1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		L	M	A	E	P	E	E	S	R	E	P	R	G	I	R	L	Q	K	V	L	S	Q	A	G	I	A	S	R	R	A	A	E	K	M	I	V	D	G	R	V	E	V	D	G	H	V	V	T	E	L	G	T	R	V	D	P	Q	V	A	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	R	V	D	G	A	R	V	V	L	D	D	S	L	V	Y	L	A	L	N	K	P	R	G	M	H	S	T	M	S	D	D	R	G	R	P	C	I	G	D	L	I	E	R	K	V	R	G	T	K	K	L	F	H	V	G	R	L	D	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		D	T	E	G	L	M	L	L	T	N	D	G	E	L	A	H	R	L	M	H	P	S	H	E	V	P	K	T	Y	L	A	T	V	T	G	S	V	P	R	G	L	G	R	T	L	R	A	G	I	E	L	D	D	G	P	A	F	V	D	D
Secondary structure
SS confidence
Disorder																						?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		F	A	V	V	D	A	I	P	G	K	T	L	V	R	V	T	L	H	E	G	R	N	R	I	V	R	R	L	L	A	A	A	G	F	P	V	E	A	L	V	R	T	D	I	G	A	V	S	L	G	K	Q	R	P	G	S	V	R	A	L
Secondary structure
SS confidence
Disorder																																																		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.
Sequence		R	S	N	E	I	G	Q	L	Y	Q	A	V	G	L
Secondary structure
SS confidence
Disorder													?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 7%) Alpha helix ( 25%) Beta strand ( 33%)

Rank Aligned region

PDB 3dh3 chain C Region: 11 - 253 Aligned: 233 Modelled: 243 Confidence: 100.0% Identity: 33% PDB header:isomerase/rna Chain: C: PDB Molecule:ribosomal large subunit pseudouridine synthase f; PDBTitle: crystal structure of rluf in complex with a 22 nucleotide rna2 substrate Phyre2 PDB 4lgt chain A Region: 14 - 254 Aligned: 237 Modelled: 239 Confidence: 100.0% Identity: 40% PDB header:isomerase/rna Chain: A: PDB Molecule:ribosomal large subunit pseudouridine synthase b; PDBTitle: crystal structure of the catalytic domain of rlub in complex with a2 21-nucleotide rna substrate Phyre2 PDB 1ksk chain A Region: 12 - 248 Aligned: 231 Modelled: 234 Confidence: 100.0% Identity: 35% PDB header:lyase Chain: A: PDB Molecule:ribosomal small subunit pseudouridine synthase a; PDBTitle: structure of rsua Phyre2 PDB 1vio chain A Region: 14 - 248 Aligned: 225 Modelled: 235 Confidence: 100.0% Identity: 32% PDB header:lyase Chain: A: PDB Molecule:ribosomal small subunit pseudouridine synthase a; PDBTitle: crystal structure of pseudouridylate synthase Phyre2 PDB 2ist chain A Region: 8 - 236 Aligned: 217 Modelled: 229 Confidence: 100.0% Identity: 24% PDB header:isomerase Chain: A: PDB Molecule:ribosomal large subunit pseudouridine synthase d; PDBTitle: crystal structure of rlud from e. coli Phyre2 PDB 2gml chain A Region: 73 - 252 Aligned: 171 Modelled: 180 Confidence: 100.0% Identity: 35% PDB header:isomerase Chain: A: PDB Molecule:ribosomal large subunit pseudouridine synthase f; PDBTitle: crystal structure of catalytic domain of e.coli rluf Phyre2 PDB 2oml chain A Region: 72 - 242 Aligned: 166 Modelled: 171 Confidence: 100.0% Identity: 33% PDB header:isomerase Chain: A: PDB Molecule:ribosomal large subunit pseudouridine synthase e; PDBTitle: crystal structure of e. coli pseudouridine synthase rlue Phyre2 PDB 2olw chain B Region: 73 - 242 Aligned: 165 Modelled: 170 Confidence: 100.0% Identity: 35% PDB header:isomerase Chain: B: PDB Molecule:ribosomal large subunit pseudouridine synthase e; PDBTitle: crystal structure of e. coli pseudouridine synthase rlue Phyre2 PDB 1ksk