1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	I	Y	R	V	A	C	L	L	A	R	I	R	F	T	V	G	Y	V	A	A	L	A	S	V	S	T	T	I	L	M	H	G	P	Q	V	H	A	Q	V	I	R	H	A	S	T	N	L	H	N	L	A	H	G	H	L	G	T	L	W
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		N	S	A	F	V	I	D	E	G	P	L	Y	F	W	L	P	C	L	A	C	L	L	A	V	A	E	L	Q	L	R	S	L	R	L	T	V	A	F	V	V	G	H	I	G	A	T	L	L	V	A	A	V	L	A	G	A	I	E	I	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		W	L	P	W	S	I	S	R	V	S	D	V	G	M	S	Y	G	A	L	A	A	L	G	A	L	T	A	A	I	P	G	R	W	R	P	A	W	I	G	W	W	V	S	L	G	L	A	T	A	T	I	G	G	G	F	T	D	A	G	H
Secondary structure
SS confidence
Disorder																																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		T	V	A	L	L	L	G	M	L	V	T	A	C	F	T	R	P	A	R	W	T	L	G	R	C	A	L	L	A	V	A	S	G	F	C	L	V	L	L	A	H	S	W	W	S	L	V	S	G	S	A	L	G	L	L	G	A	L	G	A
Secondary structure
SS confidence
Disorder																?			?	?	?	?	?																											?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.
Sequence		A	G	F	A	R	W	T	R	A	R	A	T	S	L	P	P	G	A	L	A	I	P	Q	P	A	L	S	R
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?	?	?			?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 10%) Alpha helix ( 81%) Beta strand ( 0%) TM helix ( 49%) (View membrane topology prediction)

Rank Aligned region

PDB 3b45 chain A domain 1 Region: 13 - 191 Aligned: 165 Modelled: 179 Confidence: 98.1% Identity: 13% Fold: Rhomboid-like Superfamily: Rhomboid-like Family: Rhomboid-like Phyre2 PDB 2nr9 chain A domain 1 Region: 10 - 196 Aligned: 170 Modelled: 173 Confidence: 97.7% Identity: 15% Fold: Rhomboid-like Superfamily: Rhomboid-like Family: Rhomboid-like Phyre2 PDB 3ky7 chain A Region: 187 - 201 Aligned: 15 Modelled: 15 Confidence: 7.4% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: 2.35 angstrom resolution crystal structure of a putative trna2 (guanine-7-)-methyltransferase (trmd) from staphylococcus aureus3 subsp. aureus mrsa252 Phyre2 PDB 5wyr chain A Region: 187 - 201 Aligned: 15 Modelled: 15 Confidence: 6.2% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure and catalytic mechanism of the essential m1g37 trna2 methyltransferase trmd from pseudomonas aeruginosa Phyre2 PDB 3ief chain A Region: 187 - 201 Aligned: 15 Modelled: 15 Confidence: 6.1% Identity: 40% PDB header:transferase, rna binding protein Chain: A: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure of trna guanine-n1-methyltransferase from bartonella2 henselae using mpcs. Phyre2 PDB 4h3z chain A Region: 187 - 201 Aligned: 15 Modelled: 15 Confidence: 5.7% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure of a symmetric dimer of a trna (guanine-(n(1)-)-2 methyltransferase from burkholderia phymatum bound to s-adenosyl3 homocystein in both half-sites Phyre2 PDB 1p9p chain A Region: 187 - 201 Aligned: 15 Modelled: 15 Confidence: 5.5% Identity: 33% Fold: alpha/beta knot Superfamily: alpha/beta knot Family: tRNA(m1G37)-methyltransferase TrmD Phyre2 PDB 1ual chain A Region: 187 - 201 Aligned: 15 Modelled: 15 Confidence: 5.4% Identity: 33% Fold: alpha/beta knot Superfamily: alpha/beta knot Family: tRNA(m1G37)-methyltransferase TrmD Phyre2 PDB 3quv chain B Region: 187 - 201 Aligned: 15 Modelled: 15 Confidence: 5.4% Identity: 33% PDB header:transferase Chain: B: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure of a trna-guanine-n1-methyltransferase from2 mycobacterium abscessus Phyre2 1 d3b45a1 2 d2nr9a1 3 c3ky7A_ 4 c5wyrA_ 5 c3iefA_ 6 c4h3zA_ 7 d1p9pa_ 8 d1uala_ 9 c3quvB_