udp-n-acetylmuramoylalanine--d-glutamate ligase; PDBTitle: the crystal structure of udp-n-acetylmuramoylalanine-d-glutamate2 (murd) ligase from streptococcus agalactiae to 1.5a Phyre2 PDB 3mvn chain A Region: 418 - 439 Aligned: 22 Modelled: 22 Confidence: 29.3% Identity: 32% PDB header:ligase Chain: A: PDB Molecule:udp-n-acetylmuramate:l-alanyl-gamma-d-glutamyl-medo- PDBTitle: crystal structure of a domain from a putative udp-n-acetylmuramate:l-2 alanyl-gamma-d-glutamyl-medo-diaminopimelate ligase from haemophilus3 ducreyi 35000hp Phyre2 PDB 1xq4 chain A Region: 497 - 539 Aligned: 43 Modelled: 42 Confidence: 27.7% Identity: 21% Fold: Immunoglobulin-like beta-sandwich Superfamily: ApaG-like Family: ApaG-like Phyre2 PDB 2f1e chain A Region: 498 - 539 Aligned: 42 Modelled: 42 Confidence: 27.5% Identity: 19% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:protein apag; PDBTitle: solution structure of apag protein Phyre2 PDB 5hdw chain A Region: 498 - 539 Aligned: 42 Modelled: 42 Confidence: 26.0% Identity: 24% PDB header:protein binding Chain: A: PDB Molecule:f-box only protein 3; PDBTitle: apag domain of fbxo3 Phyre2 PDB 1tza chain A Region: 497 - 539 Aligned: 43 Modelled: 42 Confidence: 25.7% Identity: 16% Fold: Immunoglobulin-like beta-sandwich Superfamily: ApaG-like Family: ApaG-like Phyre2 PDB 1xvs chain A Region: 497 - 539 Aligned: 43 Modelled: 42 Confidence: 25.0% Identity: 9% Fold: Immunoglobulin-like beta-sandwich Superfamily: ApaG-like Family: ApaG-like Phyre2 PDB 5n6m chain A Region: 201 - 515 Aligned: 289 Modelled: 300 Confidence: 19.9% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:apolipoprotein n-acyltransferase; PDBTitle: structure of the membrane integral lipoprotein n-acyltransferase lnt2 from p. aeruginosa Phyre2 PDB 1p3d chain A domain 2 Region: 418 - 439 Aligned: 22 Modelled: 22 Confidence: 18.7% Identity: 36% Fold: MurD-like peptide ligases, peptide-binding domain Superfamily: MurD-like peptide ligases, peptide-binding domain Family: MurCDEF C-terminal domain Phyre2 PDB 3hn7 chain A Region: 418 - 439 Aligned: 22 Modelled: 22 Confidence: 18.6% Identity: 27% PDB header:ligase Chain: A: PDB Molecule:udp-n-acetylmuramate-l-alanine ligase; PDBTitle: crystal structure of a murein peptide ligase mpl (psyc_0032) from2 psychrobacter arcticus 273-4 at 1.65 a resolution Phyre2 PDB 4buc chain A Region: 418 - 441 Aligned: 24 Modelled: 24 Confidence: 18.1% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:udp-n-acetylmuramoylalanine--d-glutamate ligase; PDBTitle: crystal structure of murd ligase from thermotoga maritima in apo form Phyre2 PDB 1e8c chain A domain 2 Region: 418 - 439 Aligned: 22 Modelled: 22 Confidence: 14.5% Identity: 23% Fold: MurD-like peptide ligases, peptide-binding domain Superfamily: MurD-like peptide ligases, peptide-binding domain Family: MurCDEF C-terminal domain Phyre2 1 c5f15A_ 2 c6p2rB_ 3 c6p25A_ 4 c3wajA_ 5 c3rceA_ 6 c6eznF_ 7 d2jfga2 8 c3uagA_ 9 c3lk7A_ 10 c3mvnA_ 11 d1xq4a_ 12 c2f1eA_ 13 c5hdwA_ 14 d1tzaa_ 15 d1xvsa_ 16 c5n6mA_ 17 d1p3da2 18 c3hn7A_ 19 c4bucA_ 20 d1e8ca2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99