chain A domain 4 Region: 74 - 248 Aligned: 170 Modelled: 175 Confidence: 100.0% Identity: 36% Fold: Pseudouridine synthase Superfamily: Pseudouridine synthase Family: Pseudouridine synthase RsuA/RluD Phyre2 PDB 1vio chain A domain 1 Region: 72 - 248 Aligned: 170 Modelled: 177 Confidence: 100.0% Identity: 36% Fold: Pseudouridine synthase Superfamily: Pseudouridine synthase Family: Pseudouridine synthase RsuA/RluD Phyre2 PDB 1v9f chain A Region: 64 - 241 Aligned: 166 Modelled: 178 Confidence: 100.0% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:ribosomal large subunit pseudouridine synthase d; PDBTitle: crystal structure of catalytic domain of pseudouridine2 synthase rlud from escherichia coli Phyre2 PDB 1v9f chain A Region: 64 - 241 Aligned: 166 Modelled: 178 Confidence: 100.0% Identity: 21% Fold: Pseudouridine synthase Superfamily: Pseudouridine synthase Family: Pseudouridine synthase RsuA/RluD Phyre2 PDB 1qyu chain A Region: 64 - 236 Aligned: 161 Modelled: 173 Confidence: 100.0% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:ribosomal large subunit pseudouridine synthase d; PDBTitle: structure of the catalytic domain of 23s rrna pseudouridine2 synthase rlud Phyre2 PDB 1v9k chain A Region: 68 - 235 Aligned: 155 Modelled: 165 Confidence: 100.0% Identity: 26% Fold: Pseudouridine synthase Superfamily: Pseudouridine synthase Family: Pseudouridine synthase RsuA/RluD Phyre2 PDB 2i82 chain D Region: 64 - 236 Aligned: 159 Modelled: 172 Confidence: 100.0% Identity: 23% PDB header:lyase/rna Chain: D: PDB Molecule:ribosomal large subunit pseudouridine synthase a; PDBTitle: crystal structure of pseudouridine synthase rlua: indirect2 sequence readout through protein-induced rna structure Phyre2 PDB 5uba chain A Region: 69 - 235 Aligned: 155 Modelled: 166 Confidence: 100.0% Identity: 21% PDB header:rna binding protein Chain: A: PDB Molecule:rna pseudouridylate synthase domain-containing protein 4; PDBTitle: human rna pseudouridylate synthase domain containing 4 Phyre2 PDB 5vbb chain A Region: 62 - 237 Aligned: 154 Modelled: 176 Confidence: 100.0% Identity: 21% PDB header:rna binding protein Chain: A: PDB Molecule:rna pseudouridylate synthase domain-containing protein 1; PDBTitle: human rna pseudouridylate synthase domain containing 1 Phyre2 PDB 1vio chain A domain 2 Region: 14 - 70 Aligned: 56 Modelled: 57 Confidence: 99.1% Identity: 18% Fold: Alpha-L RNA-binding motif Superfamily: Alpha-L RNA-binding motif Family: Pseudouridine synthase RsuA N-terminal domain Phyre2 PDB 2apo chain A domain 2 Region: 76 - 231 Aligned: 143 Modelled: 156 Confidence: 99.0% Identity: 20% Fold: Pseudouridine synthase Superfamily: Pseudouridine synthase Family: Pseudouridine synthase II TruB Phyre2 PDB 2k6p chain A Region: 14 - 83 Aligned: 69 Modelled: 70 Confidence: 99.0% Identity: 19% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein hp_1423; PDBTitle: solution structure of hypothetical protein, hp1423 Phyre2 1 c3dh3C_ 2 c4lgtA_ 3 c1kskA_ 4 c1vioA_ 5 c2istA_ 6 c2gmlA_ 7 c2omlA_ 8 c2olwB_ 9 d1kska4 10 d1vioa1 11 c1v9fA_ 12 d1v9fa_ 13 c1qyuA_ 14 d1v9ka_ 15 c2i82D_ 16 c5ubaA_ 17 c5vbbA_ 18 d1vioa2 19 d2apoa2 20 c2k6pA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99