1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	E	R	V	I	L	A	Y	S	G	G	L	D	T	S	V	A	I	S	W	I	G	K	E	T	G	R	E	V	V	A	V	A	I	D	L	G	Q	G	G	E	H	M	D	V	I	R	Q	R	A	L	D	C	G	A	V	E	A	V
Secondary structure
SS confidence
Disorder		?	?	?																																							?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	V	D	A	R	D	E	F	A	E	G	Y	C	L	P	T	V	L	N	N	A	L	Y	M	D	R	Y	P	L	V	S	A	I	S	R	P	L	I	V	K	H	L	V	A	A	A	R	E	H	G	G	G	I	V	A	H	G	C	T	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		K	G	N	D	Q	V	R	F	E	V	G	F	A	S	L	A	P	D	L	E	V	L	A	P	V	R	D	Y	A	W	T	R	E	K	A	I	A	F	A	E	E	N	A	I	P	I	N	V	T	K	R	S	P	F	S	I	D	Q	N	V
Secondary structure
SS confidence
Disorder																													?	?	?															?	?		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		W	G	R	A	V	E	T	G	F	L	E	H	L	W	N	A	P	T	K	D	I	Y	A	Y	T	E	D	P	T	I	N	W	G	V	P	D	E	V	I	V	G	F	E	R	G	V	P	V	S	V	D	G	K	P	V	S	M	L	A	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		I	E	E	L	N	R	R	A	G	A	Q	G	V	G	R	L	D	V	V	E	D	R	L	V	G	I	K	S	R	E	I	Y	E	A	P	G	A	M	V	L	I	T	A	H	T	E	L	E	H	V	T	L	E	R	E	L	G	R	F	K
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	Q	T	D	Q	R	W	A	E	L	V	Y	D	G	L	W	Y	S	P	L	K	A	A	L	E	A	F	V	A	K	T	Q	E	H	V	S	G	E	V	R	L	V	L	H	G	G	H	I	A	V	N	G	R	R	S	A	E	S	L	Y
Secondary structure
SS confidence
Disorder																																																									?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.
Sequence		D	F	N	L	A	T	Y	D	E	G	D	S	F	D	Q	S	A	A	R	G	F	V	Y	V	H	G	L	S	S	K	L	A	A	R	R	D	L	R
Secondary structure
SS confidence
Disorder							?	?	?	?	?	?	?	?	?																			?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 9%) Alpha helix ( 45%) Beta strand ( 17%)

Rank Aligned region

PDB 4u7j chain B Region: 3 - 398 Aligned: 389 Modelled: 396 Confidence: 100.0% Identity: 88% PDB header:ligase Chain: B: PDB Molecule:argininosuccinate synthase; PDBTitle: crystal structure of argininosuccinate synthase from mycobacterium2 thermoresistibile Phyre2 PDB 1vl2 chain C Region: 2 - 394 Aligned: 383 Modelled: 393 Confidence: 100.0% Identity: 40% PDB header:ligase Chain: C: PDB Molecule:argininosuccinate synthase; PDBTitle: crystal structure of argininosuccinate synthase (tm1780) from2 thermotoga maritima at 1.65 a resolution Phyre2 PDB 1kh2 chain D Region: 4 - 396 Aligned: 382 Modelled: 393 Confidence: 100.0% Identity: 42% PDB header:ligase Chain: D: PDB Molecule:argininosuccinate synthetase; PDBTitle: crystal structure of thermus thermophilus hb8 argininosuccinate2 synthetase in complex with atp Phyre2 PDB 1k97 chain A Region: 1 - 393 Aligned: 385 Modelled: 393 Confidence: 100.0% Identity: 28% PDB header:ligase Chain: A: PDB Molecule:argininosuccinate synthase; PDBTitle: crystal structure of e. coli argininosuccinate synthetase in complex2 with aspartate and citrulline Phyre2 PDB 2nz2 chain A Region: 2 - 394 Aligned: 390 Modelled: 393 Confidence: 100.0% Identity: 39% PDB header:ligase Chain: A: PDB Molecule:argininosuccinate synthase; PDBTitle: crystal structure of human argininosuccinate synthase in complex with2 aspartate and citrulline Phyre2 PDB 4nzp chain A Region: 3 - 398 Aligned: 379 Modelled: 396 Confidence: 100.0% Identity: 39% PDB header:ligase Chain: A: PDB Molecule:argininosuccinate synthase; PDBTitle: the crystal structure of argininosuccinate synthase from campylobacter2 jejuni subsp. jejuni nctc 11168 Phyre2 PDB 1j20 chain A domain 2 Region: 173 - 396 Aligned: 220 Modelled: 224 Confidence: 100.0% Identity: 39% Fold: Argininosuccinate synthetase, C-terminal domain Superfamily: Argininosuccinate synthetase, C-terminal domain Family: Argininosuccinate synthetase, C-terminal domain Phyre2 PDB 1vl2 chain A domain 2 Region: 171 - 396 Aligned: 218 Modelled: 226 Confidence: 100.0% Identity: 36% Fold: Argininosuccinate synthetase, C-terminal domain Superfamily: Argininosuccinate synthetase, C-terminal domain Family: Argininosuccinate synthetase, C-terminal domain Phyre2 PDB 1k92 chain A domain 2 Region: 173 - 393 Aligned: 221 Modelled: 221 Confidence: 100.0% Identity: 28% Fold: Argininosuccinate synthetase, C-terminal domain Superfamily: Argininosuccinate synthetase, C-terminal domain Family: Argininosuccinate synthetase, C-terminal domain Phyre2 PDB 1j20 chain A domain 1 Region: 4 - 167 Aligned: 163 Modelled: 164 Confidence: 100.0% Identity: 45% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 1k92 chain A domain 1 Region: 2 - 172 Aligned: 170 Modelled: 171 Confidence: 100.0% Identity: 29% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 1vl2 chain A domain 1 Region: 2 - 166 Aligned: 163 Modelled: 165 Confidence: 100.0% Identity: 46% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 3a2k chain B Region: 2 - 205 Aligned: 187 Modelled: 204 Confidence: 100.0% Identity: 16% PDB header:ligase/rna Chain: B: PDB Molecule:trna(ile)-lysidine synthase; PDBTitle: crystal structure of tils complexed with trna Phyre2 PDB 2e21 chain A Region: 2 - 204 Aligned: 186 Modelled: 190 Confidence: 100.0% Identity: 17% PDB header:ligase Chain: A: PDB Molecule:trna(ile)-lysidine synthase; PDBTitle: crystal structure of tils in a complex with amppnp from aquifex2 aeolicus. Phyre2 PDB 1ni5 chain A Region: 2 - 205 Aligned: 181 Modelled: 187 Confidence: 100.0% Identity: 18% PDB header:cell cycle Chain: A: PDB Molecule:putative cell cycle protein mesj; PDBTitle: structure of the mesj pp-atpase from escherichia coli Phyre2 PDB 2hma chain A Region: 2 - 235 Aligned: 224 Modelled: 231 Confidence: 100.0% Identity: 19% PDB header:transferase Chain: A: PDB Molecule:probable trna (5-methylaminomethyl-2-thiouridylate)- PDBTitle: the crystal structure of trna (5-methylaminomethyl-2-thiouridylate)-2 methyltransferase trmu from streptococcus pneumoniae Phyre2 PDB 3vrh chain A Region: 2 - 175 Aligned: 160 Modelled: 174 Confidence: 99.9% Identity: 18% PDB header:rna binding protein Chain: A: PDB Molecule:putative uncharacterized protein ph0300; PDBTitle: crystal structure of ph0300 Phyre2 PDB 5gha chain C Region: 2 - 172 Aligned: 160 Modelled: 171 Confidence: 99.9% Identity: 24% PDB header:transferase/transport protein Chain: C: PDB Molecule:sulfur transferase ttua; PDBTitle: sulfur transferase ttua in complex with sulfur carrier ttub Phyre2 PDB 1ni5 chain A domain 1 Region: 2 - 211 Aligned: 187 Modelled: 193 Confidence: 99.9% Identity: 17% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: PP-loop ATPase Phyre2 PDB 1wy5 chain A domain 1 Region: 2 - 172 Aligned: 155 Modelled: 171 Confidence: 99.9% Identity: 17% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: PP-loop ATPase Phyre2 1 c4u7jB_ 2 c1vl2C_ 3 c1kh2D_ 4 c1k97A_ 5 c2nz2A_ 6 c4nzpA_ 7 d1j20a2 8 d1vl2a2 9 d1k92a2 10 d1j20a1 11 d1k92a1 12 d1vl2a1 13 c3a2kB_ 14 c2e21A_ 15 c1ni5A_ 16 c2hmaA_ 17 c3vrhA_ 18 c5ghaC_ 19 d1ni5a1 20 d1wy5a1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289